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Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR

  • Wang, Yanhua;Wang, Guangxiang;Zhang, Delin;Yin, Hong;Wang, Meng
    • Parasites, Hosts and Diseases
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    • v.51 no.5
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    • pp.573-577
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    • 2013
  • A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

Is Real Appreciation or More Government Debt Contractionary? The Case of the Philippines

  • Hsing, Yu;Morgan, Yun-Chen
    • East Asian Journal of Business Economics (EAJBE)
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    • v.4 no.4
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    • pp.1-7
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    • 2016
  • This paper has studied the impacts of the exchange rate, government debt as a percent of GDP and other relevant macroeconomic variables on aggregate output in the Philippines. A simultaneous-equation model consisting of aggregate demand and short-run aggregate supply is applied. The dummy variable technique is employed to detect whether the slope and intercept of the real effective exchange rate may have changed. Real depreciation during 1998.Q1 - 2006.Q3, real appreciation during 2006.Q4 - 2016.Q1, a lower domestic debt as a percent of GDP, a lower real interest rate, a higher stock price or a higher lagged real oil price would raise aggregate output. Recent trends of real peso appreciation, declining domestic debt as a percent of GDP, lower real interest rates, and rising stock prices are in line with the empirical results and would promote economic growth. The authorities may need to continue to pursue fiscal prudence and maintain a stronger peso as the positive effect of real appreciation dominates its negative effect in recent years.

A Study on the Type of Attitude toward the Sexual Violence (성폭력에 대한 태도 유형)

  • Nam, Soon-Yeul;Yoo, Eun-Kwang;Park, Kyung-Eun
    • Women's Health Nursing
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    • v.6 no.1
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    • pp.5-17
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    • 2000
  • The purpose of this study is to identify the type of attitude toward the sexual violence and to analyze the structure of subjectivity about the sexual violence. Q-methodological method was used. The Q concourse for this study was formed through literature review, open question and in-depth interview, 171 Q statements were obtained and finally 33 statements were selected. The subjects for Q classifications were 20 persons. The Quanl PC program was used for analysis. The results of the study were as follows : The first type, blaming offenders consisted of 10 subjects has the recognition that victims must not be blamed ; offenders are responsible for any circumstances ; and sexual violence is an infringement of human rights. the second type, blaming victims consisted of 3 subjects has the recognition that victims carelessness is causal factor of sexual violence. They recognized the sexual violence as an identical concept with sexual intercourse. The third type, blaming social moral consisted of 7 subjects has the recognition that the collapse of social ethics and moral gives rise to sexual violence. They recognized the sexual violence as both sexual intercourse and the infringement of human rights. In conclusion, the result leads to understand the type of attitude toward sexual violence in Korean sociocultural reality. Consequently, the results will be conductive to provide guidelines for nursing intervention and education program for preventing sexual violence.

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Gain Tuning for SMCSPO of Robot Arm with Q-Learning (Q-Learning을 사용한 로봇팔의 SMCSPO 게인 튜닝)

  • Lee, JinHyeok;Kim, JaeHyung;Lee, MinCheol
    • The Journal of Korea Robotics Society
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    • v.17 no.2
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    • pp.221-229
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    • 2022
  • Sliding mode control (SMC) is a robust control method to control a robot arm with nonlinear properties. A high switching gain of SMC causes chattering problems, although the SMC allows the adequate control performance by giving high switching gain, without the exact robot model containing nonlinear and uncertainty terms. In order to solve this problem, SMC with sliding perturbation observer (SMCSPO) has been researched, where the method can reduce the chattering by compensating the perturbation, which is estimated by the observer, and then choosing a lower switching control gain of SMC. However, optimal gain tuning is necessary to get a better tracking performance and reducing a chattering. This paper proposes a method that the Q-learning automatically tunes the control gains of SMCSPO with an iterative operation. In this tuning method, the rewards of reinforcement learning (RL) are set minus tracking errors of states, and the action of RL is a change of control gain to maximize rewards whenever the iteration number of movements increases. The simple motion test for a 7-DOF robot arm was simulated in MATLAB program to prove this RL tuning algorithm. The simulation showed that this method can automatically tune the control gains for SMCSPO.

Electrical Properties of Multilayer Piezoelectric Transformer using PMN-PZN-PZT Ceramics (PMN-PZN-PZT 세라믹스를 이용한 적층형 압전변압기의 전기적 특성)

  • Lee, Chang-Bae;Yoo, Ju-Hyun;Paik, Dong-Soo;Kang, Jin-Kyu;Cho, Hong-Hee;Lee, Sung-Ill
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.7
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    • pp.655-661
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    • 2006
  • Dielectric and piezoelectric properties of PMN-PZT ceramics with a high mechanical quality factor$(Q_m)$ and a low temperature sintering temperature were investigated as a function of PZN substitution in order to develop multilayer piezoelectric transformer for AC-DC converter. Multilayer piezoelectric transformers were subsequently manufactured using the PMN-PZN-PZT ceramic offering the optimal behavior and then the electrical performance were invetigated. At the sintering temperature of $940^{\circ}C$, density, electromechanical coupling factor$(k_p)$, mechanical qualify factor$(Q_m)$ and dielectric constant$(\varepsilon_r)$ of 8 mol% PZN substituted specimen were $7.73g/cm^3$, 0.524, 1573 and 1455, respectively. The PZN substitution caused a increase in the dielectric constant and the electromechnical coupling factor. The voltage step-up ratio of multilayer piezoelectric transformer showed the maximum value at near the resonant frequency of 76.55 kHz and increased according to the increase of load resistance. The multilayer piezoelectric transformer with the output impedance coincided with the load resistance showed the temperature increase of less than $20^{\circ}C$ at the output power of 10 W. Based on the results, the manufactured multilayer transformer using the low temperature sintered PMN-PZN-PZT ceramics can be stably driven for both step-up and down transformers.

Development of Looped Rating Curve at Hwawon Station (화원 수위관측소 지점의 고리모양 수위-유량 관계곡선 작성)

  • Kang Shin-Uk;Lee Sang-Ho;Hwang Man-Ha
    • Journal of Korea Water Resources Association
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    • v.39 no.6 s.167
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    • pp.487-494
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    • 2006
  • The flood discharge on the rising limb of a hydrograph at Hwawon station greatly differs from the flood discharge on the falling limb for the same stage. When there is such a big hysteresis, there can be a significant amount of errors in the rated discharge obtained from a simple rating curve. To reduce errors in rated discharges, a looped rating curve was established for Hwawon station in the Nakdong River. In order to compute the deviation between real discharges and simply rated discharges, a simple rating curve was established using the stage and discharge data from the results of a hydraulic channel routing. The relationship between the discharge deviation ${\Delta}Q$ and a product of B and ${\Delta}h/{\Delta}t$ was analysed, where B is the channel topwidth; ${\Delta}h$ is the stage increment; At is the time increment. Strong relation between ${\Delta}Q$ and $B{\Delta}h/{\Delta}t$ was found. The discharges calculated from the relationship show differences by 10 % or less for the 7 observations out of 11 observations in 1997 whose stages exceeds 7 m. The observed discharges for the stages over 9 m in 1998 also show small difference with the discharges estimated from the loop rating curve. Looped rating curve is recommended, instead of the simple rating curve to reduce the errors of rated discharges for gauging stations like Hwawon, which has relatively large loop width.

Estimation of Pollutant Load Delivery Ratio for Flow Duration Using L-Q Equation from the Oenam-cheon watershed in Juam Lake (유량-부하량관계식을 이용한 주암호 외남천 유역의 유황별 유달율 산정)

  • Choi, Dong-Ho;Jung, Jae-Woon;Lee, Kyoung-Sook;Choi, Yu-Jin;Yoon, Kwang-Sik;Cho, So-Hyun;Park, Ha-Na;Lim, Byung-Jin;Chang, Nam-Ik
    • Journal of Environmental Science International
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    • v.21 no.1
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    • pp.31-39
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    • 2012
  • The objective of this study is to provide pollutant loads delivery ratio for flow duration in Oenam-cheon watershed, which is upstream watershed of Juam Lake. To calculate the delivery ratio by flow duration, rating curves and discharge-loads curves using measured data were established, then Flow Duration Curve(FDC) and pollutant loads delivery ratio curves were constructed. The results show that the delivery ratios for $BOD_5$ for abundant flow($Q_{95}$), ordinary flow($Q_{185}$), low flow($Q_{275}$), and drought flow($Q_{355}$) were 23.9, 12.7, 7.1, and 2.9%, respectively. The delivery ratios of same flow regime for T-N were 58.4, 31.2, 17.2 and 7.1%, respectively. While, the delivery ratios T-P were 17.3, 7.5, 3.4, and 1.1% respectively. In general, delivery ratio of high flow condition showed higher value due to the influence of nonpoint source pollution. Based on the study results, generalized equations were developed for delivery ratio and discharge per unit area, which could be used for ungaged watershed with similar pollution sources.

Subjectivity study of Cooking Major College Student according to Cooking Practice Subject's Untact Online Class -Focusing on using Google Classroom- (조리실습과목의 비대면 온라인수업에 따른 조리전공 대학생의 주관성 연구 -구글 클래스룸 활용 중심-)

  • Lee, Kang-Yeon;Kim, Chan-Woo
    • The Journal of the Korea Contents Association
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    • v.21 no.4
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    • pp.292-302
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    • 2021
  • The purpose of this study was to use Google Classroom for Cooking Practice Subject. We would like to present a practice class operation method suitable for the current educational environment and conditions. If the Untact Class in the Practice Subject is conducted in the future, we want to cultivate the core competencies and practical skills of the major. In addition, Q Methodology, which can be extracted by type, was applied by analyzing subjective opinions or perception structures for the cooking major students who are currently experiencing the curriculum. The survey period was conducted from March 23 to April 30, 2020 for first-year Cooking Major students taking the Cooking Practice Subject. Type 1 (N=11): Development of cooking training kit, Type 2 (N=7): Special lectures from industry experts, Type 3 (N=7): Development of practice form self-directed learning, respectively. Based on this study, it is expected to contribute to the Q Methodology on the development of the curriculum for the operation of the cooking practice subject, the effectiveness of education, and the application of learning methods.

Chemical Constituents of Domestic Quercus spp. Leaves (국내산 참나무속 수종 잎의 추출성분)

  • Kim, Jin-Kyu;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.6
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    • pp.61-71
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    • 2006
  • This study was carried out to investigate chemotaxonomical correlation an d the chemical constituents of domestic Quercus sp. leaves. The leaves of Q. mongolica, Q. aliena, Q. serrata, Q. acutissima, Q. dentata and Q. variabilis were collected in the experimental forest of Kangwon National University. The combined extracts were successively fractionated with n -hexane, methylene chloride and ethyl acetate using a separation funnel. A portion of the ethyl acetate and $H_2O$ soluble materials of each species were chromatographed on a Sephadex LH-20 column using various aqueous MeOH and EtOH-hexane as washing solvents. Spectrometric analysis such as NMR and MS, including TLC, were performed to characterize the structures of the isolated compounds. Gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, kaempferol, astragalin, astragalin-6"-O-gallate, isoquercitrin, isoquercitrin-6"-O-gallate and myricetin were isolated from Q. mongolic a leaves. Gallic acid, kaempferol and quercetin were characterized from Q. acutissima leaves. Gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, (-)-epigallocatechin, kaempferol, quercetin, guajaverin and tamarixin were identified from Q. dentata leaves. Gallic acid, (+)-catechin, (-)-epicatechin, kaempferol, quercitrin, isoquercitrin and myricetin were purified from Q. serrata leaves. Gallic acid, (+)-catechin, astragalin, astragalin-6"-O-gallate and isoquercitrin were isolated from Q. variabilis leaves. Gallic acid was isolated from all the leaves and could be a taxonomic index on Quercus spp..

Groundwater evaluation in the Bokha watershed of the Namhan River using SWAT-MODFLOW (SWAT-MODFLOW를 활용한 남한강 복하천유역의 지하수 모의 평가)

  • Han, Daeyoung;Lee, Jiwan;Jang, Wonjin;Kim, Seongjoon
    • Journal of Korea Water Resources Association
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    • v.53 no.11
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    • pp.985-997
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    • 2020
  • SWAT (Soil and Water Assessment Tool)-MODFLOW (Modular Groundwater Flow) is a coupled model that linking semi-distributed watershed hydrology with fully-distributed groundwater behavior. In this study, the groundwater simulation results of SWAT and SWAT-MODFLOW were compared for Bokhacheon watershed in Namhan river basin. The models were calibrated and validated with 9 years (2009~2017) daily streamflow (Q) data of Heungcheon (HC) water level gauge station and the daily groundwater level observation data of Yulheon (YH). For SWAT, the groundwater parameters of GW_DELAY, GWQMN, and ALPHA_BF affecting baseflow and recession phase were treated. The SWAT results showed the coefficient of determination (R2) of 0.7 and Nash-Sutcliffe model efficiencies (NESQ, NSEinQ) for Q and 1/Q with 0.73 and -0.1 respectively. For SWAT-MODFLOW, the spatio-temporal aquifer hydraulic conductivity (K, m/day), specific storage (Ss, 1/m), and specific yield (Sy) were applied. The SWAT-MODFLOW showed R2, NSEQ, and NSEinQ of 0.69, 0.74, and 0.51 respectively. The SWAT-MODFLOW considerably enhanced the low flow simulation with the help of aquifer physical information. The total streamflow of SWAT and SWAT-MODFLOW were 718.6 mm and 854.9 mm occupying baseflow of 342.9 mm and 423.5 mm respectively.