• Title/Summary/Keyword: 3D culture system

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Regeneration from Storage Root Disk Culture of Purple Sweet Potato

  • Park, Hyejeong;Park, Hyeonyong
    • Korean Journal of Plant Resources
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    • v.28 no.3
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    • pp.363-369
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    • 2015
  • Sweet potato has low regeneration capacity, which is a serious obstacle for the fruitful production of transgenic plants. Simple and rapid regeneration method from storage root explants of purple sweet potato (Ipomoea batatas L.) was investigated. The embryogenic callus was observed from 4 cultivars and its highest rate was induced at 1 μM 2,4-D after 5 weeks of culture. Result revealed that a low concentration of 2,4-D and low light intensity was important factors for embryogenic callus formation. After subculture on medium with 5 μM ABA for 4 days, subsequently, occurred the regeneration of shoots within 4 weeks when these embryogenic callus was transferred onto the MS hormone free medium. Regenerated shoots were developed into platelets, and grown normal plants in the greenhouse. We developed a simple and quickly protocol to regenerate plantlets in storage root explants of purple sweet potato. This regeneration system will facilitate tissue culture and gene transfer research of purple sweet potato.

The Differentiation of bone Marrow Stromal Cells into NP-like Cells through 3-Dimensional Co-culture System (3차원 Co-culture 시스템을 통한 BMSC의 NP-like Cell로의 분화)

  • Kim, D.H.;Kim, S.H.;Heo, S.J.;Shin, J.W.;Kim, Y.J.;Park, S.H.;Jun, J.W.;Shin, J.W.
    • Journal of Biomedical Engineering Research
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    • v.29 no.2
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    • pp.159-163
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    • 2008
  • The goal of this study is to investigate the effect and potential of three-dimensional Co-culture of BMSCs (bone marrow stromal Cells) and NP (nucleus pulposus) Cells on the differentiation of BMSCs into NP-like Cells. The NP Cells and BMSCs were isolated and cultured from New Zealand White rabbits. The isolated NP Cells and BMSCs were prepared in different alginate beads. Those two types of beads were separated by a track-etched membrane of $3\;{\mu}m$ pore in a 6-well culture plate. No growth factors were used. In addition to these, NP and BMSC were cultured in the beads independently for control. The number of Cells in Co-culturing system was half of those in two control groups. Proliferation and production of glycosaminoglycan (GAG) were evaluated along with histological observation. The GAG production rate(GAG contents/Cell) of Co-cultured BMSCs were much higher than that of BMSCs cultured alone. The total amounts of GAG produced by BMSCs in Co-culturing system were larger than those produced by BMSCs in control group and were comparable with those produced by NP alone even the number of each Cell was half of BMSCs in Co-culturing system. This study showed the potential of differentiation of BMSCs into NP-like Cells through three-dimensional Co-culture system even without any chemical agents.

Augmented Reality Service Based on Object Pose Prediction Using PnP Algorithm

  • Kim, In-Seon;Jung, Tae-Won;Jung, Kye-Dong
    • International Journal of Advanced Culture Technology
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    • v.9 no.4
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    • pp.295-301
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    • 2021
  • Digital media technology is gradually developing with the development of convergence quaternary industrial technology and mobile devices. The combination of deep learning and augmented reality can provide more convenient and lively services through the interaction of 3D virtual images with the real world. We combine deep learning-based pose prediction with augmented reality technology. We predict the eight vertices of the bounding box of the object in the image. Using the predicted eight vertices(x,y), eight vertices(x,y,z) of 3D mesh, and the intrinsic parameter of the smartphone camera, we compute the external parameters of the camera through the PnP algorithm. We calculate the distance to the object and the degree of rotation of the object using the external parameter and apply to AR content. Our method provides services in a web environment, making it highly accessible to users and easy to maintain the system. As we provide augmented reality services using consumers' smartphone cameras, we can apply them to various business fields.

Development of a Pattern and Visual Image for a One-Piece Dress using a 3D Virtual Clothing System (3D 가상 착의 시스템을 활용한 원피스 드레스의 원형 개발 및 시각적 이미지 연구)

  • Uh, Mi-Kyung
    • The Research Journal of the Costume Culture
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    • v.19 no.3
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    • pp.597-611
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    • 2011
  • The purpose of this study is to propose a design method for one-piece dress patterns with high body fitness through an appearance evaluation of one-piece dress patterns with the application of the 3D Virtual Clothing System known as i-designer. Measures consisted of an optical illusion effect in visual imagery and mutual influence according to a change in the length and princess line, in a silhouette of a one-piece dress. The data was assessed by a t-test and a multi-way ANOVA and factor analysis using SPSS 17.0. The results were as follows; The design of the study pattern was done by modifying the ease of the bust circumference, ease of the abdominal circumference, ease of the hip circumference, the position of the side seam line, and the appearance of horizontality in the hem line, which received a low evaluation in appearance evaluations of a one-piece dress pattern. As a result of analyzing the visual image according to a change in the length and princess line, in the form of a silhouette of a one-piece dress, four factors were selected; the whole-body optical illusion factor, the upper-body optical illusion factor, the bust optical illusion factor, and the lower-body optical illusion factor. As a result of analyzing the effect of the interaction in the visual image according to the design variables, the influence of the main effect was found to be great in each factor. In the upper-body optical illusion factor, a significant difference was not noted in the two-way interaction between the length and the princess line. However, the influence on three-way interaction among the length, princess line, and silhouette was significant.

Development of Control Method for Self-Driving Roller Conveyor Based on 3D Simulation (자체 구동 롤러 컨베이어의 3차원 시뮬레이션 기반 제어 기법 개발)

  • Seokwon Lee;Byungmin Kim;Heon Huh
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.3
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    • pp.861-864
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    • 2024
  • The self-driving roller conveyor system, which transports target products by controlling multiple rollers with a motor, is a logistics system suitable for branching and joining logistics and controlling the alignment of target products, and its utilization is increasing, especially in the food manufacturing process. In this paper, we build a simulation environment using Unity software based on 3D graphic modeling of a self-driving roller conveyor system. In a situation where target products are supplied irregularly in terms of time, a method is proposed that can align products to maintain constant spacing by controlling the rollers. Simulation results show that effective alignment of products is possible by controlling the motor that drives the roller based on sensor data of the product position.

Mass Propagation of Dicentra spectabilis L. Lemaire Through In vitro Suspension Culture (현탁배양을 통한 금낭화(Dicentra spectabilis L. Lemaire)의 대량증식)

  • Lee, Kang-Seop;Sim, Ock-Kyeong;Shin, Jeong-Sun;Choi, Yong-Eui;Kim, Ee-Yup
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.121-126
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    • 2004
  • Bleeding heart (Dicentra spectabilis L. Lemaire) is one of the most valuable wild flower in Korea. This work was conducted for the mass production of somatic embryos through suspension culture and more effective plant regeneration system in Dicentra spectabilis. High-frequency embryogenic callus proliferation was achieved in SH liquid medium supplemented with 1 mg/L 2,4-D. Half-strength SH medium was suitable concentration for somatic embryo induction and germination. About 5,000 embryos were produced per 250$m\ell$ flask after 4 weeks of culture. Germination rate of somatic embryos was decreased when GA$_3$ was added in medium. The plantlets showed a 58% survival rate when transferred to pots after 1 month of culture. The results indicate that micropropagation procedure via somatic embryogenesis can be applied for an efficient mass propagation of Dicentra spectabilis.

ICT Convergenced Cascade-type Incubator for mass production of microalgae (미세조류 대량생산을 위한 ICT 융합 계단식 연속 배양 장치)

  • Lee, Geon Woo;Lee, Yong Bok;Yoo, Yong Jin;Baek, Dong Hyun;Kim, Jin Woo;Kim, Ho Seob
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.5
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    • pp.379-386
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    • 2021
  • This study was undertaken to develop a cascade-type continuous culture system (CCCS) that combines both ICT and biotechnology (BT), for the mass production of microalgae. This system is capable of maintaining the essential culture conditions of pH, temperature, carbon dioxide, and illuminance control, which are key parameters for the growth of microalgae, and is economical for producing microalgae regardless of the season or location. It has the added advantage of providing stable and high productivity. In the current study, this system was applied to culture microalgae for 71 days, with subsequent analysis of the experimental data. The initial O.D. of the culture measured from incubator 1 was 0.006. On the 71st day of culture, the O.D.s obtained were 0.399 (incubator 1), 0.961 (incubator 2), 0.795 (incubator 3), and 0.438 (incubator 4), thereby confirming the establishment of continuous culture. Thus, we present a smart-farm based on ISMC (in-situ monitoring and control) for a mass culture method. We believe that this developed technology is suitable for commercialization, and has the potential to be applied to hydroponics-based cultivation of microalgae and cultivation of high-value-added medicinal plants as well as other plants used in functional foods, cosmetics, and medical materials.

Automatic 3D data extraction method of fashion image with mannequin using watershed and U-net (워터쉐드와 U-net을 이용한 마네킹 패션 이미지의 자동 3D 데이터 추출 방법)

  • Youngmin Park
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.3
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    • pp.825-834
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    • 2023
  • The demands of people who purchase fashion products on Internet shopping are gradually increasing, and attempts are being made to provide user-friendly images with 3D contents and web 3D software instead of pictures and videos of products provided. As a reason for this issue, which has emerged as the most important aspect in the fashion web shopping industry, complaints that the product is different when the product is received and the image at the time of purchase has been heightened. As a way to solve this problem, various image processing technologies have been introduced, but there is a limit to the quality of 2D images. In this study, we proposed an automatic conversion technology that converts 2D images into 3D and grafts them to web 3D technology that allows customers to identify products in various locations and reduces the cost and calculation time required for conversion. We developed a system that shoots a mannequin by placing it on a rotating turntable using only 8 cameras. In order to extract only the clothing part from the image taken by this system, markers are removed using U-net, and an algorithm that extracts only the clothing area by identifying the color feature information of the background area and mannequin area is proposed. Using this algorithm, the time taken to extract only the clothes area after taking an image is 2.25 seconds per image, and it takes a total of 144 seconds (2 minutes and 4 seconds) when taking 64 images of one piece of clothing. It can extract 3D objects with very good performance compared to the system.

Comparison and analysis of men's classic-fit and slim-fit shirt patterns - Utilizing the 3D virtual try-on system - (남성의 클래식핏과 슬림핏 셔츠 패턴 비교 분석에 관한 연구 - 3D 가상착의를 활용하여 -)

  • Kim, Minjung;Kim, Myoung-Ok;Park, Sunkyung
    • The Research Journal of the Costume Culture
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    • v.22 no.2
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    • pp.209-224
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    • 2014
  • This study aims to compare the ease of shirt patterns in the men's wear market that have been constantly developing, and to analyze the fitness and appearance through the 3D virtual try-on system. The study selected three industrial patterns and two educational materials of classic-fit and slim-fit shirts for a total of ten items. The experiments involved virtual wearing, a stress contact point perspective map, and appearance evaluations. First, the ease differed significantly in the chest, waist, and sleeve cap height according to the patterns. Second, based on the stress, contact point, and transparency, there was ease in the order of Nam, D-brand, J-brand, Park, and S-brand in the classic-fit shirts while the order of D-brand, Nam, J-brand, Park, and S-brand resulted in the slim-fit shirts. Third, in the appearance evaluation, higher points resulted in the order of J-brand, Park, S-brand, D-brand, and Nam in both classic-fit and slim-fit. The results showed that proper ease differs greatly by shirt-fit and target age. Also, the shirts with more ease tended to be evaluated less favorably in the appearance evaluation. Based on the results, the study suggests that the proper ease in chest circumference is 18cm for the classic-fit shirts and 6 cm for the slim-fit shirts.