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Examination of the Presence and Differential Expression of Connexin Isoforms in the Urinary Bladder of the Male Rat During Postnatal Period

  • Lee, Ki-Ho
    • Journal of Animal Science and Technology
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    • v.54 no.6
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    • pp.419-425
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    • 2012
  • The coordinated action of the detrusor muscle cells in the urinary bladder is governed by cell-cell communication through gap junction, consisted of connexin (Cx) molecules. Even though a number of researches have been mostly focused on expressional changes of a few Cx isoforms in clinically dysfunctional condition of the bladder, less attention has been paid for investigation of Cx isoforms present in the bladder. Using real-time PCR analysis, the present study examined Cx isoforms expressing in the male rat bladder during postnatal period. Also, expressional patterns of Cx isoforms were evaluated in the bladder at different postnatal ages. Of a total of 13 Cx isoforms tested in the present study, we were able to detect mRNAs of 6 Cx isoforms in the rat urinary bladder, including Cxs 31, 31.1, 32, 37, 40, and 45. The transcript levels of Cxs 31, 31.1, 37, 40, and 45 were gradually increased from 1 week of age until 25 days of age, followed by transient decreases at 45 days of age. However, abundance of Cx32 transcript was drastically increased at 15 days of age, followed by a sharp drop at 45 days of age. These results indicate that differential expression of Cx isoforms in the bladder during postnatal development would be necessary for maintaining proper function of the bladder. A question remains to be answered if significant decreases of transcript levels of some Cx isoforms at the elderly are associated with age-dependent dysfunction of the bladder.

Isotope Measurement of Uranium at Ultratrace Levels Using Multicollector Inductively Coupled Plasma Mass Spectrometry

  • Oh, Seong-Y.;Lee, Seon-A.;Park, Jong-Ho;Lee, Myung-Ho;Song, Kyu-Seok
    • Mass Spectrometry Letters
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    • v.3 no.2
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    • pp.54-57
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    • 2012
  • Mass spectrometric analysis was carried out using multicollector inductively coupled plasma mass spectrometry (MC-ICP-MS) for the precise and accurate determination of the isotope ratios of ultratrace levels of uranium dissolved in 3% $HNO_3$. We used the certified reference material (CRM) 112-A at a trace level of 100 pg/mL for the uranium isotopic measurement. Multiple collectors were utilized for the simultaneous measurement of uranium isotopes to reduce the signal uncertainty due to variations in the ion beam intensity over time. Mass bias correction was applied to the measured U isotopes to improve the precision and accuracy. Furthermore, elemental standard solution with certified values of platinum, iridium, gold, and thallium dissolved in 3% $HNO_3$ were analyzed to investigate the formation rates of the polyatomic ions of $Ir^{40}$ $Ar^+$, $Pt^{40}$ $Ar^+$, $Tl^{40}$ $Ar^+$, $Au^{40}$ $Ar^+$ for the concentration range of 50-400 pg/mL. Those polyatomic ions have mass-to-charge ratios in the 230-245 m/z region that it would contribute to the increase of background intensity of uranium, thorium, plutonium, and americium isotopes. The effect of the polyatomic ion interference on uranium isotope measurement has been estimated.

PDOCM : Fast Text Compression on MasPar Machine (PDOCM : MasPar머쉰상의 새로운 압축기법과 빠른 텍스트 축약)

  • Min, Yong-Sik
    • The Journal of the Acoustical Society of Korea
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    • v.14 no.1
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    • pp.40-47
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    • 1995
  • Due to rapid progress in data communications, we are able to acquire the information we need with ease. One means of achieving this is a parallel machine such as the MasPar. Although the parallel machine makes it possible to receive/transmit enormous quantities of data, because of the increasing volume of information that must be processed, it is necessary to transmit only a minimal amount of data bits. This paper suggests a new coding method for the parallel machine, which compresses the data by reducing redundancy. Parallel Dynamic Octal Compact Mapping (PDOCM) compresses at least 1 byte per word, compared with other coding techniques, and achieves a 54.188-fold speedup with 64 processors to transmit 10 million characters.

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Freeze drying of a Wooden Comb Excavated at Shinchang-dong Wetland Site with Cetylalcohol (세틸알콜을 이용한 광주 신창동 저습지 출토 목제 빗의 동결건조)

  • Park, Youngman
    • Conservation Science in Museum
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    • v.11
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    • pp.49-52
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    • 2010
  • A wooden Comb excavated at Shinchang-dong wetland site was treated with cetylalcohol as a reinforcing agent. The conservation process was done by freeze drying the wooden artifact after substituting it with no.3 butyl alcohol and impregnating with 40wt% cetylalcohol solution. Consequently there was no noticeable surface damage. Also it showed 54% of remaining weight and a shrinkage of length 3.3%, width 2.1% and thickness 3.1%. Therefore using cetylalcohol for conservation of waterlogged archeological wood was effective dimensional stability and remaining weight.

Solidification Cracking Behavior in Austenitic Stainless Steel Laser Welds (Part 1) - Evaluation of Solidification Cracking Susceptibility by Laser Beam Welding Varestraint Test - (오스테나이트계 스테인리스강 레이저 용접부의 응고균열 거동 (Part 1) - 레이저 용접용 Varestraint 시험 시스템을 이용한 응고균열 민감도 평가 -)

  • Chun, Eun-Joon;Lee, Su-Jin;Suh, Jeong;Kang, Namhyun;Saida, Kazuyoshi
    • Journal of Welding and Joining
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    • v.34 no.5
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    • pp.54-60
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    • 2016
  • In order to quantitatively evaluate the solidification cracking susceptibility in laser welds of three types of austenitic stainless steels (type 310: A mode, type 316-A: AF mode, type 316-B: FA mode solidifications), the laser beam welding (LBW) transverse-Varestraint tests consisted of multi-mode fiber laser, welding robot and hydraulic pressure system were performed. As the welding speed increased from 1.67 to 40.0 mm/s, the solidification brittle temperature range (BTR) of laser welds for type 316 stainless steels enlarged (316-A: from 37 to 46 K, 316-B: from 14 to 40 K), while the BTR for type 310 stainless steel reduced from 146 to 120 K. In other words, it founds that solidification cracking susceptibility could not be simply mitigated through application of LBW process, and the BTR variation behavior is quite different upon solidification mode of austenitic stainless steels.

A case of tacrolimus-induced encephalopathy after kidney transplantation

  • Kim, Myoung-Uk;Kim, Sae-Yoon;Son, Su-Min;Park, Yong-Hoon
    • Clinical and Experimental Pediatrics
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    • v.54 no.1
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    • pp.40-44
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    • 2011
  • We present a case of tacrolimus-induced encephalopathy after successful kidney transplantation. An 11-year-old girl presented with sudden onset of neurologic symptoms, hypertension, and psychiatric symptoms, with normal kidney function, after kidney transplantation. The symptoms improved after cessation of tacrolimus. Magnetic resonance imaging (MRI) showed acute infarction of the middle cerebral artery (MCA) territory in the right frontal lobe. Three days later, she had normal mental function and maintained normal blood pressure with left hemiparesis. Follow-up MRI was performed on D19, showing new infarct lesions at both cerebral hemispheres. Ten days later, MRI showed further improvement, but brain single photon emission computed tomography (SPECT) showed mild reduction of uptake in both the anterior cingulate gyrus and the left thalamus. One month after onset of symptoms, angiography showed complete resolution of stenosis. However, presenting as a mild fine motor disability of both hands and mild dysarthria, what had been atrophy at both centrum semiovale at 4 months now showed progression to encephalomalacia. There are two points of interest in this case. First, encephalopathy occurred after administration of tacrolimus and improved after discontinuation of the drug. Second, the development of right-side hemiplegia could not be explained by conventional MRI; but through diffusion tensor imaging (DTI) and diffusion tensor tractography (DTT) of white matter tract, visualization was possible.

$\beta$-COMPACTNESS IN L-FUZZY TOPOLOGICAL SPACES

  • Cho, S.H;Kim, M.Y
    • Journal of applied mathematics & informatics
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    • v.9 no.1
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    • pp.359-370
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    • 2002
  • The purpose of this paper is to introduce and discuss the concept of $\beta$-compactness for L-fuzzy topological spaces.

Leaching of $^{54}Mn$, $^{60}Co$, $^{85}Sr$ and $^{137}Cs$ Deposited to Paddy Soil during the Growing Season of Rice (벼 재배기간 중 논 토양에 처리한 $^{54}Mn$, $^{60}Co$, $^{85}Sr$, $^{137}Cs$의 용탈)

  • Choi, Yong-Ho;Jo, Jae-Sung;Lee, Chang-Woo;Chung, Kyu-Hoi;Lee, Jeong-Ho
    • Korean Journal of Environmental Agriculture
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    • v.15 no.2
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    • pp.198-206
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    • 1996
  • Leaching of $^{54}Mn$, $^{60}Co$, $^{85}Sr$ and $^{137}Cs$ in paddy soil was studied using lysimeter cultures in a greenhouse. The radionuclides were applied to the water surfaces shortly before transplanting and five different times between transplanting and harvest. Fertilizer KCl and slaked lime were added simultaneously in the rate of 83kg and 200kg, respectively, per l0a following the first application after transplanting. Percolating water was sampled until two days before harvest. Concentrations of the radionuclides in percolating water decreased in the order of $^{85}Sr$ > $^{54}Mn$ > $^{60}Co$ > $^{137}Cs$ on the whole. Time taken to reach the maximum was the shortest for $^{137}Cs$(< one week) and the longest for $^{54}Mn$ and $^{85}Sr$. Six days' water dropping started 47 days after transplanting reduced the concentrations of $^{54}Mn$, $^{60}Co$, $^{85}Sr$ and $^{137}Cs$ by factors of 30-180, 3-75, 2-4 and 3-6, respectively, depending on the application time. After the significant decrease, $^{54}Mn$ concentration tended to gradually increase but $^{137}Cs$ did to the contrary Percent leaching varied 0.09-6.2% for $^{54}Mn$, 0.009-0.9% for $^{60}Co$, 1.4-14.4% for $^{85}Sr$ and 0.002-0.06% for $^{137}Cs$, with the application time. The highest leaching came from the application at 40 days after transplanting for all the radionuclides. The addition of KCl and lime increased percent leaching of the radionuclides by factors of 9, 85, 4 and 9, respectively.

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A Study on Non-smoking Education status through School Health Care Services (학교보건간호를 통한 금연 교육의 실태에 관한 연구)

  • Chung, Yeon Kang;Chang, Young Mee
    • Journal of the Korean Society of School Health
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    • v.7 no.2
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    • pp.135-143
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    • 1994
  • This study is aimed at providing basic information necessary to set up education program and strategies to prevent high school students from smoking by school health care service. The main groups of study are 814 third-grade male students, 557 parents and 362 teachers, who were randomly chosen at 8 high schools in Seoul. Date analysis consisted of Chi-square test and percentage. The findings of the study are as follows: 1. The less interesting family life is, the more increasing current smoking rate is, (ex-smoking rate 53.5%, re-smoking rate 40.3%, current smoking rate 24.3%) 2. According th the results of $x^2$-test for the school life and the smoking, the students above ranking 41 showed th increase as 48.7% for continuous smoking, 18.9% for re-smoking, 67.6% for the present smoker. But it turned out that the students belonged to ranking 10 were not experienced the smoking. So it showed that there was a correlation between score and smoking. 3. It is noted that 93.3% of students, 93.3% of parents and 96.1% of teachers recognize harmful effect of smoking. But less than 70% those have recognized only half of all smoking knowledge. 4. There is a significant difference in the contends and types of education between parents and teachers. 5. For the time of home education and school health education to prevent the smoking, it turned out that 44.9% of students, 42.4% of parents, and 47.4% of teachers considered the optimal time as a high school' days. In addition it appeared that 40.5% of students, 33.4% of parents, and 54.6% of teachers recognized the necessity of the early education before the elementart school. For the optimal time to begin school health education, it showed that the middle school days were indicated from 56.6% of students, 52.7% of parents, the elementary school days were 54.6% of parents, the elementary school days were 54.6% of teachers.

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