• Journal of Ginseng Research
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• v.25 no.3
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• pp.136-140
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• 2001

Effects of incubation temperature and pH on chlamydospore germination of Cylindrocarpon destrcutans (isolate CY-9802) causing root rot of Panax ginseng were studied. Germination rate of the chlamydospores on Czapek solution agar(CSA) was higher than on potato dextrose agar(PDA) at the incubation temperatures tested. The chlamydospores were able to be germinated at range of 5$\^{C}$ to 30$\^{C}$ after 48 hours incubation on CSA. Germination rate was 53.2∼6.27% at range of 15$\^{C}$ to 25$\^{C}$, and the optimum temperature was 20$\^{C}$, whereas they were very low at 30$\^{C}$ on PDA. Germination rate was 43.6% to 47.9% at range of 10$\^{C}$ to 20$\^{C}$, and the optimum temperature was 20$\^{C}$ as well. They were able to be germinated at pH of 5.2 to 8.1 on CSA and 5.2 to 7.2 on PDA. Optimum pHs for the germination on CSA and PDA were from 6.4 to 8.2 and from 5.2 to 6.0, respectively. Mycelial color of the fungus on CSA was pale brown at pH from 5.2 to 6.0 and white from pH 6.4 to 8.1, while it was typical dark brown ar range of pH 5.2 to 7.1 and brown at pH 7.2 on PDA after 21 days incubation.

• Korean Journal of Food Science and Technology
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• v.38 no.3
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• pp.414-418
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• 2006

The physicochemical characteristics and sensory properties of Jinyangju, a Korean traditional rice wine brewed using unique glutinous rice, were investigated over the following range of fermentation temperatures: 10, 15, 20 and $25^{\circ}C$. The pH value, total acidity, total sugar content, alcohol content and color value of the Jinyangju were determined after the 2-week fermentation, followed by sensory evaluation of the final product. After fermentation for 2 weeks at temperatures of 10, 15, 20 and $25^{\circ}C$, the final pH values were 3.70, 3.73, 3.40 and 3.26, the final total acidities were 1.04, 1.01, 1.39 and 1.72%, the final total sugar contents were 6.43, 6.22, 5.91 and 5.53% and the final alcohol contents were 14.20, 14.17, 14.83 and 15.40%, respectively. The color value of the Jinyangju was not different among the samples. In conclusion, the Jinyangju fermented at $15^{\circ}C$ showed the highest values in such sensory properties as color, flavor and odor, and was judged in the sensory evaluation to be the overall favorite.

• Korean Journal of Weed Science
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• v.31 no.1
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• pp.78-83
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• 2011

This study was conducted to evaluate germination rate of Vicia tetrasperma and V. hirsuta as affected by storage duration, temperature, and light. All seeds of both species were gathered from Dangjin, Chungnam in the late June of 2004 and 2005, stored for a certain period of time, and then exposed for 20 days to the alternating temperatures ($15/^{\circ}C,\;20/10^{\circ}C,\;25/15^{\circ}C$, and $30/20^{\circ}C$) for evaluating the germination rate. Both 2004 and 2005, two species stored at room temperature for 3 months had germination rates less then 30% with applying various alternating temperatures for 20 days although the highest germination rate was obtained with $25/15^{\circ}C$ treatment. V. tetrasperma at 12 month storage after seed gathering in 2005 had 82% of germination rate with $25/15^{\circ}C$ treatment and showed the increased rate as time passed. V. hirsuta at 6, 9, and 12 month storage after seed gathering in 2005 ranged, however, from 33 to 53% of the germination rate at alternating temperature treatments for 20 days and did not seem to be affected by the elapsed time of the storage. Storage at room temperature was more effective for increasing germination rate of both species compared to those of the storage at low temperature. The germination rate of both species was not significantly affected by either exposure of light or short period of high temperature.

• Korean journal of applied entomology
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• v.24 no.3 s.64
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• pp.141-149
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• 1985

Embryonic developmental rates of the Oriental tobacco budworm, Heliothis assulta Guenee, were compared at various constant temperatures and 16 hours of light, and detailed embryogenesis was also studied at $25^{\circ}C$. The egg was nearly globular in form and had an average equatorial diameter of 0.53mm. A single micropyle was in the center of the circular area at the anterior pole of the egg. Durations of embryogenesis at 20, 25, 30, and $35^{\circ}C$ were 147, 81, 61, and 67hrs, respectively. Embryonic death was especially higher at $35^{\circ}C$ than any other temperatures investigated. Embryogenesis progressed with changes in color and pattern, which are quite characteristic at each developmental stage of the embryo. At $25^{\circ}C$, organogenesis began in 14hrs after oviposition, formation of gut completed in 44hrs and eclosion occurred in 80hrs. The embryo formed along the long axis at early developmental stage, moved towards the equatorial plane in 24hrs, and made a half-turn on the plane in 36hrs. In 40hrs, head was oriented to the anterior pole of the egg until eclosion.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.2
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• pp.103-107
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• 2000

The viviparity of 28 rice varieties was tested at 25 days after heading(DAH), 35DAH, and 45DAH in the laboratory and field condition for 12 days. The incubation temperature was 20/l$0^{\circ}C$ (day/night), 25/15$^{\circ}$C$ and 30/20$^{\circ}$C$ in the laboratory test, and under field water conditions in the field test. The biggest varietal difference of viviparity was found in the laboratory test when examined at 45DAH with the 6-day incubation under 25/15$^{\circ}$C$ . At this conditions the mean viviparous ratio was 32.1 % with the range of 53.9 and the variance of 259.5. In the field test, the significant varietal difference in the viviparity was also found in the lodging treatment at 45 DAH for 6 days. Correlation coefficient analysis between the field and laboratory tests was highly significant from 4 days after incubation at 45 DAH and after 6-day incubation at 35 DAH, and correlation coefficient was higher as incubation days in the laboratory and submerged days under field water became longer. Considering the correlation between the field and laboratory tests, varietal difference of viviparity and convenience of testing, the laboratory test at 45 DAH for 6-day incubation under 25/15$^{\circ}$C$ was the most efficient evaluation method for the viviparity of rice cultivar.

• IMMUNE NETWORK
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• v.9 no.2
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• pp.58-63
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• 2009

Background: T cell immunoglobulin and mucin domain containing 3 protein (Tim-3) expressed on terminally differentiated Th1 cells plays a suppressive role in Th1-mediated immune responses. Recently, it has been shown that N-glycosylation affects the binding activity of the Tim-3-Ig fusion protein to its ligand, galectin-9, but the binding properties of non-glycosylated Tim-3 on $CD4^+CD25^+$T cells has not been fully examined. In this study, we produced recombinant Tim-3-Ig fusion proteins in different cellular sources and its N-glycosylation mutant forms to evaluate their binding activities to $CD4^+CD25^+$T cells. Methods: We isolated and cloned Tim-3 cDNA from BALB/C mouse splenocytes. Then, we constructed a mammalian expression vector and a prokaryotic expression vector for the Tim-3-Ig fusion protein. Using a site directed mutagenesis method, plasmid vectors for Tim-3-Ig N-glycosylation mutant expression were produced. The recombinant protein was purified by protein A sepharose column chromatography. The binding activity of Tim-3-Ig fusion protein to $CD4^+CD25^+$T cells was analyzed using flow cytometry. Results: We found that the nonglycosylated Tim-3-Ig fusion proteins expressed in bacteria bound to $CD4^+CD25^+$T cells similarly to the glycosylated Tim-3-Ig protein produced in CHO cells. Further, three N-glycosylation mutant forms (N53Q, N100Q, N53/100Q) of Tim-3-Ig showed similar binding activities to those of wild type glycosylated Tim-3-Ig. Conclusion: Our results suggest that N-glycosylation of Tim-3 may not affect its binding activity to ligands expressed on $CD4^+CD25^+$T cells.

• Journal of Sericultural and Entomological Science
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• v.55 no.1
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• pp.1-5
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• 2019

This study was carried out to investigate the development and oviposition characteristics of Tenodera aridifolia. The nymphs of T. aridifolia grew to adults through 6 and 7 nymphal instars. The percentage of 7 nymphal instars (7-instar type) at 25, 28, and $30^{\circ}C$ were 77.3, 76.2, and 73.5%, respectively. In the 7 instars type, total nymphal periods at 25, 28, and $30^{\circ}C$ were 71.9, 53.5, and 54.7 days, respectively. And, there was no difference in deveolpmental periods between 6- and 7-instar types at all temperatures. Egg periods were 41.3 days at $25^{\circ}C$. Adult longevity of females were 82.0 days, deposited average 3.2 oothecae at intervals of 11.7~18.7 days at $28^{\circ}C$ during her life span. The first ootheca was the largest and $40.5mm{\times}22.0mm{\times}17.3mm$ in size. The number of average eggs per oothecae was 178.6 at $28^{\circ}C$.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.53-56
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• 2003

Development and reproduction of the rice armyworm, Pseudaletia separata Walker, were investigated under different temperatures (13, 15, 18, 20, 25, 30, and 33$^{\circ}C$ ). It took 80.6 days to grow from egg to pre-adult at 15$^{\circ}C$, and 27.3 days at 30$^{\circ}C$ (3.0 times shorter growth period compared with that at 30$^{\circ}C$ ). The range of developmental temperature of rice armywom was 15-30$^{\circ}C$. Survival rate from hatched larva to pre-adult was the highest as 70.6% at 25$^{\circ}C$. Pre-oviposition period and the adult longevity were 4.2 and 12.3 days at 15$^{\circ}C$, and 2.9 and 8.0 days at 30$^{\circ}C$, respectively. The highest average fecundity per female was 816.6 at 25$^{\circ}C$. The net reproductive rate (R$\_$o/) and intrinsic rate of natural increase (r$\_$m/) were the highest at 25$^{\circ}C$ as 913.0 and 0.175, respectively. As a result, it was considered that optimum of temperature for P. separata growth was = 25.0$^{\circ}C$.

• Journal of the Korea Organic Resources Recycling Association
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• v.6 no.1
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• pp.43-52
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• 1998

The objective of this study is to investigate the optimum conditions of extracting collagen without chrome ion from the leather waste. The effect of temperature, pH, and the concentration of alkaline solution on the collagen extraction has been studied. The result indicated that the incipient denatured temperature of collagen measured by viscosity was $25^{\circ}C$ and the complete denatured temperature was $31.5^{\circ}C$. The optimum solubilization condition for temperature was between $15^{\circ}C$ and $20^{\circ}C$, pH was 1.5, the concentration of alkaline solution was 3% of sodium hydroxide. The almost complete chrome ion separation was possible around the pH of 1.5. The separation efficiency of chrome ion from tannery waste was more than 99.5%. Extraction efficiency of crude protein from leather waste was about 89.5%. The hydroxyproline and collagen content in the extracted crude protein were 8.53% and 63.62%, respectively.

• Journal of the Korean Chemical Society
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• v.27 no.1
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• pp.66-72
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• 1983

Anionic polymerization of 2-pyrrolidone was carried out by TDI (Toluene Diisocyanate)/KOH catalysis. The effects of TDI / KOH mole ratio, KOH concentration, temperature and time on polymerization were investigated. It was observed that the highest rate of polymerization and maximum conversion were obtained when TDI / KOH mole ratio was about 0.25. The maximum conversion and the highest viscosity were obtained when the concentration of KOH was 5 mole percent. It was also found that the rate of polymerization and inherent viscosity at $30^{\circ}C$ were higher than those at $50^{\circ}C$. The rate constant ($k_p$) of polymerization was determined by least square method; the values of kp obtained were $57.53{\ell}/mole{\cdot}min\;at\;30^{\circ}C$ and $52.36{\ell}/mole{\cdot}min\;at\;50^{\circ}C$, respectively.