• Title/Summary/Keyword: 5'-Modification

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Development of healthy Han-sik nutrition education program featuring consumption of Korean foods for prevention of metabolic syndrome in Korean adults (한국 성인의 대사증후군 예방을 위한 건강한식 교육프로그램 개발)

  • Kang, Minji;Paik, Hee Young;Wie, Gyung-Ah;Joung, Hyojee
    • Journal of Nutrition and Health
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    • v.45 no.6
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    • pp.552-561
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    • 2012
  • Metabolic syndrome is a cluster of metabolic risk factors associated with increased risk of cardiovascular diseases. Recently, the prevalence of metabolic syndrome has increased in Korea. The aim of this study was to develop a Healthy Han-sik Nutrition Education Program (HHNEP) based on commonly recognized Korean foods in order to decrease metabolic syndrome risks. The target population of the HHNEP is adults with metabolic risk factors. The initial version of the HHNEP was developed based on a review of literature and various dietary guidelines by expert committees as well as the results of a survey on the perception of common Korean dishes and foods as Han-sik. The focus group discussion conducted of nine participants from the target population. After minor modification, the final version was developed. The contents included five sections: 1) aims, 2) introduction of Korean Food Guidance System, 3) cholesterol contents in food, 4) menu planning method using Han-sik, and 5) examples of daily Han-sik menu. The Han-sik list was selected based on the results from a Han-sik perception answered by 35 Korean adults aged 30-60 years using 517 frequently consumed Korean foods from the fourth Korea National Health and Nutrition Examination Survey. The nutrition education program was developed based on scientific evidence for the prevention of metabolic syndrome and focuses on tailoring education to an individual's dietary problems. Educational method was developed by a group of expert committees based in planned behavior theory and related research results. The education method consisted of assessment of current diet, four face-to-face nutrition education sessions over 8 weeks, and evaluation after 8 and 16 weeks. Initial version of nutrition education materials and methods was tested for feasibility by a select group of nine Korean healthy adults. Successful implementation of the program would include application by nutritional professionals at the health promotion center of the hospitals, public health center, and work sites. Intervention studies are needed to evaluate the feasibility and effectiveness of this program before large-scale applications.

문헌정보학과 WWW홈페이지의 필요성과 준비에 관한 연구- 한국과 북미주 지역 대학을 중심으로 -

  • 박일종
    • Journal of Korean Library and Information Science Society
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    • v.24
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    • pp.413-448
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    • 1996
  • Nowadays, the World Wide Web (WWW) has become an important resource of timely information for the information-related people such as information scientists, librarians, and students in Library and Information Sciences area. They are information professionals who navigate the information on the internet. Also, they need to be information providers who build a WWW homepage. This paper is a study of the necessity, preparation, and building WWW Homepage files for school of Library and Information Sciences in the age of competition among disciplines. It is particularly focused on the colleges and universities in Republic of Korea (ROK) and North America area. The purpose of this study is to provide various kinds of reference information to prepare a homepage in the future as utilizing information on the internet effectively. Even though a Web page was necessary for a school of Library and Information Sciences to show news, and introduce the purpose of the disciplines and the curriculum of the school, and the professors of a class etc., it was not well-prepared yet in ROK. However, a web page was used well enough and prosperous in North America area (Canada, the United States, and Puerto Rico) comparatively. Those web pages were analyzed and studied to prepare for a good designing of homepages for school of Library and Information Sciences in Korea and for the age of competition among disciplines in this paper. Suggestions for designing a good homepage and guidelines for preparing a best one were studied after both reviewing literature and utilizing experiences by the author who currently serves in the School of Library and Information Sciences in Keimyung University and builds homepage for the school. As a result, the major suggestions are ; premiered, and they are as follows: (1) English version of a homepage is necessary, (2) Provide a multimedia presentation about the nature of a school (3) Incorporate a place to let people make suggestions on the contents (of a homepage), (4) Bear in mind that potential users must be familiar with abbreviations you used, (5) Absolutely do not use abbreviations that may make the content more difficult to understand, (6) Add a feature on the every single page that will take the user back to the main page, (7) Use clear, short and well-structured sentences and remember to divide text into paragraphs, (8) Date with a creation or modification date in the homepage to indicate the updated date, (9) Being a multimedia environment, use colors effectively (the guidelines were also suggested), and (10) Put colleges' name into the title of bookmarks to find out them easily.

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Application of Micro-BLISA in Serodiagnosis of Fascioliasis in Cattle (간질증의 효소면역학적 진단)

  • Lee, Jae-Gu;Baek, Byeong-Geol;Lee, Sang-Bok
    • Parasites, Hosts and Diseases
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    • v.23 no.1
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    • pp.95-101
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    • 1985
  • Fascioliasis in cattle is one of the most common and very serious trematode diseases in Korea. In the present study, the enzyme linked immunosorbent assay (ELISA) was applied in the diagnosis of fascioliasis using antigen of Fasciela hepatica, perokidase of conjugate anti-cattle Is G and orthophenylenediamine as a substrate by micro-method technique of Volley et at. (1976b) and MacLaren (1978) with a slight modification. Results obtained from the present study are as follows. 1. In assay for optimal dilution of stock antigen, the antigen (protein contents; 0. Bmgymz) was diluted from 1150 to 1/600 with carbonate buffer (pH 9.6), and then absorbance values were measured with 1/100 diluted sera. The regression equations between the OD values of ELISA and dilution of antigen were log Y: -0.181-0.00127X in infected sera, and log Y: -0.578-0. 000879X in normal sera. The significantly higher (p<0.05) OD value was observed in the former. 2. In assay for optimal dilution of sera, the sera were diluted from 1125 to 1/400 with in PBSJ Tween 20 (pH 7.4), and absorbance values were measured with 1/200 diluted antigen. The regression equation between the OD values of ELISA and dilution of sera were log Y: -0.1540-0.0007238X in infected sera and log Y: -0.4834-0.00116X in normal sera. The former was higher than the latter (p<0.05). 3. In the 27 cases of negative intradermal test, OD values of the ELISA are $0.447{\pm}0.144$, the 95% confidence interval (Mean+2 H SD) of the values was 0.735, and there was no case over the values. Therefore, the sensitivity of the antigen to diagnose fascioliasis was 100% in the negative case. The OD value 0.7 which is designed as a criterion (detection level of positive one) is useful for the performance of the ELISA in fascioliasis. 4. According to the OD value of criterion in the regression equations, the optimal dilutions of stock antigen and serum were 1/250 and 1/100, respectively. 5. In the 58 cases of fascioliasis from which the adult could be found in the bile ducts, the OD value was $0.846{\pm}0.224$. The 75% (44 cattle) among them had higher value with compared to the criterion, and the 60% (20 cattle) of the cases of proliferative cholangitis of 33 cattle which had been infected previousely with Fasciola sp. is higher than the criterion. 6. Prevalence of fascioliasis was 43.4% in the application of the ELISA to 272 cattle which were reared in Jeonbug district.

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The Associated Factors with Obesity-related Habits and Obesity index Change (비만관련 습관 및 비만도 변화에 영향을 미치는 관련요인 분석)

  • Gil, Mi-Kyeong
    • Journal of the Korean Society of School Health
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    • v.12 no.1
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    • pp.27-44
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    • 1999
  • Obesity in children is a major concern of public health. This study was performed to illuminate its effect on weight control program and the associated factors of obesity-related habits and obesity index in primary school obese children. Weight control program consisted of behavioral modification, nutrition education and exercise during 17 weeks. The sample consisted of treatment group(n=42) and control group(n=41). There was no statistical difference between the two groups in obesity index, socioeconomic status and grade. To assess the effects of weight control program, the subjects were given pre-test and post-test such as the measurement of anthropometric values and self-reporting questionnaire. This result of this study were as follows; 1. After weight control program was applied, there was a significant decrease in obesity index among the treatment group. Obesity-related habits score of the treatment group increased significantly, While there was not much difference between the pre-test and the post-test among the control group. But exercise habit didn't increase significantly in the both groups. 2. Correlation between obesity-related habits and obesity index were not evident. 3. After application of weight control program, the factors associated with change of eating habit were children's past experience of weight control, motivational change toward weight control program and friends' support for treatment group. The factors associated with change of exercise habit were post-test motivation score and friends' support. Motivational change toward weight control and pre-test self-efficacy of exercise behavior were counter-related to exercise habit. For change of other obesity-related habits, initial obesity index, motivational change, post-test self-efficacy score of exercise behavior and paternal educational status were closely associated. But post-test self-efficacy score of eating behavior was unrelated. 4. Only the factor of experience of weight control was associated with change of obesity index. 5. For the both groups, the factors associated with change of eating habits were post-test self-efficacy of eating behavior and family's support. The factors associated with change of exercise behavior were self-efficacy changes of exercise behavior and friends' support. The factors associated with change of other obesity-related habits were self-efficacy change of eating behavior. Initial obesity index was associated with change of obesity index. 6. The rate of dropouts from weight control program was 28.6% (12/42) in treatment group. Initial obesity index, other obesity-related habits except eating exercise habits, friend's support were associated with dropout. In conclusion, these results indicated that weight control program in primary school settings was effective. Direct exercise regimen and practice was demanded. In addition to the program itself, much of the success is dependent on the degree of motivation of the children involved and support provided by their parents and friends. Further study need to be performed under the condition that the weight control progrom is applied for a longer period.

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Studies on the Operation Control with Automatic Silk Reeling Process to be responded for Korean Silk Cocoon (한국원료견질에 적응하는 자동조사 공정관리의 구명에 관한 연구)

  • 송기언
    • Journal of Sericultural and Entomological Science
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    • v.17 no.1
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    • pp.1-26
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    • 1975
  • These studies were attempted to find out the optimum silk reeling system by use of automatic silk reeling machine to increase raw silk yield and reeling efficiency with various silk reelable cocoons. The obtained results are as follows; 1. The mean silk reelability ratio(X)of the Korean cocoons during the last ten years was 61 per cent, beside 64.7 per cent in autumn cocoon and 57.3 per cent in spring cocoon. However, the ratio variation of autumn cocoons was larger than that of spring cocoons. 2. A positive correlation between cocoon filament breaks during its process and silk reelability levels was shown to be significant. The cocoons of both poor and good reelability evidenced "J" shape distribution on the filament break graph by the order of reeling cocoon end. Many bave breaks were found at the inner shell of the cocoons, or in case of poor reelability cocoons. 3. The morphology of broken cocoon ends during the process was classified into A, B, C, D, E and F types, The occurrence of B type was majority, but that of F type was minority among them. 4. In case of the cocoon cooking, H-type ion-exchanged soft water was better for good reelability cocoons, Na and H-type ion exchanged neutral soft water for those of fair reelability, and alkaline (Na-type) soft water for those of poor reelability, respectively. 5. The modification of cooking water by mixing the above different types of water (50% Na-type and 50% H-type passed by standard natural water; 75% Na-type and 25% H-type passed by hard natural water; 25% Na-type and 75% H-type passed by soluble natural water) made higher yield of raw silk with tess breaks of thread. 6, In case cocoon ends groping water included sodium hexametaphosphate as much as 800 ppm. the groping efficiency and raw silk yield of cocoon was improved. The effect was pronounced in case of poor reelability cordons. 7. The most reasonable cocoon cooking and silk reeling condition for automatic silk reeling process were observed to be rather incomplete cook with good reelability cocoons and optimum cook with poor reelability cocoons succeeded by the reeling bath temperature of 45$^{\circ}C$, 8. The reasonable silk reeling velocities were observed to be about 150m per min. for good reelability cocoons, 120m per min. for fair reelability ones and 90 to 120m per min. for poor reelability ones. 9. In order to improve the raw silk yield of cocoons and reeling efficiency, the cocoon stand-by-ratio for reeling should be kept at the level of 40 per cent for good reelability cocoons or at 60 per cent for poor reelability ones beside necessary end found cocoon condition.

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Adaptability Questions of O-D Table Estimation Models (기종점 통행표 산출모형의 적용성 평가)

  • 오상진;박병호
    • Journal of Korean Society of Transportation
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    • v.17 no.5
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    • pp.99-110
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    • 1999
  • This study deals with the adaptability questions of O-D table estimation models. Its objectives are two-fold; (1) to estimate the characteristics of various O-D table estimation models(i.e. linear regression models. entropy models and statistic models) and (2) to find the model which estimates the O-D table with the best accuracy under the various data conditions. In Pursuing the above, this study gives the particular attentions to the test of the models, using the Sioux Falls network and equilibrium assignment method of MINUTP. The major findings are the followings. Firstly. it finds that the statistic models have the most goodness of fat among all models, if the required data are all Prepared. But it Presents that statistic models are the most sensitive against the underspecification and inconsistency problems of link data. Secondly, It shows that the linear regression models have the worst goodness of fat among all models. But the linear regression models are the most insensitive to the underspecification and inconsistency problems. Thirdly, THE/1 model of entropy model is sensitive against the underspecification and incon-sistency problems, but THE/2 model is insensitive. Finally, other informations like total volume, zonal Production and attraction volumes in 0-D table, help models to gain the better goodness of fit. Especially, in the statistic models. both the zonal production and attraction volume data are helpful to estimate the link volumes. It can be expected that the results dive some implications not only to the selection of optimal model under the various given data, but also to the development or modification of model.

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Effects of Stream Modification in the Seomjin River on the Fish Communities of the Dongjin River in Korea (섬진강의 유로변갱이 동진강 어류군집에 미치는 영향)

  • KIM Ik-Soo;LEE Wan-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.6
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    • pp.549-556
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    • 1984
  • Forty-eight fish species belonging to 38 genera and 10 families were collected along the Dongjin River from July 1982 to May 1984. The dominant species of them were Rhinogobius brunneus and Zacco platypus in the main stream and Abbottina springeri, Hemiculter eigenmanni and Moroco oxycephalus in the tributaries respectively. It was remarkable point in the distributional study of freshwater-fishes of Korea that both species of Pelteobagrus nitidus and Lefua costata were observed in the present studied areas for the first time. It was found that 6 species of Hypomesus olidus, Hemiculter eigenmanni, Pseudobagrus sp., Liobagrus mediadiposalis, Cobitis taenia striata, and Lepomis macrochirus inhabiting the seomjin River were unstably incorporated into the fish communities at the upper reaches of the Dongjin River which the Seomjin River flow down for the water power generation. It is suggested that the integradation between two subspecies of C. taenia occurs at the intermediate water areas in the mainstream of the Dongjin River, whereas C. t. striate is remained only at the upper reaches and C. t. lutheri is lived in the lower parts of the river. Population of C. koreensis found in the mainstream was exhibited considerable differences in the color patterns on the body sides of them in comparison with other populations, which was discussed in relation to the habitat variations adapted to the confluent condition of joining the Seomjin stream with the Dongjin River.

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The Effect of L-Carnitine and Isoflavone Supplementation on Weight Reduction and Visceral Fat Accumulation in Overweight Women (과체중 여성에서 L-carnitine과 Isoflavone 보충 섭취가 체중 및 내장지방 감소에 미치는 영향)

  • Gwak, Jung-Hyun;Lee, Jong-Ho;Lee, Sang-Jun;Park, Hyun-Woo;Kim, Yoo;Hyun, Yae-Jung
    • Journal of Nutrition and Health
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    • v.40 no.7
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    • pp.630-638
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    • 2007
  • This study was performed to examine the combined effects of L-carnitine and isoflavone supplementation on weight reduction and body fat distribution in overweight women. Overweight/obese women (body mass index > $23kg/m^2$) who were not diagnosed any type of diseases were included in this study and sixty subjects ($41.1{\pm}1.5$ years, $25.9{\pm}0.3kg/m^2)$ were randomly assigned to a placebo (n=30) or a supplement group (n=30, L-carnitine 300 mg+isoflavone 40 mg/day). We measured anthropometric parameters, abdominal fat distribution by computerizd tomography and blood components before and after the 12 week intervention period. After the 12 weeks of supplementation, subjects in L-carnitine and isoflavone supplement group showed a significant reduction of body weight (p < 0.001), body fat % (p < 0.05), and waist to hip ratio (p < 0.01) whereas placebo group did not show any changes. In a CT-scanned results, total fat area at L4 level was significantly reduced by 8.1% (p < 0.01) with the reduction of visceral fat area (-11.1%, p < 0.001) and subcutaneous fat area (-7.0%, p < 0.05) in the supplement group. The supplementation of L-carnitine and isoflavone showed the significant improvement of HDL-C (p < 0.01) and apoB (p < 0.05) concentrations, however, change values in those markers were not significant compared with those of the placebo group. In addition, a significant increase of adiponectin level (p<0.001) was observed in the supplement group after the intervention. The result of present study demonstrated that supplementation of 300 mg L-carnitine and 40 mg isoflavone per day fur 12 weeks can give beneficial effects on weight reduction and visceral fat accumulation. These potential antiobesity supplement can produce more favorable effects when combined with lifestyle modification.

Polymorphisms and Functional Analysis of the Intact Human Papillomavirus16 E2 Gene

  • Ekalaksananan, Tipaya;Jungpol, Watcharapol;Prasitthimay, Chuthamas;Wongjampa, Weerayut;Kongyingyoes, Bunkerd;Pientong, Chamsai
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.23
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    • pp.10255-10262
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    • 2015
  • High risk human papillomavirus (HR-HPV) E2 proteins play roles in transcriptional regulation and are commonly functionally disrupted when the HPV genome integrates into host chromosomes. Some 15-40% of cancer cases, however, contain an intact E2 gene or episomal HPV. In these cases, polymorphism of the E2 gene might be involved. This study aimed to determine polymorphisms of the E2 gene in episomal HPV16 detected in high grade squamous intraepithelial lesions and squamous cell carcinomas and altered functions compared to the E2 prototype. The E2 gene was amplified and sequenced. Two expression vectors containing E2 gene polymorphisms were constructed and transfected in SiHa and C33A cells, then E6 gene as well as Il-10 and TNF-${\alpha}$ expression was determined by quantitative RT-PCR. Expression vectors and reporter vectors containing the HPV16 long control region (LCR) were co-transfected and transcriptional activity was determined. The results showed that a total of 32 nucleotides and 23 amino acids were changed in all 20 cases of study, found in the transactivation (TA) domain, hinge (H) region and DNA binding (DB) domain with 14, 5 and 13 nucleotide positions. They mostly caused amino acid change. The expressing vectors containing different E2 gene polymorphisms showed E6 mRNA suppression, TNF-${\alpha}$ mRNA suppression and IL-10 induction but no statistically significant differences when compared to the E2 prototype. Moreover, promoter activity in HPV16 LCR was not affected by E2 protein with different gene polymorphisms, in contrast to nucleotide variations in LCR that showed an effect on transcription activity. These results demonstrated that E2 gene polymorphisms of episomal HPV16 did not affect transcriptional regulation and suggested that nucleotide variation as well as epigenetic modification of the LCR might play a role in inducing malignant transformation of cells containing episomal HPV16.

Effect of Dietary Intake of Ultra-fine or Nano-Scale Pulverized Cornstarch on the Growing Performance and Gut Function in Rats (Nano-Scale Pulverizer (NSP)와 Ultra-Fine Pulverizer (UFP)로 물리적 변성된 옥수수전분 섭취가 흰쥐의 성장능력 및 장기능에 미치는 영향)

  • Lee, Hye-Sung;Ju, Da-Nim;Kim, Bo-Ram;Kim, Sun-Hee;Han, Myung-Ryun;Kim, Myung-Hwan;Chang, Moon-Jeong
    • Journal of Nutrition and Health
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    • v.42 no.8
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    • pp.740-749
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    • 2009
  • The objectives of this study was to determine whether a new physically modified cornstarch by ultra-fine- or nanoscale pulverizer to reduce particle size offers better bioactive function than native cornstarch in weanling Sprague-Dawley rats. Male weaning Sprague-Dawley rats were fed diets containing native cornstarch (NAC), ultra fine pulverized cornstarch (UFC) or nano-scale pulverized cornstarch (NSC) for 4 weeks. In vitro rate of starch hydrolysis, growth performance, organ weight, intestine length intestinal proliferation and the fermentation by Bifidobacterium of rat cecum were evaluated. The diet with reduced particle size (UFC or NSC) significantly increased body weight gain and organ weight. Feed efficiency was increased in NSC fed rats and was not affected in UFC fed rats. Intestinal proliferation was decreased in NSC group. Reduction of particle size also increased cecal short chain fatty acid concentration and the growth and acidifying activity of Bifidobacterium. It is concluded that a reduction of particle size of starch granules by physically modification may increase growing performance and gut function.