• Genomics & Informatics
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• 제4권3호
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• pp.97-102
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• 2006

In acute leukemia patients, several successful methods of expression profiling have been used for various purposes, i.e., to identify new disease class, to select a therapeutic target, or to predict chemo-sensitivity and clinical outcome. In the present study, we tested the peripheral blood of 47 acute leukemia patients in an attempt to identify differentially expressed genes in AML and ALL using a Korean-made 10K oligo-nucleotide microarray. Methods: Total RNA was prepared from peripheral blood and amplified for microarray experimentation. SAM (significant analysis of microarray) and PAM (prediction analysis of microarray) were used to select significant genes. The selected genes were tested for in a test group, independently of the training group. Results: We identified 345 differentially expressed genes that differentiated AML and ALL patients (FWER<0.05). Genes were selected using the training group (n=35) and tested for in the test group (n=12). Both training group and test group discriminated AML and ALL patients accurately. Genes that showed relatively high expression in AML patients were deoxynucleotidyl transferase, pre-B lymphocyte gene 3, B-cell linker, CD9 antigen, lymphoid enhancer-binding factor 1, CD79B antigen, and early B-cell factor. Genes highly expressed in ALL patients were annexin A 1, amyloid beta (A4) precursor protein, amyloid beta (A4) precursor-like protein 2, cathepsin C, lysozyme (renal amyloidosis), myeloperoxidase, and hematopoietic prostaglandin D2 synthase. Conclusion: This study provided genome wide molecular signatures of Korean acute leukemia patients, which clearly identify AML and ALL. Given with other reported signatures, these molecular signatures provide a means of achieving a molecular diagnosis in Korean acute leukemia patents.

• 펄프종이기술
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• 제46권4호
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• pp.1-10
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• 2014

In papermaking industry, inorganic fillers are widely used for the purpose of improving opacity, brightness, printability, uniformity and dimensional stability. They are also useful for production costs and energy savings. In the past, inorganic fillers in papermaking industry only focused on micro-scale but recently, new trials on nano-powdered technology are applying. Even nano-powdered fillers are rapidly utilized for improving the optical and surface properties in coating and surface sizing, there still have some problems in wet-end process due to poor dispersibility and retention. In this study, nano-particled calcium carbonate was produced by milling the PCC and its applicability between micro sized and nano sized calcium carbonated was compared in wet-end process, and finally the sheet properties were evaluated. Nano-PCC was not retained in sheet structure without applying retention system, but with retention system nano-powdered PCC was absorbed on fiber surface with expanding the fiber networks. The application of PAM-bentonite system has resulted in high ash retention and bulky structure for copier paper, and good optical properties in brightness and opacity. However, it required to solve the weakness of low tensile property due to interruption of hydrogen bonding by nano fillers.

• 소성∙가공
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• 제29권4호
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• pp.203-210
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• 2020

In this study, formability and springback behavior of 1.5 GPa grade ultra-high strength steel (UHSS) sheet were predicted through the finite element simulation, and structural stability of the forming dies was verified by the coupled forming-structural analysis. Uniaxial tension and uniaxial tension-compression tests were performed to obtain experimental data for modeling the springback properties of the sheet material. The springback values predicted by simulation were compared with those from actual measurements. The results calculated from the kinematic hardening model were found to be much more accurate than those from the isotropic hardening model. Deformation of the forming die and springback of the product were calculated by the coupled forming-structural analysis. The higher the strength of the die material, the smaller the surface displacement of the die and the springback of the product. The internal stresses of the dies made of three materials, FC300, FCD550 and STD11 were compared with the yield stress of each material. The results provided a basis for determining the most suitable material for each part of the die set. As a result, simulation techniques have been established for predicting formability and springback in the UHSS sheet forming process.

• Fisheries and Aquatic Sciences
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• 제12권1호
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• pp.70-77
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• 2009

Porphyra yezoensis is known to act as a biofilter against nutrient-rich effluent in seaweed-based integrated aquaculture systems. However, few studies have examined its physiological status under such conditions. In this study, we estimated the photosynthetic activity of P. yezoensis by chlorophyll fluorescence of PSII (${\Delta}F/F'm$ and relative $ETR_{max}$) using the Diving-PAM fluorometer (Walz, Germany). In addition, bioremediation capacity, tissue nutrients, and C:N ratio of P. yezoensis were investigated. The ammonium concentration in seawater of seaweed tank 4 decreased from $72.1{\pm}2.2$ to $33.8{\pm}0.4{\mu}M$ after 24 hours. This indicates the potential role of P. yezoensis in removing around 43% of ammonium from the effluents. Tissue carbon contents in P. yezoensis were constant during the experimental period, while nitrogen contents had increased slightly by 24 hours. In comparison with the initial values, the ${\Delta}F/F'm$ and $rETR_{max}$ of P. yezoensis had increased by about 20 and 40%, respectively, after 24 hours. This indicates that P. yezoensis condition improved or remained constant. These results suggest that chlorophyll fluorescence is a powerful tool in evaluating the physiological status of seaweeds in a seaweed-based integrated aquaculture system.

• Macromolecular Research
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• 제15권7호
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• pp.688-692
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• 2007

The hydrogen-bonding induced alternating multilayer thin films of dendrimers and block copolymer micelles were demonstrated. The block copolymer micelles derived from amphiphilic poly(2-ethyl-2-oxazoline)block-$poly({\varepsilon}-carprolactone)$ (PEtOz-PCL) in aqueous phase have a core-shell structure with a mean hydrodynamic diameter of 26 nm. The hydrogen bonding between the PEtOz outer shell of micelle and the carboxyl unit of poly(amidoamine) dendrimer of generation 4.5 (PAMAM-4.5G) at pH 3 was utilized as a driving force for the layerby-layer alternating deposition. The multilayer thin film was fabricated on the poly(methyl methacrylate) (PMMA) thin film spin-coated on silicon wafer or glass substrate by the alternate dipping of PEtOz-PCL micelles and PAMAM dendrimers in aqueous solution at pH 3. The formation of multilayer thin film was characterized by using ellipsometry, UV-vis spectroscopy, and atomic force microscopy. The PEtOz outer shell of PEtOz-PCL micelle provided the pH-responsive hydrogen bonding sites with peripheral carboxylic acids of PAM AM dendrimer. The multilayer thin film was reversibly removed after dipping in aqueous solution at $pH{\geq}5.6$ due to dissociation of the hydrogen bonding between PEtOz shell of PEtOz-PCL micelle and peripheral carboxyl units of PAMAM dendrimer.

• Journal of Acupuncture Research
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• 제23권5호
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• pp.155-165
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• 2006

Objectives : This study was to examine the effect of Intradermal Acupuncture therapy at Kyeonjeong, Cheonryo and Kokji on the shoulder pain. The research was conducted with non equivalent control group non-synchronized design. Methods :　IThe subjects were the women who was suffering for shoulder pain, who are employed at two hospitals in Busan, from April to June, 2006. The 24 women were experimental group who were applied to Intradermal Acupuncture Therapy at Kyeonjeong, Cheonryo and Kokji, while the 20 women were control group who weren't applied to any treatments. The severity of shoulder pain and the extent of the subjective symptoms of shoulder pain were pre-measured. The Intradermal Acupuncture therapy was done a total of 4 times, 2 times a week over 2 weeks and from 11 a.ｍ. to 2 p.m. as an experimental treatment. As a post survey, the severity of shoulder pain, the frequency of shoulder pain and the extent of the subjective symptoms of shoulder pain were measured after one week and two weeks of experimental treatment. Research tools are Visual Analogue Scale by Cline et al. for measuring the severity of shoulder pain and the tool developed by Japan's industrial fatigue research committee of the industrial hygienics society and modified. Results : There was a significant difference(F=4.132, p=0.019) for the severity of shoulder pain on the interaction between time and group. There was a significant difference(F=11.193, p=0.000) for the extent of the subjective symptoms of shoulder pain on the interaction between time and. group. Conclusion : Based on the above results, it can be determined that Intradermal Acupuncture Therapy at Kyeonjeong, Cheonryo and Kokji can be used as the effective treatments for reducing of shoulder pam.

• International Journal of Automotive Technology
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• 제6권2호
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• pp.149-159
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• 2005

A full nonlinear finite element P185/70Rl4 passenger car radial-ply tire model was developed and run on a 1.7-meter-diameter spinning test drum/cleat model at a constant speed of 50 km/h in order to investigate the tire transient response characteristics, i.e. the tire in-plane free vibration modes transmissibility. The virtual tire/drum finite element model was constructed and tested using the nonlinear finite element analysis software, PAM-SHOCK, a nonlinear finite element analysis code. The tire model was constructed in extreme detail with three-dimensional solid, layered membrane, and beam finite elements, incorporating over 18,000 nodes and 24 different types of materials. The reaction forces of the tire axle in vertical (Z axis) and longitudinal (X axis) directions were recorded when the tire rolled over a cleat on the drum, and then the FFT algorithm was applied to examine the transient response information in the frequency domain. The result showed that this PI 85/70Rl4 tire has clear peaks of 84 and 45 Hz transmissibility in the vertical and longitudinal directions. This result was validated against more than 10 previous studies by either theoretical or experimental approaches and showed excellent agreement. The tire's post-impact response was also investigated to verify the numerical convergence and computational stability of this FEA tire model and simulation strategy, the extraordinarily stable scenario was confirmed. The tire in-plane free vibration modes transmissibility was successfully detected. This approach was never before attempted in investigations of tire in-plane free vibration modes transmission phenomena; this work is believed to be the first of its kind.

• 한국해양바이오학회지
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• 제5권4호
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• pp.40-45
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• 2011

Microalgae are one of the major, sustaining components of ecosystem processes and are responsible for biogeochemical reactions that drive our climate changes. Despite this, many marine microalgae are poorly described and little is known of their abundance and distribution along the coastal areas of Sooyoung Bay, Busan, South Korea. The present study has been conducted from November, 2011 to August, 2009 with the objective to provide an overview of the taxonomy diversity and abundance of microalgae along the coastal areas of the Sooyoung Bay. Water samples were collected from different sites, which were located by using a GPS tracker. Chlorophyll fluorescence of the water samples were measured by using ToxY-PAM dual-channel yield analyzer. The chlorophyll fluorescence values were relatively higher during the spring and summer and even in the region near to the sea port. Similarly the abundance of microalgae was higher near the port but diversity index had lower values. The temperature and pH values were same at all the sites. However, only the temperature varied during the sampling period, with higher values during summer and lower in winter. From the preliminary results, the following class of microalgae were found; Bacillariophyceae, Dinophyceae, Silicoflagellate and Cryptophyceae. With a future ongoing work, microalgae are being isolated to establish single cell culture and for identification using light microscopic observations, photography and molecular approaches.

• 대한물리치료과학회지
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• 제3권4호
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• pp.207-214
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• 1996

Main cause of low back pain is a poor posture. Most low back pains are due to a poor posture. The poor posture induces muscle tension and finaliy low back pain. The poor posture arehabitually trained from the childhood by the environmetal factors. In general, maintaining good posture during working and sleeping hours are the first line of defence against back pam. (1) Supine posture is the easiest posture that relaxes and fixes muscles. Supine posture is thus a starting position for on exercise. Lying down releases the weight pressures of head and shoulder and thus body can be relaxed and extended which are helpful for treating back pain. However, supine posture can increase the pressure in ribcage posture aspect and disphragram due to visceral oragans. (2) Sitting in one position for a long time results in fatigue and relaxation of spinal muscies. Finally, body strength is weakened and sitting posture will become poor. If this poor posture continues for a longer time, pain will be accompanied due to overelongation of muscle ligaments. The habitual poor posture could induce intervertebral disc distortion. If the intervertebral disc is damaged, sitting in one position or movement causes pain. (3) Abnormal lumbar curve induces the tention of abdominal muscle and paravertebral muscle groups as well as tention of lower limb muscle group connected to pelvis. For a person with weak body strength, muscle relaxation increases curvature in lumbar, chest and cervical regions. This will induce a pelvic anterior tilting of the imaginary line between A. S. I. S. and P. S. I. S. Hip joint extensor muscle acts on releasing the pelvic anterior tilting. Contrections of hamstring muscle and femoral muscle recover the imaginary line between A.S.LS. and P.S.I.S. from pelvic anterior tilting. thus, contraction of rectus abdominis muscle are required to maintain the normal lumbar curve.

• 한국안전학회지
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• 제29권6호
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• pp.166-171
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• 2014

Nuclear power plants are equipped with the reactor trip system (RTS) and the engineered safety features actuation system (ESFAS) to improve safety on the normal operation. In the event of the design basis accident (DBA), a various of post accident monitor(PAM)systems support to provide important details (e.g. Containment pressure, temperature and pressure of reactor cooling system and core exit temperature) to determine action of main control room (MCR). Operator should be immediately activated for the accident mitigation with the information. Especially, core exit temperature is a critical parameter because the operating mode converts from normal mode to emergency mode when the temperature of core exit reaches $649^{\circ}C$. In this study, uncertainty which was caused by exterior environment, characteristic of thermocouple/connector and accuracy of calibrator/indicator was evaluated in accordance with ANSI-ISA 67.04. The square root of the sum of square (SRSS) methodology for combining uncertainty terms that are random and independent was used in the synthesis. Every uncertainty that may exist in the hardware which is used to measure the core exit temperature was conservatively applied and the associative relation between the elements of uncertainty was considered simultaneously. As a result of uncertainty evaluation, the channel statistical allowance (CSA) of single channel of core exit temperature was +1.042%Span. The range of uncertainty, -0.35%Span ($-4.05^{\circ}C$) ~ +2.08%Span($24.25^{\circ}C$), was obtained as the operating criteria of core exit temperature.