• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.88-94
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• 2008

Two sugar biosynthetic cassette plasm ids were used to direct the biosynthesis of a deoxyaminosugar. The pOTBP1 plasmid containing TDP-glucose synthase (desIII), TDP-glucose-4,6-dehydratase (desIV), and glycosyltransferase (desVII/desVIII) was constructed and transformed into S. venezuelae YJ003, a strain in which the entire gene cluster of desosamine biosynthesis is deleted. The expression plasmid pOTBP3 containing 4-aminotransferase (gerB) and 3,5-epimerase (orf9) was transformed again into S. venezuelae YJ003-OTBP1 to obtain S. venezuelae YJ003-OTBP3 for the production of 4-amino-4,6-dideoxy-L-glucose derivatives. The crude extracts obtained from S. venezuelae ATCC 15439, S. venezuelae YJ003, and S. venezuelae YJ003-OTBP3 were further analyzed by TLC, bioassay, HPLC, ESI/MS, LC/MS, and MS/MS. The results of our study clearly shows that S. venezuelae YJ003-OTBP3 constructs other new hybrid macrolide derivatives including 4-amino-4,6-dideoxy-L-glycosylated YC-17 (3, [M+ $Na^+$] m/z=464.5), methymycin (4, m/z=480.5), novamethymycin (6, m/z=496.5), and pikromycin (5, m/z=536.5) from a 12-membered ring aglycon (10-deoxymethynolide, 1) and a 14-membered ring aglycon (narbonolide, 2). These results suggest a successful engineering of a deoxysugar pathway to generate novel hybrid macrolide derivatives, including deoxyaminosugar.

• Analytical Science and Technology
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• v.36 no.3
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• pp.128-134
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• 2023

Centella asiatica (C. asiatica) extracts, including asiaticoside and madecassoside, are used in ointments to treat the wound and atopic dermatitis due to their antibacterial and skin-regenerating effects in Asia. Therefore, research on the cultivation and extraction efficiency of C. asiatica is being actively conducted to increase commercialization efficiency. In this study, various deep eutectic solvents (DESs) were prepared and used as the extraction solvents according to the mole ratio between the hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD). And then, the extraction yields in distilled water (DW) and methanol (MeOH), commonly used extraction solvents for C. asiatica, were compared and analyzed by HPLC in the optimized operating condition. As a result, a mixture of DW and DES at a ratio of 3:7 showed about 1.4 times higher extraction efficiency than MeOH only. Conversely, the extraction efficiency in a mixture of MeOH and DES at a ratio of 3:7 was about 6 % lower than that in MeOH only.

• Smart Structures and Systems
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• v.19 no.2
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• pp.115-126
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• 2017

In this paper, size dependent bending and free flexural vibration behaviors of functionally graded (FG) nanobeams are investigated using a nonlocal quasi-3D theory in which both shear deformation and thickness stretching effects are introduced. The nonlocal elastic behavior is described by the differential constitutive model of Eringen, which enables the present model to become effective in the analysis and design of nanostructures. The present theory incorporates the length scale parameter (nonlocal parameter) which can capture the small scale effect, and furthermore accounts for both shear deformation and thickness stretching effects by virtue of a hyperbolic variation of all displacements through the thickness without using shear correction factor. The material properties of FG nanobeams are assumed to vary through the thickness according to a power law. The neutral surface position for such FG nanobeams is determined and the present theory based on exact neutral surface position is employed here. The governing equations are derived using the principal of minimum total potential energy. The effects of nonlocal parameter, aspect ratio and various material compositions on the static and dynamic responses of the FG nanobeam are discussed in detail. A detailed numerical study is carried out to examine the effect of material gradient index, the nonlocal parameter, the beam aspect ratio on the global response of the FG nanobeam. These findings are important in mechanical design considerations of devices that use carbon nanotubes.

• Korean Journal of Clinical Pharmacy
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• v.22 no.2
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• pp.113-122
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• 2012

ACC/AHA/SCAI Guideline recommends for administration dual antiplatelet therapy after drug-eluting stent (DES) to prevent restenosis and stent thrombosis in patients with percutaneous coronary intervention (PCI). Recently triple antiplatelet therapy including cilostazol is known to reduce restenosis and stent thrombosis significantly after DES implantation. However, there is lack of data providing the efficacy of triple antiplatelet therapy. The purpose of this study is to evaluate the clinical effects of the triple therapy after DES implantation compared with the dual therapy. This retrospective study collected data from medical charts of 251 patients who received DES implantation between Jul 2006 and Jun 2008. They received either dual antiplatelet therapy (N = 154 clopidogrel and aspirin; Dual group) or triple antiplatelet therapy (N = 97 cliostazol, clopidogrel and aspirin; Triple group). Major adverse cardiac event rates (MACE, included total death, myocardial infarction, target lesion revascularization) at 12 months, 24 months, stent thrombosis, rates of bleeding complications and adverse drug reactions were compared between these two groups. Compared with the dual group, the triple group had a similar incidence of the MACE rates at 24months (12.3% vs. 12.4%, p = 0.99). There is no difference in overall stent thrombosis between two groups (Dual group 2.6% vs. Triple group 4.1%, p = 0.5). Subgroup analysis showed that diabetic patients got more benefit in reducing MACE rates but, there is no statistical difference. Bleeding complications and adverse drug effects were not different significantly. As compared with dual antiplatelet therapy, triple antiplatelet therapy did not reduce the 12-months, 24-months MACE rates and stent thrombosis. Bleeding complications and adverse drug effects were not different.

• Korean Chemical Engineering Research
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• v.52 no.4
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• pp.481-485
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• 2014

The applicability of the dipping, ultrasonic-assisted, heating methods to the extraction of useful components from Zostera marina was investigated. For the increase of the extraction yield of caffeic acid and rosmarinic acid from Zostera marina, ionic liquids and DES were used as additives in the extraction solvent. The optimum extraction conditions were found: dipping extraction, methanol as extraction solvent, 0.005 g of freeze-dried material powder, 5 mL methanol, 30 min and 0.5 g of DES-3 ($Et_4NCl$ and phenol) as additive. As a result, 0.19 mg/g of caffeic acid and 8.48 mg/g of rosmarinic acid were obtained. This method is simple and sensitive, and has been applied successfully to determine the component of caffeic acid and rosmarinic acid in Zostera marina and these results indicate that DES were used as additives is more suitable than traditional extraction for the extraction of useful components from Zostera marina.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.11
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• pp.883-891
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• 2018

In this paper, aerodynamic noise simulation was conducted using DES (Detached Eddy Simulation) and FW-H (Ffowcs Williams and Hawkings) acoustic analogy for the tandem cylinders which have configuration similar to a landing gear of airplanes. Numerical simulation for the tandem cylinders whose centers are 3.7D apart was carried out and results were compared with the measured data such as flow characteristics, pressure coefficients on the cylinder surfaces and far-field noise characteristics. It was confirmed that periodically shedded vortices released at the upstream cylinder and impinged on the downstream cylinder surface are major sources of aerodynamic noise. After verifying the computational method of using DES and FW-H acoustic analogy for predicting aerodynamic noise of tandem cylinders, additional simulation was conducted to examine the effect of attaching a splitter plate at the rear of the upstream cylinder. It was confirmed that the noise level in specific frequency band decreased significantly because the splitter plate changed the vortex shedding features and reduced dipole noise source.

• Journal of Korean Philosophical Society
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• no.88
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• pp.307-335
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• 2010

Gemäß der lernerorientierten Didaktik und Methodik des deutschen neuen Schulfaches "Praktische Philosophie" studiert die vorliegende Forschung die Unterrichtsmethoden zum fruchtbaren Philosophie-, Essay- und Ethikunterricht. Sie stellt die Unterrichtsmethoden unter der Gliederung von 1) Methoden für die Einführung in den Unterricht, 2) die für den Hauptteil des Unterrichts, 3) die für den Abschluss des Unterrichts, 4) die für Strukturierung des ganzen Unterrichts dar. Der zweite Teil wird wiederum in die Methoden der Form vom Sprechen und Horen, vom Lesen und vom Schreiben unterteilt. Wegen der Einschränkung vom erlaubten Umfang für den Aufsatz wird diese Forschung in zwei Aufsätze geteilt. Der erste Aufsatz behandelte den Teil 1) und den ersten Unterteil des Teils 2). Als Methoden für die Einführung in den Unterricht stellten sich Partnerinterview, Brainstorming und Schneeball dar und als Methoden der Form vom Sprechen und Horen Fishbowl, Pro-Kontra-Argumention, Gruppenarbeit und das sokratische Gespräch. Dieser zweite Aufsatz stellt Lesekompetenz, Lesestrategie, Textanalyse, Mindmapping, Dilemma, kooperatives Lesen und Placemat im Bezug auf Methoden der Form vom Lesen dar und das einfache und dialektische Argumentieren, das kreative Schreiben und Gedankenexperiment als Methoden der Form vom Schreiben. Als Methoden für den Abschluss des Unterrichts werden Blitzlicht, Feedback und Postersession und als Methoden für Strukturierung des ganzen Unterrichts Sandwich und Lernstop betrachtet.

• Journal of the Korean Chemical Society
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• v.57 no.5
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• pp.568-573
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• 2013

A selective and sensitive method for simultaneous determination of copper in blood by adsorptive differential pulse cathodic stripping voltammetry is presented. The procedure involves an adsorptive accumulation of Cu(II)-ETSC (4- ethyl-3-thiosemicarbazide) on a hanging mercury drop electrode, followed by a stripping voltammetry measurement of reduction current of adsorbed complex at about -715 mV. The optimum conditions for the analysis of copper (II) ion are : pH 10.3, concentration of 4-ethyl-3-thiosemicarbazide $3.25{\times}10^{-6}$ M and an accumulation potential of -100 mV. The peak current is proportional to the concentration of copper over the range 0.003-125 ng/mL with a detection limit of 0.001 ng/mL and an accumulation time of 60 s. Moreover, with the use of the proposed method, there is a considerable improvement in the detection limit, the linear dynamic range and the deposition time, compared with the methods of adsorptive stripping voltammetry for the determination of copper. The developed method was validated by analysis of whole blood certified reference materials.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.640-645
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• 2005

The function of the desVIII gene in the pikromycin producer Streptomyces venezuelae was characterized by gene deletion and complementation analysis. In addition to the DesVII glycosyltransferase, the desVIII gene that has previously been suggested to be required for the incorporation of endogenous deoxysugar, TDP-D-desosamine, into the aglycone of pikromycin is also required for the transfer of exogenous deoxysugars, TDP-D-quinovose and TDP-D-olivose.

• Journal of the Optical Society of Korea
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• v.19 no.6
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• pp.592-603
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• 2015

In this paper, an optical implementation of a novel asymmetrical cryptosystem combined with D-H secret key sharing and triple DES is proposed. The proposed optical cryptosystem is realized by performing free-space interconnected optical logic operations such as AND, OR and XOR which are implemented in Mach-Zehnder type interferometer architecture. The advantage of the proposed optical architecture provides dual outputs simultaneously, and the encryption optical setup can be used as decryption optical setup only by changing the inputs of SLMs. The proposed cryptosystem can provide higher security strength than the conventional electronic algorithm, because the proposed method uses 2-D array data, which can increase the key length surprisingly and uses 3DES algorithm, which protects against “meet in the middle” attacks. Another advantage of the proposed asymmetrical cryptosystem is that it is free to change the user’s two private random numbers in generating the public keys at any time. Numerical simulation and performance analysis verify that the proposed asymmetric cryptosystem is effective and robust against attacks for the asymmetrical cipher system.