• BMB Reports
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• v.30 no.2
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• pp.106-112
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• 1997

The effect of cryopreservation on extracellular matrix was studied with the ultimate objective of permiting a prediction of the tendency of aorta conduit tissue to calcify following transplantation. Cryopreserved and fresh porcine aorta conduit tissues were extracted using guanidine-hydrochloride (Gdn-HCl) followed by sequential digestion of the tissues with collagenase, elastase, and papain. Glycosaminoglycans (GAGs) of the proteoglycans (PGs) were isolated and quantitated. Gdn-HCl extracted about 61% and 62% of the total GAG (proteoqlycan) material from cryopreserved and fresh tissues, respectively. Collagenasesolubilized proteoglycans from Gdn-HCl extracted tissue represented 20% and 13%, respectively, of the total GAGs present in cryopreserved and fresh tissues. Subsequent elastase hydrolysis of collagenase-digested tissue released about 11% of total GAGs from cryopreserved tissue and 16% from fresh tissue. The remaining 8%, from cryopreserved tissue, and 9%, from fresh tissue, of the total GAGs were obtained after using a papain hydrolysis. There was essentially no difference between fresh and cryopreserved tissues in the relative distribution of proteoglycans in the extracts and digestions except in the initial digestion step where more proteoglycans were obtained from collagenase solubilization of cryopreserved tissue than fresh tissue (p<0.05). The histologic status of the fresh and cryopreserved porcine aortic conduit did not differ markedly. The normal tissue architecture was not affected markedly by the cryopreservation procedure as neither alteration of elastic structure, fibrous proteins nor alteration of nuclear distribution or smooth muscle cell morphology was detected. Quantitative tissue mineral studies revealed that the mean calcium content of the cryopreserved aorta conduit tissue $(165{\pm}3\;{\mu}g/g\;wet\;tissue)$ was higher than that of the fresh tissue $(105{\pm}4\;{\mu}g/g\;wet\;tissue)$ $(p<0.05)$. The mean phosphorus content was $703{\pm}35\;{\mu}g$ wet tissue from cryopreserved tissue and $720{\pm}26\;{\mu}g$ wet tissue from fresh tissue. The study indicates that there is no significant alteration in the distribution of PGs in properly cryopreserved tissue, but the total calcium level appears to be increased in tissue cryopreserved by the cryopreservation process used in this study.

• Applied Biological Chemistry
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• v.47 no.4
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• pp.403-408
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• 2004

We studied active substances like crude cell wall components, crude protein, composing amino acids and free amino acids including orinithine cycle-related amino acids such as asparagine, ornithine and citrullin in fully ripe fruits of Korean native squash, Cucurbita moschata Poir. Crude protein content of 'Jeju 2' was the highest with $2,830\;{\mu}g/g$, while 'Sangju' was the lowest with $1,319\;{\mu}g/g$. Regarding the contents of crude cell wall components, fruit 'Kanghaw' was the highest with 2,961 mg% while 'Namhea' was the lowest with 1,582 mg%. Pectin contents of crude cell wall components were the highest in 'Kanghaw' (2,198 mg%) followed by 'Jeju 2' (2,178 mg%) and 'Jeju l' (1,461 mg%). The main contents of amino acids in squash were glutamic acid, aspartic acid, lysine, leucine and valine, which comprised to be more than 50% of total amino acid contents. Especially, in 'Jeju 2' aspartic acid and threonine were not detected. In fully ripe fruits, a total of 34 kinds of free amino acids were detected including 8 kinds of essential amino acids (histidine, isoleucine, leucine, lysine, phenylalanine, methionine, threonine and valine). More than 50% of the total free amino acids were aspartic acid and asparagine, and also all varieties were detected in ornithine, citrullin, and arginine, which are related to Ornithine cycle. There was a big difference in the contents of arginine in all varieties whereas the contents of ornithine and citrullin were very similar. 'Teaan' 29.34% was 7 times higher than 'Namhea' 4.30% in regards to arginine contents.

• Journal of Bio-Environment Control
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• v.14 no.3
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• pp.196-202
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• 2005

This research was conducted to determine the amount of water absorbed by a polyacrylamide hydrogel such as Stocksorb C (STSB), effect of salts on inhibition in hydration of STSB, and the hydrogel effects on changes of nutrient concentration in external solution. Absorption of deionized water by STSB reached a maximum of 180 $mL{\cdot}g^{-1}$. Monovalent soluble salts such as $KH_2PO_4,\;KNO_3$, and $(NH_4)_2SO_4$ reduced absorption of the hydrogel, but the degrees of inhibition in absorption were similar in three kinds of salts. Twenty milliequivalents per liter of $Ca_{2+}\;or\;Mg_{2+}$ reduced water absorption of STSB to $14\%$ compared to those of deionized water. Solution absorption was consistently lower in the presence of divalent cations than in the presence of the monovalent cations. But the absorption was unaffected by the uncharged salt such as urea in all concentrations tested. The final $K^+\;and\;NH_4^+-N$ concentrations of the solution remaining after absorption by STSB was higher than those of the initial solution. The soaking of STSB to full strength of Hoagland solution resulted in increase of $NO_3^--N,\;H_2PO_4^-\;and\;SO_4^{2-}$ concentrations in external solution compared to initial solution, reaching 5,300, 250 and 1,500 $mL{\cdot}g^{-1}$, respectively, at 24 hrs after soaking.

• The Journal of the Korea Contents Association
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• v.12 no.9
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• pp.244-249
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• 2012

This study was carried out to investigate effect of serum lipid and liver tissue in lectin from Viscum album on rats. The lectin was purified by sepharose 4B affinity chromatography and gel filtration using sephadex G-150 with plant material from Viscum album. After 72 h of $CCl_4$ injection, there was a significant increase in serum total cholesterol and triglycerige levels relative to the control group. However, treatment of both Viscum album and purified lectin were significantly decreased lipid parameters against the $CCl_4$-induced. Histological observation of the liver section of rats challenged with $CCl_4$ produced a marked increase of cytoplasmic vacuoles in number, while rats administered olive oil alone did not alter the normal hepatic architecture. Histological observation of the liver section in rats treated 72 h with either Viscum album purified lectin or $CCl_4$-induced liver lipogenesis showed decreased numbers of cytoplasmic vaculoes and necrotic cells. These results suggest that Viscum album lectin has a significantly decreased lipid in serum or histological observation of the liver section decreased in lipogenesis in rats.

• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.4
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• pp.91-99
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• 2001

This study was carried out to investigate the effects of mowing height, rolling, N-fertilizing, and season on green speed(i.e., ball-roll distance) for developing and implementing a program of increasing green speed in Korean golf courses. Data were subjected to multi-regression analysis using SPSSWIN(Statistical Package for the Social Science), which collected from Yong-Pyong golf course greens selected to investigate. The results was as follows. 1) The multi-regression analysis of mowing height, rolling times, and N-fertilizer application rates on spring green speed was as follows; $Y_1$(spring green speed)=4.287+0.155X$_1$(rolling times)-0.131X$_2$(the amount of N-fertilizing)-0.251X$_3$(mowing height). 2) The multi-regression analysis of mowing height, rolling times, and N-fertilizer application rates on summer green speed was as follows; $Y_2$(summer green speed)=4.833-0.423X$_3$(mowing height)+0.146X$_1$(rolling times)-0.107X$_2$(the amount of N-fertilizing). 3) The multi-regression analysis of mowing height, rolling times, and N-fertilizer application rates on fall green speed was as follows; $Y_3$(fall green speed)=4.651-0.383X$_3$(mowing height)+0.142X$_1$(rolling times)-0.103X$_2$(the amount of N-fertilizing). 4) As mowing height was lowered by 1mm, green speed increased by 0.251~0.423m. As rolling times increased by 1(one), green speed increased by0.142~0.15m. As the amount of N-fertilizing increased by 1g/$m^2$, green speed decreased by 0.103~0.131m. The season also affected green speed. In comparison with spring green speed, summer green speed decreased by 0.145m and fall green speed decreased by 0.144m.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.6 s.107
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• pp.95-102
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• 2005

This study was carried out to find out the heavy metal contents and the physico-chemical properties for the improvement of dredged soils which widely exist in lowlands of Korea. At first all the average heavy metal contents were close to background level and were much lower than concern level of the Soil Environment Conservation Act of Korea. And the results of physical analyses of soils showed on the average $2.46\~2.74 Mg/m^3$ in Particle density, $0.45\~2.45 kg/kg$ in soil water contents, $0.34\~0.90 Mg/m^3$ in bulk density, $0.67\~0.87m^3/m^3$ in porosity, $2.18\times10^{-5}\~1.20\times10^{-8} m/s$ in saturated hydraulic conductivity, R0.12\~0.65 m^3/m^3$ in available water contents. Finally the results of chemical analyses of soils showed on the average $6.5\~8.2\;in\; pH,\;5\~48 g/kg\;in\; OM,\;0.48\~4.51g/kg\;in\;T-N,\;19\~25mg/kg$ in available phosphate, $0.28\~11.80 dS/m\;in\;EC,\;8.7\~38.1cmol/kg$ in CEC, respectively Accordingly, the physicochemical properties of soils ought to be analyzed accurately before dredging for effective using of dredged soils. And it will be more effective, if the dredged soils are used with proper balance among each content of components with consideration to the physicochemical properties of common soils.

• Journal of the Korean Institute of Rural Architecture
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• v.15 no.3
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• pp.17-24
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• 2013

This study examined and investigated architectural characteristics such as the use of buildings, the number of layers, and structure, and roof landscape characteristics such as roof types, materials, and color in rural villages which were located on the street on the assumption that the landscape of rural villages should be managed by considering visual aspects. The results were as follows. The commonest frequency was found in 'residence'(2/3) for the use of buildings and 'masonry structure'(2/3) for the structure. 'Wood structure' and 'light gauge steel structure(prefabricated structure)' were also commonly found. The results suggest that although many rural houses have partially improved by agricultural and fishery development projects and garden suburbs has been increased by city residents' desire for rural life, there have been still many old houses. Frequently used roof types were a gambrel roof and a hipped roof. Roof materials were mixed in several materials such as tiles, slate, panels, color steel plate, reinforced concrete, and asphalt shingles. Roof color was also mixed in several colors such as reddish N7 and N0.5 of Neutral color, 10R3/6 and 10R3/10 of R color, 7.5B4/10 and 7.5B7/8 of B color and 5G8/6 of G color. The result suggests that roof color impedes the landscape of rural villages on the street. Based on the results, some roofs of rural villages were improved by the support of the government or the local governments but there are still many old roofs. The mixture of improved roofs and old roofs mainly contributed to impeding the landscape of rural villages and it was probably caused by the lack of systematic landscape plans by individual improvement of buildings. Therefore, it is necessary to devise systematic landscape plans in consideration of local identity and neighboring environment. In particular, the guideline for roof color influencing a street landscape should be established.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.341-345
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• 2011

This study was aimed to select the optimal mat condition using existing plant-mats for the efficient planting of bedding Chrysanthemum. At fifty days after cutting with Dendranthema grandiflorum 'Ford', root formation among the treatments using eight different mats outstood with the treatment using 10 mm thick coir net, which has medium inserted between mat layers - called C treatment, compared to other treatments; this treatment had the highest values in the plant height and shoot fresh weight, which were 29 cm and 5.6 g, respectively. On the contrary, in 40 days after transplanting root-formed mats to field, 12 mm thick jute net, which has medium inserted between mat layers, had the highest plants compared to other treatments. However, there was no significant difference in shoot weight compared to C treatment. In experiment of different lengths of cut, the results of growth after transplanting showed that 5 cm long cut performed best compared to 3 and 8 cm long cuts.

• International Journal of Computer Science & Network Security
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• v.23 no.3
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• pp.110-122
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• 2023

Over the last few decades, various innovative technologies have emerged that have significantly contributed to making life easier for humans. Various information and communication technologies (ITCs) have emerged as a result of the global technological revolution, including big data, IoT, 4G and 5G networks, cloud computing, mobile computing, and artificial intelligence. These technologies have been adopted in urban planning and development, which gave rise to the concept of smart cities in the 1990s. A smart city is a type of city that uses ITCs to exchange and share information to enhance the quality of services for its citizens. With the global population increasing at unprecedented levels, cities are overwhelmed with a myriad of challenges, such as the energy crisis, environmental pollution, sanitation and sewage challenges, and water quality issues, and therefore, have become a convergence point of economic, social, and environmental risks. The concept of a smart city is a multidisciplinary, unified approach that has been adopted by governments and municipalities worldwide to overcome these challenges. Though challenging, this transformation is essential for cities with differing technological and social features, which all have the potential to determine the success or failure of the digital transformation of cities into smart cities. In recent years, researchers, businesses, and the government have all turned their attention to the emerging field of smart cities. Accordingly, this paper aims to represent a thorough understanding of the movement toward smart cities. The key themes identified are smart city definitions and concepts, smart city dimensions, and smart city architecture of different layers. Furthermore, this article discusses the challenges and some examples of smart cities.

• Journal of KIISE:Computing Practices and Letters
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• v.13 no.4
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• pp.231-239
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• 2007

This paper presents the trade-off relationship between area and performance in the hardware design space exploration for the Korean national standard 128-bit block cipher algorithm SEED. In this paper, we compare the following four hardware design types of SEED algorithm : (1) Design 1 that is 16 round fully pipelining approach, (2) Design 2 that is a one round looping approach, (3) Design 3 that is a G function sharing and looping approach, and (4) Design 4 that is one round with internal 3 stage pipelining approach. The Design 1, Design 2, and Design 3 are the existing design approaches while the Design 4 is the newly proposed design in this paper. Our new design employs the pipeline between three G-functions and adders consisting of a F function, which results in the less area requirement than Design 2 and achieves the higher performance than Design 2 and Design 3 due to pipelining and module sharing techniques. We design and implement all the comparing four approaches with real hardware targeting FPGA for the purpose of exact performance and area analysis. The experimental results show that Design 4 has the highest performance except Design 1 which pursues very aggressive parallelism at the expanse of area. Our proposed design (Design 4) shows the best throughput/area ratio among all the alternatives by 2.8 times. Therefore, our new design for SEED is the most efficient design comparing with the existing designs.