• The Journal of the Acoustical Society of Korea
• /
• v.36 no.3
• /
• pp.172-178
• /
• 2017

Ultrasound has been widely used for biological and medical applications including induction of cell death, but a precise mechanism of induced cell death by ultrasound is controversial. In this study, an irradiation system with 40 kHz ultrasound was developed for a suitable cell death test of a representative unicellular organism, yeast, and used to study the biological effect of ultrasound on inducing cell death. Potassium Iodide (KI) dosimetry was used to devise an optimal system that successfully delivers 40 kHz ultrasound and produces reactive oxygen species in a 1.5 ml Eppendorf tube. Cell death was observed in an ultrasound transmission time-dependent fashion in this system. Thermal effect during irradiation was not observable in ultrasound induced cell death. Co-treatment of 40 kHz ultrasound and hydrogen peroxide showed a synergistic effect in inducing cell death. This finding suggests that 40 kHz ultrasound is related to reactive oxygen species formation. However, NAC (N-acetyl-L-cysteine) oxygen scavenger slightly inhibited the cell death by 40 kHz ultrasound. It was also found that 40 kHz ultrasound induced cell death was slightly inhibited by inhibitors of necrosis or apoptosis (glycyrrhizin or zVAD-fmk). This study suggests that cell death induced by 40 kHz ultrasound may not be exclusively related to reactive oxygen species formation and thermal effects in irradiated yeast cells.

• Korean Journal of Veterinary Research
• /
• v.35 no.2
• /
• pp.237-243
• /
• 1995

The effects of various autonomic blocking agents to perivascular nerve stimulation were investigated on isolated coronary artery of pig. 1. The magnitude of contractile response to perivascular nerve stimulation increased with increasing frequency(280Hz) of stimulation. 2. The contractions to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) were increased by pretreatment of the cholinestrase inhibitor, physostigmine. 3. The contraction to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) was antagonised by the muscarinic antagonist, atropine. 4. The contraction to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) was blocked by the neural blocker, tetrodotoxin. 5. The contractions to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) were not significantly affected by the ${\alpha}$-adrenergic antagonist, phentolamine or ${\beta}$-adrenergic antagonist, propranolol. 6. The contractile response by the acetylcholine was increased by the pretreatment of cholinestrase inhibitor, physostigmine. This findings suggest that the powerful excitatory action by the perivascular nerve stimulation may be linked to muscarinic receptor by cholinergic nerve excitation in coronary artery of pig.

• Proceedings of the IEEK Conference
• /
• 1998.10a
• /
• pp.605-608
• /
• 1998

In this paper, we have investigated the pyroelectric characteristics of the $20\mu\textrm{m}-thick$ $LiTaO_3$ single crystal with black coating by using the nondestructive dynamic method. The $LiTaO_3shows$ the maximum pyroelectric coefficient (${\gamma}$) of $1.56$\times$10-8C/\textrm{cm}^2K$ at 40Hz and the responsivity (Rv) is 488V/W at 2Hz. The noise equivalent power (NEP) is obtained as 3.95$\times$10-10W/√Hz at 40Hz. The detectivity (D*) is obtained divided by the sample area and estimated to be 5.6$\times$108cm√Hz/W at 40Hz. These results, shows that the $LiTaO_3$ single crystals are the best candidates to pyroelectric IR sensors.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.37 no.3
• /
• pp.189-195
• /
• 2001

The characteristics of echolocation signals of the Common Dolphin, Delphinus Delphis was observed by the hydrophone in order to detect exactly distribution and migration on whales and dolphins in Korean Coastal waters. It's observation was carried out at the position of 13 mm off Gam-Po of Korean east-southern sea at 3rd-5th. April and 13th-15th. October, 1999. The results obtained are summarized as follows: (1) The frequency range of ship's noise and ambient noise in the observed station was 0.5-0.3 kHz, that ones could be influenced to the behavior of common dolphins which carry out echolocation using low-frequency. (2) The common dolphin was radiated single click of 8.6 ms and double click of 4.8 ms pulse width during these observation (3) The high click frequencies of common dolphin were 5.10 kHz, 7.22 kHz, 10.60 kHz with the click pulse width of 4.0 ms, 2.6 ms, 1.0 ms, respectively. In case of low-frequency 1-2 kHz, that is, 1.12 kHz, 1.38 kHz, 1.82 kHz, pulse width were 22.4 ms, 2.05 ms, 11.9 ms, respectively and they showed a tendency using triple click signal. (4) The pulse width, pulse recurrence interval and frequency range of the observed echolocation signals were 2.4-8.4 ms, 9.0-40.0 ms, 0.60-10.63 kHz respectively, and frequency spectrum level was 100-125 dB for single, double, triple click signals.

• Journal of the Microelectronics and Packaging Society
• /
• v.9 no.1
• /
• pp.1-7
• /
• 2002

The purpose of this work is to develop High-Frequency Active Filter and super-miniaturation technology(SMD Type) of Filter which are essential for the key R/F Microwave components in the Mobile telecommunication system. The cut-off frequency of high frequency active filter for multimedia is 2.5 MHz, the gain is 0.5dB at 100 kHz, the passband ripple is 1.2 dB max at 100 kHz~2.0 kHz, GDT is 60 nsec at 100 kHz-2.0 MHz, the attenuation is 40 dB min at 3.75 MHz.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.12 no.2
• /
• pp.55-61
• /
• 2013

The horn is a key part of the ultrasonic welder. As the shape, mass and material of a horn have effects on the resonant frequency and the vibration mode in ultrasonic welding, a horn has to be designed and manufactured accurately. In this study, 40kHz band horn was designed and manufactured through the vibration mode and finite element analysis. A result of modal analysis showed that the natural frequency of the horn was 39,794Hz, and the frequency response by a harmonic response analysis was 39,800Hz - close to the intended frequency, 40kHz. In addition, weldability of the developed horn was estimated by welding of two Ni sheets and tensile-shear test of welded samples. It was shown the developed horn could be used in metal sheet welding.

• Journal of Korean Society of Environmental Engineers
• /
• v.28 no.2
• /
• pp.207-215
• /
• 2006

This study evaluated the effect of ultrasonic irradiation on reducing membrane fouling for the treatment of chemically treated leachate and biologically treated leachate. The experiments on improvement of membrane flux according to the membrane types(MF and UF) and membrane materials were performed with changing frequency(40 kHz and 120 kHz) and intensity(200 W and 400 W) of ultrasound in ultrasonic membrane separation apparatus which ultrasound can be periodically irradiated. Additionally, the effect of dual frequency ultrasound which 40 kHz md 120 kHz are irradiated simultaneously was evaluated. The improvement of membrane flux by periodical ultrasound irradiation was higher in microfiltration(MF) membrane than in ultrafiltration(UF) membrane. It was sustained more in the MF membrane of polyvinylidene fluoride(PVDF) than in that of polysulfon(PS). Ultrasonic improvement of membrane flux was different depending on the characteristics of target wastewater. It was sustainably maintained without reclogging using dual frequency ultrasound although the improvement of membrane flux was lower.

• Journal of Sensor Science and Technology
• /
• v.11 no.3
• /
• pp.145-154
• /
• 2002

NiCr-heaters with three different thicknesses ranging from 400 nm to 800 nm were fabricated and their characteristics were compared for the purpose of developing a chromel-alumel multijunction thermal converter for low input voltage with low frequency. The thermoelectric effect-induced AC-DC voltage transfer difference of the thermal converter with a built-in NiCr-heater of 400 nm-thickness was ${\pm}0.51{\sim}1.69\;ppm$ in the DC reversing frequency of $40\;Hz{\sim}10\;kHz$ with appling $0.5\;V_{rms}$ and the difference was increased to ${\pm}40{\sim}{\pm}115\;ppm$ in the frequency of $40\;Hz{\sim}1\;MHz$, when both thermoelectric effects and frequency effects were considered, showing the thermal converter would be suitable for the low input voltage application with low frequency.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.10 no.6
• /
• pp.71-76
• /
• 2010

In this paper, a 120 GHz local oscillator(LO) for the sub-harmonic mixer in the THz transceiver with a carrier frequency of 240 GHz was designed and fabricated. A 120 GHz local oscillator was composed of 40 GHz PLL(Phase Locked Loop), 40 GHz BPF(Band Pass Filter), frequency tripler and 120 GHz BPF. The commercial model of the frequency tripler was used. The measured result of the 40 GHz PLL showed the phase noise of -105 dBc/Hz at the 100 kHz offset frequency. The measured result of 120 GHz BPF showed the insertion loss of 1.3 dB at center frequency of 119 GHz with bandwidth of 5 GHz. The output power of 120 GHz LO was measured to 6.6 dBm.

• 한국초전도학회:학술대회논문집
• /
• v.9
• /
• pp.27-32
• /
• 1999

We developed a 40-channel superconducting quantum interference device (SQUID) system for neuromagnetic measurements. The main features of the system are use of double relaxation oscillation SQUID (DROS), and planar gradiometer for measuring tangential field components. The DROSS with high flux-to-voltage transfers enabled direct readout of the SQUID output by room-temperature electronics and simple flux-locked loop circuits could be used for SQUID operation. The pickup coil is an integrated first-order planar gradiometer with a baseline of 40 mm. The average noise of the 40 channels is around 1.2 fT/cm/${\sqrt{Hz}}$ at 100 Hz, corresponding to the field noise of 5 fT/${\sqrt{Hz}}$ at 100 Hz, operated inside a magnetically shielded room. The 40-Channel system was applied to measure auditory-evoked neuromagnetic fields.