• Title/Summary/Keyword: 5'-Modification

검색결과 2,294건 처리시간 0.031초

Bow hull-form optimization in waves of a 66,000 DWT bulk carrier

  • Yu, Jin-Won;Lee, Cheol-Min;Lee, Inwon;Choi, Jung-Eun
    • International Journal of Naval Architecture and Ocean Engineering
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    • 제9권5호
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    • pp.499-508
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    • 2017
  • This paper uses optimization techniques to obtain bow hull form of a 66,000 DWT bulk carrier in calm water and in waves. Parametric modification functions of SAC and section shape of DLWL are used for hull form variation. Multi-objective functions are applied to minimize the wave-making resistance in calm water and added resistance in regular head wave of ${\lambda}/L=0.5$. WAVIS version 1.3 is used to obtain wave-making resistance. The modified Fujii and Takahashi's formula is applied to obtain the added resistance in short wave. The PSO algorithm is employed for the optimization technique. The resistance and motion characteristics in calm water and regular and irregular head waves of the three hull forms are compared. It has been shown that the optimal brings 13.2% reduction in the wave-making resistance and 13.8% reduction in the added resistance at ${\lambda}/L=0.5$; and the mean added resistance reduces by 9.5% at sea state 5.

유방암 환자의 식행동 수정을 위한 영양중재 효과판정 (Nutritional Intervention for Dietary Behavior Modification in Breast Cancer Patients)

  • 이경민;박정순;김형미;강순아
    • 한국산학기술학회논문지
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    • 제19권5호
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    • pp.444-450
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    • 2018
  • 본 연구는 유방암 환자의 식행동 수정을 위한 영양중재 효과판정을 위해 전문가 설문을 통한 통계분석으로 진행하였다. 연구의 대상은 2015년 12월부터 2016년 5월까지 강남세브란스병원에서 유방절제수술을 받은 30명의 여성 환자였다. 연구방법은 숙련된 임상영양사가 영양중재 전후 식행동 수정 및 체중 변화를 측정하였고, 운동, 음주 및 건강보조식품 복용여부에 대해서도 조사하였다. 연구결과 본 연구대상자의 평균 연령은 $54.3{\pm}9.3$세이었고, 월평균 체중감소율은 $1.4{\pm}3.5%$이었다. 영양중재 전후 식행동 수정변화 점수는 3점 척도로 측정 시 $2.00{\pm}0.45$ 점에서 $2.76{\pm}0.18$ 점으로 유의하게 상승하는 결과를 보였다(p<0.001). 음주를 하는 환자의 비율은 20%에서 영양중재 후 전원 금주하는 효과를 보였고, 운동수행 비율도 53%에서 영양중재 후 97%로 증가하였다. 건강보조식품의 복용 비율은 40%에서 영양중재 후 20%로 감소하는 결과를 보였다. 이상의 결과를 고려해 볼 때, 유방암환자의 영양중재 후 식행동 수정은 추후 유방암의 재발을 방지하는데 매우 중요하다고 사료된다.

Identification of a Sequence Containing Methylated Cytidine in Corynebacterium glutamicum and Brevibacterium flavum Using Bisulfite DNA Derivatization and Sequencing

  • Jang, Ki-Hyo;Chambers, Paul J.;Britz, Margaret L.
    • Journal of Microbiology and Biotechnology
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    • 제11권5호
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    • pp.819-824
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    • 2001
  • The principal DNA modification systems of the amino-acid-producing bacteria Corynebacterium glutamicum AS019, Brevibacterium flavum BF4, and B. lactofermentum BL1 was investigated using two approaches; digestion of plasmid DNA isolated from these species TseI and Fnu4HI, and sequence analysis of the putative methyltransferase target sites following the derivatization of DNA using metabisulfite treatment. The C. glutamicum and B. flavum strains showed similar digestion patterns to the two enzymes, indicating that the target for cytidine methyltransferase recognizes 5'-GCSGC-3'(where S is either G or C). Mapping the methylated cytidine sites by bisulfite derivatization, followed by PCR amplification and sequencing, was only possible when the protocol included an additional step eliminating any underivatized DNA after PCR amplification, thereby indicating that the derivatization was not $100\%$ efficient. This may have been due to the high G0C content of this genus. It was confirmed that C. glutamicum AS019 and B. flavum BF4 methylated the cytidine in the $Gm^5CCGC$ sequences, yet there were no similar patterns of methylation in B. lactofermentum, which was consistent with the distinctive degradation pattern seen for the above enzymes. These findings demonstrate the successful application of a modified bisulfite derivatization method with the Corynebacterium species for determining methylation patterns, and showed that different species in the geneus contain distinctive restriction and modification systems.

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당뇨환자를 위한 웹 기반의 유헬스 자가영양관리 프로그램 개발 (Development of Web-based u-Health Self-nutrition Management Program for Diabetic Patients)

  • 안윤;배제헌;김희선
    • 대한지역사회영양학회지
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    • 제19권4호
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    • pp.372-385
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    • 2014
  • Objectives: The purpose of this study was to develop web-based self-nutrition management u-Health program for diabetic patients (DMDMG: Diabetes Mellitus Dietary Management Guide) for achieving systematic self-management of diet. Methods: The program consisted of five parts with different contents according to the results of needs assessment. Five major parts were 1) meal management part which contains calorie prescription, meals recording and dietary assessment, 2) prevention of disease part with information of diabetes and assessment of dietary behavior, 3) dietary behavior modification part with an education on dietary behavior modification plan and dietary behavior plan, 4) meal plan containing a training section for meal plan and self constructing part for meal planning by making tables, and 5) information about myself which composed with general and physical information. The system proposed in this study provides nutrients intake results right after input of diet intake, which is possible with simultaneous calculation of input data in the server with 3,495 food and 1,821 meal data base. The nutrients analysis program was evaluated with 26 diabetic patients with two-day 24 hr recall. Results: The differences of nutrients intakes between DMDMG and CANPRO 3.0 ranged from 13.5-16.5%, which was caused by the differences of databases of the two programs. The characteristics of DMDMG were; 1) it can provide an interactive tailored nutrition management, 2) it is a practical tool of diabetes nutritional management, 3) the program gives motivation for the dietary behavior modification. Conclusions: The effectiveness of whole program needs to be conducted, but the program was an innovative tool for self-management of nutrient intakes, diet behaviors, meal management and tailored nutrition education.