• The Korean Journal of Physiology and Pharmacology
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• 제14권3호
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• pp.169-176
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• 2010

The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects $1{\alpha}$, 25-dihydroxyvitamin $D_3$ ($1{\alpha},25(OH)_2D_3$)-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of $1{\alpha},25(OH)_2D_3$-induced tartrate-resistant acid phosphatase-positive multinucleated cells and $1{\alpha},25(OH)_2D_3$-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) and Runx2 expressions were down-regulated in response to $1{\alpha},25(OH)_2D_3$. Knockdown of Runx2 inhibited $1{\alpha},25(OH)_2D_3$-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells.

• Journal of Microbiology
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• 제43권3호
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• pp.257-259
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• 2005

Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (BPA) and anaerobic (BPN) media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in $5\%$ sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar. Organisms growing on these subcultures were subsequently identified using both Vitek32 (bioMerieux, Durham, NC) and conventional methods. Twenty four $(2.6\%)$ of the 904 subcultures grew on the subculture media. The majority $(83.3\%)$ of these were determined to be gram-positive microorganisms. Fourteen $(58.3\%)$ were coagulase-negative staphylococci, two $(8.3\%)$ were Bacillus spp., one $(4.2\%)$ was Staphylococcus aureus, and one $(4.2\%)$ was identified as Enterococcus faecium. Streptococcus pneumoniae and Neisseria spp. were isolated together in two $(8.3\%)$ vials. Gram-negative microorganisms comprised $12.5\%$ of the subcultures, of which two $(8.3\%)$ were found to be Pseudomonas aeruginosa, and one $(4.2\%)$ was Pseudomonas fluorescens. The other isolate $(4.2\%)$ was identified as Candida albicans. We conclude that the subculture of negative results is valuable in the BacT/Alert 3D system, especially in situations in which only one set of blood cultures is taken.

• 복식문화연구
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• 제8권4호
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• pp.587-601
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• 2000

The purpose of this study : 1. The total 56 range measurements of active dynamic motion of 40 subjects (20's and 30's) were measured using 3-D dynamic motion analysis system. 2. Various comparisons were performed for the right and left side, male, age groups (20's, 30's, and 40's ∼ 60's) using previous studies. The results were compared with the other studies in the aspects of age. In this study, the 3-D motion analysis system based on photogrammetry was established and used to analyze the human's motion and posture. The system consists of VICON 140, data acquisition system, and data analysis program (KRISSMAS). The result of this study were as follows : 1. Comparing 20's with 30's the result shows that 30's have larger ROM at some joints, which is inconsistent with the previous result. The reason is that female subjects in 20's were improperly sampled according to the representatives of anthropometry characteristics. 2. There are significant differences in some joints related with age. 20's male subjects have more flexible joints in the neck while 30's male subjects have more flexibility in their shoulder joint and elbow joint. But most of the significances were not high (p〈0.05). The prediction that the right side of Korean bodies would be more flexible was not a good hypothesis. And the joints flexibilities are not correlated with Rohrer's Index.

• 한국산학기술학회논문지
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• 제18권10호
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• pp.551-558
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• 2017

조직특이적 프로모터 및 벡터를 연구하고 검증하기 위해서는 조직 및 종의 특이성을 유지하는 세포 시스템을 개발하는 것이 바람직하다. 이러한 시스템은 형질전환동물 모델에 대한 효과적인 대안이다. 우리는 베타 카제인 (CSN2)의 세포 형태와 mRNA 수준에 기초하여 일차 배양으로부터 돼지 유선 상피 세포 주 (PMECs)를 확립하였다. 선택된 PMECs는 cytokeratin 항체에 의해 염색되었으며, 유선 상피 세포에 존재한다고 생각되어지는 유즙 단백질 유전자 (CSN2, 락토페린 및 유청 단백질)를 발현하는 것으로 나타났다. 또한, 3D 배양에서 PMECs932-7의 acini 구조를 확인하기 위해 살아있는 세포를 핵산에 결합하는 SYTO-13으로 염색하였다. 우리는 마트리겔 (matrigel)에 있는 PMECs의 acini가 말초 세포의 응집에 의해 형성되고 공간의 lumen을 특징으로 한다는 것이 관찰하였다. 우리는 PMECs의 matrigel 사용법과 세포 밀도를 포함한 세포 배양 조건의 영향을 시험함으로서 시스템을 시연했다. 이러한 결과는 PMCEs의 유선 상피 세포는 유전적 또는 구조적 특징을 갖고 있음을 시사하고 있다.

• 복식문화연구
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• 제8권5호
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• pp.741-753
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• 2000

The purpose of this study : 1. The total 24 range measurements of active dynamic motion of 40 subjects(20's and 30's, and 40's∼60's) using previous studies. The results were compared with the other studies in the aspects of age. In this study, The 3-D motion analysis system consists of VICON 140, data acquisition system, and data analysis program called by KRISSMAS. VICON 140 is dynamic motion analyzer, and KRISSMAS is a program developed for analysis of the data captured from the VICON. The results of this study were as follows : 1. Comparing 20's∼30's and 40's∼60's the result shows that 40's∼60's have smaller ROM at the most joints(Thoratic and Lumber, Hip Joint, Knee Joint, Ankle Joint), which is inconsistent with the previous result. The reason is that most investigations were carried out using traditional measurement equipments, which have encountered difficulty in quantifying the exact motion of the body. 2. There are no significant mean difference between the right and the left side. Most of the significances were not high(〉0.05).

• International Journal of Advanced Culture Technology
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• 제10권4호
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• pp.478-486
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• 2022

Holography is a human-friendly technology that can express 3D stereoscopic information without eye fatigue. You can experience the mysterious features of light directly and indirectly, and you can also experience the principles of 3D imaging, which makes it a very good curriculum. As such, although holography is very effective in teaching students optics and 3D imaging technology, it has not yet been systematically established. The reason is the cost burden such as expensive equipment and laboratories, and the lack of easily accessible educational equipment. We implemented a portable holographic recording system to solve this problem. In addition, since silver halides, which use harmful chemicals, are not used in the process of developing the recording medium, and photopolymers are used, it is possible to educate not only the general public but also young students. In order to improve the completeness of the recorded result, the mechanism part, light source, and recording medium part of the production system were newly constructed to complement all the existing problems. The proposed system will make holography easily accessible to many people in a variety of fields, not just education. Through the interesting experience of various features and principles of light and the production of holograms with high satisfaction, we hope to popularize them in various fields such as education.

• 문화기술의 융합
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• 제7권1호
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• pp.75-81
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• 2021

첨단 정보통신 기술의 발달에 따라 디지털 문화에 익숙한 Z세대는 미래 패션을 재편할 수 있는 패션 산업의 주요 소비층으로 관심이 집중되고 있다. 본 연구에서는 디지털 기기에 능숙하고 인터넷을 통한 정보 습득 및 모바일 라이프스타일에 친숙한 Z세대를 위한 캐쥬얼웨어를 3D 가상 시뮬레이션에 의해 디자인하여 걸리쉬, 스포티브, 이지, 컨템포러리의 4가지 룩으로 디자인 전개하였다. 의상디자인에 3D 가상 착의 시뮬레이션의 활용은 팬데믹 환경에서 디지털 패션 디자인기획 및 생산 프로세스와의 융합을 통한 미래 패션 산업의 디지털화를 구축하고 온라인 플랫폼의 유통을 강화시킬 수 있다. 언택트 패션 제작 시스템을 도입하여 업무의 효율성 강화를 위한 지속적인 혁신을 시도하고 있는 기업 환경에서 3D 가상의상 기술의 활용은 3D 샘플 제작, 온라인 피팅, 수정과 보정 및 품평을 통해 업무 프로세스에서 소통이 확대되고 인적, 물적 자원의 경감에 따른 긍정적 효과를 통해 지속 가능한 패션의 가치를 확대시킬 수 있다.

• 생명과학회지
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• 제7권4호
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• pp.377-383
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• 1997

The acute toxicity effect of triorganotin of trioganotin on the growth of microalgae and shellfish was investigated through flask culture. The value of 120 hr-LC$_{50}$ that is the median lethal concentration of TBTO on the shellfish (R. philipinarum) was found to be 6 $\mu$g/L. The acute toxicity effect of TBTO on T. suecica was obviously shown even at the concentration of 0.5 $\mu$g/L, and the effect diminished as the initial cell density increased. The effect also diminished less in the experiment done under aeration than in that done under non-aeration. To design a chemostat system for the test of chronic toxicity, the culture of T. suecica was executed in photobioreactor. In batch culture, the profiles of chlorophyII a and D.C.W. showed the growth of T. suecica very well, and the maximum specific growth rate was estimated to be 0.54 d$^{-1}$. with this value, as a dilution rate in contimuous culture, pH was nicely maintained between 7 and 9 when air was supplied with 3% CO$_{2}$. From all results and the natural environment of clam, a novel chemostat system was invented. Through this system, we can observe each independent toxicity effect of TBTO and plankton and combined toxicity effect as well.

• 문화기술의 융합
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• 제4권4호
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• pp.309-316
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• 2018

본 연구에서는 실제와 같은 가상발표환경을 구현하여 실전에서 자신감 있게 발표할 수 있도록 도와주기 위한 3D 가상현실기반의 발표훈련시스템을 제안한다. 제안시스템은 발표자의 음성과 행동을 실시간으로 분석하여 가상공간의 청중들에게 반영되게 함으로서 사실감 있고 몰입도 높은 발표 및 면접 환경을 제공하였다. 발표자는 6DOF Tracking이 되는 HMD와 VR Controller를 착용하고 Kinect를 이용하여 가상공간 속에서의 시점 변화 및 인터랙션을 줄 수 있으며 가상공간은 사용자가 설정한 다양한 환경으로 변경이 가능하도록 하였다. 발표자는 가상공간 속에 별도로 제공되는 뷰에 표시된 프리젠테이션 파일 및 스크립트를 보며 내용 숙지 및 발표 숙달 연습을 하게 된다.

• 복식문화연구
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• 제31권5호
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• pp.627-639
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• 2023

This study seeks to increase the satisfaction of elderly men when purchasing and wearing ready-to-wear clothes by designing a slacks pattern suitable for their body type, which is determined by analyzing their lower bodies using virtual avatars and 3D virtual simulation system. The study found the following. First, based on virtual visualization of the comparison slacks pattern, the waistline position was consistently the lowest scored question among the evaluation survey items. Interpretation of this dissatisfaction suggests that, because the front waistline falls below the abdomen, the lower body, and especially the abdominal shape, is unpleasantly emphasized. Second, by using a virtual simulation system, the study developed a new slacks pattern that considered the concerns of elderly men. The primary measurement changes were as follows: front waist girth W/4+1.5cm+0.5cm, back waist girth W/4+1.5cm-0.5cm, front hip girth H/4+2.5cm-0.5cm, back hip girth H/4+2.5cm+0.5cm. Third, the new slacks pattern's appearance was evaluated more highly than the comparison pattern, confirming the new pattern's appropriateness for elderly men. This study demonstrates how slacks and other clothing patterns designed in a 3D virtual garment simulator can be used to design more appealing clothing for elderly men, increasing the satisfaction of wearing ready-made clothes at older ages.