• Title/Summary/Keyword: 3D line

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Screening of Resistant Lines and Yield Losses to F1 Corn from Feeding by the Aphid and Mite (진딧물과 응애 가해에 의한 옥수수 F1의 수량감소율 과 저항성계통 선발)

  • Lee, Joon-Soo;Kim, Soon-Kwon
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.555-564
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    • 2010
  • Aphid and mite are the major insects that harm corn. These insects are difficult to control because they are very sensitive to environmental conditions. The purpose of this experiment was to information the loss in quantity of corn and to select inbred lines with resistance after exposing corn to aphid and mite. When exposed to aphid, the average loss in grain yield, ear length, ear diameter, 100 kernel weight, kernel length, kernel width, and kernel thickness were 18.4, 10.9, 1.7, 5.4, 2.3, 1.5 and -0.2%, respectively. When exposed to mite, the average loss in grain yield, ear length, ear diameter, 100 kernel weight, kernel length, kernel width, and kernel thickness were 49.7, 16.5, 20.7, 18.9, 9.5, and -3.4, respectively. Mite had more significant impact on the loss than aphid. Among these factors, grain yield and ear dimater were most significantly correlated on the loss to aphid ($r=0.83^{**}$), while kernel length and thickness were most significantly correlated on the loss to mite ($r=0.83^{**}$). However, ear length was not related to loss to the two insects. The inbred lines that were often used for $F_1$ hybrid which was lower than the average loss by aphid, were KS7, B68, 61B3, 70A1, and KS5, while the inbred lines that were often used for $F_1$ hybrid which was lower than the average decrease by mite, were KS7, B68, 61B3, 66B2-4, 70A1, 72B2, and KS5. These inbred lines could be used to breed inbred lines, hybrid, and open pollination variety that confer resistant to aphid and mite.

Consecutive automated production of carbon-11 labeled radiopharmaceuticals by sharing 11C-methylation reagent from one 11C-synthetic module

  • Park, Hyun Sik;Lee, Hong Jin;An, Hyun Ho;Moon, Byung Seok;Lee, Byung Chul;Kim, Sang Eun
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.2 no.2
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    • pp.123-131
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    • 2016
  • Increasing clinical demand for carbon-11 labeled radiopharmaceuticals has triggered technological advances in fields of radiochemistry and automated modules. Even though carbon-11 has a short half-life ($t_{1/2}=20.4min$), the consecutive second production of carbon-11 labeled radiopharmaceutical in one $^{11}C$-synthetic module should be delayed at least over 4 h to avoid the high radiation exposure. We herein aimed to produce two different carbon-11 labeled radiopharmaceuticals ([$^{11}C$]PIB and [$^{11}C$]methionine) by sharing of [$^{11}C$]methylation source in one $^{11}C$-synthetic module. The synthesis of $^{11}C$-labeling reagents ($[^{11}C]CH_3I$ or $[^{11}C]CH_3OTf$) is fully automated using the commercial TRACERlab $FX_{C-pro}$ module and is readily adaptable to $^{11}C$-labeling reactor for [$^{11}C$]PIB as well as another $^{11}C$-labeling apparatus for [$^{11}C$]methionine via the three-way valve. After completing the [$^{11}C$]PIB production, the re-synthesized $[^{11}C]CH_3I$ was passed through the three-way valve connected the polyetheretherketone (PEEK) line and loaded into the C18 Sep-Pak cartridge including the methionine precursor. The labeled product [^${11}C$]methionine was purified by a simple cartridge separation and reformulated into saline. The radiochemical yield of [$^{11}C$]PIB and [$^{11}C$]methionine were $5.3{\pm}0.6%$ and $18.7{\pm}0.8%$ (n.d.c.), respectively, with over 97% of radiochemical purity. The specific activity of [$^{11}C$]PIB was over $110GBq/{\mu}mol$. Total production time of two radiopharmaceuticals needs about 2 h from $1^{st}$ beam irradiation including quality control tests. Final [$^{11}C$]PIB and [$^{11}C$]methionine were satisfied all quality control test standards.

The Effects of Cyber Education in RN-BSN's Courses (RN-BSN 과정에서 사이버교육의 효과)

  • Kim, Hee-Soon;Oh, Ka-Sil;Lee, Kyung-Ja;Chang, Hwa-Kyoung
    • The Journal of Korean Academic Society of Nursing Education
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    • v.9 no.2
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    • pp.212-221
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    • 2003
  • The purpose of this study was to evaluate the effects and define the educational strategies of the cyber education offered to students for Bachelor of Science degree program(RN-BSN). The participants in this study were 67 students in two courses, Growth and Development, and Nursing Process. The main components of the cyber classes were the electronic board and on-line discussion board. The study was conducted from March 4, 2001 to July 23, 2001 at Y University in Seoul, Korea. To examine the effects of the cyber education, learning motivation, and learning satisfaction were measured by questionnaires to the students before the initial class and after completing the class. Learning achievement was measured by the grades for each course. The data were analyzed using frequencies, t-test, and paired t-test. The specific results of the course evaluation were compared and analysis was done to examine differences between traditional classroom teaching and cyber teaching. The study results are as follows: 1. There were no significant differences on total scores for learning motivation between pre-cyber education and post-cyber education. However, there were significant decreases in the post test compared to the pre test for the items, ' I'd like to get as high a score as possible compared to other students (t=-2.20, p=.03)' and ' I'm sure to acquire good grades(t=-5.22, p=.00) '. 2. The average score for learning satisfaction using cyber education was high at 3.52. 3. To define learning achievement using cyber education, grades for students taking cyber classes this year were compared to student grades for a class using traditional lassroom teaching last year. The score was significantly higher for classroom students in the 'Growth and Development' course (t=-3.5, p<.001), and the score was significantly higher for the cyber education students in the 'Nursing Process' course (t=4.3, p<.000). 4. The average of post scores on computer competency was significantly higher on six items, general knowledge about computers, data management and data research, ability to communicate using computer, and internet surfing. On the basis of the above findings, this study suggests that cyber education in nursing courses is effective and readily available. However, it is recommended that consideration be given to characteristics of the course when developing cyber education programs for nursing courses.

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Characterization of Prunus necrotic ringspot virus Isolate from Peach in Korea (국내 복숭아에서 분리한 Prunus necrotic ringspot virus의 특성)

  • Kim, Hyun-Ran;Lee, Sin-Ho;Shin, Il-Sheob;Kim, Jeong-Hee;Cho, Kang-Hee;Heo, Seong;Kim, Jeong-Soo;Choi, Yong-Mun
    • Research in Plant Disease
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    • v.15 no.3
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    • pp.170-174
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    • 2009
  • In this paper, we report a characterization of Prunus necrotic ringspot virus (PNRSV) isolate. The virus was identified from 'Yumyeong' peach showing mild mosaic on leaves in commercial orchard of 'Umsung', Chungbuk province in Korea. The virus isolate produced ringspot symptom on the inoculated cotyledons and systemic mosaic and malformation on the upper leaves of Cucumis sativus. Systemic mottles were appeared in Chenopodium quinoa. When the buds of the virus infected stem were grafted on the healthy young Prunus persica GF305 seedlings, line pattern with mosaic appeared within 3 months. Isometric virus-like particles were found in parenchyma cells and plasmodesmata of C. sativus leaves inoculated mechanically with the virus. The cDNA fragments of PNRSV coat protein (CP) region, approximately 675bp, were synthesized from genomic RNA extracted from virus-infected leaves by RT-PCR using specific primer pairs. Partial nucleotide sequences of the CP regions were determined and analyzed with the known PNRSV. The CP gene of PNRSVKorea isolates showed 93.9~94.7% similarity to the 4 known PNRSV isolates.

Inhibitory Effect of Dendrobium moniliforme on Degranulation and Histidine Decarboxylase Expression in RBL-2H3 Cells (RBL-2H3 세포에서 탈과립과 histidine decarboxylase 발현에 미치는 석곡(Dendrobium monilifrme)의 효과)

  • Young Ji Lee;Iskander Madhi;YoungHee Kim
    • Journal of Life Science
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    • v.33 no.2
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    • pp.176-182
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    • 2023
  • The stems of Dendrobium moniliforme are used in traditional Oriental medicine as a Yin tonic to nourish the stomach, promote the production of body fluid, and reduce fever. This study investigated the effects of the aqueous extract of D. moniliforme stems (DME) on mast cell degranulation and the expression of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and histamine-synthesizing enzyme histidine decarboxylase (HDC). We used rat mast cell line RBL-2H3 cells and stimulated them with PMA plus calcium ionophore (PMACI). Pretreatment with DME significantly inhibited PMACI-induced β-hexosaminidase release and the expression of TNF-α, IL-4, and HDC. Furthermore, DME suppressed PMACI-induced nuclear translocation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1). In addition, HDC expression was inhibited by SP600125 (JNK inhibitor), PD98059 (ERK inhibitor), and SB203580 (p38 kinase inhibitor). Finally, the phosphorylation of p38 kinase, extracellular signal-regulated kinase 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) was inhibited by pretreatment with DME. These results suggest that DME has inhibitory effects against degranulation, cytokine (TNF-α and IL-4) and HDC expression, and that HDC expression is mediated by MAPK signaling. These findings suggest that DME may have therapeutic potential in the treatment of hypersensitive and inflammatory diseases.

6G Technology Competitiveness and Network Analysis: Focusing on GaN Integrated Circuit Patent Data (6G의 기술경쟁력 및 네트워크 분석: GaN 집적회로 특허 데이터 중심)

  • Woo-Seok Choi;Jin-Yong Kim;Jung-Hwan Lee;Sang-Hyun Choi
    • Journal of Industrial Convergence
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    • v.21 no.3
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    • pp.1-15
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    • 2023
  • Expectations for wireless communication technology are rising as a base technology that promotes innovation in various industries in line with the paradigm of digital transformation in the 21st century beyond the stage of being used only for communication service itself. In this study, in order to compare 6G technological competitiveness between Korea and leading countries, technological competitiveness was confirmed through PFS, CPP, and network analysis based on GaN Integrated Circuit patent data. Korea's 6G technological competitiveness was 0.62 in PFS and 3.93 in CPP, which were 32.8% and 19.9%, respectively, compared to leading countries. In addition, as a result of network analysis, the collaboration rate in the 6G field was 7.2%, and the collaboration ecosystem was very insufficient in most countries. In contrast, it was confirmed that Korea, unlike leading countries, has established a small-scale collaboration ecosystem linked by industry and academia. Thus, it is necessary to establish a strategy for 6G communication technology at the national level so that communication technology can be advanced based on a relatively well-established collaborative ecosystem.

Effects of Dietary Supplementation of Activated Charcoal Mixed with Wood Vinegar on Broiler Performance and Antibiotics Residue in Eggs (목초액 함유 활성탄의 첨가가 육계 생산성 및 계란 내 항생제 잔류에 미치는 영향)

  • Sung, E.I.;You, S.J.;Ahn, B.K.;Jo, T.S.;Ahn, B.J.;Choi, D.H.;Kang, C.W.
    • Korean Journal of Poultry Science
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    • v.33 no.4
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    • pp.283-293
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    • 2006
  • Two experiments were conducted to investigate the effects of dietary activated charcoal mixed with wood vinegar (AC) on broiler performance and antibiotics residue in eggs. In experiment 1, a total of low hundred fifty, 1-day-old male broile. chicks (Ross) were divided into 6 groups, consisting 3 replicates of 25 birds each, and 134 one of the six experimental diets: devoid of AC and antibiotics (negative control), devoid of AC with 0.1% antibiotics (positive control), devoid of antibiotics with 1% AC, with 0.1% antibiotics and 1% AC, devoid of antibiotics with 2% AC, with 0.1% antibiotics and 2% AC, for 5 wks. Feed consumption and body weights were measured weekly. At the end of the experiment, eight birds from each group were selected and sacrificed. The relative weight of organs and characteristics of breast muscle were investigated. There were no significant differences in feed intake, body weight gain and feed conversion rate among the treatments. There were also no significant differences in the relative weight of breast meat, leg and liver. The abdominal fat tended to be decreased by the dietary AC. Significant differences were shown (P<0.05) in meat redness (a) and yellowness (b) among the treatments. However, the sensual characteristics of breast muscle was not affected by the dietary treatments. There were significant differences in total microbes, Coli forms and lactic acid bacteria (P<0.05) among the treatments. In experiment 2, a total of ninety, 40-wk-old Hy-line Brown laying hens were divided into 3 groups, consisting 3 replicates of 10 birds each and fed medicated control diet devoid of AC or diets containing 1% AC and 2% AC for 2 wks. The residue of antibiotics in Plasma and egg Yolk were significantly decreased (P<0.05) in 2% AC group compared to that of control. This study suggest that dietary AC may improve the external quality of edible meat and reduced antibiotics residue in chicken eggs without affecting laying performance.

Relationships between inbreeding coefficient and economic traits in inbred line of Duroc pigs (두록 계통조성 집단의 근교수준이 경제형질에 미치는 영향)

  • song, Na-Rae;Kim, Yong-Min;Kim, Doo-Wan;Sa, Soo-Jin;Kim, Ki-Hyun;Kim, Young-Hwa;Cho, Kyu-Ho;Do, Chang-hee;Hong, Joon-Ki
    • Korean Journal of Agricultural Science
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    • v.42 no.2
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    • pp.141-149
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    • 2015
  • The data of Duroc swine species that were born from 2000 to 2014 excluding missing ones collected by Korea National Institute of Animal Science were used in the present study. After removing missing data we used 9756 of productions data and 1728 of reproductive reference of breeding research to study the level of inbreeding and to investigate the impact on the reproductive traits, production traits. The correlation of reproductive traits and inbreeding coefficient are -0.07, -0.08 for total number pigs born, number of pigs born alive respectively and birth weight per litter is -0.10, number of pigs born alive per litter to 21days is -0.06 and body weight per litter to 21days is -0.09. The correlation coefficients of the inbreeding coefficients of reproductive traits are shown within 10% with negative correlation (P < 0.05). Days of 90kg and Backfat in the correlation coefficient and inbreeding coefficient production traits were not observed significant correlations, Average daily gain was investigated by the positive correlation of 0.05. According to the above results, the inbreeding level gave a negative effect on the improvement of the breed traits, investigating a relatively high compared to a negative effect on other traits. But overall correlation degree is less than 10% was observed. This inbreeding coefficient has not been clearly observed due to degeneration of the average inbreeding coefficients of these generations was maintained within 10% of the population. The scale of the experimental group was about 150 degree pig husbandry is very small compared to the advanced countries. However, the level of inbreeding in the population group with the appropriate mating combinations is maintained below 10% of population is thought to be small and can minimize the effects of inbreeding degeneration. further testing utilizing this selection is constantly considered to be necessary.

The Effect of Natural Mineral Complex Supplementation on Production, Egg Quality and Blood Characteristic in Laying Hens (사료 내 천연 복합 미네랄 제제의 첨가가 산란계의 생산성, 계란 품질 및 혈액 성상에 미치는 영향)

  • Yoo, J.S.;Kim, J.D.;Cho, J.H.;Chen, Y.J.;Kim, H.J.;Kang, D.K.;Min, B.J.;Kim, I.H.
    • Korean Journal of Poultry Science
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    • v.33 no.3
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    • pp.189-194
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    • 2006
  • This study was conducted to investigate the effects of natural mineral complex supplementation on egg production and characteristic in laying hens. A total of two hundred forty laying hens were randomly allocated into four treatments with ten replications for six weeks. Dietary treatments included 1) CON (control, basal diet) 2) M0.5 (basal diet + 3% chitosan + 0.5% natural mineral complex), 3) M1.0 (basal diet + 3% chitosan + 1.0% natural mineral complek) and 4) M1.5 (basal diet + 3% chitosan + 1.5% natural mineral complex). In the egg production, the M1.5 treatment was significantly higher than other treatments(P<0.05). However, egg weight was significantly higher in M0.5 treatment than CON and M1.0 treatments(P<0.05). Egg shell breaking strength was higher in M1.5 treatment than M0.5 treatment. Egg shell thickness was the highest in M1.5 treatment compared to other treatments(P<0.05). The hens 134 M0.5 diet were improved egg yolk color compared to those fed other diets(P<0.05). The Haugh unit, CON and M1.0 treatments showed significantly different results compared to those of M1.5 treatment(P<0.05). The M1.5 treatment had higher Ca and Fe concentration in blood and higher K concentration in yolk than CON(P<0.05). In conclusion, supplementation of natural mineral complex in laying hen diets influenced on egg production, egg shell qualify and mineral concentration in blood and yolk.

Linkage Analysis of the Resistance Genes to Whitebacked Planthopper (Sogatella furcifera Horvath) in Rice (수도의 흰등멸구(Sogatella furcifera Horvath)에 대한 저항성 유전자 연관분석)

  • ;Mun-Hue Heu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.2
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    • pp.136-151
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    • 1984
  • The purpose of this study is to find out the linkage relationship of the resistance genes Wbph1 and Wbph2 which are known to be present in the rice cultivar N22 and ARC 10239 respectively, with the genetic markers which are identified as the specific linkage tester. Crosses were made between the resistant parents and the genetic marker stocks and their F$_2$ populations were grown out in the field. The genetic segregations of the marker character were studied and the seeds were harvested individual plant base. These F$_3$ seeds were grown into plant-line base in the greenhouse and their responses to the whitebacked planthopper were tested. Then the linkage relationship between the F$_2$ plant marker character and the F$_3$ resistance responses to the whitebacked planthopper were examined. In the F$_2$ generation of the crosses between the resistant parent N22 and the genetic marker stocks, the genetic markers, such as lg, d-t, g, la, bl and gl, showed the segregation of 3 dominance to 1 recessiveness, and the Bh marker segregated into 9:7 ratio. Another 4 marker genes, such as Cl, gh, Lh and bc, did not show the good fittness to the expected value. In the F$_2$ generation of the crosses between the resistant parent ARC 10239 and the genetic marker stocks, the genetic markers, such as Cl, lg, Pn, g, la, bl and gl, showed the segregation of 3 dominance to 1 recessiveness, and the Bh gene segregation fitted well to the 9:7. The rest 4 genetic markers, such as gh, Lh, nl and be, did not show the good fitness to the expected ratio. The resistance genes Wbphl of N22 and the Wbph2 of ARC 10239 appeared to be single dominant gene each. The Wbphl gene was linked with the marker gene, liguleless (lg) of linkage group II with the recombination value of 36.8%, and with the black hull (Bh) with the value of 35.9%. The Wbph2 gene appeared to be independent of all the markers tested here, such as Cl, lg, Pn, g, Lh, la, nl, bl, bc, gl, Bh, of linkage gtoup I, II, III, IV, VI, VII, VIII, IX, X, XI, and XII respectively. That the Wbph2 linkage relations were not investigated was regarded as the causes that the tested marker genes on the chromosome were located with the resistance gene at the distant loci, and of the phenctypic properties of the marker characters. The Wbph2 linkage relations should be reexamined in the cross combinations of linkage group Ⅶ, Ⅷ, Ⅹ and XII including linkage group V which was not tested in this experiment.

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