• Title/Summary/Keyword: 4P Analysis

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Molecular Cloning and Expression of Cellulase of Gene of Pseudomonas sp. in Escherichia coli (Pseudomonas sp.의 Cellulase 유전자의 대장균에의 클로닝 및 발현)

  • 정영철;김양우;노종수;성낙계;강신권
    • Microbiology and Biotechnology Letters
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    • v.18 no.6
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    • pp.633-639
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    • 1990
  • The genes for cellulases of Pseudomonas sp. LBC505 and CYC10, potent cellulase complex-producing strains, were cloned in Escherichia coli with pUC19. Recombinant plasmids pLCl and pLC2 were isolated from transformants producing cellulase by Congo red staining, and their genes cloned were 0.7 kb and 4.6 kb HindIII fragments, respectively. The inserts of pLCl and pLC2 were hybridized to chromosomal DNAs digested with HindIII from Pseudomona~ sp. LBC505 and CYC10, respectively. Immunodiffusion assays revealed that pLC1-and pLC2-encoded cellulase showed similarity with that of host strains. About 24% of cellulase activity was observed in the extracellular fraction of E. coli carrying pLC1, and its activity was higher about 1.4 times than that of LBC505. The enzymatic properties of pLC1 and pLC2 encoded cellulase were the same as those of cellulase from host strains. HPLC analysis and substrate specificity showed that cellulases were the same as those of cellulase from host strains. HPLC analysis and substrate specificity showed that cellulases cloned were endocellulase.

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Effect of pH on the Ester-crosslinking of Cotton Cellulose with PTCA and BTCA(I) (PTCA와 BTCA를 이용한 면셀룰로오스의 에스테르 가교화에 대한 pH 영향(I))

  • Chan-Min, Lee;Chul-Ho, Choi
    • Textile Coloration and Finishing
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    • v.9 no.5
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    • pp.30-41
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    • 1997
  • A purpose of this research is to prove unknown relation -ship between finish bath pH and crosslinking. In pursuit of these goals, we have treated 100% cotton broad cloth with PTCA and BTCA at different pH values. They were used with H$_{3}$PO$_{4}$, NaH$_{2}$PO$_{2}$, NaH$_{2}$PO$_{4}$, Na$_{2}$HPO$_{4}$, Na$_{3}$PO$_{4}$, catalysts to produce nonformaldehyde fabrics finishes. Treatments were applied to all cotton fabrics using a parletry-cure process. For the fully understood on the relationship of finish bath pH effect and cotton cellulose esterification, the relative concentrations of chemical species were calculated from ionization constants. The effect of pH on the cotton cellulose ester was investigated using Fourier transform infrared spectra, the surface area measurement by BET method and wrinkle recovery analysis. Results of differential FT-IR spectra and their relative concentration analysis were compared with those of catalyst treated controls. FT-IR and wrinkle recovery data indicated that the esterfication by polycarboxylic acids is pridependent. A similar phenomenon also occurred when a phosphate or hypophosphite was used. Therefore, it is necessary to choose the optimum pH range of a finishing bath in order to achieve the most effective esterification.

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Comparative analysis of cardiopulmonary resuscitation accuracy and fatigue by posture in hospitals (병원 내 심폐소생술의 자세별 피로도와 가슴압박 정확도 비교 분석)

  • Cho, Ki-Hwa;Yun, Jong-Geun
    • The Korean Journal of Emergency Medical Services
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    • v.25 no.3
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    • pp.179-188
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    • 2021
  • Purpose: This study aimed to identify effective cardiopulmonary resuscitation methods by comparing the fatigue of rescuers according to various positions in cardiopulmonary resuscitation situations conducted on beds in hospitals. Methods: An experimental study of students in the department of emergency medical service in H University, G Metropolitan City was conducted in four positions for applying chest pressure on mannequins on beds. Results: As a result of measuring the muscle fatigue of four muscle attachments according to the four positions conducted on the bed, the average was 3.4%, the P was significant at 0.001, and the fatigue difference was confirmed to occur depending on the attachment. An analysis of pressure depth by pose revealed that P1, P2, P3, and P4 have a depth of 58.3, 55.1, 56.4, and 56.3 mm, respectively, with P4 having the deepest depth. Conclusion: Among the various postures of the rescuer during cardiopulmonary resuscitation performed on the bed in the hospital, P1 is thought to be the most tiring, although its associated CPR quality is good.

Preparation and Termination of Carbosilane Dendrimer Based on Siloxane Tetramer (실옥산테트라머를 이용한 나뭇가지꼴 실란거대분자의 제조와 정지반응)

  • Kim, Chung Kyun;Park, Eun Mi
    • Journal of the Korean Chemical Society
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    • v.42 no.3
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    • pp.277-284
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    • 1998
  • Dendritic macromolecules of the first to fourth generation were synthesized, using alkenylation and hydrosilation cycles with allylmagnesium bromide and dichloromethylsilane as building blocks and siloxane tetramer $(Me(CH_2=CH)SiO)_4$, as core molecule. By the reaction of the dichloromethylsilyl-capped generation (G4P) with p-bromophenol, p-phenylphenol and lithium phenyethynylide, dendrimers with specific functions (G4P-BP (Mw: 16,300), G4P-PP (16,121), and CAP-PA (11,764)) have been produced. Analysis of new dendrimers by NMR, UV and MALD1 mass spectrometry suggests that they are pure and unified.

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A Study on CATV Home Shopping of Apparel and Life Style (소비자의 라이프스타일과 케이블TV 홈쇼핑 의류제품에 관한 연구)

  • 강영의;이옥희;최경은
    • The Research Journal of the Costume Culture
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    • v.9 no.3
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    • pp.343-356
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    • 2001
  • The purpose of this was to classify consumer groups according to the life styles and analyze buying intention and evaluation criteria of apparel product, opinion to 4p\`s aspect among consumer groups. And it was to clarify relationships between the experience of CATV home shopping and buying intention and evaluation criteria of apparel product, opinion to 4p\`s aspect. The subjects of this study were 270 women living in Chonbuk and Chonnam regardless their experience in CATV home shopping. For data analysis, frequency, percentage, mean, t-test, factor analysis were conducted. The results were as follows; First, the type of life style were classified into the following subdivisions: products confirmed type, price-quality regarded type, time regarded type, new product regarded type. Second, the significant differences among the classified life style groups were found in buying intention, evaluation criteria of apparel product, and opinion to 4p\`s aspect. Third, there were significant differences in buying intention, evaluation criteria of apparel product, and opinion to 4p\`s aspect according to TV home shopping experience.

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High Cytoplasmic Expression of the Orphan Nuclear Receptor NR4A2 Predicts Poor Survival in Nasopharyngeal Carcinoma

  • Wang, Jian;Yang, Jing;Li, Bin-Bin;He, Zhi-Wei
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.5
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    • pp.2805-2809
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    • 2013
  • Objective: This study aimed at investigating whether the orphan nuclear receptor NR4A2 is significantly associated with clinicopathologic features and overall survival of patients with nasopharyngeal carcinoma (NPC). Methods: Immunohistochemistry was performed to determine NR4A2 protein expression in 84 NPC tissues and 20 non-cancerous nasopharyngeal (NP) tissues. The prognostic significance of NR4A2 protein expression was evaluated using Cox proportional hazards regression models and Kaplan-Meier survival analysis. Results: We did not find a significant association between total NR4A2 expression and clinicopathological variables in 84 patients with NPC. However, we observed that high cytoplasmic expression of NR4A2 was significantly associated with tumor size (T classification) (P = 0.006), lymph node metastasis (N classification) (P = 0.002) and clinical stage (P = 0.017). Patients with higher cytoplasmic NR4A2 expression had a significantly lower survival rate than those with lower cytoplasmic NR4A2 expression (P = 0.004). Multivariate Cox regression analysis analysis suggested that the level of cytoplasmic NR4A2 expression was an independent prognostic indicator for overall survival of patients with NPC (P = 0.033). Conclusions: High cytoplasmic expression of NR4A2 is a potential unfavorable prognostic factor for patients with NPC.

Relationship between University students' Marriage View and Quality of Life

  • Kim, Jungae;Cho, Euiyoung
    • International Journal of Advanced Culture Technology
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    • v.8 no.4
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    • pp.129-136
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    • 2020
  • This study was a cross-sectional descriptive study that analyzed the effect of quality of life on marriage views in college students. The purpose of this study was to identify the relationship between quality of life and marriage view. The study targets were 195 male and female university students aged between 20 and 25. The data collection was done only by those who voluntarily agreed to the study using online questionnaires, and the data collection period was from October 1 to October 30, 2020. Data analysis was performed using SPSS 18.0 for frequency analysis, χ2 analysis, Pearson correction, t-test, and simple regression analysis. The result of the study that men had higher conservative marriage view (M=3.22, SD=.716) than women (M=2.21, SD=.903, p<0.01), women had higher active marriages (M=3.94, SD=.955) than men (M=3.41, SD=.933, p<0.01), and women had higher exclusive marriage views (M=3.94, SD=.955) than men (M=3.41, SD=.933, p<0.01). And people with high quality of life positively influenced romantic marriage view (R2=.029, p<0.05), but negatively affected passive marriage view (R2=.029, p<0.05) and conservative marriage view (R2=.034, p<0.05). Based on the results of this study, a program is needed to create a positive marriage view for the married age group, create an environment for married people to positively engage in social and economic activities, and propose a program for creating healthy family relationships.

CPT-based lateral displacement analysis using p-y method for offshore mono-piles in clays

  • Kim, Garam;Park, Donggyu;Kyung, Doohyun;Lee, Junhwan
    • Geomechanics and Engineering
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    • v.7 no.4
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    • pp.459-475
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    • 2014
  • In this study, a CPT-based p-y analysis method was proposed for the displacement analysis of laterally loaded piles. Key consideration was the continuous soil profiling capability of CPT and cone resistance profiles that do not require artificial assumption or simplification for input parameter selection. The focus is on the application into offshore mono-piles embedded in clays. The correlations of p-y function components to the effective cone resistance were proposed, which can fully utilize CPT measurements. A case example was selected from the literature and used to validate the proposed method. Various parametric studies were performed to examine the effectiveness of the proposed method and investigate the effect of property profile and its depth resolution on the p-y analysis. It was found that the calculation could be largely misleading if wrongly interpreted sub-layer condition or inappropriate resolution of input soil profile was involved in the analyses. It was also found that there is a significant influence depth that dominates overall load response of pile. The soil profile and properties within this depth range affect most significantly calculated load responses, confirming that the soil profile within this depth range should be identified in more detail.

The Traffic Analysis of P2P-based Storm Botnet using Honeynet (허니넷을 이용한 P2P 기반 Storm 봇넷의 트래픽 분석)

  • Han, Kyoung-Soo;Lim, Kwang-Hyuk;Im, Eul-Gyu
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.19 no.4
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    • pp.51-61
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    • 2009
  • Recently, the cyber-attacks using botnets are being increased, Because these attacks pursue the money, the criminal aspect is also being increased, There are spreading of spam mail, DDoS(Distributed Denial of Service) attacks, propagations of malicious codes and malwares, phishings. leaks of sensitive informations as cyber-attacks that used botnets. There are many studies about detection and mitigation techniques against centralized botnets, namely IRC and HITP botnets. However, P2P botnets are still in an early stage of their studies. In this paper, we analyzed the traffics of the Peacomm bot that is one of P2P-based storm bot by using honeynet which is utilized in active analysis of network attacks. As a result, we could see that the Peacomm bot sends a large number of UDP packets to the zombies in wide network through P2P. Furthermore, we could know that the Peacomm bot makes the scale of botnet maintained and extended through these results. We expect that these results are used as a basis of detection and mitigation techniques against P2P botnets.

Structure analysis, and magnetic study of a new Gd-metal-organic framework single crystal grown by the slow-evaporation method (증발법으로 합성된 신규 가돌리늄 금속-유기골격체의 단결정 구조 분석 및 자성학적 특성 연구)

  • Song, Jeong Hwa
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.32 no.5
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    • pp.199-204
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    • 2022
  • A new three-dimensional Gd-MOF, [Gd(p-XBP4)4(H2O)]·W(CN)8; (1; p-XBP4 = N,N'-p-phenylenedimethylenbis (pyridin-4-one)) has been synthesized by slow-evaporation and its crystal structure was characterized by single-crystal X-ray diffraction (SCXRD) analysis. For each GdIII ion, there are seven coordination sites, which are occupied by six oxygen atoms of six p-XBP4 ligands and one oxygen atom from the water molecule. The [W(CN)8]3- anion exists for charge balance with cationic framework. The GdII ions are interconnected by the p-XBP4 ligand to form the three-dimensional structure. Considering the magnetic property of lanthanide ions, magnetic studies of Gd-MOF were investigated by direct-current (DC) magnetic susceptibilities measurements.