• YAKHAK HOEJI
• /
• v.29 no.2
• /
• pp.107-109
• /
• 1985

A novel synthesis of 7-membered 1,3,5-benzotriazepine (IV) from 1,3-Dimethyl-5-azauracil in one step by s-Triazine ring transformation is reported.

• Journal of Microbiology and Biotechnology
• /
• v.9 no.2
• /
• pp.196-200
• /
• 1999

A recombinant human $\alpha_{s1}$-casein was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor $\alpha$l (MF$\alpha$l), inulinase, and human $\alpha_{s1}$-casein were used to direct the secretion of human $\alpha_{s1}$-casein into the extracellular medium. Among the three leader sequences tested, the native leader sequence of human $\alpha_{s1}$-casein was found to be the most efficient in the secretory expression of human $\alpha_{s1}$-casein, which implies that the native leader sequence of human $\alpha_{s1}$-casein might be used very efficiently for the secretory production of other heterologous proteins in yeast. The recombinant human $\alpha_{s1}$-casein was proteolytically cleaved as the culture proceeded. Therefore, an attempt was made to produce human $\alpha_{s1}$-casein using a S. cerevisiae mutant in which the YAP3 gene encoding yeast aspartic protease 3 (YAP3) was disrupted. After 72 h of culture, most of the human $\alpha_{s1}$-casein secreted by the wild type was cleaved, whereas more than 70% of the human $\alpha_{s1}$-casein secreted by yap3-disruptant remained intact. The results suggest that YAP3 might be involved in the internal cleavage of human $\alpha_{s1}$-casein expressed in yeast

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.37 no.5
• /
• pp.397-404
• /
• 1992

The mitotic cycle duration(MCD) and component phase periods of rice(Oryza sativa L.) root meristem cells on the different salt concentrations were investigated by using of tritiated thymidine. The time interval between the maxima of sequential mitotic appearances of marked cells was used as criteria in measuring the MCD of rice. The MCD of rice cultivars 'Seomjinbyeo and Chilseongbyeo' at 0.0%, 0.3%, and 0.6% of salt concentrations appeared the same period as 12hr. The durations of component phase of rice cultivar 'Seomjinbyeo' were the almost same periods at 0.1%, 0.3%, and 0.6% of salt, but in 'Chilseongbyeo' cultivar the G1 and G2 periods were shorter while the S period was longer at 0.3% and 0.6% of salt. Deoxyribonucleic acid(DNA) and protein synthesis were increased while ribonucleic acid(RNA) synthesis was decreased with increasing salt concentrations at Chilseombyeo roots. In Seomjinbyeo roots, DNA and RNA synthesis were decreased while protein synthesis was increased with increasing salt concentrations. These results suggest that DNA, RNA, and protein synthesis may not affect the MCD in rice, but the increase of protein synthesis may be related to the salt tolerance of rice.

• Horticultural Science & Technology
• /
• v.19 no.4
• /
• pp.501-504
• /
• 2001

The effect of soil EC on tissue morphology of leaf and shoot tip in cucumber (Cucumis sativus L. cv. Euinchim-baekdadagi) seedlings was investigated. Number of trichomes on leaf upper epidermis increased with the increase in soil EC from 1.0 to $3.0dS{\cdot}m^{-1}$, but the shape and number of stomata on lower epidermis remained unchanged. Epidermal cells of cucumbers grown in EC $1.5dS{\cdot}m^{-1}$ soil was occupied mostly by large vacuole whereas those grown in EC $3.0dS{\cdot}m^{-1}$ soil were filled with a nucleus, mitochondria, chloroplast and other micro-organelles. Sponge parenchima cells were also larger and contained fewer chloroplasts at EC $1.5dS{\cdot}m^{-1}$ than those grown at EC $3.0dS{\cdot}m^{-1}$. Leaf thickness decreased at high EC and the color of epidermal cells became significantly darker on the photograph of optical microscope. Normal tissue differentiation was greatly suppressed in plants grown in soils with $3.5dS{\cdot}m^{-1}$ or higher EC.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.29 no.1
• /
• pp.84-88
• /
• 1984

To obtain basic information on the breeding of early maturing, short plant height rapeseed varieties, the following 7 varieties, Isuzu, Miyuki, Norin 25, Rang, Yongdang, Cresus and Tower were used in diallel crosses in 1979. Maturing date, plant height and grain weight per plant for the parents, $F_1$'s and $F_2$'s of the 7 x 7 partial diallel crosses were measured in 1981 for analysis of their genetic behavior. The results obtained are summarized as follows: 1. The days to maturing of $F_1$'s showed complete dominance for early maturing, and both additive and dominance genetic variances were significant. Number of effective factors in $F_1$'s was 3, but in $F_2$'s was 1. The degree of dominance in $F_1$'s was partial, while in $F_2$'s was complete. Both broad and narrow sense herita-bilities in $F_1$'s was high, while in $F_2$'s was low. 2. Yield per plant in $F_2$'s was controlled by additive component of genetic variance only, but $F_1$'s was different. The degree of dominance in $F_1$'s was complete, while in $F_2$'s was partial. The direction of dominance showed almost complete dominance over high yield and three effective factors was estimated. Yield per plant was controlled by recessive genes. 3. The plant height was controlled by both dominance and additive variance. Dominance was directed toward tall plant height. Number of effective factors was 2, and broad and narrow sense heritability were high in the plant height.

• Journal of applied mathematics & informatics
• /
• v.39 no.1_2
• /
• pp.223-237
• /
• 2021

Let G = (V (G), E(G)) be a graph and let g : V (G) → S3 be a function. For each edge xy assign the label r where r is the remainder when o(g(x)) is divided by o(g(y)) or o(g(y)) is divided by o(g(x)) according as o(g(x)) ≥ o(g(y)) or o(g(y)) ≥ o(g(x)). The function g is called a group S3 cordial remainder labeling of G if |vg(i)-vg(j)| ≤ 1 and |eg(1)-eg(0)| ≤ 1, where vg(j) denotes the number of vertices labeled with j and eg(i) denotes the number of edges labeled with i (i = 0, 1). A graph G which admits a group S3 cordial remainder labeling is called a group S3 cordial remainder graph. In this paper, we prove that square of the path, duplication of a vertex by a new edge in path and cycle graphs, duplication of an edge by a new vertex in path and cycle graphs and total graph of cycle and path graphs admit a group S3 cordial remainder labeling.

• Ecology and Resilient Infrastructure
• /
• v.2 no.2
• /
• pp.154-160
• /
• 2015

This paper presents the field investigation of deposition and erosion relief of a riverfront using vegetation. The results obtained were as follows: Phragmites japonica showed 0.2 m of deposition and 0.3 m-0.4 m of erosion relief of river front by the critical velocity of 1.0 m/s-1.2 m/s. P. communis showed 0.1 m-0.4 m of deposition and 0.2 m-0.3 m of erosion relief by the critical velocity of 0.6 m/s-0.7 m/s. Salix gracilistyla showed 0.1 m-0.2 m of deposition and 0.4 m-0.5 m of erosion relief by the critical velocity of 1.2 m/s-1.4 m/s. Miscanthus sacchariflorus showed 0.1 m-0.4 m of deposition and 0.1 m-0.2 m of erosion relief by the critical velocity of 0.6 m/s-0.7 m/s. S. gracilistyla had the greatest role, while M. sacchariflorus had the lowest role for erosion relief. These results showed that aquatic plants had an effective role in sustaining a stable channel.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2008.05a
• /
• pp.23-25
• /
• 2008

Serum complement proteins comprise an important system that is responsible for several innate and adaptive immune defence mechanisms. There were three well described pathways known to lead to the generation of a C3 convertase, which catalyses the proteolysis of complement component C3, and leads to the formation of C3 opsonins (C3b, iC3b and C3d) that fix to bacteria. A pivotal step in the complement pathway is the assembly of a C3 convertase, which digests the C3 complement component to form microbial-binding C3 fragments recognized by leukocytes. The spleen clears microorganisms from the blood. Individuals lacking this organ are more susceptible to Streptococcus pneumoniae. Innate resistance to S. pneumoniae has previously been shown to involve complement components C3 and C4, however this resistance has only a partial requirement for mediators of these three pathways, such as immunoglobulin, factor B and mannose-binding lectin. Therefore it was likely that spleen and complement system provide resistance against blood-borne S. pneumoniae infection through unknown mechanism. To better understand the mechanisms involved, we studied Specific intracellular adhesion molecule-grabbing nonintegrin (SIGN)-R1. SIGN-R1, is a C-type lectin that is expressed at high levels by spleen marginal-zone macrophages and lymph-node macrophages. SIGN-R1 has previously been shown to be the main receptor for bacterial dextrans, as well as for the capsular pneumococcal polysaccharide (CPS) of S. pneumoniae. We examined the specific role of this receptor in the activation of complement. Using a monoclonal antibody that selectively downregulates SIGN-R1 expression in vivo, we show that in response to S. pneumoniae or CPS, SIGN-R1 mediates the immediate proteolysis of C3 and fixation of C3 opsonins to S. pneumoniae or to marginal-zone macrophages that had taken up CPS. These data indicate that SIGN-R1 is largely responsible for the rapid C3 convertase formation induced by S. pneumoniae in the spleen of mice. Also, we found that SIGN-R1 directly binds C1q and that C3 fixation by SIGN-R1 requires C1q and C4 but not factor B or immunoglobulin. Traditionally C3 convertase can be formed by the classical C1q- and immunoglobulin-dependent pathway, the alternative factor-B-dependent pathway and the soluble mannose-binding lectin pathway. Furthermore Conditional SIGN-R1 knockout mice developed deficits in C3 catabolism when given S. pneumoniae or its capsular polysaccharide intravenously. There were marked reductions in proteolysis of serum C3, deposition of C3 on organisms within SIGN-$R1^+$ spleen macrophages, and formation of C3 ligands. The transmembrane lectin SIGN-R1 therefore contributes to innate resistance by an unusual C3 activation pathway. We propose that in the SIGN-R1 mediated complement activation pathway, after binding to polysaccharide, SIGN-R1 captures C1q. SIGN-R1 can then, in association with several other complement proteins including C4, lead to the formation of a C3 convertase and fixation of C3. Therefore, this new pathway for C3 fixation by SIGN-R1, which is unusual as it is a classical C1q-dependent pathway that does not require immuno globulin, contributes to innate immune resistance to certain encapsulated microorganisms.

• The Transactions of the Korean Institute of Electrical Engineers
• /
• v.41 no.1
• /
• pp.56-62
• /
• 1992

The GaAs bulk crystals are grown by the Synthesis Solute Diffusion(SSD) method and its properties are investigated. The crystal growth rate at optimum condition is 0.28 cm/day and their temperature dependence is R(T) = 2.92 x 10S04T exp(-1.548eV/kS1BTT) [cmS02T/day.K]. Etch pits density distribution along radial direction is order of 10S04TcmS0-2T and 10S03TcmS0-2T at the edge and middle of the wafers, respectively, and it increased exponentially along vertical direction of ingot. Moreover,it is uniformly distributed as order of 10S03TcmS0-2T in radial direction of In doped GaAs. The carrier concentration and mobilities are measured to 0.34-2.1 x 10S016T cmS0-3T and 2.3-3.3x10S03T cmS02T/V.sec, respectively.

• Natural Product Sciences
• /
• v.14 no.3
• /
• pp.161-166
• /
• 2008

A mixture of sixteen cerebrosides, which comprised four cerebroside molecular species (PL-1 ${\sim}$ PL-4) was separated from the roots of Paeonia lactiflora. The structures of cerebrosides were characterized as $1-O-{\beta}$-D-glucopyranosides of phytosphingosines, which comprised a common long-chain base, (2S,3S,4R,8E/Z)-2-amino-8-octadecene-1,3,4-triol with eight fatty acids or 2-hydroxy fatty acids of varying chain lengths ($C_{16}$, $C_{18}$, $C_{20-26}$) linked to the amino group. Aralia cerebroside and its 8Z isomer (PL-1), $1-O-{\beta}$-D-glucopyranosyl-(2S,3S, 4R,8E/Z)-2-[(2'R)-2'-hydroxytetracosanoylamino]-8-octadecene-1,3,4-triol (PL-2), $1-O-{\beta}$-D-glucopyranosyl-(2S,3S,4R, 8E/Z)-2-[(2'R)-2'-hydroxydocosanoylamino]-8-octadecene-1,3,4-triol (PL-3), and $1-O-{\beta}$-D-glucopyranosyl-(2S,3S,4R, 8E/Z)-2-[(2'R)-2'-hydroxytricosanoylamino]-8-octadecene-1,3,4-triol (PL-4) were identified as major components of these cerebroside molecular species. All the major cerebrosides were shown to be a mixture of geometrical isomers (8E and 8Z) of phytosphingosine-type glucocerebrosides possessing 2R-hydroxy fatty acids. In addition, three ${\beta}-sitosterol$ derivatives and adenosine were also separated. The structures of these isolates have been determined on the basis of chemical and spectroscopic evidence.