• Title/Summary/Keyword: 5-trichlorophenoxyacetic acid

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Isolation and Characterization of 2,4,5-Trichlorophenoxyacetic Acid Degrading Bacteria (2,4,5-Trichlorophenoxyacetic Acid 분해균주의 분리 및 특성)

  • Park, Yeong-Soon;Lee, Geon;Lee, Sang-Joon;Lee, Jong-Kun
    • Journal of Environmental Science International
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    • v.3 no.3
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    • pp.197-207
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    • 1994
  • Microorganisms capable of utilizing 2,4,5-trichlorophenoxyacetic acid(2,4,5-T) as sole carbon source were isolated from soil by enrichment culture. Among these strains, EL-O7IP had the highest biodegradability of 2,4,5-7, and according to its morphological and physiological characteristics, it was identified as Pseudomonas sp. This strain was resistant to rifampicin, streptomycin, ampicillin, kanamycin and such metal ions as $Zn^{2+}$, $Cu^{2+}$ Various compounds of chlorinated phenol and substrate analogs were more easily utilized than 2,4,5-7, but biodegradation rate for each compound was different. The strain easily utilized the compounds of chlorinated substituents on phenol in the order of ortho-, para-, and meta- position. The biodegradability of this strain was very stable. Key words : 2,4,5- trichlorophenoxyacetic acid, Pseudomonas sp .

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Studies on the synthesis of mannish bases of 2,2'-methylene bis(3,4,6-trichlorophenoxyacetic acid) and their antimicrobial activities (2,2'-methylene bis(3,4,6-trichlorophenoxyacetic acid)의 mannich bases합성및 항균작용에 관한 연구)

  • 김종호
    • YAKHAK HOEJI
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    • v.16 no.2
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    • pp.97-107
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    • 1972
  • Thirty-three Mannich bases of 2,2'-methylene bis(3,4,6-trichlorophenoxyacetic acid) were synthesized as potential antimicrobial agents and tested against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Trichophyton rubrum, Microsporum gypseum, Epidermophyton floccosum, Aspergillus niger and Aspergillus oryzae in vitro. It was found that: 1) Compounds 24 and 22 were active against Staphylococcus aureus and Bacillus subtilis at the concn. of 1 $\mu$g/ml respectively; 2) Compounds 9 and 29 were active against Trichophyton rubrum at the concn. of 2 $\mu$g/ml respectively; 3) Compouns 9 and 30 were active against Microsporum gypseum at the concn. of 2 $\mu$g/ml respectively; 4) Compounds 6,9,13,15,21,28,29,31,33 and 34 were active against Epidermophyton floccosum at the concn. of 1 $\mu$g/ml respectively; 5) Compounds 6,9,18 and 28 were active against Aspergillus niger and Aspergillus oryzae at the concn. of 1 $\mu$g/ml respectively.

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Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons (염화 방향족 탄화수소 분해세균의 분리 및 특성)

  • 김종우;김치경;김영창;염재홍;이재구
    • Korean Journal of Microbiology
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    • v.25 no.2
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    • pp.122-128
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    • 1987
  • Several bacterial isolates capable of degrading 4-chlorobiphenyl or 2,4,5-trichlorophenoxyacetic acid were isolated from industrial wastes by the agar plate method and studied for their biodegradabilities of the hydrocarbons and some biochemical characteristics. The isolates DJ-12, DJ-26 and TP-1 were identified as Pseudomonas spp. and they could not degrade 2,4-dichlorophenoxyacetic acid. The absorption spectra for 4-chlorobiphenyl and 2,4,5-trichlorophenoxyacetic acid showed the peaks at 253 and 292 nm, respectively. Biodegradability of the isolates was determined by decrease of the absorbance for the test hydrocarbons with a UV-scanning spectrophotometer. The plasmids of the isolates were studied to examine whether or not the hydrocarbon-degrading genes exist in the plasmids. Antibiotics resistance was also examined to search out a proper marker for the isolates in further experiments, such as curing test and genetic recombination.

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Isolation of 2,4,5-Trichlorophenoxyacetic Acid-Degrading Bacteria (2,4,5-trichlorophenoxyacetic acid 를 분해하는 세균의 분리)

  • Park, Young-Doo;Eum, Jin-Seong
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.1
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    • pp.47-51
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    • 2000
  • 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-degrading bacterial strains were isolated from rice field and field in suburbs of Taejon. Of the total 100 isolates, 19 strains were selected by fast growth on solid minimal media containing 2,4,5-T as a sole of carbon and energy, and they were identified to genus level. 11 strains were identified as Pseudomonas, 4 strains as Acinetobacter, 1 strains were as Alcaliagenes and 3 strains were not identified. Strains MU19 and MU92 which were identified as Pseudomonas were capable of degradation for 4 kinds of chlorinated aromatic hydrocarbons, 2,4-D, 2,4,5-T, MCPA and 3CB. Acinetobacter sp. MU38 showed the highest degradability in liquid minimal media at 48 hours after inoculation, and Pseudomonas spp. MU19. MU57, MU73, and MU92 were able to degrade carbon source at higher rates. As the results Acinetobacter sp. MU38 and Pseudomonas spp. MU19 and MU92 were capable of biodegradation for broad range of halogenated aromatic hydrocarbons, and had higher rates of degradation for 2,4,5-T.

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Effect of Acanthopanax extract on the DNA and erythrocyte damage induced by herbicides (제초제로 인한 DNA와 적혈구 손상에 미치는 오가피 추출물의 효과)

  • Seo, Yoo-Na;Kim, Jum-Ji;Sung, Kwang-Soo;Lee, Mi-Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.12
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    • pp.4922-4927
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    • 2010
  • In order to investigate whether the ethanol extract of Acanthopanax sp. might inhibit herbicide-induced DNA damage and erythrocyte damage, the suppression of the oxidative DNA damage of lymphocyte and erythrocyte damage in the presence of the extract were evaluated by comet assay and hemolysis assay, respectively. Phenoxy herbicides, named 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) and bipyridyl herbicide paraquat induced oxidative DNA damages of lymphocytes. However, the oxidative DNA damage by 2,4-D, 2,4,5-T or paraquat was inhibited in vitro upon treating Acanthopanax extract. Moreover, the erythrocyte damage was also suppressed in vitro by Acanthopanax extract treatment.

Suppressive Effect of Aqueous Extract of Red-Ginseng on the Herbicide-induced DNA Damage and Hemolysis

  • Seo, Yoo-Na;Lee, Mi-Young
    • Journal of Applied Biological Chemistry
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    • v.53 no.4
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    • pp.202-206
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    • 2010
  • The effects of aqueous extracts of red ginseng on the damage of DNA and erythrocyte by herbicides were evaluated using comet assay and hemolysis assay. Notably, the oxidative DNA damage and erytbrocyte hemolysis by 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) were significantly suppressed by red ginseng treatment. Moreover, red ginseng could suppress significantly paraquat-induced oxidative DNA damage and hemolysis. These suppressive effects of red ginseng on the herbicide-induced damages might be due to the antioxidant components.

2, 4, 5-Trichlorophenoxyacetic Acid 분해균의 유전적 특성에 관한 연구

  • Yoon, So-Yeong;Son, Hong-Joo;Lee, Geon;Lee, Sang-Joon;Lee, Jong-Kun
    • Korean Journal of Microbiology
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    • v.30 no.4
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    • pp.260-264
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    • 1992
  • Pseudotnorju.c sp. EL-071P degrading 2.4.5-trichlorophe~~oxyi~cetaicci d (2.3.5-T) was resistantto antibiotics: rifampicin. ampicillin. kanamycin and metal ions : Zn" and Cu".The plasmitl related to the degradation of 2.4.5-'r and rifa~npicin resistance was isolatecifrom the strain. Its size was about 40 Kb. As result of transforming the plasmid intoEsch~rirhiti coli MClOhl, it was confirmed that the plasmid ura.; related to 2.4.5-T degradation.The strain coulil grow in the various chlorinated aromatic analogs as the solc carbon source.In the case of chlorophcnols. the chlorinated mono-substituteti phenols were easily dcgradetlin the order ol' ortho-. ~ ~ a r um- ,c ~tu-position.T he 2.3.5-T mctaholism was inhibited by 4-chlorophenol of 2.4.5-7' analog. In non-chlorinateci aromatics. ~ C I I L O ~ I ~ Csa.l icylilte i~ndtoluene were uscd ax the carbon source by the strain and typestrain Acudonlotrtr.\ plrtirltrKCTC 1643 having clegrad;~bility of various aromatics. But naphtalene was usecl only bythe A~urlomonri.\ sp. EL-07 1 P.the A~urlomonri.\ sp. EL-07 1 P.

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