• 한국동물학회지
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• 제38권3호
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• pp.340-347
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• 1995

D. melanogaster를 대상으로 두 종류의 P element construct, 즉 Pc[(ry+)B]와 p[(ry+)$\Delta$SX9]을 사용한 형질전환 실험을 통하여 자율적 및 비자율적인 P element construct간의 vector로써의 효율성을 분석했다. 자율적인 P element 내에 rosy 유전자를 포함하고 있는 Pc[(ry+)B] construct를 진정 M 계통형인 ry506 돌연변이 계통에 주입시켰다. 또한 비자율적인 P element 내에 rosy 유전자를 포함하고 있는 p[(ry+)$\Delta$SX9] construct와 전이효소의 공급원으로써 p-$\Delta$2-3hs$\pi$ helper plasmid를 역시 같은 계통의 ry506 돌연변이 계통에 주입시켰다. Dechorination 방법에 의해 총 1143 embryo를 대상으로 microinjection 한 결과, 모두 35 계통의 형질전환된 정상형의 눈을 가진 계통을 얻었다. P element construct를 주입시킨 전체 1143 embryo 중에서 약 20% 정도가 성체로 부화되었으며, 부화된 개체 가운데 약 40% 정도가 눈 색깔이 거의 정상형과 같은 G0 transformant 로 나타났다. 그러나 생식력을 가진 G0 transformant의 약 40% 만이 G1 transformant로 나타남으로써 모두 35 계통의 transformant를 얻었다. 따라서 G0 단계에서 나타난 형질전환 현상에는 반드시 rosy transformant의 염색체 삽입에 의해서 만이 아니라 세포질내에서도 construct내의 rosy 유전자의 발현이 가능한 것으로 분석되었다. 또한 형질전환율에 있어서는 Pc[(ry+)B]와p[(ry+)$\Delta$SX9] construct 간에 유의한 차가 없는 것으로 나타났다. 이상의 결과로 볼 때, 실험 목적에 따라 자율적인 P element 또는 helper DNA를 이용한 비자율적인 P element를 효과적인 vector 로 선택 이용함으로써 특정 유전자를 곤충집단내로 침투 및 고정시킬 수 있다고 판단된다.

• Journal of Gastric Cancer
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• 제21권4호
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• pp.325-334
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• 2021

Purpose: Although dumping symptoms are thought to involve postprandial glycemic changes, postprandial glycemic variability without dumping symptoms remains poorly understood due to the lack of a method that allows the easy and continuous measurement of blood glucose levels. Materials and Methods: Patients having undergone distal gastrectomy with Billroth-I (DG-BI) or Roux-en-Y reconstruction (DG-RY), total gastrectomy with RY (TG-RY) and pylorus preserving gastrectomy (PPG) for gastric cancer 3 months to 3 years prior, diagnosed as pathological stage I or II, were prospectively enrolled from March 2018 to January 2020. The interstitial tissue glycemic levels were measured every 15 min, up to 14 days by continuous glucose monitoring. Moreover, using a diary recording the diet and symptoms, asymptomatic glucose profiles without sugar supplementation within 3 h postprandially were compared among the four procedures. Results: A total of 40 patients were enrolled, 10 patients for each of the four procedures. There were 47 glucose profiles with DG-BI, 46 profiles with DG-RY, 38 profiles with TG-RY, and 46 profiles with PPG. PPG showed the slowest increase with a subsequent gradual decrease in glucose fluctuations, without hyperglycemia or hypoglycemia, among the four procedures. In contrast, TG-RY and DG-RY showed spike-like glycemic variability, sharp rises during meals, and rapid drops. The glucose profiles of DG-BI were milder than those of RY. Conclusions: The asymptomatic glycemic changes after meals differ among the types of surgical procedures for gastric cancer. Given the mild glycemic fluctuations in PPG and the glucose spikes in TG-RY and DG-RY, pylorus preservation and physiological reconstruction without changes in food pathways may optimize postprandial glucose profiles after gastrectomy.

• 한국환경농학회지
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• 제27권4호
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• pp.343-348
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• 2008

시설재배 토마토(To) 생산에 미치는 녹비 토양투입 효과와 양분 수지량을 조사하기 위하여 겨울과 여름 휴한기 동안 헤어리베치(Hv), 호밀(Ry), 콩(Sb), 수단그라스(Sd)를 녹비로 재배하였으며, 녹비와 토마토를 조합한 작부체계로서 두과 녹비작물을 활용한 Hv-To-Sb-To, 화본과 녹비작물로 구성된 Ry-To-Sb-To, 그리고 두과와 화본과 녹비를 교호 재배하는 Ry-To-Sd-To로 구분하여, 화학비료(Cf)를 처리하는 Cf-To-Cf-To 작부체계와 비교하였다. 녹비 수량은 겨울 휴한기에 비해 여름 휴한기 동안 높았던 반면, 녹비의 질소 고정량은 화본과에 비해 두과 녹비작물에서 현저히 높아 두과 녹비작물의 경우 토마토 재배를 위한 질소량의 62%를 고정하였다. 두과 녹비작물로 구성된 작부체계(Hv-To-Sb-To)의 토마토 수량은 관행(Cf-To-Cf-To)과 유사하였으나, 화본과 녹비로 구성된 처리구(Ry-To-Sd-To)의 수량은 두과 녹비작물 처리구 수량의 40% 수준으로서 현저한 수량 감소가 나타났다. 한편 두과 녹비작물 활용에 따른 질소공급 효과로 인해 토마토 생육 및 과실 생산량은 증가한 반면, 후작물에 의한 양분흡수량이 많아 Ry-To-Sb-To 작부체계의 N:P:K 수지량은 -80:-59:$-480\;kg\;ha^{-1}\;yr^{-1}$로서 양분의 불균형이 심각하였다. 역으로 화본과 녹비로 구성된 Ry-To-Sb-To 작부체계의 N:P:K 수지량은 43:1:$130\;kg\;ha^{-1}\;yr^{-1}$로서 양분수지상 3요소 모두 양의 값을 보였으나, 이는 화본과 녹비작물의 낮은 질소 고정량과 높은 C/N율로 인해 후작물 토마토의 생육이 부진하였던 결과로 판단된다. 결론적으로 두과 녹비작물 토양 환원시 양분 수지량에서 큰 음의 값을 보였을지라도 관행수준의 토마토 수량을 얻을 수 있다는 관점에서 두과녹비는 화학비료 없이 유기농 재배를 위한 양분공급 및 토양지력 증진 방안으로서 활용 가능하고, 일반 토마토 재배 시에도 다소의 부족한 양분 수지량만 조정한다면 균형된 작부체계로 이용할 수 있을 것으로 판단된다.

• International Journal of Oral Biology
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• 제40권4호
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• pp.211-216
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• 2015

Nitric Oxide (NO) is an important signaling molecule in the nociceptive process. Our previous study suggested that high concentrations of sodium nitroprusside (SNP), a NO donor, induce a membrane hyperpolarization and outward current through large conductances calcium-activated potassium ($BK_{ca}$) channels in substantia gelatinosa (SG) neurons. In this study, patch clamp recording in spinal slices was used to investigate the sources of $Ca^{2+}$ that induces $Ca^{2+}$-activated potassium currents. Application of SNP induced a membrane hyperpolarization, which was significantly inhibited by hemoglobin and 2-(4-carboxyphenyl) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt (c-PTIO), NO scavengers. SNP-induced hyperpolarization was decreased in the presence of charybdotoxin, a selective $BK_{Ca}$ channel blocker. In addition, SNP-induced response was significantly blocked by pretreatment of thapsigargin which can remove $Ca^{2+}$ in endoplasmic reticulum, and decreased by pretreatment of dentrolene, a ryanodine receptors (RyR) blocker. These data suggested that NO induces a membrane hyperpolarization through $BK_{ca}$ channels, which are activated by intracellular $Ca^{2+}$ increase via activation of RyR of $Ca^{2+}$ stores.

• 대한약리학회지
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• 제31권2호
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• pp.207-217
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• 1995

To investigate the functional and immunological properties of the Ca-release channel in the sarcoplasmic reticulum(SR) of the eel skeletal muscle, $[^3H]ryanodine$ binding, SDS gel electrophoresis, $^{45}Ca\;release$ studies, and immunoblot assay were carried out in the SR of the eel skeletal muscle. Maximal binding sites(Bmax) and $K_D$ values of $[^3H]ryanodine$ for Ca-release channel of the SR of the eel skeletal muscle were $19.44{\pm}1.40\;pmole/mg$ protein and $15.55{\pm}1.69\;nM$, respectively. $[^3H]Ryanodine$ binding to RyR was increased by calcium and AMP. The SR of the eel skeletal muscle has two high molecular weight bands on the SDS PAGE. The mobility of upper band was more slower than the single band of the rabbit skeletal muscle, and that of the lower band was similar with the single band of canine cardiac muscle. Vesicular $^{45}Ca-release$ was activated by calcium. Ca-induced $^{45}Ca-release$ was significantly inhibited by $MgCl_2(2\;mM)$, ruthenium red$(10\;{/mu}M)$ or tetracaine(1 mM), but not by high concentration of calcium itself. AMP-induced $^{45}Ca-release$ was slightly occurred only in the absence of calcium, it was not inhibited by $MgCl_2$ or ruthenium red. Caffeine also increased $^{45}Ca-release$ from the SR vesicles, but it was not affected by $MgCl_2$ or ruthenium red. Polyclonal Ab against rat skeletal muscle RyR is reacted with that of rabbit, but not reacted with that of the eel skeletal muscle. These results suggested that ryanodine receptor of the SR of the eel skeletal muscle is showing some similar properties with that of mammalian skeletal muscle, but might be an another isotype channel having two bands which is less sensitive to AMP, not cross-reacted with antisera against rat RyR, and not inhibited by high concentration of calcium.

• 정보학연구
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• 제3권4호
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• pp.23-40
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• 2000

본 연구에서는 기존의 신용장결제방식의 한계성을 극복하면서 전자상거래시대에 부합되도록 최근 개발된 TradeCard의 본질, 업무절차 및 효용성을 분석하고 TradeCard의 폭넓은 활용을 제한하는 문제점과 해결과제를 구체적으로 제시함으로써 무역실무자들에게 TradeCard의 기본원리를 제공해줌을 목적으로 한다.

• 한국의류학회지
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• 제38권6호
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• pp.929-941
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• 2014

Dyeability of Indian natural dyestuffs to fabrics was investigated. Indian dyestuffs are more inexpensive than domestic dyestuffs purchased at oriental medicine stores. We studied the dyeability of madder, marigold, and pomegranate imported from India on cotton, silk, and wool fabrics. Dyebaths of a combination ratio of 80:20, 60:40, 50:50, 40:60 and 20:80 of madder and marigold, fabrics with orange colors were dyed. To evaluate the dyeability of dyed fabrics, K/S values, Munsell color values and CIE L, a, b, ${\Delta}E$ were measured. The dyeability of fabrics dyed in dyebaths of pH 4 and pH 7 were higher than pH 10. The dyeability in pH 4 was better than pH 7. Silk fabrics dyed with madder and marigold showed good dyeability and wool fabrics showed good affinity in madder dyebath and fair affinity in marigold dyebath. Cotton fabrics showed fair affinity in a marigold dyebath of pH 4 and pH 7. Cotton fabrics dyed with a 60:40 ratio of madder and marigold showed 4.76YR of Munsell color value. Silk Fabrics dyed with a 40:60 ratio showed 4.76YR and wool fabrics dyed with 20:80 ratio showed 5.57RY. The ratios produced the closest colors to 5.0YR of orange. This result indicated that marigold had a more powerful effect on cotton fabrics while madder was stronger on wool fabrics. Fading grades of washing colorfastness of wool and silk fabrics dyed in mixed dyebaths were higher than 3.5-4.0 and higher than homogeneous dyebaths. Staining grades of washing colorfastness of all dyed fabrics were between grades 3.5-5.0. Colorfastness to dry-cleaning was high as grades 4.0-5.0 in all of dyed fabrics. Colorfastness to light of dyed fabrics showed a fair grade of 3.5-5.0; in addition, wool fabrics showed lower grades than silk and cotton fabrics.

• Journal of Astronomy and Space Sciences
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• 제3권1호
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• pp.1-13
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• 1986

A long-term project(ten-year ; 1982-92) for the photoeletric observation in the UBV passbands of selected eclipsing binaries with P$\geq$10 days has initiated at Yonsei University Observatory using 40-cm and 61-cm reflectors. the instrumentatio used and the observation techniques and the reduction procedures applied to this investiagation are described. Out of 39 program stars, successful results have been obtained during our first two years for the 11 stars listed below, with the orbital period in days in the parenthesis, and the incomplete light curves of these stars are presented. SX Cas (36.57) AQ Cas (11.72) UU Cnc (96.71) RY Gem (9.30) V373 Cas (13.42) NY Cep (15.28) RX Cas (32.32) V396 Cas (15.28) ZZ Cnc (25.60) Zet Aur (982.2) Eps Aur (9885.) For the rest of the stars, the observations made in the first two years are not sufficient to attempt any meaning light curve construction ; some of the data are too fragmented and others show large scatter.

• 전기학회논문지
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• 제65권8호
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• pp.1326-1333
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• 2016

It is important to clear the fault and prevent resulting in damage to power system. Although the frequency of generator internal fault is relatively low, it can lead to incalculable damage to power system as well as generator. Especially, loss of field on generator can cause the generator to lose synchronism for a short time if it is not removed promptly. Therefore, it is needed to conduct research on loss of field relay for detecting or clearing the loss of field. However, the setting of the relay may vary in generator operator or engineer, and the relay is not coordinated well with other elements associated with loss of field. In this paper, we address specifically the coordination of positive offset mho loss of field relay which is one of the protection schemes for loss of field. Computer simulations are performed by using ElectroMagnetic Transients Program-Restructured Version (EMTP-RV) based on actual data.

• 한국염색가공학회지
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• 제19권3호
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• pp.26-36
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• 2007

This study was carried out to treat the aqueous solutions containing reactive dyes(RB19, RR120 and RY179) by the Ozone demand flask method and adsorption process using activated carbon fiber(ACF) which are one of the main pollutants in dye wastewater. Ozone oxidation of three kinds of the reactive dyes was examined to investigate the reactivity of dyes with ozone, competition reaction and ozone utilization on various conditions for single- and multi-solute dye solution. Concentration of dyes was decreased continuously with increasing ozone dosage in the single-solute dye solutions. Competition quotient values were calculated to investigate the preferential oxidation of individual dyes in multi-solute dye solutions. Competition quotients(CQi) and values of the overall utilization efficiency, ${\eta}O_3$, were increased at 40mg/l of ozone dosage in multi-solute dye solutions. ACF(A-15) has much larger specific surface area$(1,584m^2/g-ACF)$ in comparison with granular activated carbon adsorbent (F400, $1,125m^2/g-GAC$), which is commonly used, and most of pores were found to be micropores with pore radius of 2nm and below. It was found that RB19 was most easily adsorbed among the dyes in this study. In the case of PCP (p-chlorophenol) and sucrose, which are single component adsorbate, adsorption capacities of ACF(A-15) were in good agreement with the batch adsorption measurement, and saturation time predicted of ACF columns for these components was also well agreed with practically measured time. But in the case of reactive dyes, which have relatively high molecular weight and aggregated with multi-components, adsorption capacities or saturation time predicted were not agreed with practically measured values.