• Journal of the Computational Structural Engineering Institute of Korea
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• v.29 no.5
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• pp.377-388
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• 2016

This study is aimed to evaluate the embedment effect of foundation as compared to the surface foundation on the response of a base-isolated nuclear power plant structure. For this purpose, the boundary reaction method (BRM), which is a two-step frequency domain and time domain technique, is used for the nonlinear SSI analysis considering nonlinear behavior of base isolators. The numerical model of the BRM is verified by comparing the numerical results obtained by the BRM and the conventional frequency-domain SSI analysis for an equivalent linear SSI system. Finally, the displacement response of the base isolation and the horizontal response of the structure obtained by the nonlinear SSI analysis using the moat foundation model are compared with those using the surface foundation model. The comparison showed that the displacement response of the base isolation can be reduced by considering the embedment effect of foundation.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.837-842
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• 2013

A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.