• 원예과학기술지
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• 제16권1호
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• pp.27-29
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• 1998

인공수분 및 promalin 처리가 사과 후지품종의 정형과 생산에 미치는 영향을 알아보기 위하여 5년생의 후지/M26/실생을 공시하여 과실비대 효과 빛 과형분포 정도를 조사한 결과, 인공수분+promalin 처리구가 다른 처리구 보다 종경, 과중, L/D비가 높았으며, 정형과 비율은 78.6%로서 무처리에 비해 약 30% 정도 높은 상품과를 생산할 수 있는 것으로 나타났다. 특히 장원형 과실의 특정인 apex 생장량이 높아 과중과 과실 외관이 우수하였다. 인공수분+promalin 처리구의 종자수 및 종자중에 있어서는 무처리에 비해 각각 1.5개, 0.14g 정도 높아 과실비대가 좋은 경향이었다. 그러나 promalin 단용처리의 경우, 단위결과에 의한 배주의 퇴화 및 장타원형의 비정형과실이 일부 발생되는 문제점이 있어, 인공수분 또는 방화곤충 활용을 적극적으로 수행한 후 promalin을 살포하는 것이 기형과실을 줄일 수 있는 방안이라 판단된다. Promalin 처리에 따른 과실 품질 및 숙기 정도에는 영향이 없었다.

• 원예과학기술지
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• 제19권3호
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• pp.315-319
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• 2001

본 연구는 배추(Brassica campestris L. ssp. pekinensis)의 자엽 및 하배축 조직으로부터 효율적인 식물체 재분화 체계를 확립하기 위하여 실시하였다. 다양한 조합의 식물생장조절제 NAA와 BA를 조사한 바 자엽은 NAA 2.0mg/L, BA 1.0mg/L와 $AgNO_3$ 16.7mg/L를 첨가한 배지에서 절편당 2.67개의 가장 좋은 신초발생을 보였다. 하배축 절편체로부터는 NAA 1.0mg/L, BA 5.0mg/L와 $AgNO_3$ 16.7mg/L를 첨가하였을 때 절편당 1.87개의 가장 좋은 신초발생을 보였다. 재분화된 신초는 발근배지에서 뿌리를 유기하였으며 식물체는 습도가 유지된 생장상에서 순화를 거처 온실에서 재배하였다. 재분화 개체는 표현형과 염색체수에서는 이상이 없는 것으로 나타났다.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.173-178
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• 2010

An efficient protocol for shoot regeneration was developed for sesame (Sesamum indicum L.) internodes using the transverse thin cell layer (tTCL) culture method. The frequency of shoot regeneration and the number of adventitious buds produced from regenerated shoots depend significantly on explant age, thickness of the tTCL sections, and the phytohormones supplemented to the culture medium. A combination of 6-benzyladenine (2.0 $mg\;l^{-1}$) and a-naphthaleneacetic acid (0.5 $mg\;l^{-1}$) was found to be the best phytohormone combination for shoot bud induction, with the maximum number of shoots obtained when the tTCL sections were 0.5-1.0 mm thick and derived from 4- to 6-week-old seedlings of sesame. Well-developed shoots were rooted on MS medium without phytohormones, and 80% of the regenerated plantlets were successfully established in soil.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.213-218
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• 2008

Swertia chirata is an endangered gentian species that prefers to grow at higher altitudes. This ethnomedicinal herb is known primarily for its bitter taste caused by the presence of important phytochemicals that are directly associated with human health benefits. Due to a continuous loss of habitat and inherent problems of seed viability and seed germination, alternative strategies for propagation and conservation are urgently required to prevent the possible extinction of this species. We have formulated a reproducible protocol for the rapid propagation and conservation of this plant using leaves taken from in vitro shoot cultures. Direct induction of more than seven shoot buds per explant was achieved for the first time when the explants were placed on MS medium supplemented with $2.22{\mu}M$ N-6-benzyladenine, $11.6{\mu}M$ kinetin, and $0.5{\mu}M$ ${\alpha}-naphthalene$ acetic acid. Direct organogenesis was noted exclusively from the adaxial surface of the basal segments of leaves. Leaves closer to the apical meristem were more responsive than those farther away from the meristem. Plants raised through direct organogenesis were evaluated for their clonal fidelity by chromosomal analysis and DNA fingerprinting. Complete plants were successfully transferred to the field condition and produced viable seeds. Given the enormous potential of this age-old medicinal plant in terms of potential health-benefitting drugs, this protocol can be used for commercial propagation purposes and to initiate future genetic improvement studies.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.113-118
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• 2004

Somatic embryos were formed from calli obtained from axillary shoots (raised from nodal segments of glasshouse-grown plants under aseptic conditions), internodal segments (from in vitro-raised plants), and root and coty-ledonary leaf segments (from in vitro-raised seedlings) after 8 weeks of initial culture. Embryo formation was the highest (97.33%) from cotyledonary leaf callus on Mura-shige and Skoog's (MS) medium containing kinetin (KN) (3 mg/L). Somatic embryo induction was lesser with different combinations of auxins while it increased to 100% in internodal segment and cotyledonary leaf calli with 6-benzyladenine (BA) (2mg/L) along with 2,3,5-triiodobenzoic acid (TIBA) (2mg/L). The shoots were induced from somatic embryos raised from root, coty-ledonary leaf and internodal segment calli grown on MS medium containing BA in combination with indole-3-acetic acid (IAA). Maximum of 66.67% cultures formed shoots on MS medium containing BA (1mg/L) in combination with IAA (2mg/L). The shoots raised from somatic embryos were rooted on MS medium supplemented with indole-3-butyric acid (IBA) (2mg/L). The plantlets transferred to the field showed 70% survival rate after one year.

• 원예과학기술지
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• 제32권5호
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• pp.694-701
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• 2014

Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots ($93.33{\pm}4.63%$), roots ($76.67{\pm}7.85%$), and calli ($80.00{\pm}7.43%$) when cultured on Gamborg B5 (B5) medium containing $10{\mu}M$ 6-benzylaminopurine (BA) + $1{\mu}M$ naphthalene acetic acid (NAA), $0.5{\mu}M$ BA + $5{\mu}M$ NAA, and $1{\mu}M$ BA + $10{\mu}M$ NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to $10{\mu}M$. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.

• 원예과학기술지
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• 제32권5호
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• pp.577-583
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• 2014

본 연구는 '홍로'와 '후지' 품종에 benzyladenine(BA, 99% purity), MaxCel$^{(R)}$(1.9% BA), Fruitone(3.5% NAA), MaxCel$^{(R)}$ + Fruitone, simazine 등 약제들이 과실의 착과 및 과실품질에 미치는 영향을 조사하였다. BA와 MaxCel$^{(R)}$은 $100mg{\cdot}L^{-1}$ 농도로, Fruitone은 $0.1mg{\cdot}L^{-1}$ 농도로 과실직경이 6mm인 만개 8일 후에, 그리고 simazine은 $400mg{\cdot}L^{-1}$ 농도로 만개 7일후와 14일 후에 2회 처리하였다. 적과제 처리 후 '홍로' 품종의 정화아의 과총당 착과수는 MaxCel$^{(R)}$ + Fruitone, MaxCel$^{(R)}$, simazine 처리구가 각각 1.67, 1.84, 1.81개로 무처리구 2.35개보다 적어 적과효과를 보였고, 특히 무착과 과총 비율이 높아 적과효과가 더 우수하였다. '후지' 품종의 경우 정화아의 과총당 착과수는 MaxCel$^{(R)}$ + Fruitone, Fruitone, MaxCel$^{(R)}$ 처리구가 1.29, 1.60, 1.76개로 무처리구 2.56개보다 적어 우수한 적과효과를 보였다. 또한, 두 품종 모두에서 MaxCel$^{(R)}$ + Fruitone 혼용처리구가 MaxCel$^{(R)}$ 단독처리구에 비하여 적과증진효과를 보였다. 그리고 액화아의 경우도 정화아의 결과와 유사한 결과를 보였다. 과중은 '홍로' 품종의 BA 처리구에서, '후지' 품종에서는 BA와 MaxCel$^{(R)}$ 처리구에서 증가하였다. 그리고 가용성 고형물 함량은 두 품종 모두에서 BA, MaxCel$^{(R)}$, MaxCel$^{(R)}$ + Fruitone 처리 과실들에서 증가하였지만 다른 과실특성들은 차이를 보이지 않았다.

• 한국환경농학회지
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• 제16권3호
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• pp.227-232
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• 1997

UV-B 조사에 의한 오이의 생장 및 생리 기능에 미치는 영향을 조사하기 위하여 비료처리에 의한 오이 제1 엽의 UV-B의 영향을 조사하였다. UV-B 조사에 의한 가시장해는 처리 1주일부터 나타났으나 생장 저해 현상은 볼 수 없었다. 그러나 UV-B 조사에 의한 생장 저해 현상은 비료 첨가에 의해 나타났으며 가장 큰 저해 효과는 질소 시용에 의해 나타났다. 이 결과는 UV-B가 질소대사에 관여하고 있음을 시사한다. 따라서 질소대사에 관여하고 있는 주 효소인 질산 환원효소 활성을 조사한 결과 UV-B 조사 7일째에 비료 처리구에서 현저히 증가하는 경향을 나타냈다. 또한 UV-B 조사에 의한 엽면적의 생장감소는 세포의 신장, 분열 생장에 관여하고 있는 식물호르몬 활성에 영향을 미치는 것으로 보여 오이의 제 1엽에서의 BA 처리에 의한 사이토카이닌의 감수성을 조사했다. UV-B 조사에 의한 사이토카이닌의 감수성은 UV-B를 조사하지 않은 구에 비해 억제되었다. 이 결과에 의해 UV-B 조사가 식물 생장에 관여하고 있는 사이토카이닌의 활성을 억제시켜 엽면적의 감소가 발생된 것으로 생각되었다.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.129-138
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• 2010

An efficient transformation system for high-throughput functional genomic studies of kiwifruit has been developed to overcome the problem of necrosis in Actinidia arguta explants. The system uses Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pART27-10 to inoculate leaf strips. The vector contains neomycin phosphotransferase (nptII) and ${\beta}$-glucuronidase (GUS) (uidA) genes. A range of light intensities and different strengths of Murashige and Skoog (MS) basal salt media was used to overcome the problem of browning and/or necrosis of explants and calli. Callus browning was significantly reduced, resulting in regenerated adventitious shoots when the MS basal salt concentration in the culture medium was reduced to half-strength at low light intensity ($3.4\;{\mu}mol\;m^{-2}\;s^{-1}$) conditions. Inoculated leaf strips produced putative transformed shoots of Actinidia arguta on half-MS basal salt medium supplemented with 3.0 $mg\;l^{-1}$ zeatin, 0.5 $mg\;l^{-1}$ 6-benzyladenine, 0.05 $mg\;l^{-1}$ naphthalene acetic acid, 150 $mg\;l^{-1}$ kanamycin and 300 $mg\;l^{-1}$ $Timentin^{(R)}$. All regenerated plantlets were deemed putativ transgenic by histochemical GUS assay and polymerase chain-reaction analysis.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.43-50
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• 2006

The goal of this research was to develop an efficient cryopreservation protocol for germplasms of yam (Diosorea batatas), that were cultivated in Korea. Comparative studies with four other cryogenic techniques and subsequent experiments for shoot regrowth were conducted. in vitro-grown shoot-apices of the D. batatas were successfully cryopreserved by encapsulation-dehydration. The maximum survival of shoot-apices could be achieved when the precultured (with 0.3 M of sucrose for one day) and encapsulated (with a 3%(w/v) Na-alginate solution) apices were dehydrated for $3.5{\sim}4\;h$ prior to direct immersion in LN (liquid nitrogen). The frequency of regrowth rate of cryopreserved apices was not decreased during 3-month storage period. The thawing method markedly affected survival of the cryopreserved apices, and thawing at $40^{\circ}C$ for 3 min produced the best results. When cryopreserved apices were post-cultured on the post-culture medium (MS), supplemented with $0.2mgl^{-1}$ of BA ($N_6$-benzyladenine) and $0.2mgl^{-1}$ of kinetin, they showed direct shooting without callusing.