Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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The Differentiation of bone Marrow Stromal Cells into NP-like Cells through 3-Dimensional Co-culture System

3차원 Co-culture 시스템을 통한 BMSC의 NP-like Cell로의 분화

  • Kim, D.H. (Dept. of Biomedical Engineering, Inje University) ;
  • Kim, S.H. (Team of BK21 / First Research Team, Institute of Biomedical Engineering, Inje University) ;
  • Heo, S.J. (Dept. of Biomedical Engineering, Inje University) ;
  • Shin, J.W. (Dept. of Biomedical Engineering, Inje University) ;
  • Kim, Y.J. (Dept. of Dental Lab. Science, Catholic University of Pusan) ;
  • Park, S.H. (Dept. of Biomedical Engineering, Inje University) ;
  • Jun, J.W. (Dept. of Biomedical Engineering, Inje University) ;
  • Shin, J.W. (Dept. of Biomedical Engineering, Inje University)
  • 김동화 (인제대학교 의용공학과) ;
  • 김수향 (인제대학교 의용공학과 BK21 사업단 / FIRST 연구단) ;
  • 허수진 (인제대학교 의용공학과) ;
  • 신지원 (인제대학교 의용공학과) ;
  • 김영직 (부산가톨릭대학교 치기공학과) ;
  • 박소희 (인제대학교 의용공학과) ;
  • 전재우 (인제대학교 의용공학과) ;
  • 신정욱 (인제대학교 의용공학과)
  • Published : 2008.04.30
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Abstract

The goal of this study is to investigate the effect and potential of three-dimensional Co-culture of BMSCs (bone marrow stromal Cells) and NP (nucleus pulposus) Cells on the differentiation of BMSCs into NP-like Cells. The NP Cells and BMSCs were isolated and cultured from New Zealand White rabbits. The isolated NP Cells and BMSCs were prepared in different alginate beads. Those two types of beads were separated by a track-etched membrane of $3\;{\mu}m$ pore in a 6-well culture plate. No growth factors were used. In addition to these, NP and BMSC were cultured in the beads independently for control. The number of Cells in Co-culturing system was half of those in two control groups. Proliferation and production of glycosaminoglycan (GAG) were evaluated along with histological observation. The GAG production rate(GAG contents/Cell) of Co-cultured BMSCs were much higher than that of BMSCs cultured alone. The total amounts of GAG produced by BMSCs in Co-culturing system were larger than those produced by BMSCs in control group and were comparable with those produced by NP alone even the number of each Cell was half of BMSCs in Co-culturing system. This study showed the potential of differentiation of BMSCs into NP-like Cells through three-dimensional Co-culture system even without any chemical agents.

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