• Applied Chemistry for Engineering
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• v.32 no.4
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• pp.393-402
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• 2021

In this study, a mixed matrix membrane was prepared by putting the zeolitic imidazolate framework-7 (ZIF-7) synthesized in Pebax-1657 and Pebax-2533, which are representative poly(ether-b-amide), and the permeability properties of single gas such as N₂ and CO₂ were investigated. From the gas permeation results, in the case of N₂, both the Pebax-1657/ZIF-7 and Pebax-2533/ZIF-7 mixed matrix membranes showed a similar phenomenon in which the permeability decreased with the incorporation of ZIF-7. For CO₂ permeability, the tendency was slightly different depending on the type of polymer. In the Pebax-1657/ZIF-7 mixed membrane, the CO₂ permeability decreased in the range of 0~3 wt% of ZIF-7, and increased at higher contents. The CO₂ permeability of the Pebax-2533/ZIF-7 mixed matrix membrane gradually decreased without increasing the permeability in the range of 0~5 wt% of ZIF-7. Regarding CO₂/N₂ selectivity, both mixed films showed a tendency to increase with increasing the ZIF-7 content. In particular, Pebax-2533/ZIF-7 5 wt% showed the best gas permeation performance compared to other mixed matrix membrane. This is thought to be because ZIF-7 shows better compatibility with Pebax-2533 than that of Pebax-1657 and also better CO₂ selective property.

• Journal of Microbiology and Biotechnology
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• v.6 no.5
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• pp.336-339
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• 1996

Glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. SY-77-1 was immobilized with oxiran acrylic beads for the production of 7-aminocephalosporanic acid (7-ACA) from glutaryl 7-aminocephalosporanic acid (GL 7-ACA). The immobilized enzyme maintained its activity at a constant level for 7 days, but lost 30$%$ of its activity after 20 days. Optimal reaction conditions for the synthesis of 7-ACA were found to be $30^{\circ}C$ and pH 8.0 using the immobilized enzyme. For the economic production of 7-ACA, substrate and enzyme concentrations were optimized to 60 mM and 0.5 g wet weight per 10 $m\ell$ of reaction volume, respectively. Under optimized conditions, 50 mM 7-ACA was produced from 60mM GL 7-ACA within 8 h, resulting in a conversion yield of 83$%$.

• Journal of Korean Ophthalmic Optics Society
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• v.1 no.2
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• pp.71-76
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• 1996

As the results of measurement of the radius of corneal curvature with keratometer, for the right eye of 91 male adults, horizontal radius is 7.16 mm to 8.49 mm and vertical radius is 7.03 mm to 8.34 mm, and for the left eye of male adults, horizontal radius is 7.10 mm to 8.48 mm and vertical radius is 7.01 mm to 8.24 mm. In case of 61 female adults, horizontal radius is 7.16 mm to 8.45 mm, vertical radius 7.11 mm to 8.18 mm for the right eye, and horizontal radius 7.15 mm 108.43 mm and vertical radius 7.01 mm to 8.26 mm for the left eye. The mean value of radius of corneal curvature is 7.74 mm for male and 7.69 mm for female. Also the mean value of horizontal radius is 7.79 mm and vertical radius is 7.64 mm. Overall mean value of the radius of corneal curvature is 7.71 mm that is corresponded to Gullstrand's eye. The horizontal radius is 0.15 mm bigger than vertical radius.

• Korean Journal of Poultry Science
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• v.46 no.3
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• pp.173-183
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• 2019

Based on their virulence, the avian influenza viruses (AIVs) are classified into two pathotypes: low pathogenic avian influenza (LPAI) virus and highly pathogenic avian influenza (HPAI) virus. Among the 16 HA subtypes of AIV, only the H5 and H7 subtypes are classified as HPAI. Some AIVs, including H5 and H7 viruses, can infect humans directly. Six H7 subtype isolates from wild birds of the H7N7 (n=4) and H7N1 (n=2) subtypes were characterized in this study. Phylogenetic analysis showed that eight viral genes (HA, NA, PB2, PB1, PA, NP, M, and NS) of the H7 isolates clustered in the Eurasian lineage, the genetic diversity of which is indicated by its division into several sublineages. The Korean H7 isolates had two motifs, PEIPKGR and PELPKGR, at the HA cleavage site, which have been associated with LPAI viruses. Six H7 isolates encoded glutamine (Q) and glycine (G) at positions 226 (H3 numbering) and 228 of HA, suggesting avian-type receptor-binding specificity. None of the Korean H7 isolates had the amino acid substitutions E627K in PB2 and I368V in PB1, which are critical for efficient replication in human cells. The Korean H7 isolates showed no deletions in the NA stalk region and in NS. These results suggest that the Korean H7 isolates from wild birds are different from the H7N9 influenza viruses isolated in China in 2013, which are capable of infecting humans.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.667-667
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• 2000

Pseudomonas sp. BK7, an alkalophile, displayed the highest growth and protease activity when grown in a fermenter which was controlled at a pH level of 9.0, and the enzyme production was significantly enganced by the increase of agitation speed. Two formas of alkaline proteases (BK7-1 and BK7-2) were fractionated and purified to near homogeneity. Protease BK7-1 was purified through CM-Sepharose CL-6B and Sephadex G-75 column chromatographies, and Protease BK7-2 was purified through CM-Sepharose CL-6B and Sephadex G-75 column chromatographies, and Protease BK7-2 was purified through CM-Sepharose CL-6B, DEAE-Sepharose, and Sephadex G-75 column chromatographies. The molecular weights of proteases BK7-1 and BK7-2 determined by gel filtration chromatography were 20,700 and 40,800, respectively. The $K_m$ value, isoelectric point, and optimum pH of protease BK7-1 were 2.55 mg/ml, 11.0 and 11.0, respectively, whereas those of protease BK7-2 were 1.57 mg/ml, 7.2, and 10.0, respectively. Both protease were practically stable in the pH range of 5-11. The optimum temperatures for the activities of both protease BK7-1 and BK7-2 were 50℃ and 45℃, respectively. About 56% of the original protease BK7-2 activity remained after being treated at 50℃ for 30 min but protease BK7-1 was rapidly inactivated at above 25℃. Both proteases were completely inhibited by phenylmethane sulfonyl fluoride, a serine protease inhibitor. Protease BK7-2 was stable against EDTA, EGTA, STP, and detergents such as SDS and LAS, whereas protease BK7-1 was found to be unstable.

• Annual Conference on Human and Language Technology
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• 2018.10a
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• pp.520-521
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• 2018

의료정보 교환 국제 표준인 HL7의 처리를 위한 인터페이스 소프트웨어에서 다양한 버전 사이의 호환성 문제의 해결 방법으로 객체 지향적 구조에 기반한 HL7 파서(Parser)의 구조가 설계되었다. 그러나 수천개의 HL7 메시지 및 구성요소를 처리할 수 있는 HL7 파서를 직접 구현하기 위해서 많은 시간과 인력이 필요하다. 또한, 구현된 파서의 기능 변경 시 수천 개의 파일을 프로그래머가 직접 수정하는 방법은 매우 비효율적이다. 따라서 본 연구에서는 버전 상호 호환 가능한 HL7 파서의 설계에 따라 HL7 파서를 효과적인 구현할 수 있는 방법을 제안한다. 제안한 방법에 따라 HL7 파서를 구현하고, HL7 메시지의 버전 간 변환 테스트를 수행하여 개발한 파서의 효용성을 확인한다.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2001.04a
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• pp.41-50
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• 2001

한반도 주요 지체구조구에 대한 최대지진을 지진 및 지질자료를 이용하여 여러 가지 방법으로 결정하였다. 한반도에서 발생한 가장 큰 지진은 MMI IX - X의 범위에 걸치며 이는 M= 7.0 - 7.7 에 해당한다. Gumbel의 극대치 제3분포를 이용하면 지체구조구별 최대지진은 M = 7.1 - 7.9의 범위에 놓이고, 응력방출 양상을 분석하면 M = 6.7 - 7.7 가 도출된다. 단층길이와 규모와의 상관관계에서 최대지진은 M = 7.4 - 7.6 에 놓인다. 한반도의 주요 지체구조구 사이에 최대지진의 현격한 차이를 나타내는 지진 및 지질학적 증거는 없다. 역사지진의 평가에서 강진들은 대략 1 계급( M=0.7) 과대 평가되는 경향이 있으므로, 한반도의 최대지진은 대략 M = 7.0으로 추정된다.

• YAKHAK HOEJI
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• v.23 no.1
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• pp.51-55
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• 1979

"Ha-Neul-Ta-Ri" (Trichosanthes kirilowii Max., Cucurbitaceae) cultivated in Korea and used in popular medicine, was investigated for sterol composition of the seed oil. This seed oil contains unusual sterols having ${\delta}^{7, 22}_-$, ${\delta}^{7, 24}_-$ and ${\delta}^{7, 22, 25}_-$ di and trienols. ${\delta}^{7, 22}_-$ Stigmastadienol and ${\delta}^{7, 22, 25}_-$ Stigmastatrienol were identified as the major sterols in the seed oil of Trichosanthes kirilowii by gas loiquid chromatographic analysis. The results showed that contents of the seed sterols were campesterol 1.0-1.2%, stigmasterol 1.5-1.7%, ${\delta}^{7}_-$ campstenol 0.6-0.8%, sitosterol 10.6-11.4%, ${\delta}^{7, 22}_-$ stigmastadienol 39.1-41.8%, ${\delta}^{7, 22, 25}_-$ stigmastatrienol 28.9-29.3%, ${\delta}^{7, 25}_-$ stigmastadienol 14.9-15.5% and ${\delta}^{7, 24}_-$ stigmastadienol 0.7-1.0%.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.04b
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• pp.51-56
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• 2001

This study was carried out to research the effect of nutrient composition and GA4＋7 on growth, flower quality and bulb development in special hydroponics of cut tulip. Flowering of 'Cassini' was markedly accelerated by standard+GA4＋7, K200＋GA4＋7, N250＋GA4＋7 treatment, last internode was increased by N250 as compared with other treatment. For 'Christmas Marvel', flowering was promoted by K250＋GA4＋7, last infernode and total length were also increased with K250＋GA4＋7 as compared to other treatment. For 'Golden Apeldoorn', flowering was accelerated by K200＋GA4＋7, last internode was increased with N300＋GA4＋7 treatment and total length was increased in N250＋GA4＋7 treatment as compared with the standard plants. Bulb weight and number of 'Cassini', 'Christmas Marvel'and 'Golden Apeldoorn' were decreased by GA4＋7 as compared to non-treatment, and bulb diameter was not affected by GA4＋7.

• Development and Reproduction
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• v.26 no.3
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• pp.117-126
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• 2022

Bromodomain-containing protein 7 (BRD7) participates in many cellular processes and embryo development. BRD7 is down-regulated in various cancers and evidence of its tumor suppressor function has been accumulating. Here, we identified transforming stimulated clone 22 (TSC-22) as a novel BRD7 interacting protein and show its novel function as a positive regulator of BRD7. We found that TSC-22 expression potentiated the inactivation of the extracellular signal-regulate kinase (ERK) pathway by BRD7. Our data establishes TSC-22 as a modulator of BRD7 and unravels the molecular mechanisms that drive the synergistic tumor-suppressing effects of TSC-22 and BRD7. Our findings may open new avenues for developing novel molecular therapies for tumors exhibiting down-regulated BRD7 and/or TSC-22.