• 무역상무연구
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• 제78권
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• pp.53-72
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• 2018

Recently, it has been known that it need to be solved export marketing on expanding export of Small Business Commodity. Therefore, the purpose of this paper is to analyse on expanding export of Small Business Commodity through e-Trading Application in Industrie 4.0. This study deals with the terms of three connection success factors on expanding export of Small Business Commodity through e-Trading Application in Industrie 4.0 which are a firm's subjective factors, a industrial environment factors, and a governmental policy factors. According to analysis results of the three success factors, a firm's subjective factors(4.13 score) are scored at the most ones of the three success factors, to be compared with a industrial environment factors(3.89 score), with a government policy factors(3.72 score). Therefore, first of all, it is important to expanding export of Small Business Commodity through e-Trading Application in Industrie 4.0 through as follows, a firm's subjective factors : (1) to procure concentrated market strategy and real market capacity, (2) to procure speedy satisfaction of customer needs and confidence, (3) to procure ability of export marketing through e-Trading Application, (4) to enhance export expanding strategy coincided in Industrie 4.0. And, the next, we have to expanding export of Small Business Commodity through e-Trading Application in Industrie 4.0 through considering a industrial environment factors and a government policy factors.

• 약학회지
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• 제46권5호
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• pp.313-319
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• 2002

Synthesis of 7$\beta$-[(Z)-2-(2-aminothiazol-4-yl)-2-(1-carboxy-1-methylethoxyimino)acetamido]-3-[[(3S, 5S)-5-[4-phenyl-5-(4-methylphenyl or 2-thiophenyl)-4H-l, 2, 4- triazol-3-yl]thiomethylpyrrolidin-3-yl]]thiomethyl-3-cephem-4-carboxylic acids (7a, 7b) were described. (2S, 4S)-4-acethylthio-2-[4-phenyl-5-(4-methylphenyl or 2-thiophenyl)-4 H-1, 2, 4-triazol-3-yl]thiomethyl-1-tert-butoxycarbonylpyrrolidines (4a, 4b) were prepared from trans-4-hydroxy-L-proline with (2S, 4R)-absolute configuration as starting material. 4-Phenyl-5-(4-methylphenyl or 2-thiophenyl)-4 H-l, 2, 4-triazol-3-thiols (2a, 2b) were prepared from p-toluic anhydride and 2-thiophene carboxylic acid hydrazide, respectively. p-Methoxybenzyl 7$\beta$-(Z)-2-(2-for-mamidothiazol-4-yl)-2-(1-tert-butoxycarbonylisopropylimino]acetamido-3-[[ (3S, 5S)-5-[4-phenyl-5-(4-methylphenyl or 2-thio phenyl)-4H-1, 2, 3-triazol-3-yl]thiomethyl-1- tert-butoxycarbonylpyrrolidin-3-yl]]thiomethyl-3-cephem-4-carboxylates (6a, 6b) were achieved by using p-methoxybenzyl ]7P-(Z)-2-(2-formamidothiazol-4-yl)-2-(tert-butoxycarbonylisopropylimino] acetamido-3-chloromethyl-3-cephem-4-carboxylate (5) and (2S, 4S)-4-acethylthio-2-[4-phenyl-5-(4-methyl phenyl or 2-thiophenyl)-4H-1, 2, 4-triazol-3-yl]thiomethyl-1-tert-butoxycarbonyl pyrrolidines (4a, 4b). Removal of formyl, Boc, and p-methoxybenzyl protecting groups were carried out by triflu oroacetic acid and anisole to give the target compounds.

• Archives of Pharmacal Research
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• 제13권3호
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• pp.257-260
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• 1990

Hantzch's type reaction of methyl acetopyruvate (2a), methyl 3-aminocrotonate (3) and 3-nitrobenzaldehyde (4) led to dimethyl 3-acetyl-6-methyl-4-(3'-nitrophenyl)-2, 5-dicarboxylate (5a) and methyl 2, 6-dimethyl-5-(1', 2'-dioxo-2'-methoxyethyl)-4-(3' nitrophenyl)- 2, 5-dicarboxylate (5a) and methyl 2, 6-dimethyl-5-(1', 2'-dioxo-2'methoxyethyl_4-(3' nitrophenyl)1, 4-dihydropyridine-3-carboxylate (6a) in 26.7 and 9.2% yield, respectively. On the other hand, methyl 2, 60dimethyl-4-(3'-nitrophenyl)-1, 4-dihydropyridine 3-carboxylate (9) was acylated by ethyl oxaly chloride to give methyl 2, 6-dimethyl-5-(1', 2'-dioxo-2'-ethoxyethyl)-4-(3'-nitrophenyl)-a, 4-dihydropyridine-3-carboxylate (6b) in 76.8% yield.

• Archives of Pharmacal Research
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• 제16권2호
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• pp.164-167
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• 1993

Arylhydrazones of diethyl acetonedicarboxylate 3 was treated with fomaldehyde to 1-aryl-1, 4, 5, 6-tetrahyeheypyridazine derivatives 4a-f Cyclization of compound 4a-f by hydroxy-lamine afforded [3, 4-d] 1, 4, 5, 6-tetrahydropyridazine derivative 5a-f. Also, cyclization of compound 4c with semicarbazide gave pyrazolote [4, 3-c] pyridazine 6. On the other hand compound 3 reacted with ethylothofomate to give diethyl-1, 4-dihydro-1-arypyridazine-4-one-3, 5 dicaboxylate 7, which on treatment with hydrazine, semicarbazide and thiosemicarbazide gave pyridazine, amido and thioamido derivatives. The spectrl and antimicrobial data of these compounds 1-8 were studied.

• 한국식품과학회지
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• 제52권6호
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• pp.595-603
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• 2020

토란에 존재하는 점질다당의 새로운 이용방안을 모색하기 위하여 토란으로부터 다당을 분리하여 항보체 활성을 평가하고 구조 분석을 행하였다. 토란으로부터 분리한 조다당 CE를 이온교환수지와 Sephadex G-100 column를 이용하여 정제하였고, 그 중 수율과 활성이 양호한 CE-4a를 최종 획분으로 선정하였다. 초기 면역반응에 중요한 역할을 하는 보체계에 대한 토란 다당의 활성화 여부를 측정한 결과 양성대조군인 PSK에 준하는 강력한 항보체 활성을 보였고, 시료의 농도차이를 두어 실험한 결과 농도 의존적임을 알 수 있었다. CE-4a는 분자량 약 182.4 kDa의 다당체로 구성당 조성을 확인한 결과 Man, Gal 및 GalA를 높은 비율로 함유하고 있었다. 본 당쇄의 결합양식을 규명하기 위하여 methlyation analysis를 행한 결과 CE-4a는 terminal Galp. 3-linked-Galp, 4-linked Manp, 2,4,6-linked Manp를 포함한 총 10종의 결합으로 구성되어 있었다. 또한 CE-4a의 전체구조를 추정하기 위하여 endo-α-(1→4)-polygalacturonase, exo-α-galactosidase 및 endo-β-(1→4)-mannanase를 이용한 연속 가수분해 처리 및 해석을 행하였다. 결과를 종합하면, 토란 유래 다당 CE-4a는 (1→4)-mannan 주쇄로 존재하며 주쇄인 mannose의 C(O)6 위치에서 한가닥 또는 C(O)2, C(O)6 위치에서 동시에 두가닥의 측쇄가 연결되어 존재하고, 측쇄는 주로 galacto oligo당이 측쇄로 분지된 특징이 있음을 확인할 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제18권4호
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• pp.409-415
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• 1997

Several calixarene derivatives of 5,11,17,23-tetra-tert-butyl-25-(3,5-dinitrobenzoyloxy)-26,27,28-trihydroxycalix[4]arene 2 were synthesized by the reaction of 2 with several acyl and alkylating agents in the presence of base such as pyridine and K2CO3 in THF. Acylation of monobenzoylated p-tert-butylcalix[4]arene 2 yielded their corresponding 1,3-diacylated calix[4]arenes 3a-3g. On the other hand, alkylation of 2 produced a variety calix[4]arene derivatives such as 1,2- and 1,3-disubstituted calix[4]arenes 4a-4c, 4e-4f, or 1,2,4-trisubstituted calix[4]arene 4d. 1,2-Disubstituted calix[4]arenes are chiral. All derivatives exist as a cone conformation based on NMR studies.

• 생명과학회지
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• 제15권1호
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• pp.80-86
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• 2005

생후 7주령 흰쥐를 대상으로 저강도집단, 중강도집단, 고강도집단, 통제집단으로 분리하여 8주 동안 트레드밀 운동을 실시한 후 심장을 적출하여 조직의 세포기질을 분리하고 전기영동을 실시하여 세포기질 LDH 동위효소의 분포양상을 확인하였고, 활성을 측정하였다. 또한 미토콘드리아를 순수분리한 뒤 분리된 미토콘드리아를 외막 내막 막간물질, 매트릭스로 각각 분리하여 각 분획을 전기영동을 실시하고 효소활성을 측정하였다. 운동 강도에 따른 장기간의 지구성훈련으로 심장조직의 세포기질 LDH 동위효소 활성은 운동집단과 비 운동집단에서 모든 LDH 동위효소의 활성을 나타냈다. 세포기질 LDH 동위효소의 운동 강도에 따른 활성변화는 모든 동위효소가 운동강도의 증가에 따라 증가하는 결과를 나타냈으며 모든 강도에서 $AB_3$ 동위효소의 활성이 가장 크게 나타났다. 심장조직 미토콘드리아 각 분획에서 운동 강도에 따른 LDH 등위효소의 분포와 활성은 외막의 경우 다른 분획에 비하여 동위효소의 분포 및 활성이 매우 약하게 나타났다. 운동강도가 증가하면서 모든 분획의 동위효소가 $B_4$쪽으로 활성이 강해지는 현상을 보이며, 외막에서 내막, 매트릭스로 이동하면서 각 분획에 LDH 동위효소의 분포도 많아지고 활성도 또한 증가하는 현상을 나타냈다.

• 한국전기전자재료학회논문지
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• 제32권2호
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• pp.165-173
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• 2019

Lithium anodes (13, 15, 17, and 20 wt% Li) were fabricated by mixing molten lithium and iron powder, which was used as a binder to hold the molten lithium, at about $500^{\circ}C$ (discharge temp.). In this study, the effect of applied pressure and lithium content on the discharge properties of a thermal battery's single cell was investigated. A single cell using a Li anode with a lithium content of less than 15 wt% presented reliable performance without any abrupt voltage drop resulting from molten lithium leakage under an applied pressure of less than $6kgf/cm^2$. Furthermore, it was confirmed that even when the solid electrolyte is thinner, the Li anode of the single cell normally discharges well without a deterioration in performance. The Li anode of the single cell presented a significantly improved open-circuit voltage of 2.06 V, compared to that of a Li-Si anode (1.93 V). The cut-off voltage and specific capacity were 1.83 V and $1,380As\;g^{-1}$ (Li anode), and 1.72 V and $1,364As\;g^{-1}$ (Li-Si anode). Additionally, the Li anode exhibited a stable and flat discharge curve until 1.83 V because of the absence of phase change phenomena of Li metal and a subsequent rapid voltage drop below 1.83 V due to the complete depletion of Li at the end state of discharge. On the other hand, the voltage of the Li-Si anode cell decreased in steps, $1.93V{\rightarrow}1.72V(Li_{13}Si_4{\rightarrow}Li_7Si_3){\rightarrow}1.65V(Li_7Si_3{\rightarrow}Li_{12}Si_7)$, according to the Li-Si phase changes during the discharge reaction. The energy density of the Li anode cell was $807.1Wh\;l^{-1}$, which was about 50% higher than that of the Li-Si cell ($522.2Wh\;l^{-1}$).

• 대한화학회지
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• 제46권4호
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• pp.330-336
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• 2002

새로운 ${\alpha},{\beta}$-불포화Carboxanilide계열의 살균제를 개발을 목적으로 4-acetyl-3-methyl-N-phenyl-1,4-thiazine-2-Carboxamide(6)의 합성에 대하여 기술하였다. Dihydro-1,4-thiazine methyl ester 11을 산화하여 생성된 sulfoxide 7을 Pummerer 반응하여 $\alpha$-acetoxy dihydro-1,4-thiazine 10a를 얻었다. 반며에 Dihydro-1,4-thiazine Carboxanilide sulfoxide14는 동일한 반응조건 하에서 중간체 sulfonium ion의 carboxanilide기가 관여한 vinylogous Pummerer 반응의 전위에 의하여 중간체를 15를 통하여 acetoxymethyl dihydro-1,4-thiazine 18이 생성되었다. 1,4-Thiazine carboxanilide 6은 $\alpha$-acetoxy dihydro-1,4-thiazine 10a를 산촉매로 처리한 다음 생성된 21a를 가수분해하고 아닐린과 결합하여 합성하였다

• Biomolecules & Therapeutics
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• 제21권6호
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• pp.487-492
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• 2013

Cytochrome P450 4A11 (CYP4A11) is a fatty acid hydroxylase enzyme expressed in human liver. It catalyzes not only the hydroxylation of saturated and unsaturated fatty acids, but the conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), a regulator of blood pressure. In this study, we performed a directed evolution analysis of CYP4A11 using the luminogenic assay system. A random mutant library of CYP4A11, in which mutations were made throughout the entire coding region, was screened with luciferase activity to detect the demethylation of luciferin-4A (2-[6-methoxyquinolin-2-yl]-4,5-dihydrothiazole-4-carboxylic acid) of CYP4A11 mutants in Escherichia coli. Consecutive rounds of random mutagenesis and screening yielded three improved CYP4A11 mutants, CP2600 (A24T/T263A), CP2601 (T263A), and CP2616 (A24T/T263A/V430E) with ~3-fold increase in whole cells and >10-fold increase in purified proteins on the luminescence assay. However, the steady state kinetic analysis for lauric acid hydroxylation showed the significant reductions in enzymatic activities in all three mutants. A mutant, CP2600, showed a 51% decrease in catalytic efficiency ($k_{cat}/K_m$) for lauric acid hydroxylation mainly due to an increase in $K_m$. CP2601 and CP2616 showed much greater reductions (>75%) in the catalytic efficiency due to both a decrease in $k_{cat}$ and an increase in Km. These decreased catalytic activities of CP2601 and CP2616 can be partially attributed to the changes in substrate affinities. These results suggest that the enzymatic activities of CYP4A11 mutants selected from directed evolution using a luminogenic P450 substrate may not demonstrate a direct correlation with the hydroxylation activities of lauric acid.