• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.587-594
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• 2000

At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1992.10a
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• pp.59-64
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• 1992

터빈 블레이드와 같이 회전하는 구조물의 파단은 공진 근처에서 진동이 발 생할 때에 이에 기인하는 피로에 의하여 발생한다. 그러므로 이와 같은 파단 을 피하기 위해서는 설계 단계에서 이론적인 계산에 의하여 구조물의 고유 진동수를 결정하는 것이 상당히 중요하다. 판이 회전을 받게 되면 원심력에 의하여 판의 강성이 증가하므로 고유진동수가 회전하지 않는 판의 고유진동 수보다는 상당히 증가하게 된다. 이에 대한 연구가 국내외에서 상당수 행하 여졌지만, 연구의 대부분이 회전의 영향을 고려하지 않은 정지판(stationary plate)에 대한 것이며 뢰전을 고려한 연구는 극히 제한되어 있다. 또한 회전 의 영향을 고려한 연구의 대부분이 해석 대상을 보로서 단순화 시켰고 해법 으로는 유한요소법과 Ritz법 등을 사용하였다. 이는 블레이드가 지니고 있는 기하학적인 형상과 진동 특성이 해석적인 방법으로 해결하는 데에는 상당한 어려움이 있기 때문이다. 실제적으로는 터빈 블레이드와 같은 회전체의 진동 특성이 설치각이나 비틀림각, 판의 형상비, 회전속도 등의 변화에 의하여 영 향을 받기 때문에 보와 같은 진동 거동을 보이기보다는 판이나 셀과 같은 진동 거동을 보이므로 보다 정확한 해석을 수행하기 위해서는 해석 대상을 판이나 셀로서 취급하는 것이 타당하다. 따라서 본 연구에서는 위와 같은 이 유 때문에 해석 대상을 등방성 사각판과 직교이방성 복합재료 사각판으로 선택하였으며, 구조물의 고유진동수에 영향을 미치는 다음과 같은 인자들을 해석에 고려하였다. 1. 회전속도 (rotational speed) 2. 설치각 (setting angle) 3. 허브의 반경 (hub radius) 4. 판의 형상비 (aspect ratio) 5. 적층순서 (stacking sequence)구조물에 대한 동적실험(dynamic test)을 통하여 단기간에 동적특성을 결정하고 SDM(structure dynamic modification)이나 FRS(force response simulation)를 수행하여 임의의 좌표 공간에 대한 진동수준을 해석적으로 예측할 뿐만 아니라 구조물의 진동제어 를 위한 동적인자를 변경시킬 수 있는 정보를 제공하며 장비를 방진할 경우 신뢰성 있는 전달률을 결정할 수 있다. 실험적으로 철교, 교량이나 건물의 철골구조 및 2층 바닥 등 대,중형의 복잡한 구조물에 대항 동특성을 나타내 는 모빌리티를 결정할 경우 충격 가진 실험이 사용되는 실험장비 측면에서 나 실험을 수행하는 과정이 대체적으로 간편하다. 그러나 이 경우 대상 구조 물을 충분히 가진시킬수 있는 용량의 대형 충격기(large impact hammer)가 필요하게 된다. 이러한 동적실험은 약 길이 61m, 폭 16m의 4경간 교량에 대 하여 동적실험을 수행하여 가능성을 확인하였다. 여기서는 실험실 수준의 평 판모델을 제작하고 실제 현장에서 이루어질 수 있는 진동제어 구조물에 대 한 동적실험 및 FRS를 수행하는 과정과 동일하게 따름으로써 실제 발생할 수 있는 오차나 error를 실험실내의 차원에서 파악하여 진동원을 있는 구조 물에 대한 진동제어기술을 보유하고자 한다. 이용한 해마의 부피측정은 해마경화증 환자의 진단에 있어 육안적인 MR 진단이 어려운 제한된 경우에만 실제적 도움을 줄 수 있는 보조적인 방법으로 생각된다.ofile whereas relaxivity at high field is not affected by τS. On the other hand, the change in τV does not affect low field profile but stron

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.121-128
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• 1991

The degree of attraction of Toxoplasma gondii to vertebrate cells varies with cell type and cell phase. Human promyelocytic leukemia cells, HL-60, were synchronized by double thymidine block method and co-cultured with Toxoplasma for 1 hr at each cell stage to investigate the cell cycle specific susceptibility of parasites to host cells. For 30 hr the average number of Texoplasma that invaded was a little changed except at 3 hr from G1/S phase boundary which concurred with the peak point of DNA synthesis. At 3 hr which is a relatively short interval compared to whole S phase, modification of cells by parasitic invasion was most remarkable. The number of Toxoplasma that penetrated was increased to more than sin times. The shape of the cells became sludgy and almost indiscernible by strong accessibility of parasites only for an hour of mfd-S phase. The same auctuation was also observed at the second peak of S phase but weakly. This suggests that there be surface molecules concerning with the attachment of Texoplasma to the host cells, which is expressed at special point of S phase. further studies on the specific protein or similar molecules related could be carried out using synchronized HL-60 cells.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11a
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• pp.177-184
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• 2003

Numerical simulation on marine wind and sea surface elevation was carried out using both three-dimensional hydrostatic and non-hydrostatic models and a simple oceanic model from 0900 LST, August 13 to 0900 LST, August 15, 1995. As daytime easterly meso-scale sea-breeze from the eastern sea penetrates Kangnung city in the center part as basin and goes up along the slope of Mt. Taegullyang in the west, it confronts synoptic-scale westerly wind blowing over the top of the mountain at the mid of the eastern slope and then the resultant wind produces an upper level westerly return flow toward the East Sea. In a narrow band of weak surface wind within 10km of the coastal sea, wind stress is generally small, less than l${\times}$10E-2 Pa and it reaches 2 ${\times}$ 10E-2 Pa to the 35 km. Positive wind stress curl of 15 $\times$ 10E-5Pa $m^{-1}$ still exists in the same band and corresponds to the ascent of 70 em from the sea level. This is due to the generation of northerly wind driven current with a speed of 11 m $S^{-1}$ along the coast under the influence of south-easterly wind and makes an intrusion of warm waters from the southern sea into the northern coast, such as the East Korea Warm Current. On the other hand, even if nighttime downslope windstorm of 14m/s associated with both mountain wind and land-breeze produces the development of internal gravity waves with a hydraulic jump motion of air near the coastal inland surface, the surface wind in the coastal sea is relatively moderate south-westerly wind, resulting in moderate wind stress. Negative wind stress curl in the coast causes the subsidence of the sea surface of 15 em along the coast and south-westerly coastal surface wind drives alongshore south-easterly wind driven current, opposite to the daytime one. Then, it causes the intrusion of cold waters like the North Korea Cold Current in the northern coastal sea into the narrow band of the southern coastal sea. However, the band of positive wind stress curl at the distance of 30km away from the coast toward further offshore area can also cause the uprising of sea waters and the intrusion of warm waters from the southern sea toward the northern sea (northerly wind driven current), resulting in a counter-clockwise wind driven current. These clockwise and counter-clockwise currents much induce the formation of low clouds containing fog and drizzle in the coastal region.

• Journal of the HCI Society of Korea
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• v.1 no.1
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• pp.1-8
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• 2006

We suggest a graphical representation of the tension flow in tonal music using a piecewise parametric curve, which is a function of time illustrating the changing degree of tension in a corresponding chord progression. The tension curve can be edited by using conventional curve editing techniques to reharmonize the original music with reflecting the user's demand to control the tension of music. We introduce three different methods to measure the tension of a chord in terms of a specific key, which can be used to represent the tension of the chord numerically. Then, by interpolating the series of numerical tension values, a tension curve is constructed. In this paper, we show the tension curve editing method can be effectively used in several interesting applications: enhancing or weakening the overall feeling of tension in a whole song, the local control of tension in a specific region of music, the progressive transition of tension flow from source to target chord progressions, and natural connection of two songs with maintaining the smoothness of the tension flow. Our work shows the possibility of controlling the perceptual factor (tension) in music by using numerical methods. Most of the computations used in this paper are not expensive so they can be calculated in real time. We think that an interesting application of our method is an interactive modification of tension in background music according to the user's emotion or current scenario in the interactive environments such as games.

• Journal of the HCI Society of Korea
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• v.5 no.2
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• pp.15-24
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• 2010

A Digilog Book is an augmented reality (AR) based next generation publication supporting both sentimental analog emotions and digitized multi-sensory feedbacks by combining a conventional printed book and digital contents. As a Digilog Book authoring software, ARtalet provides an intuitive authoring environment through 3D user interface in AR environment. In this paper, we suggest ARtalet authoring environment based trajectory editing method to generate and manipulate a movement path of an augmented 3D object on the Digilog Book. Specifically, the translation points of the 3D manipulation prop is examined to determine that the point is a proper control point of a trajectory. Then the interpolation using splines is conducted to reconstruct the trajectory with smoothed form. The dynamic score based selection method is also exploited to effectively select small and dense control points of the trajectory. In an experimental evaluation our method took the same time and generated a similar amount of errors as the usual approach, but reduced the number of control points needed by over 90%. The reduced number of control points can properly reconstruct a movement path and drastically decrease the number of control point selections required for movement path modification. For control manipulation, the task completion time was reduced and there was less hand movement needed than with conventional method. Our method can be applicable to drawing or curve editing method in immersive In-Situ AR based education, game, design, animation, simulation application domains.

• The Journal of Korean Academy of Prosthodontics
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• v.20 no.1
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• pp.51-66
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• 1982

The purpose of this study was primarily to determine the relationship between temporomandibular joint mobility and generalized benign joint hypermobility. The subjects were 85 men and 76 women, who were students of dental and dental hygiene schools, aged 18 to 30 years old. They had no disturbances or complaints of movement of temporomandibular joints and other joints in the body. The joint mobility was measured by a test which is a modification of a method developed originally by Carter and Wilkinson (1964). The mandibular mobility was measured during active and passive maximal opening, laterotrusion, protrusion, and retrusion by Ingervall's method (1970). The obtained results were as follows: 1. The distribution of joint hypermobility disclosed was 4.8% in men and 19.7% in women, and 11.8% of total subjects. 2. The joint mobility index was a mean of 0.37 for men and 0.51 for women in total subjects, and 0.80 for men and 0.73 for women in hypermobile subjects. 3. The angle of passive dorsiflexion of the little finger was greater in the left than in the right hand for both sexes and in hypermobile subjects than in total subjects. 4. There was a positive correlation between the joint mobility index and the angle of passive dorsiflexion of the little finger in total subjects. 5. The joint mobility was greater in women than in men, and in the left than in the right hand. 6. In the active maximal mandibular movements of total subjects, the mean values for the opening capacity was 56.01 mm and 52.04mm, the laterotrusion mean 8.07 and 8.08, the protrusion mean 8.72 and 8.24, and the retrusion mean 0.48 and 0.49 for men and women respectively. 7. In the passive maximal mandibular movements of total subjects, the mean values for the opening capacity was 59.07mm and 54.85mm, the laterotrusion mean 8.90 and 9.12, the protrusion mean 10.03 and 10.00, and the retrusion mean 0.69 and 0.72 in men and women respectively. The active and passive maximal opening capacity was larger in men than in women but in the other movements there were no significant differences between men and women. 8. The range of active and passive maximal mandibular movements of hypermobile subjects tended to be larger in men but no significant difference in women compared with that of total subjects. 9. The range of maximal mandibular movements was increased more in passive than in active.

• 모자간호학회지
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• v.3 no.1
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• pp.38-48
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• 1993

This study was done to suggest modification in the present hospital policy for Labour and Delivery which separates women in labour from their families. The design for study was a descriptive study. The number of subjects consisted of 82 postpartum women who had delivered within 12 hours and for whom there were no complications either for the mothers or for the newborns. Data collection was done with a structured questionnaire which was analyzed using frequencies and percentiles. The results of this study are as follows : 1. Most of subjects (90.2%) wanted their family to participate in the labour process. Husband were chosen as the primary participant(79.3%). The number of subjects who wanted their family to support them through the whole labour process (35.4%) was the highest, followed by, during labour(32.9%), as contrasted to, during delivery (1.2%). 2. Support from husband during pregnancy ranged from 55.6%-87.6% for all items. Primary support was from husband during the pregnancy. 3. The subjects wanted support from their families in labour by way of encouraging(84.1%), consolidating(81.7%), changing position(43.9%), maintaining relaxation(35.4%) and helping with respiration control (29.3%). The results showed higher support on the emotional level than the physical and informational level (26.8%). 4. The subjects answered that they expected that participation by their family during the labour process would have the effect of increasing security for the women in labour(79.3%), providing a better relationship between the family and the women in labour (57.3%) and the newborns(34.1%), and making for an easier labour courses (23.2%). 5. The priority of family members that the subjects wanted support from was, the husband, mother and mother-in-law in that order. It can be concluded that nurses help to maintain a management policy in the maternity setting which ignores the needs of the women in labour. If consideration is to be given to these subjects, future programs should include participation by the family in the labour process.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.811-815
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• 2006

The objective of this study was to evaluate the effect of electric fan installation for milk production improvement of dairy cattle in Thailand. The study was conducted using 2 small-holder dairy farms in Chiang Mai province, during April to August 2004. Electric fans were installed in front of each row of cows. Each of the two rows of cows in the barn was defined as an experimental unit, thus each farm had two experimental units. The fans were operated alternately in 7-day intervals between rows of cows within each farm during the day or between 8.00 am to 8.00 pm. Non-operation periods were used as control. Milk yields were recorded. Data on environmental temperature and humidity were obtained from Chiang Mai Meteorological Center. Result from statistically analysis of milk record suggested an interaction between lactation period and fan installation. Therefore, this interaction term of lactation period and fan installation (PERIOD_FAN) was added as a variable to the regression model. Due to the repeated data collection of milk yield from the same cow (alternate week), milk yield was analyzed by repeated measure analysis (Mixed model). Least square means were calculated for all levels and used to compare between each pair-wise values. The final data were collected from the total of 18 cows with 2,072 data. Overall means and SEM of milk yields and days in milk separated into farm were $14.7{\pm}0.06kg/day$ and $176.3{\pm}2.2days$, and $15.2{\pm}0.22kg/day$ and $202.5{\pm}3.7$ days for farm A and farm B, respectively. For multivariable analysis, only PERIOD_FAN and humidity were significantly associated with milk yield. Only the first period of lactation showed that the amount of milk yields during fan installation was higher than that of non-fan installation (p<0.05). Cows with fan installation produced approximately 1.2 kg/cow more milk than cows without fan installation during this period. In conclusion, the use of electric fan operated during the day time increased milk production of cows during the first period of lactation.

• Journal of KIISE
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• v.42 no.4
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• pp.434-441
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• 2015

Next generation memory technologies, which we denote as 'new memory', have both non-volatile and byte addressable properties. These characteristics are expected to bring changes to the conventional computer system structure. In this paper, we propose a fast boot technique for hybrid main memory architectures that have both new memory and DRAM. The key technique used for fast booting is write-tracking. Write-tracking is used to detect and manage modified data detection and involves setting the kernel region to read-only. This setting is used to trigger intentional faults upon modification requests. As the fault handler can detect the faulting address, write-tracking makes use of the address to manage the modified data. In particular, in our case, we make use of the MMU (Memory Management Unit) translation table. When a write occurs to the boot completed state, write-tracking preserves the original state of the modified address of the kernel region to a particular location, and execution continues. Upon booting, the fast booting process restores the preserved data to the original kernel region allowing rapid system boot-up. We develop the fast booting technique in an actual embedded board equipped with new memory. The boot time is reduced to less than half a second compared to around 15 seconds that is required for the original system.