• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.137-141
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• 2005

Though many insecticides are commercially available, development of resistance, pest resurgence and effects on non-target organisms led to the search for alternate insect pest management (IPM) strategy based on larval growth and reproductive fitness. Reproductive potential of insects depends on its acquiring of vitellogenic competence which is under hormonal control. Exogenous application of analogues of JR (JHAs) and ecdysterone could derail normal development and reproduction in insects by manipulating an array of physiological processes. In the rice pest, brown planthopper, Nilaparvata lugens, JHA, hydroprene induced metathetely from the fifth (final) instar nymphs in an age-dependent manner. Day 0 of the final instar showed highest sensitivity to induce this abnormal development. Adults emerged from treated day 3 nymphs looked normal. Both the morphotypes were reproductively incompetent and showed partial to complete sterility. Pre-adult exposure of the ovarian tissue to hydroprene suppressed mitotic division of germinal cells and induced abnormalities in the later s1ages of growth and differentiation of ovary in N. lugens. More over the nymphal exposure to hydroprene inhibited patency changes of follicular epithelium and affected competence of the follicles for yolk sequestration. In the absence of ovarian growth and oocyte differentiation, germarium found disintegrated, trophic core regressed and terminal oocytes resorbed. Hydroprene exposure to newly ecdysed brachypterous females did not affect ovarian development and egg production. Proper larval-adult transition appeared as a. prerequisite for vitellogenic competence in N. lugens for which the ovarian tissues must be exposed to ecdysterone in the internal milieu devoid of JH.

• 재활간호학회지
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• 제9권2호
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• pp.161-165
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• 2006

Purpose: The purpose of this study was to investigate the relationship between the level of mastery and depression in chronic arthritis patients. Method: The subjects for this study were 100 patients registered in S University Hospital, and the period of data collection was from June 20, 2006 to August 30, 2006. Results: The cronbach's ${\alpha}$ of the research instruments were .70-.86. In data analysis, SPSSWIN 12.0 program was used for descriptive statistics. The results were as follows. 1) The range of total mastery scores was from 11 to 28 and the mean score of the depression in chronic arthritis patients was 17.88. 2) The range of total depression scores was from 20 to 72 and the mean score of the depression in chronic arthritis patients was 39.99. 3) The level of mastery was significant correlation with the level of depression in chronic arthritis patients(r=-.466, p<.01). Conclusion: Mastery had significant correlation with depression in patients who have chronic arthritis. Therefore, the strategy of nursing intervention which improve mastery must be developed for patients who have chronic arthritis.

• 보건교육건강증진학회지
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• 제26권4호
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• pp.63-70
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• 2009

Objectives: Work stress is associated with increased risk of fatigue. The purpose of this study was to identify the relationship of work stress and fatigue among Medical Insurance Review Nurses. Methods: The subjects of this study were 117 nurses in charge of medical insurance review. A structured questionnaires were employed to evaluate the nurses' sociodemographics, work stress and fatigue. The data were analysed through frequency, percentile, mean, standard deviation, Chronbach's $\alpha$, Pearson's correlation coefficient, and multiple regression. Results: Mean scores of work stress and fatigue of participants were 3.29 and 1.97 respectively. Work stress was correlated with fatigue significantly (r=.39). There were effects on fatigue by work performance satisfaction and the frequency of insurance request among sociodemographics. Job conflicts with doctors which was a domain of work stress was increased fatigue. Conclusion: The results of this study suggest that work performance satisfaction, the frequency of insurance request and job conflicts with doctors are significant predictors of fatigue. Thus, a strategy to enhance nurses' work environment and to reduce work stress through developing interpersonal relationship is recommended.

• 한국유기농업학회지
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• 제19권spc호
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• pp.291-294
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• 2011

We studies a model for management of pepper anthracnose based covering method and spraying system in field. 1. Among 82 organic fungicides, 42 materials showed most effective inhibition against mycelia growth of the Colletotrichum acutatum in vitro. 23 formulated biocontrol agents were chosen to control the disease from 42 biocontrol agents in greenhouse. In the end, five kinds (2 plant extracts, 2 biopesiticides, 1 Bordeaux mixture) were selected from 23 materials in the field. 2. The mulching materials of bed covering in fruit season were thin non-woven fabric sheet and black plastic. The use of a fabric sheet was reduced the spread of anthracnose as compared to the plastic covering. 3. The application with the chosen materials was reduced 34% of anthracnose for 7 times sprays to planting 70 days as compared to the untreated control. In yield, nonwoven fabric sheet with formulated biopesticides was increased 17% than black plastic. 4. This result indicated that the developed biocontrol strategy could be an effective and economic crop protection system in organic pepper cultivation field.

• 미생물학회지
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• 제46권2호
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• pp.134-139
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• 2010

전 세계 인구의 1/3을 감염시키고 있는 결핵균은 21세기에도 인류를 위협하는 병원균이다. 결핵균에 대항하는 Th1 면역 반응은 결핵균의 세포 내 성장을 제어하는 것으로 알려져 있으나, 이는 결핵균 감염을 자연 치유하는 수준에는 미치지 못한다. 본 연구에서는 C57BL/6 마우스에 병원성 결핵균인 H37Rv를 감염시켰을 때 숙주의 면역 반응에 대항하여 결핵균이 자신의 유전자 발현을 변화시킨다는 사실을 규명하기 위하여, 결핵균 유전자 중 16S rRNA, acr, fbpA, aceA, ahpC 등의 발현을 real-time RT-PCR을 이용하여 연구하였다. 16S rRNA의 copy number는 감염 후 30일까지 급격하게 증가하였는데 이는 CFU 측정 결과와 일치하고 있다. 결핵균 유전자 중 주된 항원으로 작용하는 유전자인 fbpA의 copy number를 CFU로 나눈 값으로 표현한 발현 양상은 감염 후 10일까지 증가하다가 감소되었다. Heat shock protein인 ${\alpha}$-crystallin을 coding하는 acr은 감염 후 지속적으로 높아졌으나, 산화적 스트레스 환경에서 발현되는 효소들인 ahpC와 aceA의 발현은 감염 후 20일 동안 높아졌다가 30일에는 약간 감소하였으나 비교적 높은 수준을 유지하였다. 이상의 결과는 결핵균이 숙주의 면역 반응이 개시되면 결핵균의 주된 항원 중 하나인 Ag85A를 코딩하는 유전자인 fbpA의 발현 수준을 낮춰 숙주의 Th1 먼역 반응이 낮아지도록 유도한다는 증거로 볼 수 있으며, 면역 반응이 활발해 짐에 따라 큰포식세포 내에서 산화적 스트레스로부터 균을 보호하기 위하여 ahpC와 aceA의 발현이 높아진 것으로 해석된다. 따라서 본 연구는 결핵균이 유전자 발현을 숙주의 면역 반응에 대항하여 스스로 변화시켜 적대적인 숙주 세포 내에서 살아 남을 수 있는 생존 전략을 구사한다는 가능성을 제시한다.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.321-334
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• 2017

Process surveillance within agricultural biogas plants (BGPs) was concurrently studied by high-throughput 16S rRNA gene amplicon sequencing and an optimized quantitative microscopic fingerprinting (QMF) technique. In contrast to 16S rRNA gene amplicons, digitalized microscopy is a rapid and cost-effective method that facilitates enumeration and morphological differentiation of the most significant groups of methanogens regarding their shape and characteristic autofluorescent factor 420. Moreover, the fluorescence signal mirrors cell vitality. In this study, four different BGPs were investigated. The results indicated stable process performance in the mesophilic BGPs and in the thermophilic reactor. Bacterial subcommunity characterization revealed significant differences between the four BGPs. Most remarkably, the genera Defluviitoga and Halocella dominated the thermophilic bacterial subcommunity, whereas members of another taxon, Syntrophaceticus, were found to be abundant in the mesophilic BGP. The domain Archaea was dominated by the genus Methanoculleus in all four BGPs, followed by Methanosaeta in BGP1 and BGP3. In contrast, Methanothermobacter members were highly abundant in the thermophilic BGP4. Furthermore, a high consistency between the sequencing approach and the QMF method was shown, especially for the thermophilic BGP. The differences elucidated that using this biphasic approach for mesophilic BGPs provided novel insights regarding disaggregated single cells of Methanosarcina and Methanosaeta species. Both dominated the archaeal subcommunity and replaced coccoid Methanoculleus members belonging to the same group of Methanomicrobiales that have been frequently observed in similar BGPs. This work demonstrates that combining QMF and 16S rRNA gene amplicon sequencing is a complementary strategy to describe archaeal community structures within biogas processes.

• 정보화정책
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• 제27권2호
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• pp.3-19
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• 2020

인공지능과 블록체인은 4차산업혁명을 이끌어 갈 핵심기술로 각광을 받으며 발전하고 있다. 그러나 아직까지 인공지능은 데이터 확보 및 검증, 결과값에 대한 근거 설명 등에서 한계를 보이고 있고 블록체인 역시 과다한 에너지 소모, 데이터 관리의 유연성 부족 등이 확산을 저해하는 단점으로 꼽히고 있다. 이에 본 연구는 상호보완적인 성격을 지닌 인공지능과 블록체인의 기술 및 산업 동향과 한계점, 그리고 이를 극복하기 위한 기술융합 방안을 분석하고 정책 개선방안을 제시하였다. 구체적으로 혁신정책 관점을 적용해 연구개발(R&D) 강화 측면에서 1) 국가 차원의 중장기 인공지능/블록체인 융합 연구 추진, 2) 블록체인 기반 인공지능 데이터 플랫폼 구축, 혁신 생태계 조성 측면에서 3) 산업별 인공지능/블록체인 융합 응용 발굴 지원, 4) 인공지능/블록체인 융합 비즈니스 모델 개발 스타트업 지원, 법제도 개선 측면에서 5) 규제 샌드박스 확대 적용, 6) 개인정보보호 관련 규제 정비를 제안하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권9호
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• pp.1341-1350
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• 2009

Dietary lipids are rapidly hydrolysed and biohydrogenated in the rumen resulting in meat and milk characterised by a high content of saturated fatty acids and low polyunsaturated fatty acids (PUFA), which contributes to increases in the risk of diseases including cardiovascular disease and cancer. There has been considerable interest in altering the fatty acid composition of ruminant products with the overall aim of improving the long-term health of consumers. Metabolism of dietary lipids in the rumen (lipolysis and biohydrogenation) is a major critical control point in determining the fatty acid composition of ruminant lipids. Our understanding of the pathways involved and metabolically important intermediates has advanced considerably in recent years. Advances in molecular microbial technology based on 16S rRNA genes have helped to further advance our knowledge of the key organisms responsible for ruminal lipid transformation. Attention has focused on ruminal biohydrogenation of lipids in forages, plant oils and oilseeds, fish oil, marine algae and fat supplements as important dietary strategies which impact on fatty acid composition of ruminant lipids. Forages, such as grass and legumes, are rich in omega-3 PUFA and are a useful natural strategy in improving nutritional value of ruminant products. Specifically this review targets two key areas in relation to forages: i) what is the fate of the lipid-rich plant chloroplast in the rumen and ii) the role of the enzyme polyphenol oxidase in red clover as a natural plant-based protection mechanism of dietary lipids in the rumen. The review also addresses major pathways and micro-organisms involved in lipolysis and biohydrogenation.

• Ocean and Polar Research
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• 제30권1호
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• pp.119-128
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• 2008

Japan's new Basic Ocean Law took effect in 20 July 2007. This law contains that 1) calls for the consolidation of eight government offices that previously worked separately on maritime issues; 2) establishes a basic plan for maritime matters, and; 3) creates a comprehensive maritime policy headquarters, run by the Prime Minister. The result is a structure for the integrated promotion of maritime policy. The Minister of Land, Infrastructure and Transport has been appointed to the newly established position of maritime minister. Japan has been in conflict with Korea and China over EEZ and territory, which has caused the country to turn to ocean. If Japan puts more emphasis on sea, it will be on a collision course with neighboring countries such as Korea, China, Russia, and Taiwan. Japan has been at odds with these countries; with Korea over Dokdo islets, with China over the Senkaku Islands and the East China Sea, where gas fields lie, with Taiwan over fishery rights in the East China Sea, with Russia over the Kuril Islands. Korea's position about the establishment of Japan's new Basic Ocean Law is followed: 1) expression of Korea's position in maritime resourcces of east china sea, 2) understand of strategy for maritime resources development and maritime delimitation in China and Japan, 3) a caution for extention of EEZ and maritime activities, 4)effective and comprehensive policy establishment, and strength in R&D, 5) construction of active and responsive system for maritime issues in neighbor country.

• Nuclear Engineering and Technology
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• 제27권4호
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• pp.571-581
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• 1995

원자력 발전소의 비상운전절차서는 작성단계부터 난점이 많이 있고, 작성된 절차서를 착인 및 검증하는 데에도 많은 어려움이 따른다 캐나다의 원자력 규제기관인 AECB에서도 원자력발전소의 운전 허가를 받는 데 있어 발전소 비상운전 절차서를 하나의 요구조건으로 삼고 있는데, 그 요구조건은 발전소 내외의 비상상황에 대처하기 위한 상세한 계획 및 절차를 개발하는 것이다. 본 보고서에서는 미국의 원자력 발전소 비상운전 계획을 참고하고 있는 캐나다 원자력 발전소용 비상운전절차 표준지침서를 검토하고, 캐나다 비상운전 절차서 들의 현재 경향을 알아보았으며, 캔두형 발전소들의 비상운전 절차를 사고의 범위(개별적인 사고의 종류 지정), 사고진단 방법(사건 대응적, 징후 대응적, 또는 이 두 가지 방법의흔합형), 절차서 형식 등을 중점으로 비교 검토하고, 각 절차서의 내용을 포괄적으로 종합하여 현재 건설중인 월성 원자력 발전소 2, 3, 4호기용 비상운전절차서의 기본방향을 징후 대응적절차서(사고진단이 안될 경우 사용)와 논리도표와 문장이 혼합된 형식의 사건파악적절차서로 이루어진 비상운전절차서로 제시하였다.