• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.25 no.3
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• pp.88-96
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• 2012

Objectives : We investigated the skin properties on the periorbital region and cheek of Korean female according to age pattern by 7year based on Sang Go Cheun Jin Ron (上古天眞論) in So Moon (素問,) Methods : 49 Korean females, aged 21-56 years ($39.54{\pm}10.04$ years) were participated in this study. We measured the skin properties of periorbital region and cheek with SkinTouch (Amorepacific, Korea). Skin moisture, sebum, elasticity, lightness, roughness, area of wrinkle, depth of wrinkle were assessed. Results : Differences were noticed depending on the site and age. The level of moisture was higher on the eyelid than crow's feet. The level of lightness and elasticity were lower on the periorbital region than cheek. Skin lightness was highest on group1(under 28y) and lowest on group5(over 49y). Skin roughness was highest on group1(under 28y) and lowest on group4(over 42y under 49y). The level of wrinkle are was lowest on group1(under 28y) and highest on group 5(over49y). The level of wrinkle depth was lowest on group1(under 28y) and highest on group 4(over 42y under 49y). Conclusion : Unique aging pattern by 7 years of female is reflected to diverse skin properties of eyes. It can be possible to apply the theory on Sang Go Cheun Jin Ron (上古天眞論) to practical use in dermatology and cosmetic.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.4 s.34
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• pp.111-121
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• 1999

Acne vulgaris, the most common skin disease. can be formed as only a few comedons or severe inflammatory lesions. The pathogenesis of acne involves various factors; excessive androgen, excessive sebum production, abnormal alteration of follicular epithelium, proliferation of Propionibacterium acnes, and inflammation. We investigated acne therapy using oriental herbs described in the Korean traditional medical book(Dong-ui-bo-gam). Oriental herbs(Angelica daurica, Arctium lappa, Coptidis rhizoma, and Glycyrrhiza glabra) were chosen based on their respective property of sebum control, anti-inflammatory activity, and anti-bacterial activity. We examined the effect of acne treatment, in terms of chemotactic inhibition, lipogenesis inhibition, and anti-bacterial activity for P. acnes. 1. Neutrophil chemotaxis assay; P. acnes secrete chemotactic factors and other pro-inflammatory extracellular products. Neutrophil chemotactic activity of P. acnes was measured by 48-well chemotaxis method. Angelica daurica clearly suppressed chemotactic activity of P. acnes. 2. Using sebaceous gland of hamster ear lipogenesis assay; Sebaceous lipogenesis was measured using ear biopsies by incubation or $C^{14}$-acetate in culture media. The $C^{14}$-labeled lipids were extracted and determined by liquid scintilation counting. Coptidis rhizoma markedly inhibited sebum production. 3. Anti-bacterial assay for P. acnes(MIC test); Glycyrrhiza glabra showed anti-bacterial activity. P. acnes did not develop resistance against Glycyrrhiza glabra. Retinoids are effectively to inhibit sebum production and regulate follicular keratinization process, with little anti-inflammatory activity. Angelica daurica suppressed neutrophil chemotaxis, Coptidis rhizoma inhibited sebum production, and Glycyrrhiza glabra showed anti-bacterial activity against P. acnes. A combined formulation of Angelica daurica, Coptidis rhizoma. and Glycyrrhiza glabra is expected to provide effective acne treatment.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.4
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• pp.321-330
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• 2014

Ultraviolet B (UVB) is a primary environmental factor that induces adverse effects on skin such as photoaging, skin burn and cancer. UVB also increases the expression of $11{\beta}$-hydroxysteroid dehydrogenase type 1 ($11{\beta}-HSD1$), which converts inactive cortisone to active cortisol in response to a variety of stressors in target tissues. Thus, we have screened new herbal extracts that suppress $11{\beta}-HSD1$ expression induced by UVB in human dermal fibroblasts. We also investigated whether Trapa japonica (TJ) extract and its fractions inhibit UVB-induced photoaging in Hs68 cells and 3D skin model. Results showed that TJ extract inhibited the increase of $11{\beta}-HSD1$ expression in UVB-exposed Hs68 cells significantly. TJ extract and its fractions not only inhibited $11{\beta}-HSD1$ expression, but also suppressed the increase of matrix metalloproteinases (MMP-1, 3, 9) and proinflammatory cytokines (IL-6, 8) in UVB-irritated Hs68 cells. TJ extract also inhibited MMP-1 expression in UVB-irritated 3D skin model. In addition, TJ extract recovered UVB-induced decreases of epidermal thickness and PCNA-positive cells in 3D skin model. Taken together, these results suggest that TJ extract and its fractions inhibit UVB-induced skin photoaging by interfering with $11{\beta}-HSD1$ and MMPs.

• Toxicological Research
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• v.34 no.4
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• pp.355-361
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• 2018

Methylisothiazolinone (MIT) has been used in combination with methylchloroisothiazolinone (CMIT) for cosmetic products such as shampoo, body lotion, and skin care products. The mixture of CMIT/MIT has been found to cause allergic contact dermatitis and is thus no longer permitted for use as a preservative in leave-on cosmetics. However, MIT itself was approved as a stand-alone preservative at a maximum concentration of 100 ppm as the toxicity was derived from CMIT rather than MIT. However, in many countries, allergic skin irritation caused by MIT remains a social concern. In this study, skin irritation was assessed for the presence of MIT, propylene glycol, and their mixture using a 3D human skin model $EpiDerm^{TM}$. Although non-diluted MIT causes serious skin toxicity, skin irritation was not observed at a concentration of 100 ppm, the maximum permissible level for cosmetics and personal care products according to European regulations. Propylene glycol, the most widely used vehicle for MIT, did not cause skin irritation in the 3D skin model. The results are expected to provide information for regulatory policies and guidelines on the use of biocides in consumer products.

• Medical Lasers
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• v.9 no.2
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• pp.142-149
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• 2020

Background and Objectives A picosecond-domain laser treatment using a microlens array (MLA) or a diffractive optical element elicits therapeutic micro-injury zones in the skin. This study examined the patterns of tissue reactions after delivering multiple pulses of 1,064-nm, MLA-type, picosecond neodymium:yttrium-aluminum-garnet laser treatment. Materials and Methods Multiple pulses of picosecond laser treatment were delivered to ex vivo human or brown micropig skin and analyzed histopathologically. A high-speed cinematographic study was performed to visualize the multiple pulses of picosecond laser energy-induced skin reactions in in vivo human skin. Results In the ex vivo human skin, a picosecond laser treatment at a fluence of 0.3 J/cm² over 100 non-stacking passes generated multiple lesions of thermally-initiated laser-induced optical breakdown (TI-LIOB) in the epidermis and dermis. In the ex vivo micropig skin, stacking pulses of 20, 40, 60, 80, and 100 at a fluence of 0.3 J/cm² generated distinct round to oval zones of tissue coagulation in the mid to lower dermis. High-speed cinematography captured various patterns of twinkling, micro-spot reactions on the skin surface over 100 stacked pulses of a picosecond laser treatment. Conclusion Multiple pulses of 1,064-nm, MLA-type, picosecond laser treatment elicit marked TI-LIOB reactions in the epidermis and areas of round to oval thermal coagulation in the mid to deep dermis.

• Toxicological Research
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• v.17 no.3
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• pp.167-171
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• 2001

Oral mucous membrane test using Syrian hamsters was performed to evaluate the reliability as a model system for the assessment of the potentially irritating substances intended for the mucous membranes, and to determine the irritating potential of a new emulsion-type formulated toothpaste. After test substances were implanted into the cheek pouches of hamsters with diluents (20 mg/kg) under pento-barbital sodium anesthesia, we made the comparison in irritation between emulsion-type and dispersion-type of triclosan (TCS) formulations in the range of 0.2% to 0.3%. The emulsion-type formulations using non-ionic surfactant showed less mucosal lesion than other commercial toothpastes with 0.3% TCS, or dispersion-type ones. However, no significant difference in irritation was detected between 0.2% and 0.3% TCS. We report that this hamster cheek pouch method could be a reliable approach for the evaluation slight difference in the irritating potentials of cosmetics and hygiene products intended for the lips or other mucous membranes, and this method showed that the new emulsion-type formulation significantly lowered the TCS-induced toxicity, compared with other commercial toothpastes.

• Journal of Internet Computing and Services
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• v.10 no.4
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• pp.25-33
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• 2009

In the paper, we proposed a new system to retrieve 3D wrinkle data based on stereo images. Usually, 3D reconstruction based on stereo images or video is very popular and it is the research focus, which has been applied for culture heritage, building and other scene. The target is object measurement, the scene depth calculation and 3D data obtained. There are several challenges in our research. First, it is hard to take the full information wrinkle images by cameras because of light influence, skin with non-rigid object and camera performance. We design a particular computer vision system to take winkle images with a long length camera lens. Second, it is difficult to get the dense stereo data because of the hard skin texture image segmentation and corner detection. We focus on semi-dense stereo matching algorithm for the wrinkle depth. Compared with the 3D scanner, our system is much cheaper and compared with the physical modeling based method, our system is more flexible with high performance.

• The Korea Journal of Herbology
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• v.22 no.2
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• pp.45-50
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• 2007

objectives: We investigated the moisturizing and cooling effects of the cosmetic product with Scrophularia Buergeriana, Poria Cocos, Lonicera Japonica, Portulaca Oleracea and Ginko Biloba extract on human skin by using non-invasive instrument. Methods: We measured physiological effects after volunteers(female, n=5) applied the cosmetic product containing the extract of oriental herbs. The forearm skin moisturization was measured with $Corneometer^{\circledR}$ CM825 after 15min, 3hr, and 6hr. The forearm skin surface roughness was with Scanning electron microscopy after 15min, and 6hr. The face skin temperatures cooling and microcirculation effects were measured with ThermaCAMTM PM525 and LDl-Periscan PlM II after 15min, and 30min. Results: The forearm skin moisturization was imcreased 108% after 15min. The forearm skin surface roughness was decreased significantly after 15min. The face skin temperatures were cooling down to $30.7^{\circ}C$. The face skin microcirculation effect was normalized time-dependently. Conclusions: We concluded that cosmetic product containing the extract of Scrophularia Buergeriana, Poria Cocos, Lonicera Japonica, Portulaca Oleracea and Ginko Biloba had a moisturization effect and improving the surface roughness, temperature and microcirculation on the human skin.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.741-762
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• 2003

Ginsenosides, the major active ingredients of ginseng, show a variety of biomedical efficacies such as anti-aging, anti oxidation and anti-inflammatory activities. To understand the effects of compound K (20-O-D-glucopyranosyl-20 (S)-protopanaxadiol), one of the major metabolite of ginsenosides on the skin, we assessed the expression level of ∼ 100 transcripts in compound K-treated HaCaT cells using cDNA microarray analysis. Compound K treatment induced differential expression of 21 genes, which have been reported to be involved in the organization of ECM structure as well as defense responses in human skin cells. One of the most interesting findings is 2-fold increase in hyaluronan synthase2 (HAS2) gene expression by compound K. We found that change in expression of HAS2 gene represents a specific response of HaCaT cells to compound K because hyaluronan synthase 1, 3 was not changed by treatment with compound K. We also demonstrated that the compound K effectively induced hyaluronan synthesis in human skin cells and hairless mouse skin. The human clinical study indicates that topical application of compound K-containing oil-in-water emulsion showed improvement of xerosis, wrinkle and fine lines in the aged skin. We concluded that compound K has anti-aging effects by the induction of HAS2 gene expression and following hyaluronan synthase.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.1
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• pp.47-58
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• 2023

Recently, along with the remarkable increase in interest in microbiome cosmetics, the application of microbiome extracts in the complex efficacy as anti-aging, brightening etc. has become very important. In this study, Bifidobacterium bifidum (B. bifidum), which has excellent anti-wrinkle efficacy among the microbiome, was selected through an in vitro test using cells and 3D human skin equivalents. And the anti-wrinkle efficacy of cosmetics containing B. bifidum was evaluated by clinical test. Thereafter, the cytotoxicity, anti-oxidation, anti-inflammatory and anti-wrinkle efficacy of the of the bifida fermented filtrate were tested. An emulsion containing bifida fermented filtrate at a concentration of 5% (v/v) with no cytotoxicity and excellent efficacy was prepared with the placebo emulsion. The clinical test was conducted on a total of 21 women at 2 weeks comparing the bifida emulsion and placebo emulsion. Wrinkles around the eyes were instrumentally evaluated using Antera 3D. The wrinkle reduction rate of the Bifida emulsion group compared with the placebo emulsion group differed by 5.6%. Overall, the selection of microbiome using 3D human skin equivalents and the efficacy study of cosmetics with the microbiome extracts would be actively studied to the development of microbiome cosmetics and skin microbiome mechanisms.