• Title/Summary/Keyword: 4G spectrum

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Poly-$\beta$-Hydroxybutyrate Produced by Pink-Pigmented Facultative Methylotrophic Bacterium from Methanol (분홍색 통성 메탄올 자화세균이 생산하는 Poly-$\beta$-Hydroxybutyrate)

  • 송미연;이재호;이용현
    • Microbiology and Biotechnology Letters
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    • v.18 no.3
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    • pp.273-279
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    • 1990
  • For poly- $\beta$ -hydroxybutyrate (PHB) production, a pink-pigmented facultative methylotrophic bacterium (PPFM) P-10 was newly isolated from soils through methanol-enrichment culture. The optimal medium composition for cell growth was 1.0% (vlv) of methanol as carbon source and l.Og/l of ,TEX>$NH_4Cl$, equivalent to C/N ratio of 13.2 at pH 7.0 and $30^{\circ}C$. To investigate the optimal condition for YHB accumulation, two-stage culture technique was adopted; first stage for cell growth and second stage for accumulation of PHB providing unbalanced growth conditions. The optimal PHB accumulation was 1.0% (vIv) of methanol and 0.26gll of $NH_4Cl$, C/N of 50.8 at pH 6.0. To overcome methanol inhibition on cell growth, intermittent feeding fed-batch culture technique was employed, and the cell concentration as high as 14gll with 40% of PHB was achieved. The purified PHB was identified using IR and $1^H NMR$ as homopolymer of 8hydroxybutyric acid. The absorption spectrum of extracted pink colored microbial pigment was alsa investigated.

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Ab Initio Calculated Structures and Vibrational Spectra of 1,3-Diethoxy-p-tert-butylcalix[4]crown-5-ether Complexed with Potassium Cation

  • Choe, Jong-In
    • Bulletin of the Korean Chemical Society
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    • v.28 no.2
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    • pp.235-240
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    • 2007
  • Molecular structures were optimized for the 1,3-diethoxycalix[4]crown-5-ether (2) in the various isomers and their potassium-ion complexes by using B3LYP/6-31+G(d,p)//B1LYP/6-31G(d,p) method after ab initio RHF/6-31G calculation. The cone-shaped isomer of 2 with cr-binding mode has shown the strongest binding efficiency among the six different complexes attributed to seven electrostatic interactions between the potassium cation and the oxygen atoms of crown-5-ether and ethoxy groups of the host (2). The vibrational spectra of 2 and its K+-complexes were obtained by restricted Hartree-Fock (RHF) calculations with the 6-31G basis set. The characteristic vibrational frequencies of various C-O-C stretching and bending motions are analyzed.

HetNet Characteristics and Models in 5G Networks

  • Alotaibi, Sultan
    • International Journal of Computer Science & Network Security
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    • v.22 no.4
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    • pp.27-32
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    • 2022
  • The fifth generation (5G) mobile communication technology is designed to meet all communication needs. Heterogeneous networks (HetNets) are a new emerging network structure. HetNets have greater potential for radio resource reuse and better service quality than homogeneous networks since they can evolve small cells into macrocells. Effective resource allocation techniques reduce inter-user interference while optimizing the utilization of limited spectrum resources in HetNets. This article discusses resource allocation in 5G HetNets. This paper explains HetNets and how they work. Typical cell types in HetNets are summarized. Also, HetNets models are explained in the third section. The fourth component addresses radio resource control and mobility management. Moreover, future study in this subject may benefit from this article's significant insights on how HetNets function.

DNA Sequence Analysis of 1-Nitropyrene-4,5-Oxide and 1-Nitropyrene-9,10-Oxide Induced Mutations in the hprt Gene of Chinese Hamster Ovary Cells

  • Kim, Hyun-Jo;Kim, Tae-Ho;Lee, Sun-Young;Lee, Dong-Hoon;Kim, Sang-In;Pfeifer, Gerd P.;Kim, Seog K.;Lee, Chong-Soon
    • Molecules and Cells
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    • v.19 no.1
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    • pp.114-123
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    • 2005
  • Nitropyrene, the predominant nitropolycyclic hydrocarbon found in diesel exhaust, is a mutagenic and tumorigenic environmental pollutant that requires metabolic activation via nitroreduction and ring oxidation. In order to determine the role of ring oxidation in the mutagenicity of 1-nitropyrene, its oxidative metabolites, 1-nitropyrene 4,5-oxide and 1-nitropyrene 9,10-oxide, were synthesized and their mutation spectra were determined in the coding region of hprt gene of CHO cells by a PCR amplification of reverse-transcribed hprt mRNA, followed by a DNA sequence analysis. A comparison of the two metabolites for mutation frequencies showed that 1-nitropyrene 9,10-oxide was 2-times higher than 1-nitropyrene 4,5-oxide. The mutation spectrum for 1-nitropyrene 4,5-oxide was base substitutions (33/49), one base deletions (11/49) and exon deletions (5/49). In the case of 1-nitropyrene 9,10-oxide, base substitutions (27/50), one base deletions (15/50), and exon deletions (8/50) were observed. Base substitutions were distributed randomly throughout the hprt gene. The majority of the base substitutions in mutant from 1-nitropyrene 4,5-oxide treated cells were $A{\rightarrow}G$ transition (15/33) and $G{\rightarrow}A$ transition (8/33). The predominant base substitution, $A{\rightarrow}G$ transition (11/27) and $G{\rightarrow}A$ transition (8/27), were also observed in mutant from 1-nitropyrene 9,10-oxide treated cells. The mutation at the site of adenine and guanine was consistent with the previous results, where the sites of DNA adduct formed by these compounds were predominant at the sites of purines. A comparison of the mutational patterns between 1-nitropyrene 4,5-oxide and 1-nitropyrene 9,10-oxide showed that there were no significant differences in the overall mutational spectrum. These results indicate that each oxidative metabolite exhibits an equal contribution to the mutagenicity of 1-nitropyrene, and ring oxidation of 1-nitropyrene is an important metabolic pathway to the formation of significant lethal DNA lesions.

Systemic Manifestations of Immunoglobulin G4-Related Disease: A Pictorial Essay (다양한 인체 장기에서 보일 수 있는 면역글로불린 G4 관련 질환: 임상화보)

  • Kyungri Park;Yo Won Choi;Bo-Kyeong Kang;Ji Young Lee;Jeong Seon Park;Su-Jin Shin;Hye Ryoung Koo
    • Journal of the Korean Society of Radiology
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    • v.82 no.3
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    • pp.575-588
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    • 2021
  • Immunoglobulin G4 (IgG4)-related disease is a systemic fibro-inflammatory disease characterized by pathologic findings in various organs. Imaging is critical for the diagnosis and treatment assessment of patients with IgG4-related disease. In this pictorial essay, we review the key features of multiple imaging modalities, typical pathologic findings, and differential diagnosis of IgG4-related disease. This systematic pictorial review can further our understanding of the broad-spectrum manifestations of this disease.

Effects of Mixture and Systematic Application of Herbicides on Weed Control and Yield in Transplanted Rice (이앙답(移秧畓)에서 제초제(除草劑)의 혼합(混合), 조합처리(組合處理)가 제초효과(除草效果) 및 벼 수량(收量)에 미치는 영향)

  • Kim, J.K.;Ku, Y.C.;Lee, J.H.
    • Korean Journal of Weed Science
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    • v.2 no.1
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    • pp.20-30
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    • 1982
  • A field experiment was conducted in 1981 at the Crop Experiment Station, Suweon, Korea, in machine transplanted paddy rice field, to study the effectiveness of single herbicide, mixture, and systematic application of herbicides on diversity of weed control spectrum. The rice variety planted was Taebaegbyeo, Indica ${\times}$ Japonica cross bred. Experimental field was dominated by Echinochtoa crusgalli, Eleocharis kuroguwai, and Scirpus hotarui, and importance values based on dry weight of these weeds were 89%, 5%, and 3%, respectively. The mixture or systematic treatments of herbicide were generally more effective than single herbicide applications on weed control. Coefficients of similarity based on floristic composition after herbicide application between Perfluidone (5G) and Chloromethoxynil (7G), and between Pertluidone (5G) and Bifenox (7G), and between Perfluidone (5G) and three types of Butachlor (6G) were low, and these sets seemed to be a good mixture herbicide in paddy fields. While, Perfluidone (5G) had low coefficient of similarity with other single herbicides tested. The information on coefficient of similarity could be used as parameter for selecting herbicides to increase the efficiency of herbicidal performance. Simpson's indices from Butachlor (3.5G)/SL-49 (7G), Butachlor (3.5G)/Pyrazolate (6G), and Perfluidone (5G) treatments were high, and these herbicide treatments tended to the weed community type simplified, while the indices from Perfluidone (5G) + Chloromethoxynil (7G), Butachlor (6G) fb Perftuidone (5G), and Butachlor (4G)/Naproanilide (6G) treatments were low, and these herbicide treatments caused to the community type diversified in terms of floristic composition.

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Detection of Irradiated Milk Formulas using Electron Spin Resonance (전자스핀공명법(ESR)을 이용한 방사선조사 조제유류의 판별)

  • Woon, Jae-Ho;Park, Byeong-Ryong;Choi, Byung-Kook;Kim, Na-Young;Jeong, Hong-Jeom;Cheong, Ki-Soo;Kim, Hee-Sun;Kim, Chang-Seob
    • Journal of Dairy Science and Biotechnology
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    • v.28 no.2
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    • pp.13-18
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    • 2010
  • Electron Spin Resonance (ESR) spectroscopy has been used to detect the presence of radiation-induced free radicals in biological samples since the mid 1950s and to irradiate foods containing cellulose, crystalline sugar, and bone. Therefore, we analyzed the ESR spectrum of irradiated infant formula and its ingredients in this study. Samples were irradiated with 2 different radiation sources of $^{60}Co$ gamma rays and electron beams (EBs), and the absorbed doses were 0, 1, 3, 5, and 7 kGy. ESR measurements were performed under normal atmospheric conditions using a JEOL JES-FA100 spectrometer equipped with an X-band bridge. Irradiated infant formula showed anunsymmetrical spectrum ($g_1$=2.0050, $g_2$=2.0006); in contrast, non-irradiated samples showed asymmetrical spectrum. The ingredients of irradiated samples showed a multi-component ESR signal in glucose and lactose and a singlet-type spectrum in milk powder (g=2.0050). $R^2$ of the dose-response curve showed a fine linearity of over 0.95 across the entire sample. We also compared the spectra of identical samples irradiated with $^{60}Co$ gamma rays and EBs, because EBs can be used for food irradiation in foreign countries, although this is not permitted in Korea. However, we could not find any significant differences according to the types of radiation source. Thus, ESR spectroscopy can be used to detect irradiated infant formula and several types of primary ingredients in this formula.

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Gamma Radiation Induced Mutational Spectrum of Laccase Gene in Pleurotus ostreatus

  • Lee, Young-Keun;Chang, Hwa-Hyoung;Kim, Jae-Sung;Jang, Yu-Sin;Lee, Ho-Yong
    • Korean Journal of Environmental Biology
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    • v.20 no.4
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    • pp.316-324
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    • 2002
  • To investigate the mutational spectrum of laccase (Lac) genes (lac -A and lac -B) involved in degrading lignin which is the recalcitrant cell wall polymer, the genes of the Pleurotus ostreatus mutants induced by gamma ray radiation were amplified by PCR and were cloned. All partial lac-A genes of 4 mutants (PO-6, -7, -14 and -15) consisted of 1763 base pairs due to the deletion of two bases (491-nt and 492-nt) and addition of one base (875-nt) in 1764 base pairs of lac -A gene of PO-1. Totally 36 mutational hot spots were detected and 32 positions were mutated in all of those 4 mutants simultaneously. These mutations were predominantly A : T -> G : C transitions (40%). Putative amino acid sequences of lac -A genes of mutants have one simultaneous mutated residue (from Thr-44 to Ala-44). The 1764 bp of partial lac -B gene was cloned only in PO -5 mutant and contained 19 mutated bases. These mutations were predominantly G : C->A : T transitions (45%). Lac-B protein of PO-5 has two mutated residues of Glu-290 and His-363 from Ala-290 and Phe-363, respectively. The hyper-mutational positions were concentrated in specific regions of between 50-nt and 900-nt in lac genes. These results suggested that the mutational hotspots responded to gamma radiation could be in some genes, at least lac -A and lac -B of p. ostreatus.

Carotenoid Contents of Yellow Sweet Potatoes (황색고구마의 Carotenoid 색소 함량)

  • Kim, Seon-Jae;Rhim, Jong-Whan;Jung, Soon-Teck;Ahn, Young-Sup;Oh, Yong-Bee
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.218-222
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    • 1997
  • Twelve varieties of yellow sweet potatoes were measured for carotenoids content to assess their potential as a source of natural food colorant. Benihayato variety had the highest content of carotenoids (20.2 mg/100 g fr wt) but other varieties ranged with $4.6{\sim}16.7\;mg/100\;g$ fr wt. Hunter's +a-values (redness) increased linearly $(R^{2}=0.826)$ with carotenoids content of yellow sweet potatoes. Absorption spectrum of carotenoids extracted from the Benihayato variety was similar to that of standard ${\beta}-carotene$ indicating that carotenoids in yellow sweet potato are mostly composed of ${\beta}-carotene$.

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Line-shape analysis of the Raman-spectrum from B1g bond buckling phonon in Bi2Sr2CaCu2O8+x

  • Jeong, J.;Oh, D.;Song, D.;Eisaki, H.;Kim, C.;Park, S.R.
    • Progress in Superconductivity and Cryogenics
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    • v.21 no.4
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    • pp.9-12
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    • 2019
  • We performed Raman spectroscopy on two different over-doped Bi2Sr2CaCu2O8+x (BSCCO), of which superconducting transition temperatures are 89 K and 77 K. Line-shape analysis of the Raman-spectrum was done, focused on B1g bond buckling mode which have drawn a lot of attention, since photoemission studies showed an evidence for strong coupling between the mode and electron. The line-shapes show asymmetry and are well fitted by the Fano line-shape formula. Remarkably, we found that the peak line-widths from B1g bond buckling mode in BSCCO show much broader than those in YBa2Cu3O7-x. The broad line width can be attributed to the superstructure modulation of BSCCO. Our results imply that B1g bond buckling mode may have close relation to the origin of superconductivity or to boosting the superconducting transition temperature in BSCCO.