• Molecules and Cells
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• v.41 no.9
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• pp.868-880
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• 2018

Pancreatic cancer (PC) is one of the most aggressive cancers presenting with high rates of invasion and metastasis, and unfavorable prognoses. The current study aims to investigate whether EZH2/miR-139-5p axis affects epithelial-mesenchymal transition (EMT) and lymph node metastasis (LNM) in PC, and the mechanism how EZH2 regulates miR-139-5p. Human PC and adjacent normal tissues were collected to determine expression of EZH2 and miR-139-5p, and their relationship with clinicopathological features of PC. Human PC cell line was selected, and treated with miR-139-5p mimics/inhibitors, EZH2 vector or shEZH2 in order to validate the regulation of EZH2-mediated miR-139-5p in PC cells. Dual-luciferase report gene assay and chromatin immunoprecipitation assay were employed to identify the relationship between miR-139-5p and EZH2. RT-qPCR and Western blot analysis were conducted to determine the expression of miR-139-5p, EZH2 and EMT-related markers and ZEB1/2. Tumor formation ability and in vitro cell activity were also analyzed. Highly-expressed EZH2 and poorly-expressed miR-139-5p were detected in PC tissues, and miR-139-5p and EZH2 expressions were associated with patients at Stage III/IV, with LNM and highly-differentiated tumors. EZH2 suppressed the expression of miR-139-5p through up-regulating Histone 3 Lysine 27 Trimethylation (H3K27me3). EMT, cell proliferation, migration and invasion were impeded, and tumor formation and LNM were reduced in PC cells transfected with miR-139-5p mimics and shEZH2. MiR-139-5p transcription is inhibited by EZH2 through up-regulating H3K27me3, thereby down-regulation of EZH2 and up-regulation of miR-139-5p impede EMT and LNM in PC. In addition, the EZH2/miR-139-5p axis presents as a promising therapeutic strategy for the treatment of PC.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.1
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• pp.90-95
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• 2011

A Kick Motor, KSLV-I second stage propulsion system, utilizes a flexible seal for pitch and yaw axis controls during combustion. A flexible seal consists of the alternate laminate of rubber and composite reinforcement between forward and aft rings. A Kick Motor nozzle is rotated by the shear deformation of rubber layers. Consequently, the development of rubber, which is appropriate to the usage condition of flexible seal, is very important. A tensile test, QLS test (shear modulus and failure shear stress), and aging test have been carried out to confirm the performance of rubber developed. Test results show that the shear modulus of rubber are 0.4310 ~ 0.4997MPa and the failure shear stress is more than 2.5MPa.

• Transactions of the Korean Society of Mechanical Engineers
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• v.11 no.5
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• pp.740-745
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• 1987

For the prediction of the crack growth initiation from a blunt notch or a precrack in a prestrained material under plane strain tension and small-scale yielding conditions, a microscopic fracture criterion is proposed in terms of the crack tip opening displacement(COD) needed for the attainment of fracture strain at a microstructural distance. Smooth blunting of a crack tip with an initial root radius is assumed, and strain distributions on the crack-line axis are calculated at each deformation stage until the distributions against an original distance normalized to the COD are insensitive to an initial root radius. This case of no initial-root-radius effect is taken as for a sharp crack tip, on which the criterion is applied to determine the characteristic length of material from a critical COD for a fatigue-precracked specimen. The predicted COD at the fracture initiation from a crack with an initial root radius or a prestraining shows reasonable agreement with experimental values.

• Journal of the Korean Society for Precision Engineering
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• v.31 no.5
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• pp.381-387
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• 2014

This paper introduces a system to machine micro-sized patterns effectively on surface based on micro-milling process using tools with simultaneous rotation and oscillation, oscillation micro milling. To review the effectiveness of proposed concept, we integrated a micro-spindle supported by active magnetic bearings with a precision 3-axis air bearing stage using double-wedge mechanism, and tested this oscillation milling. Two types of oscillation milling were tested, which are linear oscillation milling with a flat end mill and elliptical oscillation milling with a ball end mill with 0.3 mm of diameter. The spindle was rotating 110 krpm and workpiece was moving constant speed of 2~8 mm/sec during the oscillation milling. As the results, multiple oval shape dimples were generated in regular spacing, and the variation of elliptical motion made different shapes of patterns. The results showed that proposed oscillation milling can be successfully used for machining repeated micro-patterns.

• Journal of the Earthquake Engineering Society of Korea
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• v.25 no.5
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• pp.191-199
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• 2021

In seismic design, hollow section concrete columns offer advantages by reducing the weight and seismic mass compared to concrete section RC bridge columns. However, the flexure-shear behavior and spirals strain of hollow section concrete columns are not well-understood. Octagonal RC bridge columns of a small-scale model were tested under cyclic lateral load with constant axial load. The volumetric ratio of the transverse spiral hoop of all specimens is 0.00206. The test results showed that the structural performance of the hollow specimen, such as the initial crack pattern, initial stiffness, and diagonal crack pattern, was comparable to that of the solid specimen. However, the lateral strength and ultimate displacement of the hollow specimen noticeably decreased after the drift ratio of 3%. The columns showed flexure-shear failure at the final stage. Analytical and experimental investigations are presented in this study to understand a correlation confinement steel ratio with neutral axis and a correlation between the strain of spirals and the shear resistance capacity of steel in hollow and solid section concrete columns. Furthermore, shear strength components (V_c, V_s·, V_p) and concrete stress were investigated.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.3
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• pp.412-419
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• 2009

The purpose of this study was to provide the reference data evaluating the treatment effect of orthopedic appliances. The skeletal and dental growth increments were measured in 24 normal mixed dentition children (boys: 14, girls: 10) by three serial lateral cephalograms: initial mean age: 9${\pm}$1.3 years, mean observation period: 13${\pm}$1.3 months. Cephalometric changes were analysed. The results were as follows: 1. In boys, the maxilla showed forward and downward growth pattern and the mandible showed forward growth pattern (p<0.05). In girls, the maxilla and mandible showed forward and downward growth pattern(p<0.05). 2. Horizontal growth of both maxilla and mandible in girls was superior to those in boys (A point; girls: 2.39mm, boys: 1.26mm, with p<0.05), whereas vertical growth of both maxilla and mandible in boys was similar to those in girls. 3. The change in tooth axis showed labioversion of upper incisor (p<0.01) and comparatively stable lower incisor position.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.275-279
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• 2003

Excised cotyledons and embryo axises of zygotic embryos of Rhus vemicifera were cultured on Murashige and Skoog(MS) medium with various concentrations of 2,4-D. About 3-5% of explants produced callus. Embryogenic callus was preferentially induced from basal parts of embryo axis of zygotic embryos seeds when they were cultured without removal of seed coats. Somatic embryos were developed from embryogenic callus in growth regulator-free medium after 2-3 subcultures on medium with 1.0mg/L 2,4-D and these embryos were matured to cotyledonary stage. Plantlets with well-developed shoots and roots from embryos were obtained on $\frac{1}{4}$MS medium with GA$_{3}$. After acclimatization of plantlets on artificial soil, they were exposed to soil pots.

• Journal of Families and Better Life
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• v.19 no.5
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• pp.51-66
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• 2001

This study attempted to examine the changing patterns of marital love constructs among married men and women in Korea. It is based on our prior research that showed each of the 3 dimensions of marital love(intimacy, passion, and commitment derived from Stermberg’s Triangular Theory of Love) shaped U pattern as the marriage continued. We analyzed 1687 respondents’answers by principal axis factoring with contextual point of view including individual, familial, and socio-cultural development. The results are as follows. First, the love constructs of Korean men and women in their marriage show different qualitative patterns as the marriage continued. Second, intimacy is the most powerful indicator of love, coming out the first factor in all the stages of marital relationships. Third we can see marital stability from the locus of commitment with other components of love and it could be apply to marital educational program for promoting marital stability. Forth, passion is found separated behavioral from perceived dimension except the first marital stage. As conclusion, it need to analyze with family life cycle. birth and marriage cohort groups for examining closely the causes of this qualitative changes in marital relationships.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2007.06a
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• pp.503-504
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• 2007

$Bi_2Sr_2Ca_{n-1}Cu_nO_x$(n=0, 1, 2)superconducting thin films have been fabricated by atomic layer-by-layer deposition at an ultra low growth rate using IBS(Ion Beam Sputtering) method. During the deposition, 90 mol% ozone gas of typical pressure of $1{\sim}9{\times}10^{-5}$ Torr are supplied with ultraviolet light irradiation for oxidation. XRD and RHEED investigations reveal out that a buffer layer with some different compositions is formed at the early deposition stage of less than 10 units cell and then Bi-2201 oriented along the c-axis is grown.

• Korean Journal of Optics and Photonics
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• v.32 no.2
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• pp.49-54
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• 2021

In this paper, we design and construct the equipment to manufacture large-diameter optical fiber end caps, which are the core parts of high-power fiber lasers, and we fabricate large-diameter optical fiber end caps using the home-made equipment. This equipment consists of a CO2 laser as a fusion-splice heat source, a precision stage assembly for transferring the position of a large-diameter optical fiber and an end cap, and a vision system used for alignment when the fusion splice is interlocked with the stage assembly. The output of the laser source is interlocked with the stage assembly to control the output, and the equipment is manufactured to align the polarization axis of the large-diameter polarization-maintaining optical fiber with the vision system. Optical fiber end caps were manufactured by laser fusion splicing of a large-diameter polarization-maintaining optical fiber with a clad diameter of 400 ㎛ and an end cap of 10×5×2 ㎣ (W×D×H) using home-made equipment. Signal-light insertion loss, polarization extinction ratio, and beam quality M2 of the fabricated large-diameter optical fiber end caps were measured to be 0.6%, 16.7 dB, and 1.21, respectively.