• Title/Summary/Keyword: 3D Library

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Cloning and Expression of the Gene Encoding Glucose Permease of the Phosphotransferase System from Brevibacterium flavum in Escherichia coli

  • Kwon, Il;Lee, Kyu-Nam;Lee, Jung-Kee;Pan, Jae-Gu;Oh, Tae-Kwang;Lee, Hyung-Hoan;Yoon, Ki-Hong
    • Journal of Microbiology and Biotechnology
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    • v.5 no.4
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    • pp.188-193
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    • 1995
  • A Brevibacterium flavum gene coding for glucose permease of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned by complementing the Escherichia coli ZSCl13 mutations affecting a ptsG gene with the B. flavum genomic library. From the E. coli clone grown as red colony on a MacConkey plate supplemented with glucose as an additional carbon source, a recombinant plasmid was isolated and named pBFT93. The plasmid pBFT93 was identified as carrying a 3.6-kb fragment of B. flavum chromosomal DNA which enables the E. coli transformant to use glucose or man nose as a sole carbon source in an M9 minimal medium. The non-metabolizable sugar analogues, 2-deoxy-D-glucose (2-DG) and methyl-$\alpha$-D-glucopyranoside (MeGlc) affected the growth of ZSCl13 cells carrying the plasmid pBFT93 on minimal medium supplemented with non-PTS carbohydrate, glycerol, as a sole cabon source, while the analogues did not repress the growth of ZSCl13 cells without pBFT93. It was also found that both $2-deoxy-D-[U-^{14}C]glucose{\;}and{\;}methyl-{\alpha}-D-[U-^{14}C]glucopyranoside$ could be effectively transported into ZSCl13 cells transformed with plasmid pBFT93. Several in vivo complementation studies suggested that the B. flavum DNA in pBFT93 encodes a glucose permease specific for glucose and mannose.

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Selection of New High-maintenance Children's Activity Spaces based on Children's Life Patterns (어린이 활동양상 설문분석을 통한 신규관리 활동공간 검토)

  • Kim, Ho-Hyun;Choi, In-Seak;Nam, Yi-Hyun;Lee, Jeong-Hun;Yoo, Si-Eun;Park, Choong-Hee;Lee, Jung-Sub
    • Journal of Environmental Health Sciences
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    • v.45 no.2
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    • pp.164-172
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    • 2019
  • Objectives: This study's purpose is finding children's activity spaces that demand environmental safety management. Methods: The method of this study is analysing children's life patterns based on a questionnaire survey. Results: This study analyzed children's life patterns through a questionnaire survey. In total, 2,447 questionnaires were provided to analyze children's life patterns. The results of the questionnaire indicated a highly simple form because many children generally stayed in their home (66%) or nursery facility (2%). In the case of other facilities, playground was ranked first and amusement park was ranked second. In addition, kids cafe (including play facilities installed in shopping centers, etc.), library, and internet cafe were among the responses. Conclusions: The priority for new high-maintenance children's activity spaces are academy (rank 1), kids cafe (rank 2), indoor playground (rank 3).

Studies on the High-gain Low Noise Amplifier for 60 GHz Wireless Local Area Network (60 GHz 무선 LAN의 응용을 위한 고이득 저잡음 증폭기에 관한 연구)

  • 조창식;안단;이성대;백태종;진진만;최석규;김삼동;이진구
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.41 no.11
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    • pp.21-27
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    • 2004
  • In this paper, millimeter-wave monolithic integrated circuit(MIMIC) low noise amplifier(LNA) for V-band, which is applicable to 60 GHz wireless local area network(WLAN), was fabricated using the high performance 0.1 ${\mu}{\textrm}{m}$ $\Gamma$-gate pseudomorphic high electron mobility transistor(PHEMT). The DC characteristics of PHEMT are drain saturation current density(Idss) of 450 mA/mm and maximum transconductance(gm, max) of 363.6 mS/mm. The RF characteristics were obtained the current gain cut-off frequency(fT) of 113 GHz and the maximum oscillation frequency(fmax) of 180 GHz. V-band MIMIC LNA was designed using active and passive device library, which is composed of 0.1 ${\mu}{\textrm}{m}$ $\Gamma$-gate PHEMT and coplanar waveguide(CPW) technology. The designed V-band MIMIC LNA was fabricated using integrated unit processes of active and passive device. The measured results of V-band MIMIC LNA are shown S21 gain of 21.3 dB, S11 of -10.6 dB at 60 GHz and S22 of -29.7 dB at 62.5 GHz. The measured result of V-band MIMIC LNA was shown noise figure (NF) of 4.23 dB at 60 GHz.

Gesture interface with 3D accelerometer for mobile users (모바일 사용자를 위한 3 차원 가속도기반 제스처 인터페이스)

  • Choe, Bong-Whan;Hong, Jin-Hyuk;Cho, Sung-Bae
    • 한국HCI학회:학술대회논문집
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    • 2009.02a
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    • pp.378-383
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    • 2009
  • In these days, many systems are equipped with people to infer their intention and provide the corresponding service. People always carry their own mobile device with various sensors, and the accelerator takes a role in this environment. The accelerator collects motion information, which is useful for the development of gesture-based user interfaces. Generally, it needs to develop an effective method for the mobile environment that supports relatively less computational capability since huge computation is required to recognize time-series patterns such as gestures. In this paper, we propose a 2-stage motion recognizer composed of low-level and high-level motions based on the motion library. The low-level motion recognizer uses the dynamic time warping with 3D acceleration data, and the high-level motion is defined linguistically with the low-level motions.

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A Variable-Length FFT/IFFT Processor for Multi-standard OFDM Systems (다중표준 OFDM 시스템용 가변길이 FFT/IFFT 프로세서)

  • Yeem, Chang-Wan;Shin, Kyung-Wook
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.35 no.2A
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    • pp.209-215
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    • 2010
  • This paper describes a design of variable-length FFT/IFFT processor (VL_FCore) for OFDM-based multi-standard communication systems. The VL_FCore adopts in-place single-memory architecture, and uses a hybrid structure of radix-4 and radix-2 DIF algorithms to accommodate various FFT lengths in the range of $N=64{\times}2^k\;(0{\leq}k{\leq}7)$. To achieve both memory size reduction and the improved SQNR, a two-step conditional scaling technique is devised, which conditionally scales the intermediate results of each computational stage. The performance analysis results show that the average SQNR's of 64~8,192-point FFT's are over 60-dB. The VL_FCore synthesized with a $0.35-{\mu}m$ CMOS cell library has 23,000 gates and 32 Kbytes memory, and it can operate with 75-MHz@3.3-V clock. The 64-point and 8,192-point FFT's can be computed in $2.25-{\mu}s$ and $762.7-{\mu}s$, respectively, thus it satisfies the specifications of various OFDM-based systems.

A variable-length FFT/IFFT processor design using single-memory architecture (단일메모리 구조의 가변길이 FFT/IFFT 프로세서 설계)

  • Yeem, Chang-Wan;Shin, Kyung-Wook
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2009.10a
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    • pp.393-396
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    • 2009
  • This paper describes a design of variable-length FFT/IFFT processor for OFDM-based communication systems. The designed FFT/IFFT processor adopts the in-place single-memory architecture, and uses a hybrid structure of radix-4 and radix-2 DIF algorithms to accommodate FFT lengths of $N=64{\times}2^k$ ($0{\leq}k{\leq}7$). To achieve both memory size reduction and the improved SQNR, a two-step conditional scaling technique is devised, which conditionally scales the intermediate results of each computational stage. The performance analysis results show that the average SQNR's of 64~8,192-point FFT's are over 60-dB. The processor synthesized with a $0.35-{\mu}m$ CMOS cell library can operate with 75-MHz@3.3-V clock, and 64-point and 8,192-point FFT's can be computed in $2.55-{\mu}s$ and $762.7-{\mu}s$, respectively, thus it satisfies the specifications of wireless LAN, DMB, and DVB systems.

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Research on Object Detection Library Utilizing Spatial Mapping Function Between Stream Data In 3D Data-Based Area (3D 데이터 기반 영역의 stream data간 공간 mapping 기능 활용 객체 검출 라이브러리에 대한 연구)

  • Gyeong-Hyu Seok;So-Haeng Lee
    • The Journal of the Korea institute of electronic communication sciences
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    • v.19 no.3
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    • pp.551-562
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    • 2024
  • This study relates to a method and device for extracting and tracking moving objects. In particular, objects are extracted using different images between adjacent images, and the location information of the extracted object is continuously transmitted to provide accurate location information of at least one moving object. It relates to a method and device for extracting and tracking moving objects based on tracking moving objects. People tracking, which started as an expression of the interaction between people and computers, is used in many application fields such as robot learning, object counting, and surveillance systems. In particular, in the field of security systems, cameras are used to recognize and track people to automatically detect illegal activities. The importance of developing a surveillance system, that can detect, is increasing day by day.

Effect of Ferrous Ion on the Formation of Exotoxin A from Pseudomonas sp. PY002 and Cloning of it's Gene (Pseudomonas sp. PY002에서 Exotoxin A의 생성에 미치는 철 이온의 영향과 Exotoxin A 유전자의 클로닝)

  • Choi, Sun-Ah;Kim, Ho-Sang;Choi, Ji-Young;Kang, Jeong-Suk;Kim, Chun-Sung;Kim, Duck-Lae;Kim, Young-Ju;Yeo, Myeong-Gu;Park, Yeol
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.7-12
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    • 1999
  • By SDS-polyacrylamide gel elcctrophoresis (SDS-PAGE) and immunoblot analysis that a protein with 66,000 daltons in size was recognized by P. aeruginosa anti-exotoxin A from P. sp. PY002. The yields of exotoxin A in P. sp. PY002 culture were influenced by the concentration of iron in the culture media. Increasing of the exotoxin A production and siderophore production was made slight increasing in the MKB medium. On the other hand, to clone the gene encoding the exoloxin A genomic library of P. sp. PY002 was constructed in pBluescript SK(+). From this library a exotoxin A homologous gene was isolated by immunological hybridization method using P. aemginosa anti-exoloxin A as a probe. Two putative clones were isolated and designated pETA23 and pETA42. Thc restriction analysis ol pETA42 demonstrated that thc 1760 bp insert contained one NcoI, PvuII, SstI, Kpnl and EcoRI site and three SmaI and HaeD sites.

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3D Casing-Distributor Analysis for Hydraulic Design Application

  • Devals, Christophe;Zhang, Ying;Dompierre, Julien;Vu, Thi C.;Mangani, Luca;Guibault, Francois
    • International Journal of Fluid Machinery and Systems
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    • v.8 no.3
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    • pp.142-154
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    • 2015
  • Nowadays, computational fluid dynamics is commonly used by design engineers to evaluate and compare losses in hydraulic components as it is less expensive and less time consuming than model tests. For that purpose, an automatic tool for casing and distributor analysis will be presented in this paper. An in-house mesh generator and a Reynolds Averaged Navier-Stokes equation solver using the standard $k-{\omega}$ shear stress transport (SST) turbulence model will be used to perform all computations. Two solvers based on the C++ OpenFOAM library will be used and compared to a commercial solver. The performance of the new fully coupled block solver developed by the University of Lucerne and Andritz will be compared to the standard 1.6ext segregated simpleFoam solver and to a commercial solver. In this study, relative comparisons of different geometries of casing and distributor will be performed. The present study is thus aimed at validating the block solver and the tool chain and providing design engineers with a faster and more reliable analysis tool that can be integrated into their design process.

Cloning of the Genomic DNA Which Complements the Drug-Hypersensitivity of Saccharomyces cerevlsiae

  • Lee, Yun-Sik;Park, Kie-In
    • BMB Reports
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    • v.30 no.3
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    • pp.167-172
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    • 1997
  • The yeast Saccharomyces cerevisiae, mutant CH117, shows a drug-hypersensitivity (dhs) to cycloheximide, bleomycin, actinomycin D, 5-fluorouracil. nystatin, nigericin and several other antibiotics. CH 117 was also temperature-sensitive (ts). being unable to grow at $37^{\circ}C$ and secreted more invertase and acid phosphatase into the medium than the parent yeast. CH117 grows very slowly and the cell shape is somewhat larger and more sensitive to zymolyase than the wild type cells. Light microscopic and electron microscopic observation also revealed abnormality of the mutant cell wall. These characteristics indicate that CH117 has a defect in an essential component of the cell surface and that the cell wall which performs barrier functions has become leaky in the mutant. We screened a genomic library of wild type yeast for clones that can complement the mutation of CH117. A plasmid, pCHX1, with an insert of 3.6 kilobases (kbs) could complement the dhs and ts of CH117. Deletion and subcloning of the 3.6 kb insert showed that a gene for the complementation of mutant phenotypes was located in 1.9 kbs Puvll-Hindlll fragment.

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