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Somatic Embryogenesis in Withania somnifera (L.) Dunal  

Rani, Gita (Department of Botanical and Environmental Sciences, Guru Nanak Dev University)
Virk, Gurdip Singh (Department of Botanical and Environmental Sciences, Guru Nanak Dev University)
Nagpal, Avinash (Department of Botanical and Environmental Sciences, Guru Nanak Dev University)
Publication Information
Journal of Plant Biotechnology / v.6, no.2, 2004 , pp. 113-118 More about this Journal
Abstract
Somatic embryos were formed from calli obtained from axillary shoots (raised from nodal segments of glasshouse-grown plants under aseptic conditions), internodal segments (from in vitro-raised plants), and root and coty-ledonary leaf segments (from in vitro-raised seedlings) after 8 weeks of initial culture. Embryo formation was the highest (97.33%) from cotyledonary leaf callus on Mura-shige and Skoog's (MS) medium containing kinetin (KN) (3 mg/L). Somatic embryo induction was lesser with different combinations of auxins while it increased to 100% in internodal segment and cotyledonary leaf calli with 6-benzyladenine (BA) (2mg/L) along with 2,3,5-triiodobenzoic acid (TIBA) (2mg/L). The shoots were induced from somatic embryos raised from root, coty-ledonary leaf and internodal segment calli grown on MS medium containing BA in combination with indole-3-acetic acid (IAA). Maximum of 66.67% cultures formed shoots on MS medium containing BA (1mg/L) in combination with IAA (2mg/L). The shoots raised from somatic embryos were rooted on MS medium supplemented with indole-3-butyric acid (IBA) (2mg/L). The plantlets transferred to the field showed 70% survival rate after one year.
Keywords
Embryogenic callus; somatic embryogenesis; Withania somnifera;
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