• 한국산림과학회지
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• 제110권3호
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• pp.431-439
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• 2021

본 연구에서 잣 구과피의 3가지 추출법(에탄올 추출, 초임계 추출, 에탄올과 초임계 추출물의 혼합)에 따른 항산화 활성, 주름개선 그리고 미백활성의 차이를 확인하였다. 그 결과, 총 폴리페놀 함량은 초임계 추출물이 11.03 mg/g GAE, 70% 에탄올 추출물이 33.79 mg/g GAE이었다. 전자공여능의 경우 1,000 ㎍/mL 농도에서 초임계 추출물은 13.60%, 70% 에탄올 추출물은 91.37%, 혼합물은 71.62%이었다. SOD 유사활성은 100 ㎍/mL 농도에서 초임계 추출물은 16.49%, 에탄올 추출물은 21.84%, 혼합물은 10.7%이었다. ABTS⁺ radical 소거능은 1,000 ㎍/mL 농도에서 초임계 추출물은 38.19%, 70% 에탄올 추출물은 80.23%, 혼합물은 78.72% 이었다. Tyrosinase 저해활성은 1,000 ㎍/mL 농도에서 초임계 추출물은 24.54%, 70% 에탄올 추출물은 36.55%, 혼합물은 15.23%이었다. Elastase 저해활성 측정은 1,000 ㎍/mL 농도에서 초임계 추출물은 15.62%, 70% 에탄올 추출물은 22.56%, 혼합물은 26.64%이었다. 피부수렴활성 결과는 70% 에탄올 추출물이 5000 ㎍/mL에서 81.23%이었고, 초임계 추출물과 혼합물은 활성을 나타내지 않았다. 본 연구에서 분석한 활성 결과는 잣구과피 70% 에탄올 추출물, 혼합물, 초임계 추출물 순으로 우수하게 측정되어 총 폴리페놀 함량 및 항산화 활성 모두 초임계 추출물이 높을 것으로 판단한 가설과 정반대의 결과를 나타내었다.

• 한국식품영양학회지
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• 제34권2호
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• pp.181-186
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• 2021

Antioxidant properties and antioxidant activities were analyzed for water extracts and 50% and 70% ethanol extracts of the leaf of Angelica gigas Nakai. The polyphenol and flavonoid contents in water, 50%, and 70% ethanol extract of the leaf of Angelica gigas Nakai, it was found that the polyphenol contents were 18.75 mg GAE/g, 28.95 mg GAE/g, and 34.73 mg GAE/g, respectively, and flavonoid contents were respectively. The DPPH IC50 scavenging activity was 45.84 mg/mL, 36.44 mg/mL, 19.11 mg/mL, respectively, and theABTS+ radical scavenging ability (1 mg/mL) was 28.73%, 22.79%, and 12.70%, respectively. Tyrosinase inhibitory activity, 70% ethanol extract, 50% ethanol extract, and water extract 33.14%, and 4.53%, respectively. Nitrite scavenging activity, 70% ethanol extract, 50% ethanol extract, and water extract were in the order of 1 mg/mL scavenging activity, 36.43%, 34.80%, and 18.85%, respectively.

• 한국식품영양학회지
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• 제30권1호
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• pp.51-58
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• 2017

Antioxidant activities of 80% methanol, water, and 70% ethanol extracts of Lentinula edodes GNA01 were compared and estimated. The yield of Lentinula edodes GNA01 was identified to be in the following order: water>70% ethanol>80% methanol, but there was no significant difference between 80% methanol and 70% ethanol extracts. The highest total phenolic content (TPC) and total flavonoid content (TFC) were found in water extract, and TPC of 80% methanol extract was higher than that of 70% ethanol extract and TFC of 70% ethanol extract was higher than that of 80% methanol extract. Water extract exhibited the strongest DPPH, ABTS radicals, and nitrite scavenging activities, $Fe^{2+}$ chelating ability, and FRAP among the three extracts. In addition, antioxidant activity of 80% methanol extract was higher than that of 70% ethanol extract in most of the experiments. As a result, antioxidant activity of Lentinula edodes GNA01 showed a difference according to extraction solvent and concentration; nevertheless, water extract exhibiting high polarity had the strongest antioxidant effect. Consequently, water extract from Lentinula edodes GNA01 is anticipated to be useful for the development of a high value-added functional product.

• 한국식생활문화학회지
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• 제31권5호
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• pp.481-488
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• 2016

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of Eugenia caryophyllata Thunb. water and 70% ethanol extracts. The content of total polyphenol was significantly higher in water extract than in 70% ethanol extract. The DPPH radical scavenging activity of water extract was similar to that of Vit. C at a concentration of $1,000{\mu}g/mL$. The ABTS radical scavenging activities of water and 70% ethanol extract were similar to that of Vit. C at a concentration of $1,000{\mu}g/mL$. SOD-like activity of water extract was higher than that of 70% ethanol extract at a concentration of $1,000{\mu}g/mL$ but lower than that of Vit. C. The DPPH radical scavenging activity, ABTS radical scavenging activity, and SOD-like activity increased as concentrations of water and 70% ethanol extracts increased. Cell cytotoxicity was not observed at all concentrations except at $100{\mu}g/mL$ concentration of water extract. Inhibitory activity on NO production effect of water extract was significantly higher than that of 70% ethanol extract. These results show that E. caryophyllata Thunb. has potent biological activities, and their activities were different depending on extraction solvent.

• 한국식품영양학회지
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• 제13권2호
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• pp.152-157
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• 2000

In order to select the optimum drying method for the production of cinnamon extract, water extract and 70% ethanol extract of cinnamon were prepared. And then several drying method of oven drying, vacuum evaporation, spray drying and freeze drying were performed. Content of cinnamic acid, cinnamic aldehyde, eugenol, tannin and free sugar, and antioxidant activity, degree of browning, pH, color value, turbity and solubility were compared. In water extract, contents of cinnamic acid, cinnamic aldehyde, eugenol were 29.45mg/100g, 94.86mg/100g, 120.75mg/100g and decreased to 4.76%∼44.21%, 5.30%∼48.05%, 3.66%∼21.83% by oven dyring, vaccum drying, spray drying respectively, but freeze drying showed a little decrease of those components. In 70% ethanol extract, effectual components decreased to 76.05%∼88.38% and 26.86%∼78.76% by freeze drying and vacuum evaporation respectively. Antioxidant activity decreased by drying and decreasing rate in 70% ethanol extract was lower than water extract. Degree of browning increased as the drying temperature increased. Tannin and free sugars were little affected by drying temperature. Solubility decreased in oven drying and 70% ethanol extract. Overall data suggested that optimum drying methods of cinnamon extract were freeze drying in case of water extract and freeze drying and vaccum drying in case of 70% ethanol extract.

• 대한본초학회지
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• 제31권6호
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• pp.45-52
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• 2016

Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were $24.6mg/m{\ell}$ and $14.3mg/m{\ell}$, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.

• 한국식품영양학회지
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• 제33권2호
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• pp.210-214
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• 2020

In this study, polyphenol and flavonoid contents were measured, and DPPH, OH, H₂O₂ radical scavenging activity, and the α-amylase inhibitory activity were measured to study the antioxidant activity of 70% ethanol extract from Morinda citrifolia L. The polyphenol and flavonoid contents of noni 70% ethanol extract were 29.52 GAE/g and 12.48 CE/g, respectively. Also, the IC₅₀ values of DPPH, hydroxyl radical, and hydrogen peroxide scavenging activity of 70% ethanol extract from noni were 18.70 mg/mL, 26.45 mg/mL, and 35.67 mg/mL, respectively. Measurement of the α-amylase inhibitory activity of 70% ethanol extract from noni showed 45% inhibitory activity at 10 mg/mL.

• 한국산학기술학회논문지
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• 제14권10호
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• pp.5091-5096
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• 2013

본 연구는 여러 생리활성 성분이 다량 함유되어 있다고 알려진 전통 약용 식물인 어성초의 추출 조건에 따른 항산화력을 관찰하였는데, 추출조건으로는 식용으로 활용가능한 물 추출과 70% 에탄올 추출을 통해 항산화력을 비교하였다. 항산화력은 총 페놀 함량, DPPH 라디칼 소거능, ABS 양이온 소거능, FRAP 방법으로 측정하였다. 결과적으로 총 페놀 함량은 어성초 물추출물이나 70% 에탄올 추출물 모두 77.8 mM Tannic acid/g extract로 나타나 유의적인 차이는 없었고 DPPH 라디칼 소거능은 물 추출물 2 mg/mL 농도에서 78.4%, 70% 에탄올 추출물은 91.8%로 유의적으로 소거능이 70% 에탄올 추출 시에 우수한 것으로 나타났다. 또한 ABTS 양이온 소거능에서도 물 추출물은 2 mg/mL 농도에서 47.7%, 70% 에탄올 추출물에서는 54.8%로 유의적으로 70% 에탄올 추출물이 저해능이 우수한 것으로 나타났으며 FRAP 방법으로 통한 환원 능도 70% 에탄올 추출물이 우수한 것으로 관찰되었다. 이와 같은 결과로 어성초 추출물 조건중 70% 에탄올 추출 시 항산화력을 높힐 수 있는 생리활성 성분이 추출되는 것으로 사료되며 식용 가능한 기능성 식품소재로써 어성초 에탄올 추출물을 사용할 수 있을 것으로 사료된다.

• 생명과학회지
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• 제27권1호
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• pp.23-31
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• 2017

본 연구에서는 전처리 조건(음건, 숙성, 덖음)과 추출 용매 중 에탄올의 혼합비율(30, 50, 70, 90%)에 따른 섬애약쑥 추출물의 이화학적 특성과 항산화 활성을 비교하여, 최적의 전처리 조건 및 추출용매를 확인하고자 하였다. 추출물의 수율은 음건쑥추출물에 비해 숙성쑥과 덖음쑥 추출물이 더 높았고, 에탄올 농도에 따른 경향은 확인되지 않았다. 색도는 L, a, b값 모두 음건쑥 추출물에서 가장 높았고 열처리 시간이 가장 길었던 숙성쑥 추출물에서 유의적으로 낮았다. 가용성 고형분 함량은 덖음쑥, 숙성쑥, 건조쑥 순으로 높고 에탄올의 비율이 증가할수록 그 함량 또한 증가하였다. 총 페놀, jaceosidin, eupatilin 및 총 플라보노이드 함량 또한 덖음쑥, 숙성쑥, 건조쑥 순으로 높았으며, 전반적으로 50%와 70% 에탄올 추출물이 30%와 95% 에탄올 추출물에 비해 유의적으로 높은 함량이었다. DPPH radical 소거 활성과 ABTS 라디칼 소거활성은 덖음쑥 추출물이 음건쑥과 숙성쑥 추출물에 비해 높았는데, DPPH radical 소거 활성은 덖음쑥 70% 에탄올 추출물이 77.71%로 유의하게 가장 높았으며, ABTS radical 소거 활성은 덖음쑥 30%, 50% 및 70% 에탄올 추출물이 96% 이상의 높은 활성을 나타내었다. 이상의 결과로부터 섬애약쑥은 음건한 것을 덖음 처리를 한 후 50%~70% 에탄올로 추출할 때 유효성분의 함량과 라디칼 소거활성이 높음을 확인하였다.

• 한국조리학회지
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• 제22권6호
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• pp.14-23
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• 2016

This purpose of this study is to examine the antioxidant and antimicrobial activities of Persicaria hydropiper L. extract in 70% ethanol and in water, a medicinal herb, as an effort to examine the potential of medicinal herbs for development of antioxidants and natural preservative substitutes. The total poly-phenol content in the 70% ethanol extract and in the water extract were 19.88 mg/g and 7.46 mg/g, respectively. The DPPH radical scavenging activity was 90.99% and 64.98% in the 70% ethanol extract and water extract, in which 70% ethanol extract showed a higher activity. The antioxidant effect of Persicaria hydropiper L. extract appears to be very good and due to its excellent growth inhibitory effect on food-poisoning-causing microorganisms in the food, it is thought to be utilized as a potential natural preservative substitute in many areas.