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Construction of Genetic Microsatellite Maps for Some Chromosomes in Chinese Swine Reference Population

  • Su, Yuhong;Xiong, Yuanzhu;Zhang, Qin;Liu, Weimin;Jiang, Siwen;Yu, Li;Xia, Xuanyan;Zeng, Rong;Deng, Changyan
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.10
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    • pp.1386-1390
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    • 2002
  • In aiming to identify the genes or genetic regions responsible for quantitative traits, a swine reference population had been constructed using three Large White boars and seven Meishan dams as parents. Five $F_1$ males and 23 $F_1$ females were intercrossed to generate 147 $F_2$ offspring. Thirty-one microsatellite markers covering Sus scrofa chromosomes (SSC) 2, 4, 6 and 7 were genotyped for all members. Construction of genetic microsatellite maps was performed using the CRIMAP software package. The lengths of these chromosomes were longer than MARC maps. They were 158.6cM, 180.3cM, 197.3cM and 171.4cM, respectively. A two modified orders of markers were observed for SSC6 and SSC7. The female map on SSC6 was shorter than male map, and the contrary was on SSC 2, 4 and 7.

Improved Electron Injection on Organic Light-emitting Diodes with an Organic Electron Injection Layer

  • Kim, Jun-Ho;Suh, Chung-Ha;Kwak, Mi-Young;Kim, Bong-Ok;Kim, Young-Kwan
    • Transactions on Electrical and Electronic Materials
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    • v.6 no.5
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    • pp.221-224
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    • 2005
  • To overcome of poor electron injection in organic light-emitting diodes (OLEDs) with Al cathode, a thin layer of inorganic insulating materials, like as LiF, is inserted between an Al cathode and an organic electron transport layer. Though the device, mentioned above, improves both turn on voltage and luminescent properties, it has some problems like as thickness restriction, less than 2 nm, and difficulty of deposition control. On the other hand, Li organic complex, Liq, is less thickness restrictive and easy to deposit and it also enhances the performance of devices. This paper reports the improved electron injection on OLEDs with another I A group metal complex, Potassium quinolate (Kq), as an electron injection material. OLEDs with organic complexes showed improved turn-on voltage and luminous efficiency which are remarkably improved compared to OLEDs with Al cathode. Especially, OLEDs with Kq have longer life time than OLEDs with Liq.

Comparison of Intensity Modulated Radiation Therapy Dose Calculations with a PBC and AAA Algorithms in the Lung Cancer (폐암의 세기조절방사선치료에서 PBC 알고리즘과 AAA 알고리즘의 비교연구)

  • Oh, Se-An;Kang, Min-Kyu;Yea, Ji-Woon;Kim, Sung-Hoon;Kim, Ki-Hwan;Kim, Sung-Kyu
    • Progress in Medical Physics
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    • v.23 no.1
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    • pp.48-53
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    • 2012
  • The pencil beam convolution (PBC) algorithms in radiation treatment planning system have been widely used to calculate the radiation dose. A new photon dose calculation algorithm, referred to as the anisotropic analytical algorithm (AAA), was released for use by the Varian medical system. The aim of this paper was to investigate the difference in dose calculation between the AAA and PBC algorithm using the intensity modulated radiation therapy (IMRT) plan for lung cancer cases that were inhomogeneous in the low density. We quantitatively analyzed the differences in dose using the eclipse planning system (Varian Medical System, Palo Alto, CA) and I'mRT matirxx (IBA, Schwarzenbruck, Germany) equipment to compare the gamma evaluation. 11 patients with lung cancer at various sites were used in this study. We also used the TLD-100 (LiF) to measure the differences in dose between the calculated dose and measured dose in the Alderson Rando phantom. The maximum, mean, minimum dose for the normal tissue did not change significantly. But the volume of the PTV covered by the 95% isodose curve was decreased by 6% in the lung due to the difference in the algorithms. The difference dose between the calculated dose by the PBC algorithms and AAA algorithms and the measured dose with TLD-100 (LiF) in the Alderson Rando phantom was -4.6% and -2.7% respectively. Based on the results of this study, the treatment plan calculated using the AAA algorithms is more accurate in lung sites with a low density when compared to the treatment plan calculated using the PBC algorithms.

Characterization of quercetin and its glycoside derivatives in Malus germplasm

  • Zhang, Lei;Xu, Qipeng;You, Yaohua;Chen, Weifeng;Xiao, Zhengcao;Li, Pengmin;Ma, Fengwang
    • Horticulture, Environment, and Biotechnology : HEB
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    • v.59 no.6
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    • pp.909-917
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    • 2018
  • Quercetin and its glycoside derivatives were identified and quantified using high-performance liquid chromatograph (HPLC) and liquid chromatograph/mass spectrometer/mass spectrometer (LC/MS/MS) in the leaves, flowers, and fruits of 22 Malus genotypes. In all genotypes, small amounts of quercetin aglycone were present, with water-soluble glycoside forms were the most abundant in different Malus plant tissues, including quercetin-3-galactoside, quercetin-3-rutinoside, quercetin-3-glucoside, quercetin-3-xyloside, quercetin-3-arabinoside, and quercetin-3-rhamnoside. Among these six quercetin glycosides, quercetin-3-galactoside was the common form in Malus plants, except in the leaves and flowers of M. ceracifolia and M. magdeburgensis, and in the fruits of M. micromalus 'Haihong Fruit', where there was a higher concentration of quercetin3-glucoside. Among the different tissues tested, leaves contained the highest concentration of quercetin and its glycosides, while fruits contained the lowest concentrations of these compounds. Among the genotypes we analyzed, no specific genotype consistently contained the highest concentration of quercetin and its glycoside derivatives. M. domestica 'Honeycrisp' had the highest total compound concentration (approximately $1600mg\;kg^{-1}$), whereas M. hupehensis contained the lowest in its fruits. In contrast, the concentration of total quercetin and its glycosides was more than $5000mg\;kg^{-1}$ in the leaves of eight genotypes and greater than $2500mg\;kg^{-1}$ in the flowers of seven species. In general, the concentration of quercetin and its glycoside derivatives depended on the species and tissue type. These results may provide useful information for the evaluation and selection of edible Malus fruits and the materials for quercetin glycoside extraction.

Characteristics of lightning impulse breakdowns in inhomogeneous $SF_6$ gas gap (불평등전장에서 $SF_6$ 기체의 뇌임펄스 절연파괴특성)

  • Lee, B.H.;Choi, H.S.;Oh, S.K.;Li, F.
    • Proceedings of the KIEE Conference
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    • 2004.07c
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    • pp.1921-1923
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    • 2004
  • V-p characteristics and the discharge luminous characteristics in inhomogeneous $SF_6$ gas gap under the positive and negative lightning impulse voltages are presented. The test gap was composed of the plane-to-plane with a needle-shaped protrusion. The applied voltage and the predischarge current were measured by the electric field sensor and the shunt of 50 ${\Omega}$, respectively. The light emission signals were observed by a photomultiplier tube, and a high-speed camera. In a consequence, the dielectric strengths of $SF_6$ gas gap under positive lightning impulse voltages were independent of the gas pressure. In the presence of the positive polarity, the branches of discharge channel were created and the directions of the discharge paths were random. On the other hands, the discharge paths of the negative polarity were more thicker and brighter.

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The Luminescent Properties of Red OLED Devices Doped with Two Dopants (2원 첨가 적색 OLED 소자의 발광특성)

  • Kim, Kyong-Min;Ju, Sung-Hoo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.20 no.6
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    • pp.531-535
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    • 2007
  • To invest the luminescent characteristics of red light emitting OLED device, a dual dopant system was incorporated into the emitting layer. The multiple layer OLED device structure was $ITO(1500\;{\AA})/HIL(200\;{\AA})/a-NPD(600\;{\AA})/EML(300\;{\AA})/Alq_3(200\;{\AA})/LiF(7\;{\AA})/Al(1800\;{\AA})$. The concentrations of the rubrene dopant were tested at 0 vol.%, 3 vol.%, 6 vol.% and 9 vol.%. The maximum device efficiency and life time were obtained at the rubrene dopant concentration of 6 vol.%. Emission spectrum and color coordinate of devices showed no relationship with rubrene dopant concentration. Experiment results show that rubrene dopant absorbs energy from $Alq_3$ host and transfer it to RD1 dopant acting as an energy intermediate and influencing the device efficiency, finally the red light is emitted from the RD1 dopant.

Empirical Selection of Informative Microsatellite Markers within Co-ancestry Pig Populations Is Required for Improving the Individual Assignment Efficiency

  • Lia, Y.H.;Chu, H.P.;Jiang, Y.N.;Lin, C.Y.;Li, S.H.;Li, K.T.;Weng, G.J.;Cheng, C.C.;Lu, D.J.;Ju, Y.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.5
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    • pp.616-627
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    • 2014
  • The Lanyu is a miniature pig breed indigenous to Lanyu Island, Taiwan. It is distantly related to Asian and European pig breeds. It has been inbred to generate two breeds and crossed with Landrace and Duroc to produce two hybrids for laboratory use. Selecting sets of informative genetic markers to track the genetic qualities of laboratory animals and stud stock is an important function of genetic databases. For more than two decades, Lanyu derived breeds of common ancestry and crossbreeds have been used to examine the effectiveness of genetic marker selection and optimal approaches for individual assignment. In this paper, these pigs and the following breeds: Berkshire, Duroc, Landrace and Yorkshire, Meishan and Taoyuan, TLRI Black Pig No. 1, and Kaohsiung Animal Propagation Station Black pig are studied to build a genetic reference database. Nineteen microsatellite markers (loci) provide information on genetic variation and differentiation among studied breeds. High differentiation index ($F_{ST}$) and Cavalli-Sforza chord distances give genetic differentiation among breeds, including Lanyu's inbred populations. Inbreeding values ($F_{IS}$) show that Lanyu and its derived inbred breeds have significant loss of heterozygosity. Individual assignment testing of 352 animals was done with different numbers of microsatellite markers in this study. The testing assigned 99% of the animals successfully into their correct reference populations based on 9 to 14 markers ranking D-scores, allelic number, expected heterozygosity ($H_E$) or $F_{ST}$, respectively. All miss-assigned individuals came from close lineage Lanyu breeds. To improve individual assignment among close lineage breeds, microsatellite markers selected from Lanyu populations with high polymorphic, heterozygosity, $F_{ST}$ and D-scores were used. Only 6 to 8 markers ranking $H_E$, $F_{ST}$ or allelic number were required to obtain 99% assignment accuracy. This result suggests empirical examination of assignment-error rates is required if discernible levels of co-ancestry exist. In the reference group, optimum assignment accuracy was achievable achieved through a combination of different markers by ranking the heterozygosity, $F_{ST}$ and allelic number of close lineage populations.

Diagnostic Accuracy of 18F-FDG-PET in Patients with Testicular Cancer: a Meta-analysis

  • Zhao, Jing-Yi;Ma, Xue-Lei;Li, Yan-Yan;Zhang, Bing-Lan;Li, Min-Min;Ma, Xue-Lei;Liu, Lei
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.8
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    • pp.3525-3531
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    • 2014
  • Objective: Fluorine-18-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is a new technique for identifying different malignant tumors using different uptake values between tumor cells and normal tissues. Here we assessed the diagnostic accuracy of 18F-FDG-PET in patients with testicular cancer by pooling data of existing trials in a meta-analysis. Methods: PubMed/MEDLINE, Embase and Cochrane Central Trials databases were searched and studies published in English relating to the diagnostic value of FDG-PET for testicular cancer were collected. The summary receiver operating characteristic (SROC) curve was used to examine the FDG-PET accuracy. Results: A total of 16 studies which included 957 examinations in 807 patients (median age, 31.1 years) were analyzed. A meta-analysis was performed to combine the sensitivity and specificity and their 95% confidence intervals (CIs), from diagnostic odds ratio (DOR), positive likelihood ratios (PLR), negative likelihood ratio (NLR). SROC were derived to demonstrate the diagnostic accuracy of FDG-PET for testicular cancer. The pooled sensitivity and specificity were 0.75 (95% confidence interval (CI), 0.70-0.80) and 0.87 (95% CI, 0.84-0.89), respectively. The pooled DOR was 35.6 (95% CI, 12.9-98.3). The area under the curve (AUC) was 0.88. The pooled PLR and pooled NLR were 7.80 (95% CI, 3.73-16.3) and 0.31 (95% CI, 0.23-0.43), respectively. Conclusion: In patients with testicular cancer, 18F-FDG-PET demonstrated a high SROC area, and could be a potentially useful tool if combined with other imaging methods such as MRI and CT. Nevertheless, the literature focusing on the use of 18F-FDG-PET in this setting still remains limited.

The Effects of Metal Plate loaded on TLD chip in 6 MV Photon and 6 MeV Electron Beams (6 MV 광자선과 6 MeV 전자선 하에서 TLD 기판 위에 얹힌 금속 박막의 효과)

  • Kim, Sookil;Byungnim Min
    • Progress in Medical Physics
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    • v.10 no.1
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    • pp.41-46
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    • 1999
  • There is necessity for making a smaller and more sensitive detector in small field sizes. This report assesses the suitability of metal-loaded thermoluminescent dosimeters for this purpose. Measurements were performed in the 6 MV photon and 6 MeV electron beams of a medical linear accelerator with LiF thermoluminescence dosimeters (TLD-100) embedded in solid water phantom. TLD-100 chips(surface area 3.2 $\times$ 3.2 $\textrm{mm}^2$) loaded with a metal plate(Tin or gold respectively) were used to enhance dose readings to TLD-100. Surface dose was measured for field size 10 $\times$ 10 $\textrm{cm}^2$ and 100 em SSD. Measurements have been made of the enhanced signal intensity and good linearity for absorbed dose with each metal. Using a 1 mm each metal on TLD-l00 in the beam increased the surface dose to 14% and 56% respectively for 6MV photon. In the case of 6 MeV electron, gold plate enhanced the TL response to 13%, but there is no difference for tin plate. The specific dose response of TLD-100 with thin metal plate increases with electron concentration of metal film, this is most likely due to increased electron scattered from the additional material with electron density higher than TLD-100. This emphasizes the role of TL dosimeters with metal as amplified dosimeters for therapeutic high energy x-ray beams. Due to the enhanced dose reading of TLD-100 with metal plate, it could be possible to develop smaller TL dosimeter with high sensitivity.

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Genetic Variation in Flammulina velutipes (팽이버섯의 유전적 변이)

  • Kim, Jong-Bong;Jeong, Ja-In
    • Journal of Life Science
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    • v.21 no.10
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    • pp.1434-1442
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    • 2011
  • A genetic variation within 29 strains of F. velutipes was analyzed by internal transcribed spacer (ITS) sequence analysis and random amplified polymorphic DNA (RAPD). Seven hundred and twenty base pairs were sequenced during the analysis of the ITS region, but no significant variation was observed among the 29 strains of F. velutipes. Sixteen out of 40 random primers amplified polymorphic RAPD fragment patterns. The polymorphic levels of RAPD bands by some primers (OPA-2,4,3,9,10,20) were very high in all 29 strains, with 3,030 fragments ranging between 200 and 2,000 bp. Intraspecific genetic dissimilarity of the 29 strains was calculated to range from 3.3% to 45% by Nei-Li's method using these 3,030 RAPD bands. The genetic variation among Korean strains was relatively high, with dissimilarities ranging between 17% and 38.6%. In the Neighbor-Joining analysis using the genetic dissimilarities based on RAPD, all 29 strains were classified into 5 clusters. Strains in each cluster showed specific characteristics according to their origin and strains. These results suggested that OPA and OPB primers could be used for developing molecular genetic markers and screening of unidentified (F. velutipes) strains.