Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Plant Regeneration through Somatic Embryogenesis from Embryogenic Callus of Lacquer Tree (Rhus vernicifera Stokes)

참옻나무(Rhus verniciflua)배발생캘러스로부터 체세포배발생에 의한 식물체 재분화

  • 김재훈 (마이크로프랜츠 부설연구소) ;
  • 이원석 (마이크로프랜츠 부설연구소) ;
  • 권기원 (충남대학교 농업생명과학대학) ;
  • 안준교 ((주)바이오피아) ;
  • 최용익 (중앙대학교 인삼산업연구센터)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Excised cotyledons and embryo axises of zygotic embryos of Rhus vemicifera were cultured on Murashige and Skoog(MS) medium with various concentrations of 2,4-D. About 3-5% of explants produced callus. Embryogenic callus was preferentially induced from basal parts of embryo axis of zygotic embryos seeds when they were cultured without removal of seed coats. Somatic embryos were developed from embryogenic callus in growth regulator-free medium after 2-3 subcultures on medium with 1.0mg/L 2,4-D and these embryos were matured to cotyledonary stage. Plantlets with well-developed shoots and roots from embryos were obtained on $\frac{1}{4}$MS medium with GA$_{3}$. After acclimatization of plantlets on artificial soil, they were exposed to soil pots.

Keywords

References

  1. Been CG, Kim BD (1997) Totipotential, morphologtcal, biochemical comparisons between nonembryogenic callus and embryogenic callus in water dropwort (Oenanthe stolonifera DC). Kor J Plant Tiss Cul 24: 167-173
  2. Ghoi YE, Kim JW, YoonES (1999) High frequency of plant production via somatic embryogenesis from callus or cell suspension cultures in Eleutherocoocus senticosus. Ann Bot 83: 309-314 https://doi.org/10.1006/anbo.1998.0827
  3. Epstein WL (1989) Topical prevention of poison ivy/oak dermatitis. Arch Dermatol 125: 199-501
  4. Hirasuna TJ, Petchanker L, Srinivasan V, Shuler ML (1996) Taxol production in suspension cultures of Taxus baccata. Plant Cell Tiss Org CuI 44: 95-102 https://doi.org/10.1007/BF00048185
  5. Jansen MAK, Booij H, Schel JHN, De Vries SC (1990) Calcium increases the yield of somatic embryos in carrot embryogenic: suspension cultures. Plant Cell Rep 9: 221-223 https://doi.org/10.1007/BF00232184
  6. Kalish RS (1990) The use of human T-lymphocyte clones to study T-cell function in allergic contact dermatitis to urushiol. J Invest Dermatol 90: 108-111
  7. Kim JW, Soh WY (1996) Plant regeneration through somatic embryogenesis from suspension cultures of Allium fistulosum L Plant Sci 114: 215-220 https://doi.org/10.1016/0168-9452(96)04330-0
  8. Kim MJ, Choi YH, Kim WG, Kwak SS (1997a) Antioxidative activity of urushiol derivatives from the sap of lacquer tree (Rhus vernicifera Stokes). Kor J Plant Res 10: 227-230
  9. Kim MJ, Kim CJ, Kwak SS (1997b) Antifungal activity of urushiol camponents in the sap of Korean lacquer tree (Rhus vernicifera Stokes). Kor J Plant Res 10: 231-234
  10. KimYW,Youn Y, Noh ER, Kim JC (1999) Somatic embryogenesis and plant regeneration from immature zygotic embryos of Japanese larch (Larix leptolepis). Rant Cell Tiss Org Cult 58: 93-97 https://doi.org/10.1023/A:1006318928684
  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol Plant 15:473-49 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  12. Na CS, Jung NC, Oh KI (1998) In vitro cytotoxic activity of urushiol in the sap of Rhus verniciflua Stokes. J Kor For Soc 87: 260-269
  13. Nomura K, Komamine A (1985) Identification and isolation of single cells that produce somatic embryos at a high frequency in a carrot suspension culture. Plant Physiol 79: 988-991 https://doi.org/10.1104/pp.79.4.988
  14. Parrott WA (1991) Auxin-stimulated somatic embryogenesis from immature cotyledons of whit clover. Plant Cell Rep 10: 17-21
  15. Wainwright H, Flegmann AW (1985) The micropropagation of gooseberry (Ribes uvacrispa L): I. Establishment in vitro. J Hortic Sci 60: 215-221 https://doi.org/10.1080/14620316.1985.11515621