• Korean Journal of Animal Reproduction
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• v.18 no.1
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• pp.39-46
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• 1994

This experiment was carried out to develop an in vitro culture system for rabbit embryos. The zygotes or 2-cell embryos were collected from the oviducts of the superovulated and mated does with D-PBS/10% FCS at 24 hours after hCG injection. The in vitro developmental rate of blastocyst formation and the number of nuclei in the embryos were examined under the following treatments; 1) TCM-199 with 10% FCS, 2) EBSS with 10% FCS, 3) rabbit vitreous humor(VH), 4) TCM-199 with 10% FCS＋BOEC, 5) TCM-199 with 10% FCS＋ROEC, 6) EBSS with 10% FCS＋BOEC and 7) EBSS with 10% FCS＋ROEC. For a comparative study of in vivo and in vitro development, the fresh blastocysts, which were developed in vivo for 96 hours after hCG injection, were collected from the uterus and their numbers of nuclei were counted. 1. The zygotes or 2-cell embryos developed to the blastocyst stage in TCM-199, EBSS and VH at the rates of 93, 92 and 89%, respectively. 2. The higher developmental rates 95~98% of blastocyst formation was achieved when the embryos were co-cultured with a monolayer of bovine or rabbit oviductal epithelial cells in TCM-199 or EBSS. No significant difference in developmental rates was shown between bovine and rabbit oviductal epithelial cells. 3. In a comparative study of in vivo and in vitro development, the total numbers of nuclei were significantly less in the in vitro cultured embryos(104~224) than the in vivo developed embryos(1, 0090 at 96 hours after hCG injectin. 4. The mean cell cycle numbers in the embryos cultured for 72 hours in TCM-199 with 10% FCS, EBSS with 10% FCS, TCM-199 with 10% FCS＋BOEC, TCM-199 with 10% FCS＋ROEC, EBSS with 10% FCS＋BOEC and in vivo was 7.38, 6.63, 7.76, 7.69, 7.01 and 9.92, respectively. From these results, it can be suggested the optimal culture system for in vitro culture of rabbit embryos is a co-culture system with bovine or rabbit oviductal epithelial cells in TCM-199 with 10% FCS. Considering the significant reduction in total numbers of nuclei in the in vitro cultured embryos, the advanced research on development of in vitro culture system for rabbit embryos is expected.

• Cartoon and Animation Studies
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• s.45
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• pp.27-50
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• 2016

The present study argues that documentary-animation films, which are based on actual human voices, on the level of representation, constitute a new expansion for the medium of animation films, which serve as testimonies to the real world. Animation films are produced using very diverse techniques so that they are complex to the degree of being indefinable, and documentary films, though based on objective representation, increase in complexity in that there exist various types of artificial interventions such as direction and digital image processing. Having emerged as a hybrid genre of the two media, documentary-animation films draw into themselves actual events and elements so that they conceptually share reality-based narratives and are visually characterized by the trappings of animation films. Generally classified as 'animated documentaries', this genre triggered discussions following the release of , a work that is mistaken as having used rotoscoping transforming live action in terms of the technique. When analyzed in detail, however, this work is presented as an ambiguous medium where the characteristics of animation films, which are virtual simulacra without reality, and of documentaries, which are based on the objective indexicality of the referents, coexist because of its mixed use of typical animation techniques, 3D programs, and live-action images. Discussed in the present study, , , and share the characteristics of the medium of documentaries in that the narratives develop as testimonies of historical figures but, at the same time, are connected to animation films because of their production techniques and direction characteristics. Consequently, this medium must be discussed as a new expansion rather than being included in the existing classification system, and such a presupposition is an indispensable process for directly facing the reality of the works and for developing discussions. Through works that directly use the interviewees' voices yet do not transcend the characteristics of animation films, the present study seeks to define documentary-animation films and to discuss the possibility of the medium, which has expanded as a testimony to the real world.

• Korean Journal of Microbiology
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• v.35 no.1
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• pp.7-12
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• 1999

By SDS-polyacrylamide gel elcctrophoresis (SDS-PAGE) and immunoblot analysis that a protein with 66,000 daltons in size was recognized by P. aeruginosa anti-exotoxin A from P. sp. PY002. The yields of exotoxin A in P. sp. PY002 culture were influenced by the concentration of iron in the culture media. Increasing of the exotoxin A production and siderophore production was made slight increasing in the MKB medium. On the other hand, to clone the gene encoding the exoloxin A genomic library of P. sp. PY002 was constructed in pBluescript SK(+). From this library a exotoxin A homologous gene was isolated by immunological hybridization method using P. aemginosa anti-exoloxin A as a probe. Two putative clones were isolated and designated pETA23 and pETA42. Thc restriction analysis ol pETA42 demonstrated that thc 1760 bp insert contained one NcoI, PvuII, SstI, Kpnl and EcoRI site and three SmaI and HaeD sites.

• Korean Journal of Microbiology
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• v.51 no.1
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• pp.86-95
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• 2015

To study the halobacterial diversity at the rhizospheric soil of coastal plant native to Dokdo islands, several host plant were selected and its rhizospheric soil was sampled. Soil sample was diluted serially and pure isolation was done by sub-culture using marine agar media. 26 halophilic strains cultivable at the marine medium containig concentration of 9.0% sodium chloride were selected among total 161 isolates. Their partial 16S rRNA gene sequences extracted from genomic DNA were analyzed and partially identified. Furthermore, to identify their genetic relationship, phylogenetic tree was deduced. Total 26 strains were belongs to Firmicutes (30.8%), Gamma proteobacteria (53.8%), Bacteroidetes (7.7%), Alpha proteobacteria (7.7%), and Actinobacteria (7.7%). These results showed the specific difference from previous researches which has been reported the microbial flora of soil or sea water around the Dokdo islands. Furthermore, 4 among 26 halophilic strains grew at above 12.0% NaCl concentrated marine broth, and 2 strains Idiomarina abyssalis LM4H23 and Halomonas huangheensis AS4H13 grew at 15.0% concentration. These halophilic strains thought to overcoming the severe stress like high salt concentration or variation derived from Dokdo-specific climate and might have unknown, specific relationship with their host coastal plant native to Dokdo islands.

• Journal of Hydrogen and New Energy
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• v.11 no.1
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• pp.43-49
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• 2000

The heat transportation from a complex of industry to a rural area needs more efficient method because the distance between them is usually more than 10km. Conventional heat transportation using steam or hot water via pipe line has limits in transportation distance (about 3~5 km) because of the heat loss and frictional loss in the pipe line. Metal hydride can absorb or discharge hydrogen through exothermic or endothermic reaction. After releasing hydrogen from metal hydride by means of the waste heat from industry, we can transport this hydrogen to urban area via pipe line. In urban areas, other metal alloy reacts with this hydrogen to form metal hydride and produces heat for heating. Cool heat is also obtained if it is possible to use metal hydride with low reaction temperature. Therefore, metal hydride can be used as a media for transportation and storage of heat. $MmNi_{4.5}Al_{0.5}Zr_{0.003}$, $LaNi_5$, $Zr_{0.9}Ti_{0.1}Cr_{0.6}Fe_{1.4}$, $MmNi_{4.7}Al_{0.1}Fe_{0.1}V_{0.1}$ alloys were selected for this purpose and the properties of those metal hydrides were discussed. The design and control techniques were proposed and discussed for this heat transportation system using metal hydride.

• Journal of Adhesion and Interface
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• v.18 no.2
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• pp.75-81
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• 2017

In this study, we synthesized mesoporous carbon (Carbonized Ni-FDU-15) containing nanoporous structures and magnetic nanoparticles. Carbonized Ni-FDU-15 was synthesized via evaporation-induced self-assembly (EISA) and direct carbonization by using a triblock copolymer (F127) as a structure-directing agent, a resol precursor as a carbon-pore wall forming material, and nickel (II) nitrate as a metal ion source. The mesoporous carbon has a well-ordered two-dimensional hexagonal structure. Meanwhile, nickel (Ni) metal and nickel oxide (NiO) were produced in the magnetic nanoparticles in the pore wall. The size of the nanoparticles was about 37 nm. The surface area, pore size and pore volume of Carbonized Ni-FDU-15 were $558m^2g^{-1}$, $22.5{\AA}$ and $0.5cm^3g^{-1}$, respectively. Carbonized Ni-FDU-15 was found to move in the direction of magnetic force when magnetic force was externally applied. The magnetic nanoparticle-bearing mesoporous carbons are expected to have high applicability in a wide variety of applications such as adsorption/separation, magnetic storage media, ferrofluid, magnetic resonance imaging (MRI) and drug targeting, etc.

• Journal of Embryo Transfer
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• v.32 no.3
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• pp.65-71
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• 2017

The elevated temperature and high humidity has been known as main reason for heat stress on animals and cause detrimental effects on productivity of organisms and physiological conditions of normal bioactivities. The aims of this study were to evaluate the relationship between time of heat shock simulation during in vitro maturation and developmental competence of subsequent embryo after in vitro fertilization. Heat shocked cumulus-oocyte complexes (COCs) of Korean native cattle were subjected to normal conditions for 22, 21, 18 and 12 h respectively and transferred to heat stress inducing condition at $40.5^{\circ}C$ in other incubator for 0 (control), 1 and 4 h. After maturation for 22 h, the oocytes were fertilized and cultured in mSOF media for 8 d and examined the developmental capacity of embryos. There were no differences in maturation and cleavage rates between 0, 1 and 4 h heat socked oocytes, but blastocysts formation were lower in the 4 h heat stressed oocytes. The apoptotic cells of developed blastocysts were also increased in at day 8 with 4 h heat shocked oocytes. These results indicate that heat shock on oocytes during maturation could cause negative effects on the developmental competence of embryos.

• Archives of design research
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• v.17 no.3
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• pp.335-342
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• 2004

Recently there have been various research activities regarding Korean traditional aesthetics. However, those researches were mainly conducted individually, partially, and periodically, which resulted in unsystematic and incomprehensive works. Therefore, it is required to orginze all the precedent research works with more systematic and objective framework. Generally speaking, all the human activities including aesthetic activity have ends, procedure and means. In other words, human being needs three key elements for realizing any thought and those three elements include contents, formal, and practical element. Element of contents is ultimate goal to accomplish as value, concept, and meaning of thought with their aims. Formal element includes methods, principles, norms, procedure, formality and style comprising of thought in order to accomplish the goal. Finally, practical element refers to specific means, tool, media, material and techniques to concretize the contents through form. Almost all of thoughts and meaning which human being tries to express consist of language. Major elements in sentence include 'subject (omissible)' , 'objects (aim)', 'predicate (formality)', 'complement (means)' and they are composed systematically and hierarchically with rules in sentence. The study compared human activity model with language structure and analyzed their implication with design (aesthetics), which made it possible to propose analytic frameworks for traditional aesthetics. In addition, the study also systematically organized the way to realize traditional aesthetic value in the present context based on the methods developed in this study.

• Journal of Broadcast Engineering
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• v.24 no.6
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• pp.919-927
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• 2019

In this paper, we propose an accurate camera calibration method for acquiring multiview stereoscopic images. Generally, camera calibration is performed by using checkerboard structured patterns. The checkerboard pattern simplifies feature point extraction process and utilizes previously recognized lattice structure, which results in the accurate estimation of relations between the point on 2-dimensional image and the point on 3-dimensional space. Since estimation accuracy of camera parameters is dependent on feature matching, accurate detection of checkerboard corner is crucial. Therefore, in this paper, we propose the method that performs accurate camera calibration method through accurate detection of checkerboard corners. Proposed method detects checkerboard corner candidates by utilizing 1-dimensional gaussian filters with succeeding corner refinement process to remove outliers from corner candidates and accurately detect checkerboard corners in sub-pixel unit. In order to verify the proposed method, we check reprojection errors and camera location estimation results to confirm camera intrinsic parameters and extrinsic parameters estimation accuracy.

• Journal of the Korean Society of Safety
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• v.38 no.2
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• pp.96-103
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• 2023

Generally, human stampedes and crowd collapses occur when people press against each other, causing falls that may result in death or injury. Particularly, crowd accidents have become increasingly common since the 1990s, with an average of 380 deaths annually. For instance, in Korea, a stampede occurred during the Itaewon Halloween festival on October 29, 2022, when several people crowded onto a narrow, downhill road, which was 45 meters long and between 3.2 and 4 meters wide. Precisely, this stampede was primarily due to the excessive number of people relative to the road size. Essentially, stampedes can occur anywhere and at any time, not just at events, but also in other places where large crowds gather. More specifically, the likelihood of accidents increases when the crowd density exceeds a turbulence threshold of 5-6 /m². Meanwhile, festivals and events, which have become more frequent and are promoted through social media, garner people from near and far to a specific location. Besides, as cities grow, the number of people gathering in one place increases. While stampedes are rare, their impact is significant, and the uncertainty associated with them is high. Currently, there is no scientific system to analyze the risk of stampedes due to crowd concentration. Consequently, to prevent such accidents, it is essential to prepare for crowd disasters that reflect social changes and regional characteristics. Hence, this study proposes using digital topographic maps and crowd-density risk simulations to develop a 3D model of the region. Specifically, the crowd density simulation allows for an analysis of the density of people walking along specific paths, which enables the prediction of danger areas and the risk of crowding. By using the simulation method in this study, it is anticipated that safety measures can be rationally established for specific situations, such as local festivals, and preparations may be made for crowd accidents in downtown areas.