• BMB Reports
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• 제30권2호
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• pp.106-112
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• 1997

The effect of cryopreservation on extracellular matrix was studied with the ultimate objective of permiting a prediction of the tendency of aorta conduit tissue to calcify following transplantation. Cryopreserved and fresh porcine aorta conduit tissues were extracted using guanidine-hydrochloride (Gdn-HCl) followed by sequential digestion of the tissues with collagenase, elastase, and papain. Glycosaminoglycans (GAGs) of the proteoglycans (PGs) were isolated and quantitated. Gdn-HCl extracted about 61% and 62% of the total GAG (proteoqlycan) material from cryopreserved and fresh tissues, respectively. Collagenasesolubilized proteoglycans from Gdn-HCl extracted tissue represented 20% and 13%, respectively, of the total GAGs present in cryopreserved and fresh tissues. Subsequent elastase hydrolysis of collagenase-digested tissue released about 11% of total GAGs from cryopreserved tissue and 16% from fresh tissue. The remaining 8%, from cryopreserved tissue, and 9%, from fresh tissue, of the total GAGs were obtained after using a papain hydrolysis. There was essentially no difference between fresh and cryopreserved tissues in the relative distribution of proteoglycans in the extracts and digestions except in the initial digestion step where more proteoglycans were obtained from collagenase solubilization of cryopreserved tissue than fresh tissue (p<0.05). The histologic status of the fresh and cryopreserved porcine aortic conduit did not differ markedly. The normal tissue architecture was not affected markedly by the cryopreservation procedure as neither alteration of elastic structure, fibrous proteins nor alteration of nuclear distribution or smooth muscle cell morphology was detected. Quantitative tissue mineral studies revealed that the mean calcium content of the cryopreserved aorta conduit tissue $(165{\pm}3\;{\mu}g/g\;wet\;tissue)$ was higher than that of the fresh tissue $(105{\pm}4\;{\mu}g/g\;wet\;tissue)$ $(p<0.05)$. The mean phosphorus content was $703{\pm}35\;{\mu}g$ wet tissue from cryopreserved tissue and $720{\pm}26\;{\mu}g$ wet tissue from fresh tissue. The study indicates that there is no significant alteration in the distribution of PGs in properly cryopreserved tissue, but the total calcium level appears to be increased in tissue cryopreserved by the cryopreservation process used in this study.

• Applied Biological Chemistry
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• 제47권4호
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• pp.403-408
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• 2004

한국산 재래종 호박 완숙과의 총세포벽 물질, 펙틴, 총단백질, 단백질을 구성하고 있는 아미노산 및 유리아미노산의 함량을 비교 조사하였다. 과육의 단백질 함량은 ‘제주 2’가 $2,830\;{\mu}g/g$으로 가장 높았고 '상주'는 $1,319\;{\mu}g/g$으로 가장 낮았다. 세포벽물질은 ‘강화’가 2,961 mg%로 가장 높았고, '남해'는 1,582 mg%로 가장 낮았다. 또한 세포벽 물질 내 펙틴의 함량은 ‘강화'(2,198 mg%), '제주 2'(2,178 mg%) 및 ’제주 1'(1,461 mg%)의 순서로 높았다. 또한 세포벽 물질 내 펙틴은 수용성과 불용성 펙틴이 주류를 이루고 있음을 알 수 있었다. 아미노산 함량조성은 품종에 따라 다소 차이는 있지만 glutamic acid, aspartic acid, lysine, leucine 및 valine이 주를 이루어 전체 아미노산 함량의 50% 이상을 차지하였고, 특이하게도 '제주 2'는 aspartic acid와 threonine이 검출되지 않았다. 한편 완숙과 호박의 과육 내 유리 아미노산은 8종의 필수아미노산(histidine, isoleucine, leucine, lysine, phenylalanine, methionine, threonine 및 valine)을 비롯해 총 34종류의 유리아미노산이 검출되었고 aspartic acid와 asparagine의 함량이 전체 유리아미노산 중 50% 이상으로 가장 높았다. 또한 이뇨작용과 관련된 ornithine, citrulline, arginine이 모든 품종에서 검출되었으며 ornithine과 citrulline은 품종간에 큰 차이가 없었으나 arginine은 ‘남해’가 4.3 mg%이었으며, ‘태안’이 29.34 mg%로 품종간에 7배의 큰 차이를 보였다.

• 생물환경조절학회지
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• 제14권3호
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• pp.196-202
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• 2005

본 연구는 상토의 보수성 증가를 위하여 혼합되는 acrylamide 계통 고흡수성 수지인 Stocksorb C(STSB)의 수분 흡수 특성, 수분 흡수에 영향을 미치는 각종 무기염의 종류 및 농도, STSB가 침지된 외부용액속의 무기염 농도 변화를 구명하고자 수행하였다. STSB의 수분 흡수량은 증류수에 침지한 경우 약 180 $mL{\cdot}g^{-1}$였다. 1가의 양이온을 포함한 염인 $KH_2PO_4,\;KNO_3$ 또는 $(NH_4)_2SO_4$를 용해시킨 용액의 농도가 높아질수록 흡수량이 저하되었으나 세 종류 양이온의 종류에 따른 차이는 크지 않았다. $Ca(NO_3)_2{\cdot}4H_2O$ 다양한 농도로 용해시킨 용액 속에 STSB를 침지시킨 경우 수분흡수능 저하가 심하여 20 $meq{\cdot}L^{-1}Ca(NO_3)_2{\cdot}4H_2O$ 용액에서 약 25 $mL{\cdot}g^{-1}$ 수분을 흡수하였다. $Mg(NO_3)_2{\cdot}6H_2O$나 $MgSO_4{\cdot}7H_2O$를 용해시킨 용액의 농도가 높아질수록 수분 흡수량이 심하게 저하하였고, 1가의 양이온이 포함된 염을 용해시킨 용액에서보다 그 정도가 심하였다. 그러나 요소의 농도를 증가시킨 용액에서의 수분 흡수량은 저하되지 않았다. 침지전 Hoagland용액의 농도가 높거나, 침지시간이 길어질수록 STSB를 침지시킨 용액에서의 $K^+$및 $NH_4^+-N$의 농도가 점차 높아졌다. Hoagland 용액의 희석배수와 무관하게 침지시간이 길어질수록 외부용액의 $NO_3^--N,\;H_2PO_4^-$ 및 $SO_4^{-2}$ 농도가 약간 상승하였고, 24시간 경과 후 각각 5,300, 250 및 1,500 $mg{\cdot}L^{-1}$ 농도로 분석되었다.

• 한국콘텐츠학회논문지
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• 제12권9호
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• pp.244-249
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• 2012

본 연구에서 국내산 겨우살이 lectin이 흰쥐 간의 지방형성에 미치는 영향을 알아보기 위해 혈청 내 지질 함량을 측정하였고, 이를 확인하고자 간조직의 지방형성 변화를 조사하였다. 렉틴의 분리는 sephadex G-150로 여과 및 sepharose 4B affinity chromatography 로 정제하였다. $CCl_4$를 복강 내에 투여시 72시간 경과 후 혈청 총cholesterol 및 triglyceride는 정상군 및 olive oil 투여군에 비해 유의하게 증가하였으며, lectin과 $CCl_4$를 동시 투여 하였을 때는 유의한 감소를 나타내였다. 흰쥐 간의 조직학적 관찰은 $CCl_4$투여군은 정상군 및 olive oil 투여군에 비해 지방변화 및 간세포 내에 많은 대소의 cytoplasmic vacuole가 보였으나, $CCl_4$와 lectin을 동시 투여시는 $CCl_4$ 투여군에 비해 cytoplasmic vacuole은 현저히 감소하였고, 이에 비례하여 혈청 cholesterol 및 triglyceride 측정치가 현저히 감소하는 결과를 나타내었다. 이상의 결과를 볼때 흰쥐에서 lectin이 혈청 지질을 감소시키거나 또는 조직학적 관찰에서 간조직의 지질형성을 억제시키는 효과가 있는 것으로 확인되었다.

• 한국조경학회지
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• 제29권4호
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• pp.91-99
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• 2001

This study was carried out to investigate the effects of mowing height, rolling, N-fertilizing, and season on green speed(i.e., ball-roll distance) for developing and implementing a program of increasing green speed in Korean golf courses. Data were subjected to multi-regression analysis using SPSSWIN(Statistical Package for the Social Science), which collected from Yong-Pyong golf course greens selected to investigate. The results was as follows. 1) The multi-regression analysis of mowing height, rolling times, and N-fertilizer application rates on spring green speed was as follows; $Y_1$(spring green speed)=4.287+0.155X$_1$(rolling times)-0.131X$_2$(the amount of N-fertilizing)-0.251X$_3$(mowing height). 2) The multi-regression analysis of mowing height, rolling times, and N-fertilizer application rates on summer green speed was as follows; $Y_2$(summer green speed)=4.833-0.423X$_3$(mowing height)+0.146X$_1$(rolling times)-0.107X$_2$(the amount of N-fertilizing). 3) The multi-regression analysis of mowing height, rolling times, and N-fertilizer application rates on fall green speed was as follows; $Y_3$(fall green speed)=4.651-0.383X$_3$(mowing height)+0.142X$_1$(rolling times)-0.103X$_2$(the amount of N-fertilizing). 4) As mowing height was lowered by 1mm, green speed increased by 0.251~0.423m. As rolling times increased by 1(one), green speed increased by0.142~0.15m. As the amount of N-fertilizing increased by 1g/$m^2$, green speed decreased by 0.103~0.131m. The season also affected green speed. In comparison with spring green speed, summer green speed decreased by 0.145m and fall green speed decreased by 0.144m.

• 한국조경학회지
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• 제32권6호
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• pp.95-102
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• 2005

This study was carried out to find out the heavy metal contents and the physico-chemical properties for the improvement of dredged soils which widely exist in lowlands of Korea. At first all the average heavy metal contents were close to background level and were much lower than concern level of the Soil Environment Conservation Act of Korea. And the results of physical analyses of soils showed on the average $2.46\~2.74 Mg/m^3$ in Particle density, $0.45\~2.45 kg/kg$ in soil water contents, $0.34\~0.90 Mg/m^3$ in bulk density, $0.67\~0.87m^3/m^3$ in porosity, $2.18\times10^{-5}\~1.20\times10^{-8} m/s$ in saturated hydraulic conductivity, R0.12\~0.65 m^3/m^3$ in available water contents. Finally the results of chemical analyses of soils showed on the average $6.5\~8.2\;in\; pH,\;5\~48 g/kg\;in\; OM,\;0.48\~4.51g/kg\;in\;T-N,\;19\~25mg/kg$ in available phosphate, $0.28\~11.80 dS/m\;in\;EC,\;8.7\~38.1cmol/kg$ in CEC, respectively Accordingly, the physicochemical properties of soils ought to be analyzed accurately before dredging for effective using of dredged soils. And it will be more effective, if the dredged soils are used with proper balance among each content of components with consideration to the physicochemical properties of common soils.

• 한국농촌건축학회논문집
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• 제15권3호
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• pp.17-24
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• 2013

This study examined and investigated architectural characteristics such as the use of buildings, the number of layers, and structure, and roof landscape characteristics such as roof types, materials, and color in rural villages which were located on the street on the assumption that the landscape of rural villages should be managed by considering visual aspects. The results were as follows. The commonest frequency was found in 'residence'(2/3) for the use of buildings and 'masonry structure'(2/3) for the structure. 'Wood structure' and 'light gauge steel structure(prefabricated structure)' were also commonly found. The results suggest that although many rural houses have partially improved by agricultural and fishery development projects and garden suburbs has been increased by city residents' desire for rural life, there have been still many old houses. Frequently used roof types were a gambrel roof and a hipped roof. Roof materials were mixed in several materials such as tiles, slate, panels, color steel plate, reinforced concrete, and asphalt shingles. Roof color was also mixed in several colors such as reddish N7 and N0.5 of Neutral color, 10R3/6 and 10R3/10 of R color, 7.5B4/10 and 7.5B7/8 of B color and 5G8/6 of G color. The result suggests that roof color impedes the landscape of rural villages on the street. Based on the results, some roofs of rural villages were improved by the support of the government or the local governments but there are still many old roofs. The mixture of improved roofs and old roofs mainly contributed to impeding the landscape of rural villages and it was probably caused by the lack of systematic landscape plans by individual improvement of buildings. Therefore, it is necessary to devise systematic landscape plans in consideration of local identity and neighboring environment. In particular, the guideline for roof color influencing a street landscape should be established.

• 생물환경조절학회지
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• 제20권4호
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• pp.341-345
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• 2011

이 연구는 화단용 국화의 식재 효율성을 높이기 위한 목적으로 기존에 개발된 식생매트에 삽수를 이용하여 최적의 매트조건을 구명하고자 수행하였다. 8가지 처리 조건의 매트에 Dendranthema grandiflorum 'Ford'를 삽목하고 난 후 50일 후 생육을 조사한 결과, 매트 내 뿌리 분포는 10mm 두께의 중간에 배양토를 삽입한 coir net(C 처리)에서 가장 좋았고, 초장과 지상부 생체중도 각각 29cm, 5.6g으로 동일 조건에서 가장 높은 값을 나타냈다. 뿌리가 형성된 매트를 포장에 정식 40일 후 초장은 12mm 두께의 중간에 배양토를 삽입한 Jute net가 가장 좋았으나, 지상부 생체중은 C 처리와 차이가 없었다. 매트 내 뿌리 형성과 정식 후 삽수 길이별 생육은 길이 5cm인 삽수가 3과 8cm인 것 보다 좋은 결과를 나타냈다.

• International Journal of Computer Science & Network Security
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• 제23권3호
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• pp.110-122
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• 2023

Over the last few decades, various innovative technologies have emerged that have significantly contributed to making life easier for humans. Various information and communication technologies (ITCs) have emerged as a result of the global technological revolution, including big data, IoT, 4G and 5G networks, cloud computing, mobile computing, and artificial intelligence. These technologies have been adopted in urban planning and development, which gave rise to the concept of smart cities in the 1990s. A smart city is a type of city that uses ITCs to exchange and share information to enhance the quality of services for its citizens. With the global population increasing at unprecedented levels, cities are overwhelmed with a myriad of challenges, such as the energy crisis, environmental pollution, sanitation and sewage challenges, and water quality issues, and therefore, have become a convergence point of economic, social, and environmental risks. The concept of a smart city is a multidisciplinary, unified approach that has been adopted by governments and municipalities worldwide to overcome these challenges. Though challenging, this transformation is essential for cities with differing technological and social features, which all have the potential to determine the success or failure of the digital transformation of cities into smart cities. In recent years, researchers, businesses, and the government have all turned their attention to the emerging field of smart cities. Accordingly, this paper aims to represent a thorough understanding of the movement toward smart cities. The key themes identified are smart city definitions and concepts, smart city dimensions, and smart city architecture of different layers. Furthermore, this article discusses the challenges and some examples of smart cities.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제13권4호
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• pp.231-239
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• 2007

본 논문에서는 국내 표준 128비트 블록 암호화 알고리즘인 SEED를 하드웨어로 설계할 경우 면적-성능간의 trade-off 관계를 보여준다. 본 논문에서 다음 4가지 유형의 설계 구조를 비교한다. (1) Design 1 : 16 라운드 완전 파이프라인 방식, (2) Design 2 : 단일 라운드의 반복 사용 방식 (3) Design 3 : G 함수 공유 및 반복 사용 방식 (4) Design 4 : 단일 라운드 내부 파이프라인 방식. (1),(2),(3)의 방식은 기존의 논문들에서 제안한 각기 다른 설계 방식이며 (4)번 설계 방식이 본 논문에서 새롭게 제안한 설계 방식이다. 본 논문에서 새롭게 제안한 방식은, F 함수 내의 G 함수들을 파이프라인 방식으로 연결하여 면적 요구량을 (2)번에 비해서 늘이지 않으면서도 파이프라인과 공유블록 사용의 효과로 성능을 Design 2와 Design 3보다 높인 설계 방식이다. 본 논문에서 4가지 각기 다른 방식을 각각 실제 하드웨어로 설계하고 FPGA로 구현하여 성능 및 면적 요구량을 비교 분석한다. 실험 분석 결과, 본 논문에서 새로 제안한 F 함수 내부 3단 파이프라인 방식이 Design 1 방식을 제외하고 가장 throughput 이 높다. 제안된 Design 4 가 단위 면적당 출력성능(throughput)면에서 다른 모든 설계 방식에 비해서 최대 2.8배 우수하다. 따라서, 새로이 제안된 SEED 설계가 기존의 설계 방식들에 비해서 면적대비 성능이 가장 효율적이라고 할 수 있다.