DOI QR코드

DOI QR Code

The Differentiation of bone Marrow Stromal Cells into NP-like Cells through 3-Dimensional Co-culture System

3차원 Co-culture 시스템을 통한 BMSC의 NP-like Cell로의 분화

  • Kim, D.H. (Dept. of Biomedical Engineering, Inje University) ;
  • Kim, S.H. (Team of BK21 / First Research Team, Institute of Biomedical Engineering, Inje University) ;
  • Heo, S.J. (Dept. of Biomedical Engineering, Inje University) ;
  • Shin, J.W. (Dept. of Biomedical Engineering, Inje University) ;
  • Kim, Y.J. (Dept. of Dental Lab. Science, Catholic University of Pusan) ;
  • Park, S.H. (Dept. of Biomedical Engineering, Inje University) ;
  • Jun, J.W. (Dept. of Biomedical Engineering, Inje University) ;
  • Shin, J.W. (Dept. of Biomedical Engineering, Inje University)
  • 김동화 (인제대학교 의용공학과) ;
  • 김수향 (인제대학교 의용공학과 BK21 사업단 / FIRST 연구단) ;
  • 허수진 (인제대학교 의용공학과) ;
  • 신지원 (인제대학교 의용공학과) ;
  • 김영직 (부산가톨릭대학교 치기공학과) ;
  • 박소희 (인제대학교 의용공학과) ;
  • 전재우 (인제대학교 의용공학과) ;
  • 신정욱 (인제대학교 의용공학과)
  • Published : 2008.04.30

Abstract

The goal of this study is to investigate the effect and potential of three-dimensional Co-culture of BMSCs (bone marrow stromal Cells) and NP (nucleus pulposus) Cells on the differentiation of BMSCs into NP-like Cells. The NP Cells and BMSCs were isolated and cultured from New Zealand White rabbits. The isolated NP Cells and BMSCs were prepared in different alginate beads. Those two types of beads were separated by a track-etched membrane of $3\;{\mu}m$ pore in a 6-well culture plate. No growth factors were used. In addition to these, NP and BMSC were cultured in the beads independently for control. The number of Cells in Co-culturing system was half of those in two control groups. Proliferation and production of glycosaminoglycan (GAG) were evaluated along with histological observation. The GAG production rate(GAG contents/Cell) of Co-cultured BMSCs were much higher than that of BMSCs cultured alone. The total amounts of GAG produced by BMSCs in Co-culturing system were larger than those produced by BMSCs in control group and were comparable with those produced by NP alone even the number of each Cell was half of BMSCs in Co-culturing system. This study showed the potential of differentiation of BMSCs into NP-like Cells through three-dimensional Co-culture system even without any chemical agents.

Keywords

References

  1. Butler D, Trafimow JH, and Andersson GB, 'Disc degenerate before facets,' Spine, vol. 15, pp.111-113, 1990 https://doi.org/10.1097/00007632-199002000-00012
  2. Lipson SJ and Muir H, 'Proteoglycans in experimental intervertebral disc degeneration,' Spine, vol. 6, pp.194-210, 1981 https://doi.org/10.1097/00007632-198105000-00002
  3. Gruber HE, Johnson TL, and Leslie K, 'Autologous intervertebral disc Cell implantation: a model using Psammomys obesus, the sand rat,' Spine, vol. 27, pp.1626-1633, 2002 https://doi.org/10.1097/00007632-200208010-00007
  4. Mizuno H, Roy AK, and Vacanti CA, 'Tissue-engineered composites of anulus fibrosus and nucleus pulposus for intervertebral disc replacement,' Spine, vol. 29, pp.1290-1297, 2004 https://doi.org/10.1097/01.BRS.0000128264.46510.27
  5. Maroudas A, Stockwell RA, and Nachemson A, 'Factors involved in the nutrition of the human lumber intervertebral disc: Cellularity and diffusion of glucose in vitro,' J Anat., vol. 120, pp.113-130, 1975
  6. Stephen M, Richardson, and Rachael V, 'Intervertebral Disc Cell-Mediated Mesencymal Stem Cell Differentiation,' Stem cells, vol. 24, pp.707-716, 2006 https://doi.org/10.1634/stemcells.2005-0205
  7. Steck E, Bertram H, and Abel R, 'Induction of Intervertebral Disc-Like Cells From Adult Mesenchymal Stem Cells,' Stem cells, vol. 23, pp.403-411, 2005 https://doi.org/10.1634/stemcells.2004-0107
  8. Risbud MV, Albert TJ, and Guttapalli A, 'Differentiation of mesenchymal stem Cells towards a nucleus pulposus-like phenotype in vitro: Implications for Cell-based transplantation therapy,' Spine, vol. 29, pp.2627-2632, 2004 https://doi.org/10.1097/01.brs.0000146462.92171.7f
  9. Aguiar DJ, Johnson SL, and Oegema TR, 'Notochordal Cells Interact with Nucleus Pulposus Cells: Regulation of Proteoglycan Synthesis,' Exp Cell Res., vol. 246, pp.129-137, 1999 https://doi.org/10.1006/excr.1998.4287
  10. Yamamoto Y, Mochida J, and Sakai D, 'Upregulation of the Viability of Nucleus Pulposus Cells by Bone Marrow-Derived Stromal Cells. Significance of Direct Cell-to-Cell Contact in Coculture System,' Spine, vol. 29, pp.1508-1514, 2004 https://doi.org/10.1097/01.BRS.0000131416.90906.20
  11. Aulthous AL, Beck M, and Griffey E, 'Expression of the human chondrocyte phenotype in vitro,' In Vitro Cell Dev Biol., vol. 25, pp.659-668, 1989 https://doi.org/10.1007/BF02623638
  12. Archer CW, McDowell J, and Bayliss MT, 'Phenotypic modulation in sub-populations of human articular chondrocytes in vitro,' J Cell Sci., vol. 97, pp.361-371, 1990
  13. Kimura T, Yasui N, and Ohsawa S, 'Chondrocytes embedded in collagen gels maintain cartilage phenotype during long-term cultures,' Clin Orthop., vol. 186, pp.231-239, 1984
  14. Bassleer C, Gysen P, and Foidart JM, 'Human chondrocytes in tridimensional culture,' In Vitro Cell Dev Biol., vol. 22, pp. 113-119, 1986 https://doi.org/10.1007/BF02623497
  15. Beekman B, Verzij N, and Bank RA, 'Synthesis of collagen by bovine chondrocytes cultured in alginate; posttranslational modifications and Cell-matrix interaction,' Exp Cell Res., vol. 237, pp.135-141, 1997 https://doi.org/10.1006/excr.1997.3771
  16. Richardson SM, Curran JM, and Chen R, 'The differentiation of bone marrow mesenchymal stem Cells into chondrocyte-like Cells on poly-L-lactic acid (PLLA) scaffolds,' Biomaterials., vol. 27, pp.4069-4078, 2006 https://doi.org/10.1016/j.biomaterials.2006.03.017