• 한국자동차공학회논문집
• /
• 제2권6호
• /
• pp.94-101
• /
• 1994

About vibration model of Six-degrees-of-freedom(DOF), in mass load, examined results for knowing dynamic interference and response variation is as follows; In case of putting mass load upon the object, experimented results on two-degrees-of-freedom of the translation-1 direction and the rotation-1 direction at open-loop-control system, about 0.19 arcsed in input of the translation-$0.1{\mu}m$ and $0.022{\mu}m$ on input of the rotation-0.5 arcsec, the justicse of motion equation is acknowledged as confirming the appearance of the interference-$0.022{\mu}m$. In establishing calculation of transformation matrix by using analogue circuit, as simulating results that used incomplete differentiation, interference is $1.7{\times}10^{-3}$ arcsec on input of the translation-$0.1{\mu}m$ and $1.4{\times}10^{4}{\mu}m$ on input of the rotation-0.5 arcsec in open-loop-control system. Also it is $4.2{\times}10^{-4}$ arcsec on input of the translation-$0.1{\mu}m$ and $5.6{\times}10^{-5}{\mu}m$ on input of the rotation-0.5 arcesc in closed-loop-control system. As closed-loop-control system is better than open-loop-control system, equivalent accordance is confirmed on original response. Finally, fundamental validity of this theory is acknowledged.

• 대한한의학회지
• /
• 제24권3호
• /
• pp.145-154
• /
• 2003

Background : Production of cytokines by bronchial epithelial cells may contribute to the local accumulation of inflammatory cells in patients with bronchial asthma. In many recent studies, molecular biological methods have been used to investigate the role of cytokines in pathogenesis and new therapeutic targets of asthma. Objective : We aimed to identify the dose-dependent inhibitory effects of Lonicerae Flos and Paeoniae Radix on the mRNA expression of IL-6, IL-16, and GM-CSF involved in the asthma model. Materials and Methods : In the study BEAS-2B cell lines, human epithelial cells were used. These cells were stimulated with tumor necrosis factor $(TNF)-\alpha$ for artificial inflammatory expression. ${\beta}-actin$ messenger RNA (mRNA) was used for internal standard. After 24 hours of Lonicerae Flos, Paeoniae Radix, total cellular RNAs were collected treating RNA zol directly on the living cells. Then the transcriptional activities of IL-6, 16, GM-CSF were measured by RT- PCR with electrophoresis. Results : In the Lonicerae Flos study, the mRNA expression of IL-6, IL-16 and GM-CSF was showed no inhibitory effect compared to the control group in all concentrations. In the Paeoniae Radix study, the mRNA expression of IL-6, IL-l6 and GM-CSF was showed no inhibitory effect compared to the control group in all concentrations. Conclusion : This study shows that Lonicerae Flus and Paeuniae Radix have no inhibitory effects on the mRNA expression of IL-6, IL-16 and GM-CSF in BEAS-2B cell lines, human epithelial cells. Advanced studies are required to investigate the other mechanisms of inhibitory effect by Lonicerae Flus and Paeoniae Radix in the asthma model.

• 대한한의학회지
• /
• 제23권1호
• /
• pp.11-23
• /
• 2002

Objectives: We aimed to identify the dose-dependent inhibitory effects of Maekmundongcheongpye-eum and Liriopis Tuber on the mRNA expression of IL-6, IL-16, GM-CSF involved in the asthma model. Methods: In the study BEAS-2B cell lines, human epithelial cells were used. These cells were stimulated with tumor necrosis factor $(TNF)-{\alpha}$ for artificial inflammatory expression. ${\beta}-actin$ messenger RNA (mRNA) was used by internal standard. After 24 hours of Maekmundongcheongpye-eum, Liriopis Tuber-treatment, total cellular RNAs were collected, treating RNAzol directly on the alive cells. Then the transcriptional activities of IL-6, 16, GM-CSF were measured by RT-PCR with electrophoresis. Results: In the Maekmundongcheongpye-eum study, the mRNA expression of IL-6 showed 48% transcriptional inhibitory effect compared to the control group in the $100{\;}{\mu}l/ml$ category (P<0.001). In the IL-16, there was 53% and 57% transcriptional inhibitory effect compared to the control group in the $20{\;}{\mu}l/ml$ and $100{\;}{\mu}l/ml$ categories (P<0.001). In the GM-CSF, there was no inhibitory effect. In the Liriopis Tuber study, the mRNA expression of IL-6 showed 43% transcriptional inhibitory effect compared to the control group in the $100{\;}{\mu}l/ml$ category (p<0.005). In the IL-16, 34% and 26% of transcriptional inhibitory effect was shown compared to the control group in the $20{\;}{\mu}l/ml$ and $100{\;}{\mu}l/ml$ categories, respectively (P<0.05). In the GM-CSF, there was no inhibitory effect. Conclusions: This study shows that Maekmundongcheongpye-eum and Liriopis Tuber have dose-dependent inhibitory effects on the mRNA expression of IL-6 and IL-16 in BEAS-2B cell lines, human epithelial cells. Advanced studies are required to investigate the mechanisms of inhibition by herbal medicine in the asthma model.

• 한국식품과학회지
• /
• 제22권2호
• /
• pp.177-182
• /
• 1990

역류분배방법을 이용한 단백질의 분리정제 효율을 높이기 위하여, 4가지 모델단백질에 대하여 수용성액체 2상계에서의 분획계수를 KCl 농도와 pH변화에 따라 조사하였다. 또한, 역류분배방법을 이용한 다중분배 추출방식으로 모델단백질의 혼합물을 KCl 농도(0, 50, 250, 500mM)와 pH(4.5, 5.5, 6.5, 9.0, 12.0)를 달리한 20가지 조건에서 분획하였다. 그 결과 KCl 농도와 pH에 따라 단백질들의 분획계수(K)값이 다르게 나타나서, KCl을 첨가함으로서 역류분배방법을 이용한 단백질 분리효율을 향상시킬 수 있는 가능성을 보여 주었다. 단백질에 따라 분리가 잘 일어나는 KCl 농도와 pH는 다르게 나타나서 myoglobin의 경우 KCl 50mM, pH5.5조건의 14번 튜브 상층부와 KCl 250mM, pH6.5조건의 13번 튜브의 상층부에서 잘 분리가 되었으며, BSA의 경우는 KCl 250mM, pH4.5 조건의 14번 튜브에서 분리되었고, lysozyme의 경우는 KCl 50MM, pH4.5조건의 19번 튜브와 KCl 50mM, pH5.5의 16번 튜브 상층부에서 분리가 되었다.

• 한국지반공학회논문집
• /
• 제34권4호
• /
• pp.49-55
• /
• 2018

근접굴착 시 지반변위를 억제하여 기존건물의 안정성을 확보하기 위해 굴착공사 중 흙막이벽체의 버팀대에 선행하중을 가하고 그 효과를 확인하였다. 선행하중을 가한 경우와 가하지 않은 경우에 대해서도 대형모형실험을 하였다. 지표면에 건물하중이 없는 경우와 건물하중이 있는 경우 각각 0m, 1D, 2D 에 대해 대형모형실험을 실시하였다. 본 연구에서는 모형실험에는 12m 폭의 기존건물을 축척 1:10으로 사용하였고, 폭 2m, 높이 6m, 길이 4m를 가진 대형토조에서 흙막이벽체와 인접건물의 거동에 대하여 실험을 수행하였다. 그 결과, 흙막이벽체의 버팀대에 선행하중을 작용시켜 흙막이벽체의 수평변위를 억제시켰을때 벽체배면 가상주동활동영역 내에 있는 인접건물의 안정성이 크게 향상되는 것을 확인하였다.

• 한국환경과학회지
• /
• 제7권6호
• /
• pp.833-843
• /
• 1998

It is noted that the red tides and the oxygen-deficient water mass are extensively developed in Masan Bay during summer. The nutrients mass balance was calculated in Masan Bay, using the three-dimensional numerical hydrodynamic model and the material cycle model. The material cycle model was calibrated with the data obtained on the field of the study area in June 1993. The nutrients mass balance calculated by the combination of the residual currents and material cycle model results showed nutrients of surface and middle levels to be transported from the inner part to the outer part of Masan Bay, and nutrients of bottom level to be transported from outer part to inner part of Masan Bay. The uptake rate of DIN in the box A1(surface level of inner part) was found to be 337. 5mg/$m^3$ㆍday, the largest value in all 9 boxes and that of DIP was found to be 18.6mg/$m^3$ㆍday in box A1, and the regeneration rate of DIN was found to be 78.2mg/$m^3$ㆍday in the box A3(bottom level of inner part), and that of DIP was found to be 18.6mg/$m^3$ㆍday in box A1. The regenerations of DIN and DIP in the water column of the entire Bay were found to be 7.66ton/day and 760kg/day, respectively. And the releases of DIN and DIP from the sediments of the entire Bay were found to be 2.86ton/day and 634kg/day, respectively. The regeneration rate was 2.5 times as high as the release rate in DIN, and 1.2 times in DIP. The results of mass balance calculation showed not only the nutrients released from the sediments but the nutrients regenerated in water column to be important in the control and management of water quality in Masan Bay.

• Geomechanics and Engineering
• /
• 제36권5호
• /
• pp.427-440
• /
• 2024

This study concentrates on the 301 comprehensive caving working face, notable for its considerable mining height. The roof model is established by integrating prior geological data and the latest borehole rock stratum's physical and mechanical parameters. This comprehensive approach enables the determination of lithology, thickness, and mechanical properties of the roof within 50 m of the primary mining coal seam. Utilizing the transfer rock beam theory and incorporating mining pressure monitoring data, the study delves into the geometric parameters of the direct roof, basic roof movement, and roof pressure during the initial mining process of the 301 comprehensive caving working face. The direct roof of the mining working face is stratified into upper and lower sections. The lower direct roof consists of 6.0 m thick coarse sandstone, while the upper direct roof comprises 9.2 m coarse sandstone, 2.6 m sandy mudstone, and 2.8 m medium sandstone. The basic roof stratum, totaling 22.1 m in thickness, includes layers such as silty sand, medium sandstone, sandy mudstone, and coal. The first pressure step of the basic roof is 61.6 m, with theoretical research indicating a maximum roof pressure of 1.62 MPa during periodic pressure. Extensive simulations and analyses of roof subsidence and advanced abutment pressure under varying working face lengths. Optimal roof control effect is observed when the mining face length falls within the range of 140 m-155 m. This study holds significance as it optimizes the working face length in thick coal seams, enhancing safety and efficiency in coal mining operations.

• 한국자기학회지
• /
• 제21권1호
• /
• pp.15-22
• /
• 2011

강자성체를 정밀해석하기 위해서는 포화자화 현상과 히스테리시스 현상을 모두 고려해 주어야만 한다. Preisach 모델링은 변화율이 심한 히스테리시스 현상을 매우 잘 표현해 낼 수 있는 수치모사 방법이다. 하지만 정자기 유한요소법과 Preisach 모델링을 사용한 자화해석 반복 과정에서 수렴성이 떨어진다는 큰 문제점을 가지고 있다. 특히 자화율의 기울기가 급격히 변화할 경우 수렴에 많은 문제점을 가지고 있다. 이러한 문제점은 일반적인 M-H 변수를 사용하지 않고, M-B 변수를 사용하면 해결할 수 있다. 본 논문에서는 일반적인 M-H를 이용한 Preisach 모델링의 평면 분포로부터 M-H 변수를 M-B 변수화 시키는 방법을 제안하고 있고, 2차원 정자기 유한요소법과 M-H, M-B 변수를 이용한 Preisach 모델링을 비교 분석하여 Preisach 모델링의 수렴성 문제를 해결하는 방법에 대해 기술하였다.

• Journal of Power Electronics
• /
• 제10권6호
• /
• pp.739-748
• /
• 2010

The increased integration of fuel cells with power electronics, critical loads, and control systems has prompted recent interest in accurate electrical terminal models of the polymer electrolyte membrane (PEM) fuel cell. Advancement in computing technologies, particularly parallel computation techniques and various real-time simulation tools have allowed the prototyping of novel apparatus to be investigated in a virtual system under a wide range of realistic conditions repeatedly, safely, and economically. This paper builds upon both advancements and provides a means of optimized model construction boosting computation speeds for a fuel cell model on a real-time simulator which can be used in a power hardware-in-the-loop (PHIL) application. Significant improvement in computation time has been achieved. The effectiveness of the proposed model developed on Opal RT's RT-Lab Matlab/Simulink based real-time engineering simulator is verified using experimental results from a Ballard Nexa fuel cell system.

• Bulletin of the Korean Chemical Society
• /
• 제29권6호
• /
• pp.1137-1140
• /
• 2008

M1 RNA, the catalytic subunit of Escherichia coli RNase P, is an essential ribozyme that processes the 5' leader sequence of tRNA precursors (ptRNAs). Using KS2003, an E. coli strain generating only low levels of M1 RNA, which showed growth defects, we examined whether M1 RNA is involved in polycistronic mRNA processing or degradation. Microarray analysis of total RNA from KS2003 revealed six polycistronic operon mRNAs (acpP-fabF, cysDNC, flgAMN, lepAB, phoPQ, and puuCBE) showing large differences in expression between the adjacent genes in the same mRNA transcript compared with the KS2001 wild type strain. Model substrates spanning an adjacent pair of genes for each polycistronic mRNA were tested for RNase P cleavage in vitro. Five model RNAs (cysNC, flgMN, lepAB, phoPQ, and puuBE) were cleaved by RNase P holoenzyme but not by M1 RNA alone. However, the cleavages occurred at non-ptRNA-like cleavage sites, with much less efficiency than the cleavage of ptRNA. Since cleavage products generated by RNase P from a polycistronic mRNA can have different in vivo stabilities, our results suggest that RNase P cleavage may lead to differential expression of each cistron.