• Journal of the Optical Society of Korea
• /
• 제16권1호
• /
• pp.42-46
• /
• 2012

We have developed a fluorescence optical detection system using a digital micromirror device (DMD) for monitoring 3D cell culture matrices in situ. Full 3D imaging with fast scanning speed was implemented by the combined action of a DMD and a motorized stage. Imaging results with fluorescent microbeads measure the minimum axial resolution of the system as $6.3{\mu}m$, while full 1-mm scanning through 3D alginate-based matrix was demonstrated. For cell imaging, improved images were obtained by removing background fluorescence although the scanning distance was reduced because of low intracellular fluorescence efficiency. The system is expected to be useful to study various dynamics and behaviors of 3-dimensionally cultured cells in microfluidic systems.

• 한국자원식물학회지
• /
• 제24권3호
• /
• pp.280-285
• /
• 2011

In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, ${\alpha}$-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), $N^6$-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.

• Journal of Biosystems Engineering
• /
• 제24권1호
• /
• pp.41-50
• /
• 1999

This study aims at detecting the three dimensional gripping points for the transplanting robot system to grip in the process of developing transplanting robot system, which is one of the automation systems for transplanting tissue culture. The stereo vision system equipped with two cameras has been used to detect the gripping points of the plant stem. The method for matching the plants of the image information which came from two cameras was to measure the total numbers of pixels, leaves, and the heights of the plants. The gripping points were detected near the roots after extracting the stem parts by the standard deviation of the X axis according to the Y axis. The performance test of the developed program showed that the detecting errors of the gripping points were 0∼1mm for X axis and 1∼2mm for Y & Z axis. The mean running time of the program was about 3 seconds.

• International Journal of Advanced Culture Technology
• /
• 제9권4호
• /
• pp.302-306
• /
• 2021

Indoor localization is one of the basic elements of Location-Based Service, such as indoor navigation, location-based precision marketing, spatial recognition of robotics, augmented reality, and mixed reality. We propose a Voxel Labeling-based visual positioning system using object simultaneous localization and mapping (SLAM). Our method is a method of determining a location through single image 3D cuboid object detection and object SLAM for indoor navigation, then mapping to create an indoor map, addressing it with voxels, and matching with a defined space. First, high-quality cuboids are created from sampling 2D bounding boxes and vanishing points for single image object detection. And after jointly optimizing the poses of cameras, objects, and points, it is a Visual Positioning System (VPS) through matching with the pose information of the object in the voxel database. Our method provided the spatial information needed to the user with improved location accuracy and direction estimation.

• 한국작물학회지
• /
• 제49권3호
• /
• pp.232-236
• /
• 2004

오차드그래스의 최적 조직배양조건을 확립하기 위하여 'Roughrider' 품종의 성숙종자로부터 최적 배발생 캘러스 유도 및 식물체 재분화에 미치는 배지첨가물질의 영향을 조사하였다. 성숙종자로부터 배발생 캘러스 유도시 첨가되는 생장조절 물질로는 3 mg/L 2,4-D가 가장 효율적이었으며, 식물체 재분화에는 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 배지에 캘러스를 배양했을 때 36.3%의 재분화율을 나타내었다. 캘러스 유도배지와 재분화배지에 1 g/L의 casein hydrolysate와 300 mg/L의 proline을 동시에 첨가해주었을 때 캘러스 유도율과 재분화율이 각각 57.3%와 60.7%로 증가되었다. 또한 항산화물질로서 10 mg/L의 $\textrm{AgNO}_3$ 와 40 mg/L의 cysteine을 첨가해준 과 켈러스 유도율 재분화율 각각 68.7%와 71.7%까지 증가되었다. 본 연구를 통하여 확립된 성숙종자로부터 효율적인 배발생 캘러스의 유도 및 식물체 재분화 체계는 유전자 형질전환을 통한 신품종 오차드그래스 개발에 유용하게 응용되어질 수 있을 것이다.

• 한국멀티미디어학회논문지
• /
• 제24권10호
• /
• pp.1346-1357
• /
• 2021

The drone technology, which is receiving a lot of attention due to the 4th industrial revolution, requires an Unmanned Aerial Vehicles'(UAVs) flight path search algorithm for automatic operation and driver assistance. Various studies related to flight path prediction and recommendation algorithms are being actively conducted, and many studies using the A-Star algorithm are typically performed. In this paper, we propose an Optimal 3D Flight Path Recommendation System for unmanned aerial vehicles. The proposed system was implemented and simulated in Unity 3D, and by indicating the meaning of the route using three different colors, such as planned route, the recommended route, and the current route were compared each other. And obstacle response experiments were conducted to cope with bad weather. It is expected that the proposed system will provide an improved user experience compared to the existing system through accurate and real-time adaptive path prediction in a 3D mixed reality environment.

• 복식
• /
• 제56권5호
• /
• pp.29-43
• /
• 2006

The purpose of this study is to examine a unique characteristic of the colors of the costumes in Korean basic culture in the aim of seeking the characteristics and the conceptual meanings of colors found in the majority’s culture. The scope of the basic culture was divided into folk belief, folk game and folk play. Within these limits, the colors of the dress, accessories, instruments were extracted by comparing with the naked eye in NCS Color System. For the analysis of hue and tone, the secondary dimensional analysis using NCS color system and the three-dimensional analysis using the software, COLOR 3D Version 2.0, were done. The result of this investigation is that the colors of the costume in the Korean basic culture are white, gray and black of achromatic color and yellow, yellowish red and purplish blue. This confirms that the colors based on Five-elements color are becoming the basis too basic culture. And Arche-pattern, which is a characteristic commonly found in the Korean traditional society, was shown as a characteristic of color. The colors of the costumes in Korean basic culture are uniquely adopted by the Korean civilians according to their religious and philosophical living standard. This study is meaningful in seeking a root for the formation of their unique color culture.

• International Journal of Oral Biology
• /
• 제30권1호
• /
• pp.23-30
• /
• 2005

Bone remodeling is a process controlled by the action of two major bone cells; the bone forming osteoblast and the bone resorbing osteoclast. In the process of osteoclastogenesis, stromal cells and osteoblast produce RANKL, OPG, and M-CSF, which in turn regulate the osteoclastogenesis. During the bone resorption by activated osteoclasts, extracellular $Ca^{2+}/{PO_4}^{2-}$ concentration and degraded organic materials goes up, providing the hypertonic microenvironment. In this study, we tested the effects of hypertonicity due to the degraded organic materials on osteoclastogenesis in co-culture system. It was examined the cellular response of osteoblastic cell in terms of osteoclastogenesis by applying the sucrose, and mannitol, as a substitute of degraded organic materials to co-culture system. Apart from the sucrose, mannitol, and NaCl was tested to be compared to the effect of organic osmotic particles. The addition of sucrose and mannitol (25, 50, 100, 150, or 200 mM) to co-culture medium inhibited the number of tartrate-resistant acid phosphatase (TRAP) positive multinucleated cells induced by 10 nM $1{\alpha},25(OH)_2vitaminD_3$ ($1{\alpha},25(OH)_2D_3$). However, NaCl did exert harmful effect upon the cells in this co-culture system, which is attributed to DNA damage in high concentration of NaCl. To further investigate the mechanism by which hypertonicity inhibits $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis, the mRNA expressions of receptor activator of nuclear factor (NF)-kB ligand (RANKL) and osteoprotegerin (OPG) were monitored by RT-PCR. In the presence of sucrose (50 mM), RANKL mRNA expression was decreased in a dose-dependent manner, while the change in OPG and M-CSF mRNA were not occurred in significantly. The RANKL mRNA expression was inhibited for 48 hours in the presence of sucrose (50 mM), but such a decrement recovered after 72 hours. However, there were no considerable changes in the expression of OPG and M-CSF mRNA. Conclusively, these findings strongly suggest that hypertonic stress down-regulates $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis via RANKL signal pathway in osteoblastic cell, and may playa pivotal role as a regulator that modulates osteoclastogenesis.

• 문화기술의 융합
• /
• 제8권3호
• /
• pp.469-475
• /
• 2022

딥러닝 기반 1인 화자 TTS 시스템의 모델을 학습하기 위해서 수십 시간 분량의 음성 DB와 많은 학습 시간이 요구된다. 이것은 다화자 또는 개인화 TTS 모델을 학습시키기 위해서는 시간과 비용 측면에서 비효율적 방법이다. 음색 복제 방법은 새로운 화자의 TTS 모델을 생성하기 위하여 화자 인코더 모델을 이용하는 방식이다. 학습된 화자 인코더 모델을 통해 학습에 사용되지 않은 새로운 화자의 적은 음성 파일로부터 이 화자의 음색을 대표하는 화자 임베딩 벡터를 만든다. 본 논문에서는 음색 복제 방식을 적용한 다화자 TTS 시스템을 제안한다. 제안한 TTS 시스템은 화자 인코더, synthesizer와 보코더로 구성되어 있는데, 화자 인코더는 화자인식 분야에서 사용하는 d-vector 기법을 적용한다. 학습된 화자 인코더에서 도출한 d-vector를 synthesizer에 입력으로 추가하여 새로운 화자의 음색을 표현한다. MOS와 음색 유사도 청취 방법으로 도출한 실험 결과로부터 제안한 TTS 시스템의 성능이 우수함을 알 수 있다.

• 복식문화연구
• /
• 제19권2호
• /
• pp.245-254
• /
• 2011

The purpose of this study was to develop uniform pattern of obese junior-high school girls by using the virtual twin and 3D virtual garment simulation system. The results were as follows; first, by using 3D virtual garment simulation, new uniform pattern considered obese junior-high school girls was development. The basic numerical formula were as follows; bust girth=B/2+2.5, armhole depth=B/4, front waist girth=W/4+1.8, back waist girth=W/4+1, front hip girth=H/4+1, back hip girth=H/4+1, chest width=chest width+1.5, back width=back width+1 and back neck width 8cm. Second, according to the results of the new uniform pattern's appearance evaluation, it estimated more highly than existing pattern in silhouette and ease amount, confirming that new uniform pattern is appropriate for the obese junior-high school girls. Also, new uniform pattern was evaluated to allow proper space length of bust, waist, abdomen and hip. Virtual models production through 3D body scan data, pattern draft and virtual garment digital program were applied to prototypic design method so as to enhance the fitness of ready-made garments. This study is expected to serve as one of important basic data for ensuing studies that may utilize 3D virtual garment simulation system with 2D patterns, and also for future 3D pattern production program development.