• Title/Summary/Keyword: 3D Structure Analysis

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Characterization of a cDNA Encoding Transmembrane Protein 258 from a Two-spotted Cricket Gryllus bimaculatus (쌍별귀뚜라미(Gryllus bimaculatus)의 GbTmem258 cDNA 클로닝과 발현분석)

  • Kisang Kwon;Honggeun Kim;Hyewon Park;O-Yu Kwon
    • Journal of Life Science
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    • v.33 no.10
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    • pp.828-834
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    • 2023
  • The cDNA that encodes transmembrane protein 258 (Tmem258) was cloned from Gryllus bimaculatus and named GbTmem258. This protein comprises 80 amino acids, has no N-glycosylation site, and contains five potential phosphorylation sites at two serines, two threonines, and one tyrosine. The predicted molecular mass of GbTmem258 is 9.06 kDa, and its theoretical isoelectric point is 5.5. The tertiary structure of GbTmem258 was predicted using the available secondary structure information, which suggests the presence of alpha helices (52.5%), random coils (22.5%), extended strands (16.25%), and beta turns (8.75%). Homology analysis revealed that GbTmem258 exhibits high similarity at the amino-acid level to Tmem258 found in other species. The effect of starvation and refeeding on GbTmem258 mRNA expression was also examined in this study. It was found that GbTmem258 mRNA expression in the hindgut progressively increased throughout the starvation period, peaking at almost 1.5 times the control level after six days of starvation. However, refeeding for one to two days after the six-day starvation period restored GbTmem258 mRNA expression to the control level. In fat body, GbTmem258 mRNA expression was almost 3-fold higher during starvation compared to the control level. Refeeding for one to two days after the six-day fast resulted in a decline in the expression to about a 2.5-fold increase over the control level. Throughout the starving and refeeding periods, no other tissues showed any discernible alterations in GbTmem258 mRNA expression.

Growth Environment and Vegetation Structure of Cephalotaxus koreana Nakai in South Korea Natural Habitats (국내 개비자나무 자생지 생육환경 및 식생구조)

  • Kim, Young Ki;Kim, Joon Seon;Lee, Kap Yeon;Kim, Moon Sup
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.384-395
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    • 2018
  • This study was carried out to investigate the environment factors including community structure and soil characteristics in the wild habitats of Cephalotaxus koreana, and offers the basic information for habitats conservation and restoration. Most of the wild habitats were located at altitudes between 148~835 m with inclinations ranged as $12{\sim}32^{\circ}$. The average soil pH was 4.7~5.9, soil organic matter was 5.72~15.99%, cation exchange capacity was $14.1{\sim}19.9cmolc/kg^{-1}$ and exchangeable $K^+$, $Ca^{2+}$, $Mg^{2+}$ was 0.25~0.48 cmolc/kg, 0.79~6.68 cmolc/kg, 0.31~1.73 cmolc/kg, respectively. The dominant species of tree layer were found to be dominated by Quercus dentata in Jekbo-san (C1), Acer pictum in Bogae-san (C2), Acer pseudosieboldianum in Geumwon-san (C3), Q. serrata in Jiri-san (C4), Zelkova serrata in Baegun-san (C5), and Q. acutissima in Duryun-san (C6). The Species diversity (H') was 0.854~1.234, evenness (J') was 0.654~0.993, and dominance (D) was found to be 0.067~0.346. Correlation coefficients analysis based on environmental factors, community structure and value of species diversity shows that growth of Cephalotaxus koreana is correlated with species diversity and evenness. This result show that Cephalotaxus koreana habitats located in mature stands.

A Destructive Method in the Connection of the Algorithm and Design in the Digital media - Centered on the Rapid Prototyping Systems of Product Design - (디지털미디어 환경(環境)에서 디자인 특성(特性)에 관한 연구(硏究) - 실내제품(室內製品) 디자인을 중심으로 -)

  • Kim Seok-Hwa
    • Journal of Science of Art and Design
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    • v.5
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    • pp.87-129
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    • 2003
  • The purpose of this thesis is to propose a new concept of design of the 21st century, on the basis of the study on the general signification of the structures and the signs of industrial product design, by examining the difference between modern and post-modern design, which is expected to lead the users to different design practice and interpretation of it. The starting point of this study is the different styles and patterns of 'Gestalt' in the post-modern design of the late 20th century from modern design - the factor of determination in industrial product design. That is to say, unlike functional and rational styles of modern product design, the late 20th century is based upon the pluralism characterized by complexity, synthetic and decorativeness. So far, most of the previous studies on design seem to have excluded visual aspects and usability, focused only on effective communication of design phenomena. These partial studies on design, blinded by phenomenal aspects, have resulted in failure to discover a principle of fundamental system. However, design varies according to the times; and the transformation of design is reflected in Design Pragnanz to constitute a new text of design. Therefore, it can be argued that Design Pragnanz serves as an essential factor under influence of the significance of text. In this thesis, therefore, I delve into analysis of the 20th century product design, in the light of Gestalt theory and Design Pragnanz, which have been functioning as the principle of the past design. For this study, I attempted to discover the fundamental elements in modern and post-modern designs, and to examine the formal structure of product design, the users' aesthetic preference and its semantics, from the integrative viewpoint. Also, with reference to history and theory of design my emphasis is more on fundamental visual phenomena than on structural analysis or process of visualization in product design, in order to examine the formal properties of modern and post-modern designs. Firstly, In Chapter 1, 'Issues and Background of the Study', I investigated the Gestalt theory and Design Pragnanz, on the premise of formal distinction between modern and post-modern designs. These theories are founded upon the discussion on visual perception of Gestalt in Germany in 1910's, in pursuit of the principle of perception centered around visual perception of human beings. In Chapter 2, I dealt with functionalism of modern design, as an advance preparation for the further study on the product design of the late 20th century. First of all, in Chapter 2-1, I examined the tendency of modern design focused on functionalism, which can be exemplified by the famous statement 'Form follows function'. Excluding all unessential elements in design - for example, decoration, this tendency has attained the position of the international style based on the spirit of Bauhause - universality and regularity - in search of geometric order, standardization and rationalization. In Chapter 2-2, I investigated the anthropological viewpoint that modern design started representing culture in a symbolic way including overall aspects of the society - politics, economics and ethics, and its criticism on functionalist design that aesthetic value is missing in exchange of excessive simplicity in style. Moreover, I examined the pluralist phenomena in post-modern design such as kitsch, eclecticism, reactionism, hi-tech and digital design, breaking away from functionalist purism of modern design. In Chapter 3, I analyzed Gestalt Pragnanz in design in a practical way, against the background of design trends. To begin with, I selected mass product design among those for the 20th century products as a target of analysis, highlighting representative styles in each category of the products. For this analysis, I adopted the theory of J. M Lehnhardt, who gradated in percentage the aesthetic and semantic levels of Pragnantz in design expression, and that of J. K. Grutter, who expressed it in a formula of M = O : C. I also employed eight units of dichotomies, according to the G. D. Birkhoff's aesthetic criteria, for the purpose of scientific classification of the degree of order and complexity in design; and I analyzed phenomenal aspects of design form represented in each unit. For Chapter 4, I executed a questionnaire about semiological phenomena of Design Pragnanz with 28 units of antonymous adjectives, based upon the research in the previous chapter. Then, I analyzed the process of signification of Design Pragnanz, founded on this research. Furthermore, the interpretation of the analysis served as an explanation to preference, through systematic analysis of Gestalt and Design Pragnanz in product design of the late 20th century. In Chapter 5, I determined the position of Design Pragnanz by integrating the analyses of Gestalt and Pragnanz in modern and post-modern designs In this process, 1 revealed the difference of each Design Pragnanz in formal respect, in order to suggest a vision of the future as a result, which will provide systemic and structural stimulation to current design.

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An Evaluation of Allowable Bearing Capacity of Weathered Rock by Large-Scale Plate-Bearing Test and Numerical Analysis (대형평판재하시험 및 수치해석에 의한 풍화암 허용지지력 평가)

  • Hong, Seung-Hyeun
    • Journal of the Korean Geotechnical Society
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    • v.38 no.10
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    • pp.61-74
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    • 2022
  • Considering that the number of cases in which a structure foundation is located on weathered rock has been increasing recently, for adequate design bearing capacity of a foundation on weathered rock, allowable bearing capacities of such foundations in geotechnical investigation reports were studied. With reference to the study results, the allowable bearing capacity of a foundation on weathered rock was approximately 400-700 kN/m2, with a large variation, and was considered a conservative value. Because the allowable bearing capacity of the foundation ground is an important index in determining the foundation type in the early design stage, it can have a significant influence on the construction cost and period according to the initial decision. Thus, in this study, six large-scale plate-bearing tests were conducted on weathered rock, and the bearing capacity and settlement characteristics were analyzed. According to the test results, the bearing capacities from the six tests exceeded 1,500 kN/m2, and it shows that the results are similar with the one of bearing capacity formula by Pressuremeter tests when compared with the various bearing capacity formula. In addition, the elastic modulus determined by the inverse calculation of the load-settlement behavior from the large-scale plate-bearing tests was appropriate for applying the elastic modulus of the Pressuremeter tests. With consideration of the large-scale plate-bearing tests in this study and other results of plate-bearing tests on weathered rock in Korea, the allowable bearing capacity of weathered rock is evaluated to be over 1,000 kN/m2. However, because the settlement of the foundation increases as the foundation size increases, the allowable bearing capacity should be restrained by the allowable settlement criteria of an upper structure. Therefore, in this study, the anticipated foundation settlements along the foundation size and the thickness of weathered rocks have been evaluated by numerical analysis, and the foundation size and ground conditions, with an allowable bearing capacity of over 1,000 kN/m2, have been proposed as a table. These findings are considered useful in determining the foundation type in the early foundation design.

Assessment of Response Spectrum by Dynamic Centrifuge Test for the Pile Foundation into the Clay (동적 원심모형실험에 의한 점성토 지반에 근입된 말뚝지지 기초의 응답 스펙트럼 분석)

  • Kim, Sang-Yeon;Park, Jong-Bae;Park, Yong-Boo;Kim, Dong-Soo
    • Land and Housing Review
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    • v.5 no.2
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    • pp.115-120
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    • 2014
  • Site coefficient and amplification factor of current domestic Seismic Design Code (KBC-2009) have no consideration for the domestic ground condition in which the base rock is normally placed within 30m form the surface. Accordingly, in this study dynamic centrifugal test and analysis for pile foundation into clay were achieved. and the response spectrums of free surface and basement were compared with each other. Within the period 1sec., the measured spectral acceleration of free surface and basement was bigger than the design spectral acceleration of SC and SD site. However the measured spectral acceleration of free surface and basement for the period over 1.5sec. was smaller than the design spectral acceleration of SC site. There was no severe difference of spectral acceleration according to the upper structure, embedded depth of foundation and free surface conditions. Consequently, normal domestic apartment housing for the period range over 1.5sec. could be design more economically applying these test result.

Isolation and Identification of Inhibitory Compound from Crataegi Fructus on ${\alpha}-amylase$ and ${\alpha}-glucosidase$ (산사(Crataegi Fructus) 추출물로부터 ${\alpha}-amylase$${\alpha}-glucosidase$ 저해 물질 분리 및 동정)

  • Kim, Myung-Uk;Cho, Young-Je;Kim, Jeung-Hoan
    • Applied Biological Chemistry
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    • v.50 no.3
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    • pp.204-209
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    • 2007
  • The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from Crataegi Fructus on ${\alpha}-amylase$ and ${\alpha}-glucosidase$. The content of total phenolic compounds of water extract from Crataegi Fructus was 22.5 mg/g. The inhibitory activity of the water extract (200 ${\mu}g/ml$) from Crataegi Fructus on ${\alpha}-amylase$ and ${\alpha}-glucosidase$ was determined to be 100% and 82.6%, respectively. Isolation of inhibitory compounds was carried out on Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure of increasing MeOH in $H_2O$. The chemical structure of the inhibitory compound against ${\alpha}-amylase$ and ${\alpha}-glucosidase$ was confirmed as chlorogenic acid by spectroscopic analysis of FAB-MS, NMR and IR spectrum.

The relationship between dental implant stability and trabecular bone structure using cone-beam computed tomography

  • Kang, Se-Ryong;Bok, Sung-Chul;Choi, Soon-Chul;Lee, Sam-Sun;Heo, Min-Suk;Huh, Kyung-Hoe;Kim, Tae-Il;Yi, Won-Jin
    • Journal of Periodontal and Implant Science
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    • v.46 no.2
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    • pp.116-127
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    • 2016
  • Purpose: The objective of this study was to investigate the relationships between primary implant stability as measured by impact response frequency and the structural parameters of trabecular bone using cone-beam computed tomography(CBCT), excluding the effect of cortical bone thickness. Methods: We measured the impact response of a dental implant placed into swine bone specimens composed of only trabecular bone without the cortical bone layer using an inductive sensor. The peak frequency of the impact response spectrum was determined as an implant stability criterion (SPF). The 3D microstructural parameters were calculated from CT images of the bone specimens obtained using both micro-CT and CBCT. Results: SPF had significant positive correlations with trabecular bone structural parameters (BV/TV, BV, BS, BSD, Tb.Th, Tb.N, FD, and BS/BV) (P<0.01) while SPF demonstrated significant negative correlations with other microstructural parameters (Tb.Sp, Tb.Pf, and SMI) using micro-CT and CBCT (P<0.01). Conclusions: There was an increase in implant stability prediction by combining BV/TV and SMI in the stepwise forward regression analysis. Bone with high volume density and low surface density shows high implant stability. Well-connected thick bone with small marrow spaces also shows high implant stability. The combination of bone density and architectural parameters measured using CBCT can predict the implant stability more accurately than the density alone in clinical diagnoses.

Clinical Study of the Floating-Sinking Pulse Quantification Analysis on Ages, Left/Right, and Palpation Positions (20/60대 여성을 중심으로 살펴본 좌우 촌관척 부/침맥 정량화 임상연구)

  • Kim, Jae-Uk;Kim, Sung-Hun;Jeon, Young-Ju;Ryu, Hyun-Hee;Lee, Yu-Jung;Lee, Hae-Jung;Kim, Jong-Yoel
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.5
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    • pp.1193-1198
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    • 2009
  • Pulse diagnosis is a central diagnosis method used in traditional Oriental medicine. To standardize and modernize the pulse diagnosis method, it is essential to develop an instrument-based reinterpretation of the clinically used pulse images in terms of the physical quantities such as the strength, period, width, length, and depth of the pulse. As a step towards such standardization, we conducted a clinical study on the floating/sinking pulses based on an automated palpation instrument (3D-MAC, Daeyo Medi, Korea) for 213 female subjects in their 20s and 174 female subjects in their 60s. The floating/sinking pulses are the two representative pulse images depending only on the depth of the pulse, and can be conveniently scaled by the coefficient of the floating-sinking pulse ($C_{fs}{\in}(0,1)$), which represents how strong one should apply the hold-down pressure to obtain the maximal pulse strength. As a result, primarily we found that it tends to appear more floating-like pulse ($C_{fs}{\rightarrow}0$) at Gwan and more sinking-like pulse ($C_{fs}{\rightarrow}1$) at Cheek, at both age groups and at both wrists. This result is consistent with a previous study on the geometrical structure of the blood vessel by an ultrasonograph. Second, the pulse tends to be more sinking-like in the age group of 60s than 20s. Finally, the pulses at the right palpation positions were found to be more sinking-like than the left, at both age groups.

The Road Map of Animation Festival in Korea through the Comparative Analysis of 4 International Animation Festivals (4대 국제애니메이션영화제 비교분석을 통한 한국애니메이션영화제 발전방향)

  • Choi, Young-Chul;Choi, Seung-Rak
    • Cartoon and Animation Studies
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    • s.25
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    • pp.177-201
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    • 2011
  • This article intends to draw the Road map of Korean International Animation Festival through the research of background and its identity of 4 International Animation Film Festivals Such as Annecy, Zagreb, Ottawa and Hiroshima. I was given opportunity to visit Annecy since 2009, it brought me to the real attraction of animation for watching of pleasure and passion of the people those who love animations. For the combining more advanced system and structure for Animation Festival in Korea, I had to do research all the information from the documents from the Annecy Collections. though I have not get the chance to go others except Annecy, However, I could get their background and history whenever I met the other Festival Committee Members. These Festivals showed us successful Road Map for the Animation Festivals in Korea as a role model. For the getting advanced system of Animation Festivals in Korea. It requires the animation theater for animation, effort for the Audiences's Convenience and international network composition system. However, the last task of us is to make people to entertain and enjoy the animation Films, its world of attraction.

Analysis of the Evacuation Safety with the Structure and Opening/Closing of Exits in a Fire at a Highway Service Area (고속도로 휴게소 화재 시 출입문의 구조와 개폐에 따른 피난안전성 분석)

  • Lee, Jai Young;Kong, Ha-Sung
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.2
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    • pp.419-425
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    • 2021
  • This study analyzed the safety of evacuation according to the number of exits and the direction of evacuation routes with evacuation simulations of the Pathfinder in case of a fire at a highway service area. It was also analyzed of the difference in RSET by comparing the single or double types of the exit of a facility. The results were as follows. When only one direction exit was opened, all of the RSET were exceeded. When two or more different directions were opened according to the general principle of evacuation, all results met the RSET. The simulations showed that two or more different directions were more shortened in RSET than the one same direction. The result of the types of doors showed that the single type door was shortened in RSET rather than the double type doors. For the evacuation safety in a fire at the highway service areas, firstly, it is necessary to secure two or more exits in a fire at all times. Secondly, the exits should not be same directions. Finally, it is suggested that the exit should be installed with a single type of door.