• Title/Summary/Keyword: 53C30

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Cytosine Deaminase of Fungus (곰팡이의 Cytosine Deaminase에 관한 연구)

  • ;;Takuo Sakai;Kenzo Tonomura
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.169-174
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    • 1986
  • Cytosine deaminase was partialy purified about 10 fold from the ceil-free extract of Aspergillus fumigatus. The partialy purified enzyme was relatively stable in a pH 5.5 to 8.0, but thermo-unstable. The enzyme activity was found in a pH optimum of 7.0 and temperature optimum of 30 to 35$^{\circ}C$. The activation energy calculated to be 13,240 cal/mol. The apparent Michaelis constants Km for cytosine was found to be 1.53 mM and the molecular weight was determined to be approximately 32,000. The enzyme was strongly inhibited by 0.1 mM of Hg$^^{2+}$, Pb$^{2+}$, Cd$^{2+}$ and Fe$^{2+}$, furthermore inhibited by 1mM of ATP, UTP, o-phenanthroline and p-chloromercuribenzoate.

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Separation of 6-Gingerol from Ginger [Zingiber officinale Roscoe] and Antioxidative Activity (생강으로부터 6-Gingerol의 분리 및 항산화 활성)

  • Lee, Bong-Soo;Ko, Meyong-Seok;Kim, Hyun-Jong;Kwak, In-Seob;Kim, Dong-Ho;Chung, Bong-Woo
    • KSBB Journal
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    • v.21 no.6 s.101
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    • pp.484-488
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    • 2006
  • Ginger (Zingiber officinale) is widely used as a dietary condiment throughout the world. Its major constituent, 6-gingerol, exhibits diverse pharmacological activities including anti-oxidant and anti-tumor. Ginger were extracted by 0% to 95% ethanol. Maximum yield of 6-gingerol was obtained with 80% ethanol as extracting solvent at $30^{\circ}C$. We obtained increased yield (7%) of extraction by pretreatment with ultrasonication. Gingerols in the crude ginger extract was isolated by Sepacore preparative liquid chromatography on silica gel. We got the 6-gingerol which weight is 0.53 mg/mL, from fraction F9. Antioxidant effect of 6-gingerol were detected by DPPH moth(10. Its radical scavenging activity was $95{\sim}99%$ which compared with ascorbic acid.

STUDY ON THE SPAT COLLECTION OF OYSTER 3. Spat Fall and Exposure Time (참굴 채묘에 관하여 3. 노출 시간과 치패 부착)

  • BAE Gyung-Man
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.5 no.1
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    • pp.23-28
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    • 1972
  • The following results were obtained from the experiment on the oyster spatfall, Crassostrea gigas by means of the rack method in YonghoBay, on the southern coast of Korea, from June to August in 1962 and 1963. 1. The Outside of shells as a collector showed better result than inside in numbers of attached oyster spats. 2. The horizontal type of the rack was more efficient for spatfall than the typical type of the rack. 3. The mode variance, in shell height, were shown as 21-25mm and 26-30mm, and the variance of range was 13.0-53.0mm for 77 days. 4. The optimum spat-fall season was from August 17 to 19 and 24 to 27, and spat of the oyster were attached in number of 7 per collector each day. 5. The optimum exposure time in spatfall was 4 hours. 6, The average water temperature and specific gravity, during the spawning season, was $25.98^{\circ}C$ and 0.1871, respectively.

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Blister blight of Tea (Thea sinensis) Caused by Exobasidium vexans in Korea (Exobasidium vexans에 의한 차나무 떡병)

  • Kwon, Jin-Hyeuk;Jeong, Seon-Gi;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.9 no.4
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    • pp.209-212
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    • 2003
  • Blister blight was occurred on tea (Thea sinensis) in a farmer's tea garden located in Sicheon-myon, Sancheong-gun, Gyeongnam province, Korea. The typical symptoms of the disease were appeared on the leaves and bud. The early symptom were started with small yellowish spots then infected area became upheave and covered with white blister which the hymenium de.veloped from under neath of epidermis. Generally, the infected leaves and buds were appeared like rice cake. Colonies formed on PDA were developed white, later turned milk white. Basidia were 2 sterigma, and 30${\sim}$53 ${\times}$ 3.1${\sim}$5.8 ${\mu}m$ in size. Basidiospores were ellipsoid curved, coloriess, 1-celled and 11${\sim}$14.7 ${\times}$ 4${\sim}$5.4 ${\mu}m$ in size. The optimum temperature for growth of the fungus was about $25^{\circ}C$ on PDA and PSA. This is the first report on the blister blight of tea caused by Exobasidium vexans in Korea.

Measurement of Radon and its Daughters Concentration in Air (공기중 라돈 및 라돈 자핵종의 농도 측정)

  • Park, Y.W.;Ha, C.W.;Ro, S.G.
    • Journal of Radiation Protection and Research
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    • v.14 no.2
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    • pp.23-29
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    • 1989
  • A Lucas cell was established and calibrated by using the double layer tube standard radon source. The calibration factors were 0.031$\pm$0.002 (pCi/l)/(cph/Cell) at room temperature, and 0.029$\pm$0.001 (pCi/l)/(cph/Cell) at $50^{\circ}C$. Radon and its daughters concentrations were measured in a room air for the demonstrating purpose. The concentrations of 222 Rn, $^{218}Po,\;224\;Pb,\;and\;^{214}Bi$ were 0.87, 0.53, 0.35 and 0.26 pCi/l. The total eqilibrium factor was around 0.40 and the WL is $3.33{\times}10^{-3}$, resulting in 30 mrem/yr at this place.

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Synthesis and Characterization of Polyurethane bead/silica Hybrid Composites (폴리우레탄 비드/실리카 복합체의 합성 및 그 특성)

  • Yang, Seung Nam;Yim, Gie Hong;Kim, Nam Ki
    • Applied Chemistry for Engineering
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    • v.18 no.4
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    • pp.386-390
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    • 2007
  • In this study, polyurethane prepolymers were synthesized from polycaprolactonediol (PCDs. M.W. 530, 830, 1000, 1250, and 2000) and polycaprolactonetriol (PCTs. M.W. 300 and 900), and hexamethylenediisocyanate (HMDI). Polyurethane beads was prepared from the different prepolymers by a two-step suspension polymerization. The particle size of polyurethane beads was investigated by particle size analyzer. The beads were $10{\sim}30{\mu}m$ in size. The structure of beads was confirmed by FT-IR spectrometer. Their thermal properties were analyzed by TGA. Glass transition temperatures ($T_g$) of the beads were in the range of $-23{\sim}-53^{\circ}C$ and decreased with the increase of the PCD molecular weight. In order to prevention the cohesion of beads, the beads were coated with tetraethoxysilane (TEOS).

Effects of Repetitive Work and Workload for body While Wearing Firefighting Protective Clothing in hot conditions (고온조건하에서 소방용 보호복 착용시 주기적 작업과 작업강도에 따른 신체영향)

  • Bang, Changhoon
    • Fire Science and Engineering
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    • v.32 no.4
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    • pp.35-41
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    • 2018
  • The purpose of study intends to investigate effects of repetitive work and workload for body while wearing firefighting protective clothing in hot conditions and to provide the basic data for the safety of firefighter. The experimental temperature is WBGT $30^{\circ}C$ and the work intensity is 3, 9 METs. The experiment is processed 15 minutes (Ex1), 15 minutes rest (Rest), 15 minutes (Ex2) in order of time. A comparative experiment was carried out for firefighting school students(9 persons; $24.3{\pm}0.71$ years old) wearing firefighting protective clothing. When workload is increased from 3 METs to 9 METs, mean skin temperature change (33.3%), tympanic temperature change (242.9%), heart rate (36.2%), respiration rate (53.9%), RPE (81.6%), thermal Sensation (20.8%), weight loss (60.0%), glucose (-4.4%), lactate (41.7%) are statistically significantly higher. As a result, it is considered that repetitive work and workload have a strong effect on the body when wearing firefighting protective clothing in hot conditions.

A Study on the Fabrication of Hollow Glass Sphere by Using a Liquid-mix Precursor (혼합액상 전구체를 이용한 유리 중공구체의 제조에 관한 연구)

  • Lee, Yong Bin;Kim, Ki Do;Kim, Hee Taik
    • Applied Chemistry for Engineering
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    • v.10 no.8
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    • pp.1186-1191
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    • 1999
  • By using a liquid-mix precursor, we prepared the hollow glass spheres(HGS) as an additive of polymer compound which are used in the field of modifier, promoter, filler, and reinforcement. Liquid-mix precursor is a mixture of 40% sodium silicate aqueous solution, boric acid as a insolubilizing agent, and urea as a blowing agent. To obtain the precursor particles which are fed into a gas flame furnae, the above liquid-mix precursor was dried in oven and crushed with ball mill. We assumed the size of precursor particles ($53{\sim}63{\mu}m$, $63{\sim}180{\mu}m$), temperature of furnace($800{\sim}1200^{\circ}C$), and amount of urea(0~30 g) as the parameters affecting on the properties of HGS. As a result mean particle size of HGS increases with increasing the temperature of furnace and the amount of urea and with decreasing the size of precursor particles. Also, we found that incresing the amount of urea is related to a decrease of the crush strength of HGS.

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The Effects of Writing Using Media on the Promotion of Creative Convergence Capacity (미디어를 활용한 글쓰기가 창의융합 역량 증진에 미치는 효과)

  • Bang, Sul-Yeong;Je, Nam-Joo
    • Journal of Digital Convergence
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    • v.18 no.12
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    • pp.353-362
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    • 2020
  • This was a single group pretest-protest pre-experimental study designed to find out whether writing using media enhances creative convergence capacity. Data were collected from 30 C university students in C city, from March 1st to July 15th, 2020. Analysis was done using IBM SPSS 25.0 for frequency, percentage, average, standard deviation, and paired t-test. Creative problem solving ability was enhanced by an average of 0,63 points (p<.001), critical thinking tendency by 1.06 points (p<.001), self-leadership by 0,53 points (p<.001), and self-control by an average of 0.51 points, so was statistically significant (p=.001). Writing using media had the effect of improving creativity and integration capabilities. The results of this study are expected to be used as basic data for the development of educational programs for creativity and integration enhancement at university-level. Also, follow-up studies on the effectiveness of writing education by utilizing web media as text and tools simultaneously and customized university-level writing education utilizing media are required.

Total Polyphenol Content and Antioxidative Activities of Mistletoe(Viscum album) Extracts by Supercritical Carbon Dioxide (초임계 이산화탄소를 이용한 겨우살이(Mistletoe, Viscum album) 추출물의 폴리페놀 함량 및 항산화 활성)

  • Jang, Tae-Oh;Yoo, Yang-Hoon;Hwang, Yong-Chul;Kim, Hyun-Ku;Woo, Hyun-Chul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.1
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    • pp.20-24
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    • 2010
  • This study was performed to investigate antioxidant activities of Mistletoe (MS) extracts by measuring electron donating ability (EDA), super oxide dismutase (SOD)-like activity and total polyphenol content (TPC). The extracts were obtained using supercritical carbon dioxide, hot-water and ethanol extraction. EDAs was the higher in ethanol extract (70.53%) than water (36.73%) or supercritical carbon dioxide extract (15.73% at 400 bar, $30^{\circ}C$) at concentration of 10 mg/mL. However, SOD-like activities and TPCs were the highest in the supercritical carbon dioxide extract. At concentration of 1 mg/mL, supercritical carbon dioxide extract (22.54% at 200 bar, $40^{\circ}C$) was higher SOD activity than water (7.85%) and ethanol extract (6.12%). Regarding TPCs of MS extracts, the content was the highest in supercritical carbon dioxide extract (17.57 wt% catechin equivalent at 200 bar, $50^{\circ}C$) followed by ethanol and water extract. These results suggest that it can be useful to develop functional foods using antioxidative active compounds of MS with high polyphenol contents.