• Journal of Plant Biotechnology
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• v.7 no.3
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• pp.203-209
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• 2005

This study was conducted to improve tocopherol (vitamin E) composition in soybean (Glycine max) by introducing a gamma-tocopherol methyl transferase (${\gamma}$-TMT) gene via Agrobacterium tumefaciens-mediated transformation. Immature cotyledon explants were cocultivated with Agrobacterium tumefaciens. Putative transgenic embryos were selected from immature cotyledons on MS medium supplemented with 40 mg/L 2,4-D containing 100 mg/L kanamycin, 500 mg/L carbenicillin and 250 mg/L cefotaxime. Plantlets were developed from somatic embryos, and then transferred to soil. Nineteen regenerated plantlets obtained on the selection medium from 1,460 cotyledons. However, only 9 plantlets were confirmed as transformed plants. Integration of the transgene into the soybean genomic DNA was confirmed by PCR and Southern blot analysis. HPLC analysis showed that the content of ${\alpha}$-tocopherol in transgenic soybean seeds (AT-1) was approximately 4-fold higher than that of non-transgenic plants. Conclusively, we obtained the transgenic soybean having increased ${\alpha}$-tocopherol content by the overexpression of ${\gamma}$-TMT transgene.

• International Journal of High-Rise Buildings
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• v.6 no.2
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• pp.149-163
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• 2017

With the intensification of urban development in Chinese cities, mixed land use in urban centers extends vertically into 3-D and expands its scale from a single building to commercial clusters. The multi-level pedestrian system in city centers also changed its role from one of traffic isolation to spatial integration, where transit nodes, street sidewalks, squares, building entrances, atriums, and corridors are interconnected, both horizontally and vertically, into a whole spatial system, within which pedestrian flows are guided and shopping facilities are arranged. This paper uses spatial configuration analysis of space syntax to examine the impacts of spatial patterns on movement distribution and the business performance of tenant mix in the multi-level commercial system of the Nanshan Commercial Cultural District in Shenzhen, China. The key objective is to better understand the interactions between the socio-economic variables and spatial design parameters of a shopping complex. The research findings point to the importance of multiplicity between syntactic variables and other spatial variables in influencing the pedestrian flows, business performance and tenant mix in highly complex commercial systems. Particularly noteworthy is the relationship between spatial accessibility measures and the location of escalators, and the ways in which individual commercial buildings are embedded into the overall spatial system. The study suggests that this may lead to the preliminary identification of the spatial qualities of effective vertical extensions of mixed land use in a high-density urban settings.

• Journal of the Society of Naval Architects of Korea
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• v.51 no.5
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• pp.409-418
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• 2014

Simulation of flow past a complex marine structure requires a fine resolution in the vicinity of the structure, whereas a coarse resolution is enough far away from it. Therefore, a lot of grid cells may be wasted, when a simple Cartesian grid system is used for an Immersed Boundary Method (IBM). To alleviate this problems while maintaining the Cartesian frame work, we adopted an Adaptive Mesh Refinement (AMR) scheme where the grid system dynamically and locally refines as needed. In this study, We implemented a moving IBM and an AMR technique in our basic 3D incompressible Navier-Stokes solver. A Volume Of Fluid (VOF) method was used to effectively treat the free surface, and a recently developed Lagrangian Dynamic Subgrid-scale Model (LDSM) was incorporated in the code for accurate turbulence modeling. To capture vortex induced vibration accurately, the equation for the structure movement and the governing equations for fluid flow were solved at the same time implicitly. Also, We have developed an interface by using AutoLISP, which can properly distribute marker particles for IBM, compute the geometrical information of the object, and transfer it to the solver for the main simulation. To verify our numerical methodology, our results were compared with other authors' numerical and experimental results for the benchmark problems, revealing excellent agreement. Using the verified code, we investigated the following cases. (1) simulating flow around a floating sphere. (2) simulating flow past a marine structure.

• Journal of Korean Society for Geospatial Information Science
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• v.14 no.3 s.37
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• pp.47-53
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• 2006

In this contribution, an integration of seamless navigation system for the pedestrian is introduced. To overcome the GPS outages in various situations, multi-sensor of GPS, INS, electronic barometer and compass are considered in one Extented Kalman filter. Especially, the integrated system is designed for low-cost for the practical applications. Therefore, a MEMS IMU is considered, and the low quality of the heading is compensated by the electronic compass. In addition, only the pseudoranges from GPS measurements are considered for possible real-time application so that the degraded height is also controlled by a barometer. The mathematical models for each sensor with systematic errors such as biases, scale factors are described in detail and the results are presented in terms of a covariance analysis as well as the position and attitude errors compared to the high-grade GPS/INS combined solutions. The real application scenario of GPS outage is also investigated to assess the feasible accuracy with respect to the outage period. The description on the current status of the development and future research directions are also stated.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 1994.11a
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• pp.21-26
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• 1994

Conventional electroexplosive devices (EED) commonly use a very small metal bridgewire to ignite explosive materials i.e. pyrotechnics, primary and secondary explosives. The use of semiconductor devices to replace “hot-wire” resistance heating elements in automotive safety systems pyrotechnic devices has been under development for several years. In a typical 1 amp/1 watt electroexplosive devices, ignition takes place a few milliseconds after a current pulse of at least 25 mJ is applied to the bridgewire. In contrast, as for a SCB devices, ignition takes place in a few tens of microseconds and only require approximately one-tenth the input energy of a conventional electroexplosive devices. Typically, when SCB device is driven by a short (20 $\mu\textrm{s}$), low energy pulse (less than 5 mJ), the SCB produces a hot plasma that ignites explosive materials. The advantages and disadvantages of this technology are strongly dependent upon the particular technology selected. To date, three distinct technologies have evolved, each of which utilizes a hot, silicon plasma as the pyrotechnic initiation element. These technologies are 1.) Heavily doped silicon as the resistive heating initiation mechanism, 2.) Tungsten enhanced silicon which utilizes a chemically vapor deposited layer of tungsten as the initiation element, and 3.) a junction diode, fabricated with standard CMOS processes, which creates the initial thermal environment by avalanche breakdown of the diode. This paper describes the three technologies, discusses the advantages and disadvantages of each as they apply to electroexplosive devises, and recommends a methodology for selection of the best device for a particular system environment. The important parameters in this analysis are: All-Fire energy, All-Fire voltage, response time, ease of integration with other semiconductor devices, cost (overall system cost), and reliability. The potential for significant cost savings by integrating several safety functions into the initiator makes this technology worthy of attention by the safety system designer.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.205-206
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• 2017

Casual games are often have simple rule and easy to play. Like Angry birds, players can sling a bird as a bomb to destroy target. But the film adaption encounters numerous questions. games in pursuit of strong sensed presence, pay attention to experience; film stress the integrity of the story, attaches great importance to plot. However, casual games don't have story and plot. This paper take angry birds as example, analyzes the difficulties and method of adaptation from leisure mobile games to films, summarizes the key successful elements of adaption, such as subdivided the target group, chose an appropriate genre, write a script follow the basic logic and frame of film, set contradiction, complicated the character and still contain key elements of the game, in order to provide new ideas in the future development of integration of game and movie industry.

• Journal of Biomedical Engineering Research
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• v.32 no.2
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• pp.79-84
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• 2011

The purpose of this study was to investigate the relevance of the prediction equations derived from the relationship between metabolic energy expenditure and kinetic energy, for different speeds of walking and running over the treadmill. Seven male subjects participated in this study. A tri-axial accelerometer was attached on between the left and right posterior superior iliac spines. Kinetic energy was calculated by the integration of acceleration data and compared with the metabolic energy measured by a gas analyzer. Correlation coefficients were determined to find a relationship between the kinetic energy and the metabolic energy expenditure. Also, the difference between measured and predicted values was used to find the relevance for individual and group equations. Results showed a relatively good correlation between the measured metabolic energy and the calculated kinetic energy. In addition, a dramatic increase in kinetic energy was observed at the transition speed of walking and running (6 km/h). There was no difference in how to predict the kinetic energy expenditure for individual and group even though people have different physical characteristics. This study would be useful to predict metabolic energy expenditures by the regression analysis with acceleration data.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.34 no.1
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• pp.52-61
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• 1998

We studied behaviour pattern of anchovy (Engraulis japonicus) shoal by a method of shoal echo integration and tested species identification by a method of artificial neural network using the acoustic data collected in the East China Sea in March 1994 and in the southern coastal waters of the East Sea of Korea in March 1995. Between areas, frequency distribution of 10 shoal descriptors was different, which showed characteristics of shoal behaviour in size, bathymetric position and acoustic strength. The range and mean of shoal size distribution in length and height was wider and bigger in the southern coastal waters of the East Sea than in the East China Sea. Relative shoal size of China Sea. Fractal dimension of shoal was almost same in both areas. Mean volume reverbration index of shoal was 3 dB higher in the southern coastal waters of the East Sea than in the East China Sea. The depth layer of shoal distribution was related to bottom depth in the southern coastal waters of the East Sea, while it was between near surface and central layer in the East China Sea. Principal component analysis of shoal descriptors showed the correlation between shoal size and acoustic strength which was higher in the southern coastal waters of the East Sea, than in the East China Sea. Correlation was also found among the bathymetric positions of shoal to some degree higher in the southern coastal waters of the East Sea than in the East China Sea. The anchovy shoal of two areas was identified by artificial neural network. The contribution factor index (Cio) of the shoal descriptors between two areas were almost identical feature. The shoal volume reverberation index (Rv) was showed the highest contribution to the species identification, while shoal length and shoal height showed relatively high negative contribution to the species identification.

• Journal of Ocean Engineering and Technology
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• v.33 no.5
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• pp.400-410
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• 2019

In this study, the numerical code for the 3D nonlinear dynamic analysis of an SLWR (Steel Lazy Wave Riser) was developed using the lumped mass line model in a FORTRAN environment. Because the lumped mass line model is an explicit method, there is no matrix operation. Thus, the numerical algorithm is simple and fast. In the lumped mass line model, the equations of motion for the riser were derived by applying the various forces acting on each node of the line. The applied forces at the node of the riser consisted of the tension, shear force due to the bending moment, gravitational force, buoyancy force, riser/ground contact force, and hydrodynamic force based on the Morison equation. Time integration was carried out using a Runge-Kutta fourth-order method, which is known to be stable and accurate. To validate the accuracy of the developed numerical code, simulations using the commercial software OrcaFlex were carried out simultaneously and compared with the results of the developed numerical code. To understand the nonlinear dynamic characteristics of an SLWR, dynamic simulations of SLWRs excited at the hang-off point and of SLWRs in regular waves were carried out. From the results of these dynamic simulations, the displacements at the maximum bending moments at important points of the design, like the hang-off point, sagging point, hogging points, and touch-down point, were observed and analyzed.

• The Research Journal of the Costume Culture
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• v.29 no.1
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• pp.134-151
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• 2021

The aim of this research is to develop a clothing and textiles studio course for preservice home economics teachers applying principles of Project-Based Learning (PBL) and maker education to equip future teachers with the ability to nurture creativity among adolescents. The studio course was developed in the following stages: analysis, design, development, implementation, and evaluation. We concluded that the resulting course met the following objectives extracted from the 2015 revised curriculum of home economics subjects: to promote creative and environmentally-friendly fashion design and styling abilities, gain the ability to use makerspace tools, understand flat pattern making and sewing processes, and develop creative thinking, aesthetic sense, and communication skills. Furthermore, the educational effects of PBL and maker education were confirmed through student comments on the course. Students mentioned the practicality of the material in their actual lives along with their enhanced integration of the subject material, self-directedness, aesthetic sense, ability to learn through trial and error, collaboration and communication, and sharing. Based on results from the implementation and evaluation stages, a clothing and textiles studio course should include the following modules: introduction of terms and tools, submission and sharing of clothing reformation and upcycling techniques, introduction to hand sewing, pouch making, heat-transfer printing, 3D printing, mask making, hat making, vest making, and the final team project on fashion styling. It is important for instructors to provide detailed guidelines on selecting personas for styling, looking for available materials, and selecting materials online.