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http://dx.doi.org/10.5762/KAIS.2010.11.12.4922

Effect of Acanthopanax extract on the DNA and erythrocyte damage induced by herbicides  

Seo, Yoo-Na (Department of Medical Biotechnology, Soonchunhyang University)
Kim, Jum-Ji (Department of Medical Biotechnology, Soonchunhyang University)
Sung, Kwang-Soo (Sushin Ogapy Co., Ltd)
Lee, Mi-Young (Department of Medical Biotechnology, Soonchunhyang University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.11, no.12, 2010 , pp. 4922-4927 More about this Journal
Abstract
In order to investigate whether the ethanol extract of Acanthopanax sp. might inhibit herbicide-induced DNA damage and erythrocyte damage, the suppression of the oxidative DNA damage of lymphocyte and erythrocyte damage in the presence of the extract were evaluated by comet assay and hemolysis assay, respectively. Phenoxy herbicides, named 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) and bipyridyl herbicide paraquat induced oxidative DNA damages of lymphocytes. However, the oxidative DNA damage by 2,4-D, 2,4,5-T or paraquat was inhibited in vitro upon treating Acanthopanax extract. Moreover, the erythrocyte damage was also suppressed in vitro by Acanthopanax extract treatment.
Keywords
Paraquat; 2,4-D; 2,4,5-T; Comet assay; Hemolysis; Acanthopanax;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 D. N. Bateman, "Pharmacological treatments of paraquat poisoning", Hum Toxicol, 6, pp. 57-62, 1987.   DOI
2 S. K. Park, J. J. Kim, Y. M. Jeon, M. Y. Lee, "Suppressive effects of Korean indigenous Acanthopanax divaritacus on the allergic inflammation", J Appl Biol Chem, 50, pp. 155-159, 2007.   과학기술학회마을
3 D. N. Tripathi, G. B. Jena, "Intervention of astaxanthin against cyclophosphamide-induced oxidative stress and DNA damage: A study in mice", Chem Biol Interact, 180, pp. 398-406, 2009   DOI
4 N. P. Singh, M T. McCoy, R. R. Tice, E. L. Schneider, "A simple technique for quantitation of low levels of DNA damage in individual cells", Exp Cell Res, 175, pp. 184-191, 1988.   DOI
5 E. J. Park, K. K. Ryoo, Y. B, Lee, J. K. Lee, M. Y. Lee, "Protective effect of electrolyzed reduced water on the paraquat-induced oxidative damage of human lymphocyte DNA", J Korean Soc Appl Biol Chem, 48, pp. 155-160, 2005.   과학기술학회마을
6 D. G. Sul, S. N. Oh, E. I. Lee, "The expression of DNA polymerase-$\beta$ and DNA damage in jurkat cells exposed to hydrogen peroxide under hyperbaric pressure", Mol Cell Toxicol, 4, pp. 66-71, 2008.   과학기술학회마을
7 T. Katsu, M. Kuroko, T. Morikawa, K. San chika, Y. Fujita, H. Yamamura, M. Uda, "Mechanism of membrane damage induced by the amphipathic peptides gramicidin S and melittin", Biochim Biophys Acta, 983, pp. 135-141, 1989.   DOI
8 H. Murakami, S, Tsushima, Y. Shishido, "Soil suppressiveness to clubroot disease of Chinese cabbage caused by Plasmodiophora brassica", Soil Biol Biochem, 32, pp. 1637-1642, 2000.   DOI
9 A. Hartwig, "Role of DNA repair in particle -and fiber-induced lung injury", Inhal Toxicol, 14, pp. 91-100, 2002.   DOI
10 R. P. Hebbel, A. Leung, N. Mohandas, "Oxidation-induced changes in microrheologic properties of the red blood cell membrane", Blood, 76, pp. 1015-1020, 1990.
11 M. A. Ibrahim, G. G. Bond, T. A. Burke, P. Cole, F. N. Dost, P. E. Enterline, M. Gough, R. S. Greenberg, W. E. Halperin, E. McConnell, I. C. Munro, J. A. Swenberg, S. H. Zahm, J. D. Graham,, "Weight of the evidence on the human carcinogenicity of 2,4-D", Environ Health Perspect, 96, pp. 213-222, 1991.
12 J. S. Bus, J. E. Gibson, "Paraquat: model for oxidant-initiated toxicity", Environ Health Perspect, 55, pp. 37-46, 1984.   DOI   ScienceOn
13 E. L. Maillet, R. F. Margolskee, B. Mosinger, "Phenoxy herbicides and fibrates potently inhibit the human chemosensory receptor subunit T1R3", J Med Chem, 52, pp. 6931-6935, 2009.   DOI
14 E. Lynge, "Cancer incidence in Danish phenoxy herbicide workers, 1947-1993," Environ Health Perspect, 106, pp. 638-688, 1998.
15 A. Schecter, L. Needham, M. Pavuk, J. Michalek, J. Colacino, J. Ryan, O. Päpke, L. Birnbaum, "Agent orange exposure, Vietnam war veterans, and the risk of prostate cancer", Cancer, 115, pp. 3369-3371, 2009.   DOI
16 W.Yang, Tiffany-Castiglioni, E ,"Paraquat-induced apoptosis in human neuroblastoma SH-SY5Y cells: involvement of p53 and mitochondria", J Toxicol Environ Health A. 71, pp. 289-299, 2008.   DOI
17 M. Comporti, "Three models of free radical-induced cell injury". Chem Biol Interact, 72, pp. 1-56, 1989.   DOI
18 E. Zacharias, E. Suntres, "Role of antioxidant in paraquat toxicity", Toxicology, 180, pp. 65-77, 2002.   DOI
19 B. E. Shan, Y. Yoshita, T. Sugiura, U. Yama shita, "Suppressive effects of Chinese medicinal herb, Acanthopanax gracilistylus, extract on human lymphocytes in vitro", Clin Exp Immunol, 118, pp. 41-48, 1999.   DOI
20 J. M. Yi, M. S. Kim, S. W. Seo, K. N. Lee, C. S. Yook, H. M. Kim, "Acanthopanax senticosus root inhibits mast cell-dependent anaphylaxis", Clin Chim Acta, 312, pp. 163-168, 2001.   DOI