• 생명과학회지
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• 제26권7호
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• pp.796-804
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• 2016

본 연구에서 와인발효의 최적 균주를 조사하였으며 최종 선발된 Saccharomyces sp. BCNU 6006의 발효 특성과 항산화 활성을 측정하였다. 먼저 효모는 막걸리, 과일 그리고 발효 식품에서 분리하였으며, 분리된 균주는 β-glucosidase, glycosidase와 protease 효소 활성과 에탄올 및 SO₂ 내성에 의해 선별하였고, 추가로 황화수소와 바이오제닉 아민을 생성하는 균주를 제외하여 총 9균주가 선별되었다, 한편 선별균주들은 계통적으로 K. sevazzii와 S. cerevisiae의 subcluster에 속하는 균주로 확인되었다. 최종 선발된 Saccharomyces sp. BCNU 6006균주의 흑마늘 와인 최적 발효조건은 26 brix, 28℃, 10일로 확인되었다. 발효가 완료된 흑마늘 와인의 성분은 에탄올 15.03%, 12 brix, pH 4.01로 측정되었고, 총 polyphenol, 총 flavonoid함량, tannin 그리고 5-HMF의 함량은 각각 3.85 mg/ml, 0.51 mg/ml, 5.90 mg/ml, 0.07 mg/ml으로 확인되었다. DPPH 라디칼 소거능, ABTS 라디칼 소거능 및 환원력은 각각 90.77%, 95.20% 그리고 1.261로 측정되었으며, 흑마늘 진액보다 낮았으나, Superoxide anion 라디칼 소거능은 94.42%로 흑마늘 진액보다 높았다. 효소활성, 발효특성 그리고 항산화 효능을 기초로 판단하면 Saccharomyces sp. BCNU 6006에 의해 제조된 흑마늘 와인은 산업적으로 잠재성이 높음이 확인되었다.

• Archives of Pharmacal Research
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• 제27권7호
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• pp.742-750
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• 2004

This paper for the first time reports the isolation of 5 compounds from Phellinus linteus. A sphingolipid (1) and two tyrosinase inhibitory compounds (2, 3) along with two carboxylic acids (4, 5), were isolated from the fruiting body of Phellinus linteus (Berk ＆ Curt) Aoshima. The structure of compound 1 was identified as 1-O-$\beta$-D-glucopyranosyl-(2S, 3R, 4E, 8E)-2-[(2R)-2-hydroxyhexadecanoylamino]-9-methyl-4,8-octadecadiene-1,3-diol, known as cerebroside B, based on spectroscopic methods such as 1D and 2D NMR as well as by acid hydrolysis. Compounds 2-5 were identified as protocatechualdehyde (2), 5-hydroxymethyl-2-furaldehyde (HMF) (3), succinic acid (4), and fumaric acid (5) based on the spectroscopic evidence. Compounds 2 and 3 inhibited the oxidation of L-tyrosine catalyzed by mushroom tyrosinase with an $IC_{50}$ of 0.40 and 90.8 $\mu\textrm{g}$/mL, respectively. The inhibitory kinetics, which were analyzed by the Lineweaver-Burk plots, were found to be competitive and noncompetitive inhibitors with a $K_{j}$ of 1.1 $\mu\textrm{m}$ and 1.4 mM, respectively.

• 한국식품영양과학회지
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• 제42권1호
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• pp.62-67
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• 2013

생리활성이 높은 지황을 식품소재로 간편하게 활용할 수 있도록 하기 위한 연구로, 생지황과 숙지황을 동결건조한 후 이화학적 특성과 항산화능 분석 및 관능검사를 실시하였다. 명도와 황색도는 숙지황 분말이 숙지황 분말보다 낮았고, 적색도는 숙지황 분말이 생지황 분말보다 높았다. 당도 및 환원당 함량은 숙지황 분말이 $30.3^{\circ}Brix$ 및 6.06%로 생지황분말에 비하여 높았다. pH는 숙지황 분말은 4.23으로 생지황 분말의 6.71보다 낮았으며 산도는 숙지황 분말은 0.35%로 생지황 분말의 0.20%보다 높았다. 일반성분 분석에서 수분함량은 숙지황 분말 4.2%, 지황분말 3.8%로 숙지황 분말에서 높았으나 그 외 일반성분은 유사하였다. 유효지표성분 분석에서 catalpol 함량은 생지황 분말에서 144.96 mg/mL로 검출되었고 숙지황 분말에서는 47.20 mg/mL로 검출되었다. 5-HMF는 숙지황 분말에서만 검출되었다. 총 phenol 함량 측정 결과 생지황 분말이 2.09 mg/mL, 숙지황 분말이 3.66 mg/mL로 생지황 분말보다 숙지황 분말에서 현저히 높은 함량을 나타내었고 DPPH radical 소거능 측정 결과 $IC_{50}$ 값이 생지황 분말 26.64 mg/mL, 숙지황 분말 13.74 mg/mL로 숙지황 분말의 항산화능이 매우 높았다. Hydroxyl radical 소거능 $IC_{50}$ 값은 숙지황 분말의 값이 6.37 mg/mL로 생지황 분말의 13.27 mg/mL보다 더 낮게 나타나 숙지황 분말의 항산화능이 더 높게 나타났다. FRAP 활성 측정 결과 생지황 분말이 0.51 mg/mL, 숙지황 분말이 1.99 mg/mL로 숙지황 분말이 높아 전반적으로 숙지황 분말의 항산화능이 더 높았다. 이상의 결과로부터 숙지황 분말이 식품소재로 더 적당한 것으로 사료된다.

• Toxicological Research
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• 제21권1호
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• pp.63-70
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• 2005

Gami-Honghwa-Tang (KH-19), a herbal prescription for reducing the side effect of radiotherapy, is composed of eight crude herbs such as Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos, and Glycyrrhizae Radix. In this study, marker substances in KH-19 were analyzed by high performance liquid chromatography-diode array detector (HPLC-DAD) and safety evaluation of standardized KH-19 was evaluated by good laboratory practices (GLP) guideline of Korea Food and Drug Administration. HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5-hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, and decursin in the KH-19. In acute oral toxicity study on rat, transient inhibition of body weight was shown, but change in general symptom was not detected. No dead animal was observed up to 5,000 mg/kg in both male and female animals. In acute oral toxicity study on Beagle dog, transient vomiting, diarrhea, anorexia, and body weight reduction were observed, However, no dead animal was observed up to 2,000 mg/kg in both male and female animals.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1245-1253
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• 2014

One-step sulfuric acid saccharification of the red alga Pterocladiella capillacea was optimized, and various detoxification methods (neutralization, overliming, and electrodialysis) of the acid hydrolysate were evaluated for fermentation with the thermotolerant yeast Kluyveromyces marxianus. A proximate composition analysis indicated that P. capillacea was rich in carbohydrates. A significant galactose recovery of $81.1{\pm}5%$ was also achieved under the conditions of a 12% (w/v) biomass load, 5% (v/v) sulfuric acid, $121^{\circ}C$, and hydrolysis for 30 min. Among the various detoxification methods, electrodialysis was identified as the most suitable for fermentable sugar recovery and organic acid removal (100% reduction of formic and levulinic acids), even though it failed to reduce the amount of the inhibitor 5-HMF. As a result, K. marxianus fermentation with the electrodialyzed acid hydrolysate of P. capillacea resulted in the best ethanol levels and fermentation efficiency.

• Natural Product Sciences
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• 제20권3호
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• pp.182-190
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• 2014

An ultra-performance liquid chromatography (UPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS) method was established for quantitative analysis of twelve components, allantoin (1), morroniside (2), 5-hydroxymethyl-2-furfural (5-HMF) (3), loganin (4), coumarin (5), cinnamic acid (6), mesaconitine (7), cinnamaldehyde (8), hypaconitine (9), aconitine (10), alisol B (11), and alisol B acetate (12) in a Palmijihwang-hwan decoction. The twelve constituents were separated on a UPLC BEH C18 column ($2.1{\times}100mm$, $1.7{\mu}m$) at a column temperature of $40^{\circ}C$ by gradient elution with 0.1% (v/v) formic acid in water and acetonitrile as the mobile phase. The flow rate was 0.3 mL/min and the injection volume was $2.0{\mu}L$. Calibration curves of all compounds were acquired with values of the correlation coefficient ${\geq}0.99$ within the test ranges. The limits of detection and quantification for all analytes were 0.01 - 4.53 ng/mL and 0.03 - 13.60 ng/mL, respectively. The concentrations of the compounds 1 - 9 and 12 were 72.83, 4389.00, 4859.00, 3155.17, 223.67, 33.50, 1.97, 518.00, 2.25, and $25.00{\mu}g/g$, respectively. However, compounds 10 and 11 were not detected.

• 한국식품영양학회지
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• 제31권2호
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• pp.258-263
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• 2018

To assess a potential possibility of Deoduck as functional food resources, this study was performed to determine the changes in chemical components and antioxidant activities on Deoduck with various aging conditions; aging temperatures were 60, 70, and $80^{\circ}C$, and aging periods were 5, 10, 15, 30, and 50 days. We determined pH, total acidity, browning index, 5-hydroxymethyl-furfural, total phenolic contents, DPPH and ABTS radical scavenging activities of aged Deoduck. Total acidity of aged samples increased during aging treatment, at higher temperature and longer time. The pH value of aged Deoduck ranged from 4.97 to 3.76. Aged Deoduck at $60^{\circ}C$ decreased slowly than 70 and $80^{\circ}C$, and these results were similar in total acidity. 5-HMF and total phenolic contents increased when increased aging temperature and periods. The DPPH and ABTS radical scavenging activities of Deoduck were ranged from 0.374 to 1.560 mg TEAC eq/g and from 0.302 to 1.745 mg trolox eq/g, respectively.

• 혜화의학회지
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• 제9권2호
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• pp.173-182
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• 2001

1. $\ll$Graphical Materia Medica$\gg$ is first book that writes about Rehmanniae Radix Preparat. $\ll$Shen nong's herbal$\gg$ is only written about Rehmanniae Radix. 2. After the $\ll$Decoction Materia Medica$\gg$, orintal dortors use Rehmanniae Radix's effect is removing heat from the blood and Rehmanniae Radix's effect is enriching the blood. 3. Rehmanniae Radix Preparat is kinds of durgs for enriching the blood, because Rehmanniae Radix Preparat's effect do nourishing yin and supplementing blood. Therefore, Rehmanniae Radix Preparat is uesd simple recipe or Rehmanniae Radix Preparat is used with another herbs that shows so good effect of hematogenous functions at laboratory work. 4. Bone marrow is doing hematogenesis. That has relation to Rehmanniae Radix Preparat's effect is replenishing bone marrow, it's channel tropisms are liver kidney channels. 5. Rehmanniae Radix Preparat's guide component is 5-HMF, but effective component has relation with hematogenesis is mannitol of saccharides. And we need more reserch about Rehmanniae Radix Preparat.

• 한국식품영양과학회지
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• 제14권2호
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• pp.117-122
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• 1985

자두넥타의 가공(加工) 저장시(貯藏時) 색깔을 안정화(安定化) 할 수 있는 방법(方法)을 마련하기 위(爲)한 기초실험(基礎實驗)으로 자두넥타로부터 색소(色素)를 추출(抽出) 정제(精製)하여 그 구성성분(構成成分)으로 anthocyanin 0.62 mg/g fruit, total phenolics 4.69 mg/g fruit을 얻었고 이 색소(色素)는 525nm 부근(附近)에서 최대흡광도(最大吸光度)를 가졌다. 자두넥타의 퇴색(褪色)에 가장 중요(重要)한 영향인자(影響因子)가 되는 것은 온도(溫度)이며, 실온(室溫)에서 500 시간(時間)에 half life를 가지던 자두색소(色素)는 $80^{\circ}C$에서는 $20{\sim}30$시간(時間)으로 격감(激減)하였다. color loss는 pH 에도 크게 영향을 받았고 산도(酸度)가 증가(增加)함에 따라 색소(色素)의 안정도(安定度)가 증가(增加)하였다. Ascorbic acid, Cu 또는 Fe과 같은 금속이온의 첨가농도(添加濃度)가 증가(增加) 할수록 색소(色素)의 half life가 감소(減少)하며 열처리(熱處理)로 손상(損傷)된 자두넥타의 혼입량(混入量)이 증가(增加) 할수록 퇴색속도(褪色速度)가 증가(增加)하고 탄수화물(炭水化物), 분해대사물(分解代謝物)인 hydroxymethyl furfural의 함량(含量)이 증가(增加)될수록 색소손실율(色素損失率)이 증대(增大)되었다. 발효균주(醱酵菌株)의 배양(培養)에 따라 발효(醱酵)가 진행(進行)되는 동안 색소손실량(色素損失量)은 증가(增加)하였으며 추출색소(抽出色素)의 spectrum도 상이(相異)한 결과(結果)를 보여 주었다.

• 약학회지
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• 제55권2호
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• pp.121-126
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• 2011

Sipjundaebo-tang was a well-known restorative traditional herbal prescription that used to treat anemia, anorexia, fatigue and inflammation. In this study, we examined the bioconversion of compounds in the Sipjundaebo-tang (SJ) and fermented Sipjundaebo-tang with Lactobacillus fermentum KFRI 164 (FSJ) using established HPLC-DAD method. The chromatogram of FSJ has shown that the contents of six bioactive compounds 5-HMF, paeoniflorin, ferulic acid, cinnam aldehyde, decursin, glycyrrhizin in SJ has decreased. And the contents of unknown compounds (1), (2), (3), (4) and (5) in FSJ were higher than each contents of SJ. The antioxidant activities of SJ and FSJ were conducted by DPPH free radical and Hydrogen peroxide ($H_2O_2$) scavenging assay. Electron donating activity (EDA, %) value of FSJ has shown higher than 21.9% and 14.5% at a concentration of 0.5 mg/ml for DPPH radical scavenging activity and $H_2O_2$ scavenging activity, respectively. Also, the neuroprotective activities of SJ and FSJ against glutamate-induced oxidative stress in a mouse hippocampal cell line (HT22) were evaluated by MTT assay. As a result, FSJ has shown higher neuroprotective activity than 56.5% comparing with SJ. In conclusion, the fermented SJ using microorganism could convert compounds in SJ and enhance antioxidant activity and neuroprotective activity.