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Changes in the Contents of Prosapogenin in the Skin White ginseng(Panax ginseng) Depending on Extracting Batches (피부백삼의 추출 조건에 따른 프로사포게닌 함량 변화)

  • Im, Byung Ok;Cho, Soon Hyun;Ko, Sung Kwon
    • Korean Journal of Pharmacognosy
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    • v.45 no.4
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    • pp.315-319
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    • 2014
  • This study compared the contents of ginseng prosapogenin depending on the extracting conditions of Skin White ginseng(Panax ginseng) to provide basic information for developing Skin White ginseng-based functional foods. Our findings show that the content of crude saponin peaked at 4 hours of extraction(SWG-4) and when extracted at $100^{\circ}C$. However, the content of total saponin reached its height at 8 hours of extraction at $100^{\circ}C$(SWG-8). On the other hand, the content of prosapogenin reached their heights at 60 hours of extraction(SWG-60), followed by 60 hours of extraction at $100^{\circ}C$. And at $100^{\circ}C$ the main prosapogenin of the content of Red and Black ginseng ginsenoside $Rg_3$, $Rg_5$ and $Rk_1$ reached their heights at 60 hours of extraction(SWG-60), followed by 60 hours of extraction.

Early Postoperative Hemodynamic Changes in Patients of Congenital Complex Heart Disease using Blood Cardioplegic Solution (Blood cardioplegic solution을 사용한 선천성 복잡 심기형 환자의 술 후 조기 혈류학 변화에 관한 연구)

  • Kim, Yeong-Tae;Kim, Yong-Jin
    • Journal of Chest Surgery
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    • v.25 no.11
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    • pp.1192-1202
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    • 1992
  • Postoperative cardiac outputs and other hernoaynamic values were serially measured in fifteen patients of cyanotic congenital heart disease, after use of blood cardioplegic solution. Cardiac indices showed no change untill eight postoparative hours, then it began to decline to reach as low as 3.22$\pm$0.7L/min/m2 at 12 hours. After then gradual increse occ-ured to recover upto immediate postoperative value at 20 hours. Sharp decrese of heart rate and increse of systemic vascular resistance during 8~16 hours and steady increase of stroke indices during the whole study periods were observed. These observations suggested that the myocardium recovered gradually after open heart surgery, and that the decrease of cardiac indices during 8~16 hours could be a result of decrease of heart rates and increase of afterload. The changes of cardiac indices correlated with the changes of heart rate, postoperative time and mixed venous oxygen saturation [p<0.05]. No other hemodynamic values found to be in statistically significant correlation with the changes of cardiac indices. Left ventricular dysfunction seemed to occur more frequently during 8~12 hours, but it was not statistically significant. [p=0.73] In conclusion, great care must be taken during 2~3 days after the operation of cyanotic congenital heart disease, not to fall into a low cardiac output state, by maintaing adequate heart rates and reducing afterload especially when the systemic vascular resistance increases.

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Studies on Antibiotic Residues in Cow Milk (우유(牛乳)의 잔류항생물질(殘溜抗生物質)에 관(關)한 연구(硏究))

  • Kim, Kyo Jun
    • Korean Journal of Veterinary Research
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    • v.13 no.1
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    • pp.57-62
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    • 1973
  • The antibiotics are usually used for the medical treatments or the other purpose for cow and almost of them are transfered to milk. Moreover a serious problems on public health are caused by misuse or overuse of antibiotics. These studies were conducted to determine the rise and fall of antibiotics in the raw milk which is milked from the cow treated with antibiotics. The number of samples examined were 500 which were taken from 36 cows, and the antibiotics in milk were detected by cylinder plate assay method. The results were as follows: 1) When penicillin, chloramphenycol and streptomycin were infused into a udder, they were detected up to the period of 84 hours, 36 hours and 60 hours after injection in the milk from the same udder, however in the milk from the other udder streptomycin was not detected but penicillin and chloramphenycol were detected up to 72 hours and 24 hours after injection. When streptomycin and penicillin were injected intramusculary, streptomycin was not detected in the milk but penicillin was detected up to 60 hours after injcetion. 2) When penicillin, streptomycin and chloramphenycol were infused into the udder, the lesser amount of milk was produced, the more antibiotics were detected in the milk. 3) It is necessary policy for the public health to inhibit to be sold the milk up to 3 days or more after the injection on antibiotics or to add food blue to mastitis drugs.

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A Comparison of Minilaparotomy and Laparoscopic Sterilization (Minilaparotomy 불임술(不妊術)과 복강경불임술(腹腔鏡不妊術)에 관(關)한 비교연구(比較硏究))

  • Bai, Byoung-Choo
    • Clinical and Experimental Reproductive Medicine
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    • v.4 no.1
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    • pp.17-25
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    • 1977
  • Anderson(1937), Power and Barnes(1941) reported a study concerning a method of tubal sterilization in association with peritoneoscopy or laparoscopy in which they cauterized the tubes. There appears to have been a hiatus of interest in sterilization (cold or hot) associated with laparoscopy until reintroduction by Palmer(1963), Frangenheim(1964) and Steptoe(1967). On the other hand, for interval female sterilization, however, minilaparotomy is relatively new. By Saunder and Munsick(1972), John Lyle(1974), Frank Stubb(1974), Vitoon(1973) and B.C. Bai(1975), their own technique for interval female sterilization requires 2.0 to 2.5cm, incision at the margin of the mons pubis. In Korea, female sterilization by means of minilaparotomy firstly reported by B.C. Bai using Bai's uterine elevator, of his own device, early in 1975. Recently inteval female sterilization by laparoscopy and minilaparotomy are widely accepted throughout the world especially in Asian countries. Minilaparotomy is carried out from 1974, laparoscopic sterilization from 1976, and in this study each of 250 cases of those were analysed and discussed for the comparison at Seoul Red Cross Hospital. (1) In the age distribution, numerous clients were in their age of $31{\sim}35$ in laparoscopy as well as minilaparotomy. Average 33.7 years in L and 33.2 years in M. (M=minilaparotomy, L=laparoscopic sterilization) (2) As regarding living children, women having 3 children represented the greatest number, 113 cases out of 250 in M group and 102 cases out of 250 in L group. Average No. of child are 2.9 in Land 3.1 in M. (3) Concidering the operation day in the menstrml cycle, the greatest number of cases, those who underwent tubal sterilization during the days of $26{\sim}$, next during the $6{\sim}10$ days of the cycle in both group. (4) Concidering the operation time, 188 cases by laparoscopy were performed in $6{\sim}10$ minutes, 33 cases within 5 minutes and 24 cases in $11{\sim}15$ minutes. Maximum 50 minutes, minimum 4 minutes and average 8.3 minutes. The majority of cases (154 cases) by minilaparotomy required $6{\sim}10$ minutes and 67 cases $11{\sim}15$ minutes, 6 cases within 5 minutes. Maximum 30 minutes, minimum 4 minutes and average 10.4, minutes. In both groups, most of the reasons for the extra length were surgical difficulties such as thick abdominal wall, pelvic adhesion, less cooperation of patients in early period of this study. (5) Hospital stay after operation in L group required $3{\sim}4$ hours in 125 cases, $2{\sim}3$ hours in 41 cases, $4{\sim}5$ hours in 32 cases out of 250. Maximum 8 hours, minimum 1 hour and average 3.8 hours. In M group hospital stay required $6{\sim}7$ hours in 100 cases, over 7 hours in 85 cases, $5{\sim}6$ hours in 46 cases and so on. Maximum 14 hours, minimum 2 hours and average 6.5 hours. (6) The time between operation and gas passing in the majority cases of both groups, were $12{\sim}36$ hours. A veragetime 20.3 hours in L and 27.2 in M. (7) Laparoscopic sterilization coincident with induced abortion were carried out in 27 cases, laparoscopy with minilaparotomy to control for mesosalpingeal hemorrhage in 1 case. Minilaparotomy coincident with induced abortion were performed in 65 cases, D and C whit polypectomy, menstrual regulatian, and remaval of IUD in 1 case respectively. (8) In L group, 1 case of mesosalpingeal hemorrhage, 1 case of abdominal wall infection were complicated during operation. In M group, 1 case of uterine perfaration, 1 case of abdominal wall infection, 1 case of hemorrhage from omentum and 1 case of bloody vaginal discharge were complicated. No intensive medical treatment was required for those minor complications in both groups. (9) No failure has been recognized and these two sterilization techniques might be the simple, safe and the most effective method for permanent contraception at present time. There is no significant clinical defference between L and M group in this study.

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Characteristics and Yield of Jochung Processed by Different Preparation Methods (제조 방법에 따른 쌀 조청의 특성 및 수율)

  • Choi, Yoon-Hee;Baek, Ji-Eun;Park, Shin-Young;Choi, Hye-Sun;Song, Jin
    • The Korean Journal of Food And Nutrition
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    • v.27 no.3
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    • pp.414-420
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    • 2014
  • This study was performed to increase the yield and to reduce the processing times for the preparation to improve the productivity and quality of rice jochung, a traditional food in Korea. In order to evaluate the quality characteristics and yield of jochung, the viscosity, color value, mineral contents and the sensory evaluation were measured. Jochung is prepared from steamed rice (STR), wet-milled rice flour (WRF) and dry-milled rice flour (DRF) by processing methods of rice and reacting times (6 hours or 13 hours) of liquefaction and saccharification. There is commonly added liquefying enzyme for rice liquefaction (0.4%/10 kg rice, at $85{\sim}90^{\circ}C$ for 1 hour or 4 hours) and saccharogenic enzyme with malt (2.5% or 4.5%/10 kg rice, at $56{\sim}60^{\circ}C$ for 5 hours or 9 hours). The inner structural properties of WRF showed the more distinct shape regular structure of uncombined starch particles but the DRF closely maintained particles of rice flour observed by SEM. If processing times for liquefation and saccharification were reduced from 13 hours to 6 hours, the yield of jochungs prepared with WRF increased 8%, the DRF 7%, and the STR 3% respectively and the sensory evaluation as well as color values and overall desirability received high scores. The viscosity, color a and b values of jochung processed with WRF for 6 hours were lower than that processed for 13 hours. The viscosity and color a, b value and Ca content were decreased in the jochung processed with WRF or DRF for 6 hours, but Mg, P and K were increased than that of STR. Jochung processed by 0.4% liquefying enzyme and 2.5% malt with WRF for 6 hours will increase the yield, save manufacturing times and costs and will thereby enable cost-effective techniques.

Synthesis of Aluminum Nitride Powder from Aluminum Hydroxide by Carbothermal Reduction-Nitridation (알루미나 수화물로부터 탄소환원질화법에 의한 질화알루미늄 분말의 합성)

  • 황진명;정원중;최상욱
    • Journal of the Korean Ceramic Society
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    • v.31 no.8
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    • pp.893-901
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    • 1994
  • In this study, AlN powder of fine particle size and of high purity was synthesized by the carbothermal reduction-nitridation of monodisperse, spherical Al(OH)3 which had been prepared by sol-gel method using Al(O-sec-C4H9)3 as the starting material. Depending on the mixing order and kinds of reducing agents, the optimum condition for the preparation of AlN was determined as follows. AlN single-phase was produced by the carbothermal reduction-nitridation of (1) Benzene-washed Al(OH)3 and the reducing agent, carbon, which was mixed in a ball mill: for 5 hours at 140$0^{\circ}C$ under NH3 atmosphere; (2) The mixture prepared by hydrolysis of alkoxide solution into which carbon had been dispersed beforehand: for 5 hours at 135$0^{\circ}C$ ; (3) Al(OH)3 Poly(furfuryl alcohol) composite powder: for 2.5 hours at 135$0^{\circ}C$; (4) The mixture of Al(OH)3 and polyacrylonitrile: for 5 hours at 140$0^{\circ}C$. Addition of CaF2 increased the nitridation rate when carbon or polyacrylonitrile was used as the reducing agent; but it had no effect on the nitridation rate when furfuryl alcohol was used as the reducing agent.

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Studies on the Production of Lipase by Rhizopus delemar by (Rhizopus delemar의 Lipase 생산에 관한 연구)

  • 배정설;배국웅
    • Microbiology and Biotechnology Letters
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    • v.3 no.1
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    • pp.1-6
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    • 1975
  • The excellent strain $K_{52}$ for producing lipase was selected among 215 strains of Rhizopus sp. isolated from soil and other natural sources. The results investigated of microbiological characters and conditions for producing lipase Ivere summarized as follows: (1) Strain $K_{52}$ was similar to Rhizopus delemar in microbiological character. (2) The lipase activity was most vigorous after 48 hours in wheat bran culture, 96 hours in surface culture and 72 hours in shaking colure. (3) Surface culture was more suitable than in shaking culture for producing lipase. (4) In the case of wheat bran culture, producing of lipase was vigorous after 48 hours of culure period (3,800u/g) . (5) The optimum temperature for producing lipase was 3$0^{\circ}C$ both in wheat bran culture and in surfase culture.

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The Effect of NEES on the Occurrence of Caspase-3 in the Cerebellum of Rats with Transient Global Ischemia

  • Lee, Jung Sook;Song, Young Wha;Kim, Sung Won
    • Journal of International Academy of Physical Therapy Research
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    • v.5 no.2
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    • pp.718-722
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    • 2014
  • The cerebellum is known to control balance, equilibrium, and muscle tone. If the cerebellum becomes damaged, the body is unable to retain its balancing functions or involuntary muscle movement. This is why, in stroke patients, there is a high risk of functional disability, as well as a myriad of other disabilities secondary to stroke. Ischemia was induced in SD mice by occluding the common carotid artery for 5 minutes, after which blood was reperfused. Needle electrode electrical stimulation(NEES) was applied to acupuncture points, at 12, 24, and 48 hours post-ischemia on the joksamri. Protein expression was investigated through caspase-3 antibody immuno-reactive cells in the cerebral nerve cells and Western blotting. The results were as follows: The number of caspase-3 reactive cells in the corpus cerebellum 12 and 24 hours post-ischemia was significantly (p<.05) smaller in the NEES group compared to the GI group. caspase-3 expression 12 and 24 hours post-ischemia was significantly(p<.05) smaller in the NEES group compared to the GI group. Based on these results, NEES seems to have a significant effect on Caspase-3 in the cerebellum in an ischemic state at 12 and 24 hours post ischemia, NEES delays the occurrence of early stage apoptosis-inducing Caspase-3, delaying and inhibiting apoptosis. Further systematic studies will have to be conducted in relation to the application of this study's results on stroke patients.

Formation Behaviour Al-Ti Intermetallic Compounds by Ball milling methods (볼 밀링에 의한 Al-Ti 계 금속간화합물 생성 거동)

  • 안인섭
    • Journal of Powder Materials
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    • v.6 no.1
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    • pp.42-48
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    • 1999
  • Three mixtures of elemental powders of Al-25at.%Ti, 48at.%Ti and 70at.%Ti were offered to ball milling process for the formation of intermetallic compounds of $Al_3Ti$, AlTi and $Ti_3Al$. Ballmilling or attrition process were carried out at the condition of rotaing speed of 110 or 350 rpm at $10^{-3}$ torr vacuum or argon atmospheres. $Al_3Ti$phases were fully obtained by heat treatment for 1 hors at $600^{\circ}C$ with Al-25at.%Ti composition mixtures milled by 100 hours. The amorphous phase was completely formed at the composition of Al-48at.%Ti mixed powders by milling 100hours at the 50 to 1 weight ratio of ball to powder, and AlTi compounds were obtained by heat treament. In the case of Al-70at%Ti mixed powders milled for 100 hours, $Ti_3Al$ and $Al_3Ti$intermetallic compounds were formed by heat treatment for 1 hour at $600^{\circ}C$. By attrition milling of 350rpm for 10 hours, $Ti_3Al$ phase was formed completley after heat treatment for 1 hour at $600^{\circ}C$.

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Physicochemical Properties of Fermented Milk Supplemented with Helianthus tuberosus Powder (돼지감자 분말 첨가 발효유의 이화학적 특성)

  • Park, Byung Bae;Renchinkhand, Gereltuya;Nam, Myoung Soo
    • Journal of Dairy Science and Biotechnology
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    • v.37 no.3
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    • pp.196-205
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    • 2019
  • Helianthus tuberosus is a perennial plant in the genus, Asteraceae. Pork potato has various pharmacological functions such as improving constipation, preventing bowel disease, inhibiting colon cancer, reducing serum cholesterol, lowering blood lipids, and enhancing blood sugar. This study investigated the physicochemical properties of fermented milk by adding Helianthus tuberosus powder. During the fermentation process, the pH of the fermented milk with added Helianthus tuberosus was higher after 16 hours of fermentation. At 48 hours of fermentation, the pH decreased to 3.70, 3.65, 3.63, and 3.59 with 0% (the control group), 1%, 3%, and 5% Helianthus tuberosus added, respectively. In the last 48 hours of fermentation, the acidity increased to 2.35%, 2.57%, and 3.17% with 0% (the control group), 1%, 3%, and 5% Helianthus tuberosus added, respectively. The number of lactic acid bacteria increased as the quantity of Helianthus tuberosus added increased. The highest number of lactic acid bacteria was 9.96 log CFU/g after 16 hours of fermentation with a 5% addition of Helianthus tuberosus . After 48 hours of fermentation, the amount of lactic acid bacteria decreased to 7.84 log CFU/g and 7.88 log CFU/g in the control group and the 1% added Helianthus tuberosus group, respectively. The addition of 3% and 5% Helianthus tuberosus increased the lactic acid bacteria count to 9.48 log CFU/ g and 9.81 log CFU/g, respectively. As the fermentation time increased, oxalic acid and tartaric acid decreased but lactic acid increased. Lactose degraded galactose and glucose over time. After 48 hours of fermentation, the viscosity in the control, 1%, 3%, 5% added Helianthus tuberosus groups increased to 1,006 cP, 1,026 cP, 1,040 cP, and 1,106 cP, respectively. The antioxidant effect was higher in the 5% added Helianthus tuberosus group (84.14%) than in the control group (80.39%) at 48 hours of fermentation. The concentration of polyphenol was 1.6 mg/g and antimicrobial activity was strong against E. coli and Staphylococcus aureus.