• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.88-94
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• 2008

Two sugar biosynthetic cassette plasm ids were used to direct the biosynthesis of a deoxyaminosugar. The pOTBP1 plasmid containing TDP-glucose synthase (desIII), TDP-glucose-4,6-dehydratase (desIV), and glycosyltransferase (desVII/desVIII) was constructed and transformed into S. venezuelae YJ003, a strain in which the entire gene cluster of desosamine biosynthesis is deleted. The expression plasmid pOTBP3 containing 4-aminotransferase (gerB) and 3,5-epimerase (orf9) was transformed again into S. venezuelae YJ003-OTBP1 to obtain S. venezuelae YJ003-OTBP3 for the production of 4-amino-4,6-dideoxy-L-glucose derivatives. The crude extracts obtained from S. venezuelae ATCC 15439, S. venezuelae YJ003, and S. venezuelae YJ003-OTBP3 were further analyzed by TLC, bioassay, HPLC, ESI/MS, LC/MS, and MS/MS. The results of our study clearly shows that S. venezuelae YJ003-OTBP3 constructs other new hybrid macrolide derivatives including 4-amino-4,6-dideoxy-L-glycosylated YC-17 (3, [M+ $Na^+$] m/z=464.5), methymycin (4, m/z=480.5), novamethymycin (6, m/z=496.5), and pikromycin (5, m/z=536.5) from a 12-membered ring aglycon (10-deoxymethynolide, 1) and a 14-membered ring aglycon (narbonolide, 2). These results suggest a successful engineering of a deoxysugar pathway to generate novel hybrid macrolide derivatives, including deoxyaminosugar.

• 분석과학
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• 제36권3호
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• pp.128-134
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• 2023

병풀(Centella asiatica) 추출물인 asiaticoside와 madecassoside는 항균성 및 피부재생 등의 효과로 인해 아시아 지역에서 치료용 연고에 사용되고 있으며, 병풀의 재배 및 추출효율을 높이기 위한 연구가 상업화를 위해 활발히 진행되고 있다. 본 연구에서는 환경 친화적인 추출 용매인 deep eutectic solvents(DESs)가 병풀에서 asiaticoside와 madecassoside의 추출 효율에 미치는 영향을 관찰하기 위하여 다양한 수소결합 받개(hydrogen bond acceptor, HBA)와 주개(hydrogen bond donor, HBD)의 혼합 몰비(HBA1: HBD2, HBA1:HBD3, HBA1:HBD4, 그리고 HBA1:HBD5)로 제조하여 추출용매로 사용하였며, 용매 추출에서 일반적으로 사용되고 있는 추출용매인 증류수(DW)와 methanol (MeOH)의 추출 결과를 최적화된 HPLC조건으로 분리하여 정량 분석하였다. 그 결과, DW와 DES를 3:7로 혼합하여 사용한 경우가 단일 MeOH를 사용한 경우보다 약 1.4배 높은 추출 효율을 보였다. 반대로 MeOH과 DES를 3:7로 혼합하여 사용하는 경우에서는 단일 MeOH를 사용하는 경우보다 약 6 % 낮은 추출 효율을 보였다.

• Smart Structures and Systems
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• 제19권2호
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• pp.115-126
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• 2017

In this paper, size dependent bending and free flexural vibration behaviors of functionally graded (FG) nanobeams are investigated using a nonlocal quasi-3D theory in which both shear deformation and thickness stretching effects are introduced. The nonlocal elastic behavior is described by the differential constitutive model of Eringen, which enables the present model to become effective in the analysis and design of nanostructures. The present theory incorporates the length scale parameter (nonlocal parameter) which can capture the small scale effect, and furthermore accounts for both shear deformation and thickness stretching effects by virtue of a hyperbolic variation of all displacements through the thickness without using shear correction factor. The material properties of FG nanobeams are assumed to vary through the thickness according to a power law. The neutral surface position for such FG nanobeams is determined and the present theory based on exact neutral surface position is employed here. The governing equations are derived using the principal of minimum total potential energy. The effects of nonlocal parameter, aspect ratio and various material compositions on the static and dynamic responses of the FG nanobeam are discussed in detail. A detailed numerical study is carried out to examine the effect of material gradient index, the nonlocal parameter, the beam aspect ratio on the global response of the FG nanobeam. These findings are important in mechanical design considerations of devices that use carbon nanotubes.

• 한국임상약학회지
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• 제22권2호
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• pp.113-122
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• 2012

ACC/AHA/SCAI Guideline recommends for administration dual antiplatelet therapy after drug-eluting stent (DES) to prevent restenosis and stent thrombosis in patients with percutaneous coronary intervention (PCI). Recently triple antiplatelet therapy including cilostazol is known to reduce restenosis and stent thrombosis significantly after DES implantation. However, there is lack of data providing the efficacy of triple antiplatelet therapy. The purpose of this study is to evaluate the clinical effects of the triple therapy after DES implantation compared with the dual therapy. This retrospective study collected data from medical charts of 251 patients who received DES implantation between Jul 2006 and Jun 2008. They received either dual antiplatelet therapy (N = 154 clopidogrel and aspirin; Dual group) or triple antiplatelet therapy (N = 97 cliostazol, clopidogrel and aspirin; Triple group). Major adverse cardiac event rates (MACE, included total death, myocardial infarction, target lesion revascularization) at 12 months, 24 months, stent thrombosis, rates of bleeding complications and adverse drug reactions were compared between these two groups. Compared with the dual group, the triple group had a similar incidence of the MACE rates at 24months (12.3% vs. 12.4%, p = 0.99). There is no difference in overall stent thrombosis between two groups (Dual group 2.6% vs. Triple group 4.1%, p = 0.5). Subgroup analysis showed that diabetic patients got more benefit in reducing MACE rates but, there is no statistical difference. Bleeding complications and adverse drug effects were not different significantly. As compared with dual antiplatelet therapy, triple antiplatelet therapy did not reduce the 12-months, 24-months MACE rates and stent thrombosis. Bleeding complications and adverse drug effects were not different.

• Korean Chemical Engineering Research
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• 제52권4호
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• pp.481-485
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• 2014

잘피의 유용성분을 추출하기 위한 dipping, ultrasonic-assisted, heating추출법의 적용성을 검토하였다. 이온성 액체와 공융용매(DES)를 잘피에 존재하는 카페인산과 로즈마린산의 추출 효율을 증가시키기 위해 첨가제로써 사용하였다. 실험에서 확인된 최적의 조건은 동결건조된 0.005 g의 잘피 뿌리 파우더와 5 mL의 methanol 그리고 0.5 g의 DES-3를 바이알에 넣은 후 30분동안 dipping하여 추출하였다. 그 결과 0.19 mg/g 카페인산과 8.48 mg/g의 로즈마린산을 얻었다. 이 방법은 잘피에 있는 카페인산과 로즈마린산을 간단하고 성공적으로 적용할 수 있었고 전통적인 용매 추출법보다 청정용매인 DES를 첨가했을 때 잘피의 유용성분 추출에 더 적합함을 의미한다.

• 한국항공우주학회지
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• 제46권11호
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• pp.883-891
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• 2018

본 연구에서는 항공기 랜딩 기어와 유사한 형태의 탠덤 실린더에 대해 DES (Detached Eddy Simulation)와 FW-H (Ffowcs Williams and Hawkings) 음향상사법을 이용한 전산공력 소음해석을 수행하였다. 실린더 중심 간 거리 3.7D 조건에 대한 전산공력소음해석 결과를 탠덤 실린더를 지나는 외부 유동 특성 및 원거리 소음을 계측한 선행 연구 결과와 비교하였다. 전방 실린더의 상/하부에서 발생한 와류(Vortex)가 특징적인 주파수를 갖고 후방 실린더에 충돌하면서 소음 발생원이 되는 것을 확인하였다. DES 와 FW-H 음향상사법을 이용한 전산공력소음해석 방법의 타당성을 확인한 후, 전방 실린더의 후방에 분할판을 적용한 경우의 전산공력소음해석을 수행하였다. 전방 실린더의 후방에 분할판이 적용되면 전방 실린더의 상/하부에서 발생하는 와류의 특징을 변화시키고 이극자 소음원 크기를 감소시켜 특정 주파수 대역에서 발생하는 소음 크기가 감소하는 것을 확인하였다.

• 철학연구
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• 제88호
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• pp.307-335
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• 2010

본 연구는 독일의 신 교과 실천철학의 학습자 중심의 수업이라는 교수법과 방법론에 따라 효과적인 철학, 논술, 윤리 수업의 모델을 개발하기 위한 방법론 연구이다. 본 연구는 수업의 방법론을 1) 수업의 도입을 위한 방법들, 2) 수업의 본격적인 진행을 위한 방법들, 3) 수업의 중간 점검이나 마무리를 위한 방법들, 4) 수업 전체를 구성하는 방법들로 구분하여 서술한다. 2)는 다시 말하기 듣기 형식의 방법들, 읽기 형식의 방법들, 글쓰기 형식의 방법들로 세분된다. 지면상 본 연구는 두 논문으로 나뉘어 선행 논문에서는 1)과 2)의 첫 부분이 다루어졌다. 수업의 도입을 위한 방법으로는 파트너인터뷰, 브레인스토밍, 눈뭉치기의 방법이 서술되고, 말하기 듣기 형식의 방법으로는 어항 토론, 찬반 논쟁, 조별 작업 및 토론, 소크라테스식 대화의 방법들이 서술되었다. 현 논문에서는 읽기 형식의 방법과 관련하여 읽기 능력, 읽기 전략, 텍스트 분석, 생각지도 그리기, 딜레마 분석, 조별 읽기, 멍석 위에 결과 모으기가, 글쓰기 형식의 방법으로서 단순 논증 형식의 글쓰기, 변증법적 논증 형식의 글쓰기, 창의적 글쓰기, 사유실험이, 수업의 중간 점검이나 마무리를 위해서 카메라 플래시, 피드백, 포스터 전시와 같은 방법들이, 수업 전체를 구성하는 방법으로서 샌드위치, 강의 위주의 수업에서 중간 휴지와 같은 방법들이 고찰된다.

• 대한화학회지
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• 제57권5호
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• pp.568-573
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• 2013

A selective and sensitive method for simultaneous determination of copper in blood by adsorptive differential pulse cathodic stripping voltammetry is presented. The procedure involves an adsorptive accumulation of Cu(II)-ETSC (4- ethyl-3-thiosemicarbazide) on a hanging mercury drop electrode, followed by a stripping voltammetry measurement of reduction current of adsorbed complex at about -715 mV. The optimum conditions for the analysis of copper (II) ion are : pH 10.3, concentration of 4-ethyl-3-thiosemicarbazide $3.25{\times}10^{-6}$ M and an accumulation potential of -100 mV. The peak current is proportional to the concentration of copper over the range 0.003-125 ng/mL with a detection limit of 0.001 ng/mL and an accumulation time of 60 s. Moreover, with the use of the proposed method, there is a considerable improvement in the detection limit, the linear dynamic range and the deposition time, compared with the methods of adsorptive stripping voltammetry for the determination of copper. The developed method was validated by analysis of whole blood certified reference materials.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.640-645
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• 2005

The function of the desVIII gene in the pikromycin producer Streptomyces venezuelae was characterized by gene deletion and complementation analysis. In addition to the DesVII glycosyltransferase, the desVIII gene that has previously been suggested to be required for the incorporation of endogenous deoxysugar, TDP-D-desosamine, into the aglycone of pikromycin is also required for the transfer of exogenous deoxysugars, TDP-D-quinovose and TDP-D-olivose.

• Journal of the Optical Society of Korea
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• 제19권6호
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• pp.592-603
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• 2015

In this paper, an optical implementation of a novel asymmetrical cryptosystem combined with D-H secret key sharing and triple DES is proposed. The proposed optical cryptosystem is realized by performing free-space interconnected optical logic operations such as AND, OR and XOR which are implemented in Mach-Zehnder type interferometer architecture. The advantage of the proposed optical architecture provides dual outputs simultaneously, and the encryption optical setup can be used as decryption optical setup only by changing the inputs of SLMs. The proposed cryptosystem can provide higher security strength than the conventional electronic algorithm, because the proposed method uses 2-D array data, which can increase the key length surprisingly and uses 3DES algorithm, which protects against “meet in the middle” attacks. Another advantage of the proposed asymmetrical cryptosystem is that it is free to change the user’s two private random numbers in generating the public keys at any time. Numerical simulation and performance analysis verify that the proposed asymmetric cryptosystem is effective and robust against attacks for the asymmetrical cipher system.