• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1419-1424
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• 2024

Secretin PilQ is an antigenically conserved outer membrane protein that is present in most meningococci and PorA is a major protein that elicits bactericidal immune response in humans following natural disease and immunization. In the present study, BALB/c mice were immunized subcutaneously with rPilQ406-770 or rPorA together with Freund's adjuvant (FA). Serum antibody responses to serogroup A and B Neisseria meningitides whole cells or purified proteins and functional activity of antibodies were determined by ELISA and serum bactericidal assay (SBA), respectively. Serum IgG responses were significantly increased in the immunized group with rPilQ406-770 or rPorA together with FA compared to control groups. IgG antibody response of mice immunized with rPilQ406-770 was significantly more than mice immunized with rPorA (OD at 450 nm was 1.6 versus 0.83). The booster injections were effective in increasing the responses of antirPilQ406-770 or anti-rPorA IgG significantly. Antisera produced against rPilQ406-770 or rPorA demonstrated strong surface reactivity to serogroup B N. meningitides in comparison with control groups. Antisera raised against rPorA or rPilQ406-770 and FA demonstrated SBA titers from 1/1024 to 1/2048 against serogroup B. The strongest bactericidal activity was detected in sera from mice immunized with rPilQ406-770 mixed with FA. These results suggest that rPilQ406-770 is a potential vaccine candidate for serogroup B N. meningitidis.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.2D
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• pp.191-203
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• 2008

According to a characteristic analysis of traffic accidents of industrial vehicles (RTSA 2007), the occurrence rate and lethality per 10,000 vehicles is 6.7 times higher and 5.3 times higher than that of non-industrial vehicles respectively. For the recent 10 years, in contrast to the 1.5% decrease of the annual rate of entire traffic accidents, the accident rate of industrial vehicles have been increasing 2.0% on the annual average. For the recent 10 years, the accidents of a freight truck and bus have been steadily falling off. But, in the case of a taxi, it has constantly been rising 5.6%. In these situations, the countermeasures to decrease the accident rate are suggested by grasping the inclination of taxi drivers through Q analysis which is character and psychology analysis method.

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.220-231
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• 2006

The study was conducted to investigate the differences in carbon storage of Quercus mongolica stands with respect to latitude and altitude in Korea. Study sites were located in Mt. Joongwang, Pyeongchang-gun, Gangwon-do (altitudes 1,300 m, 1,000 m, and 800 m), Mt. Taehwa, Gwangju-si, Gyeonggi-do (altitude 350 m), Mt. Wolak, lecheon-si, Chungcheongbuk-do (altitude 300 m), Mt. Baekwoon, Gwangyang-si, Jeollanam-do (altitude 800 m), and Mt. Halla, Jeju-do (altitude 1,000 m). Total carbon storage and annual carbon storage of Q. mongolica stands were 85-210 tonC/ha and 7.2-10.6 tonC/ha, respectively. Lower latitude (NE) stands of Q. mongolica showed more carbon storage and annual carbon storage than higher latitude stands. Carbon storage and annual carbon storage of Q. mongolica stands were increased in low altitude. Carbon storage of Q. mongolica stands was higher in the northern aspect than in the southern aspect. However, there were no significant differences in annual carbon storage between the aspects.

• Journal of the Korean Mathematical Society
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• v.45 no.5
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• pp.1379-1391
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• 2008

Let k be an imaginary quadratic field, ${\eta}$ the complex upper half plane, and let ${\tau}{\in}{\eta}{\cap}k,\;q=e^{{\pi}{i}{\tau}}$. For n, t ${\in}{\mathbb{Z}}^+$ with $1{\leq}t{\leq}n-1$, set n=${\delta}{\cdot}2^{\iota}$(${\delta}$=2, 3, 5, 7, 9, 13, 15) with ${\iota}{\geq}0$ integer. Then we show that $q{\frac}{n}{12}-{\frac}{t}{2}+{\frac}{t^2}{2n}{\prod}_{m=1}^{\infty}(1-q^{nm-t})(1-q^{{nm}-(n-t)})$ are algebraic numbers.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.601-610
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• 2021

The tidal current patterns at Keum River Estuary before and after the construction of coastal structures were compared according to the CASES. The depth-integrated and tidal difference treatment applied FLOW2DH numerical model was used for the tidal current predictions. The test conditions consisted of before construction of coastal structures (CASE1), after construction of coastal structures (CASE2), and the addition of watergate operation(CASE1Q and CASE2Q), and present (CASE3). CASE1 showed a stable tidal current pattern, such as a natural estuary. In CASE2, the tidal current velocities and directions of the Keum River Estuary were changed due to the installed coastal structures. In particular, the tidal current velocities of the Gaeya open channel sections (P5~P9) in CASE2 were calculated to be 10~30% larger than that of CASE1. In the case of the Gunsan Inner Harbor (P4), which is closest to the Geum River Estuary, the ebb flow rate was approximately 250~300% faster than that of other CASEs due to the discharge of the watergate operation for 2.7 hours during the ebb of CASE1Q and CASE2Q. This will affect sediment transport, and it is predicted to lead to seabed changes. CASE3 is considered to be entering the stabilization stage according to the simulation of the tidal current velocities and directions of the Keum River Estuary and the surrounding coastal area.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.7
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• pp.3425-3430
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• 2013

The fast-growing market in wireless communications has led to the development of multi-standard mobile terminals. In this paper, a frequency-tunable active RC bandpass filter for multi-standards wireless communication system is designed using an active inductor. The conventional bandpass filter design methods employ the high order filter or high quality factor Q to improve the stopband attenuation characteristics and frequency selectivity of the passband. The proposed bandpass filter based on the high Q active inductor has an improved frequency characteristics. The center frequency and gain of the designed bandpass filter is tuned by employing the tuning circuit. We have performed the simulation using TSMC $0.18{\mu}m$ process parameter to analyze the characteristics of the designed active RC bandpass filter. The bandpass filter with Q=20.5 has 90MHz half power bandwidth at the center frequency of 1.86GHz. Moreover, the center frequency of the proposed bandpass filter can be tuned between 1.86~2.38GHz for the multi-standards wireless communication system using the capacitor of the tuning circuit.

• The Korean Journal of Ecology
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• v.16 no.3
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• pp.353-364
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• 1993

To elucidate ecophysiological factors affecting nutural distribution of evergreen broad-leabed trees in Danohad National Marine Park, water potential, relative water content and photosynthetic activities of 4 species, Cammellia japonica, Machilus thunbergii, Castanopsis cuspidata var.sieboldii and Quercus acuta, were potential began to decrease from 07:00h to its minimum value at 13:00h for C. cuspidata var. sieboldii (-14.3bar) and Q. acuta (-19.4bar) at 16:00 h for M. thunbergii(-17.0bar) and at 19:00 h for C. japonica (-14.5bar), and these showed similar trends to relative water content. Photosynthetic activities of 4 species began to increase from 7:00 h and reached maximum values before their minimum values of water potential occureed. Optimum temperature renges of photosynthetic activities was $18~20^{\circ}C$ for C. japonica - M. thunbergii and $14~16^{\circ}C$ for C. cuspidata var. sieboldii Quercus acuta.Q.acuta had the highest light saturation point of $0.4mM/m^2/s$ and C. japonica the lowest of $0.15mM/m^2/s$. Water potential of 4 species subjected to water stress, began to decrease after 1st day of drought and after 21th day of drought, those of C. japonica, M. thunbergii, C. cuspidata var. sieboldii and Q. acuta decreased to species began to decrease after 7th day of drought and after 21th day, those of M. thunbergii, C. cuspidata var. sieboldii and Q. acuta were dropped to about 50% and C. japonica 83.5%. Photosynthetic activity of 4 specie began to decline in the order of C. cuspidata var. sieboldii, Q.acuta, C. japonica and M. thunbergii after 10th day of drought. These results suggest that the segregated disributions of C. japonica - M. thunbergii on vally positions and C. cuspidata var. sieboldii - Q. acuta on slopes were associated with different responses of water status and photosyntheties to their environment.

• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1859-1864
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• 2018

Synthesis of flavonoid glycoside is difficult due to diverse hydroxy groups in flavonoids and sugars. As such, enzymatic synthesis or biotransformation is an approach to solve this problem. In this report, we used stepwise biotransformation to synthesize two quercetin bisglycosides (quercetin 3-O-glucuronic acid 7-O-rhamnoside [Q-GR] and quercetin 3-O-arabinose 7-O-rhamnoside [Q-AR]) because quercetin O-rhamnosides contain antiviral activity. Two sequential enzymatic reactions were required to synthesize these flavonoid glycosides. We first synthesized quercetin 3-O-glucuronic acid [Q-G], and quercetin 3-O-arabinose [Q-A] from quercetin using E. coli harboring specific uridine diphopsphate glycosyltransferase (UGT) and genes for UDP-glucuronic acid and UDP-arabinose, respectively. With each quercetin 3-O-glycoside, rhamnosylation using E. coli harboring UGT and the gene for UDP-rhamnose was conducted. This approach resulted in the production of 44.8 mg/l Q-GR and 45.1 mg/l Q-AR. This stepwise synthesis could be applicable to synthesize various natural product derivatives in case that the final yield of product was low due to the multistep reaction in one cell or when sequential synthesis is necessary in order to reduce the synthesis of byproducts.

• Korean Journal of Optics and Photonics
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• v.20 no.5
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• pp.276-280
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• 2009

A Cr:YAG crystal was used as a saturable absorber for passive Q-switching of a Nd:$YVO_4$ laser which was pumped by a 1-W continuous wave laser diode. The first surface of the Cr:YAG was high-reflection coated for the pump wavelength. The high-reflection coating improved the absorption efficiency of the pump beam in the Nd:$YVO_4$ through double pass absorption. It also prevented pump beam induced partial bleaching of the Cr:YAG. The peak-to-peak pulse fluctuation of passively Q-switched laser output was approximately 4 %. The minimum pulse-width was measured to be 7.11 ns. Also, the average pulse repetition rate was 9 kHz and the maximum output power was 16.27 mW.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.5
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• pp.1023-1028
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• 2012

A MMIC (Monolithic Microwave Integrated Circuit) mixer chip using the Schottky diode of an GaAs p-HEMT process has been developed for the I/Q demodulator of non-contact near field microwave probing system. A single balanced mixer type is adopted to achieve simple structure of the I/Q demodulator. A quadrature hybrid coupler and a quarter wavelength transmission line for 180 degree hybrid are realized with lumped elements of MIM capacitor and spiral inductor to reduce the mixer chip size. According to the on-wafer measurement, this MMIC mixer covers RF and LO frequencies of 1650MHz to 2050MHz with flat conversion loss. The MMIC mixer with miniature size of $2.5mm{\times}1.7mm$ demonstrates conversion loss below 12dB for both variations of RF and LO frequencies, LO-to-IF isolation above 43dB and RF-to-IF isolation above 23dB, respectively.