• Archives of Plastic Surgery
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• 제43권6호
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• pp.491-497
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• 2016

Background Hypertrophic scarring is a pathological condition that occurs after trauma or surgery. Angiogenesis occurs more often with hypertrophic scarring than with normotrophic scarring. The regulation of angiogenesis is one of the key factors in hypertrophic scar management. Vascular endothelial growth factor (VEGF) is an essential factor in the angiogenetic response. This study investigated whether decreasing the level of VEGF is effective for treating hypertrophic scarring. Methods Ten 8-week-old female New Zealand white rabbits were included. Four defects were created on each ear by using a 6-mm punch. Bevacizumab (Avastin, Roche Pharma, Basel, Switzerland) was administered in one ear and normal saline was administered in the other ear. Treatment was administered starting on day 2, every 2 days, until day 14. The levels of VEGF were measured using enzyme-linked immunosorbent assay on day 10 and histologic results were analyzed on day 40. Results Bevacizumab induced-defects showed less hypertrophic scarring when compared with the control group as measured by the scar elevation index (SEI) and loose collagen arrangement. The SEI in the experimental group was $1.89{\pm}0.13$, compared to $1.99{\pm}0.13$ in the control group (n=30, P=0.005). Additionally, the VEGF level was lower ($38.72{\pm}11.03pg$ vs. $82.50{\pm}21.64pg$, n=10, P=0.001) and fewer vessels existed ($8.58{\pm}0.76$ vs. $7.2{\pm}1.20$, n=10, P=0.007). Conclusions Preventing excessive angiogenesis is effective for preventing scar formation, especially with hypertrophic scarring. Although it is not an approach that is sufficient alone for the management of scarring, it may be one of several important strategies for scar treatment.

• 대한의생명과학회지
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• 제20권4호
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• pp.209-220
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• 2014

Vascular endothelial growth factor (VEGF) is a key factor involved in the induction of angiogenesis and has become an attractive target for anti-angiogenesis therapies. The purpose of this study was to elucidate the anti-angiogenic activity of Rubus coreanus Miquel water extract (RCME). Rubus coreanus Miquel has long been employed as a traditional medicine, and recent studies have demonstrated that it has measureable biological activities. Thus, we investigated for the first time the effect of RCME on angiogenesis and its underlying signaling pathways. The effects of RCME were tested on in vitro models of angiogenesis, namely, proliferation, migration, invasion and tube formation of human umbilical vein endothelial cells as well as an ex vivo model of vessel sprouting from the rat aorta in response to VEGF. We observed that VEGF-induced angiogenesis was strongly suppressed by RCME treatment compared to that of the control group. Moreover, we found that RCME inhibited VEGF-induced activation of matrix metalloproteinases and phosphorylation of extracellular signal-regulated kinase and p38, and also effectively inhibited phosphorylation of VEGF receptor 2. These results indicated that RCME inhibits angiogenesis by suppressing phosphorylation of the VEGF receptor and may be useful for the treatment of angiogenesis-dependent diseases such as cancer and diabetic retinopathy.

• 생명과학회지
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• 제17권8호통권88호
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• pp.1100-1103
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• 2007

합성항산화제 3종과 17종의 식물 추출물을 이용하여 양성자 빔을 1,000, 5,000, 10,000 Gray 수준으로 처리하여 에너지양에 따른 항산화 활성에 미치는 영향을 탐색 하고자 DPPH radical 소거능 과 ABTS cation radical 소거능의 활성에 대하여 조사하였다. 이중 6종류의 시료는 양성자 빔을 조사한 결과 활성이 감소하였으며, 2종류의 시료는 활성변화가 없었으며, 12종류의 시료는 활성이 증가하였다. DPPH radical 소거 효과는 1,000 Gray 에서 마황은 60%, 괄루인은 5,000 Gray 에서 77.8%로 활성이 증가하는 경향을 나타내었다. ABTS cation radical 소거효과에서 토천궁은 1,000 Gray의 양성자 빔을 조사한 결과 38.5% 활성이 감소하였으며, 합성 항산화제는 활성변화가 거의 없으나, 뼝쑥 추출물은 5,000 Gray에서 $IC_{50}$값이 2.4 ${\mu}g/ml$로 BHT의 $IC_{50}$값 2.3 ${\mu}g/ml$과 유사하였다.

• 한국미생물·생명공학회지
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• 제38권1호
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• pp.7-12
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• 2010

S. cerevisias의 치명적인 약점인 xylose 또는 arabinose가 상당부분을 차지하는 hemicellulose 기수분해물인 오탄당 발효의 극히 낮은 효율은 유전자 변형 및 대사적 흐름을 조절하여 세포 내로의 오탄당 섭취 및 활용 증가를 위한 연구가 꾸준히 진행되고 있다. S. cerevisie에서 오탄당은 육탄당보다 1-2 배 낮은 친화력을 가지고 있어, 오탄당 운반은 이를 이용한 바이오에탄올 발효에 있어서 중요한 초기 조절 단계이다. 오탄당 이용가능 S. cereivsiae에서 오탄당 운반기의 발현 관련 소수의 연구가 보고되고는 있으나 아직까지 눈에 띠는 효율 증기눈 보교되지 않았다. 최근 보고된 S. cerevisiae에서 C. intermeda 유래의 glucose/xylose의 확산을 용이하게 하는 운반기와 공동수송기의 이종발현이 처음으로 활성화 되었음이 보고 되었다. 따라서 그러므로 높은 친화력의 xylose 운반기의 발현은 이미 xylose로 부터 바이오에탄올 발효공정은 최적화되어 있지만 여전히 몇 가지 제한 요소들을 가지고 있는 S. cerevisiae 균주들의 xylsoe 발효공정 효율 향상에 큰 기여를 할 수 있을 것으로 기대된다.

• 산경연구논집
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• 제9권11호
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• pp.25-38
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• 2018

Purpose - This study aimed to identify the sub-dimensions of fashion social media quality (information quality, social quality, service quality, system quality) and investigate how they affect purchase intention through fashion information use behavior (information acceptance, information diffusion). Research design, data, and methodology - Data collection was carried out twice for systematic verification of the research model. In the first data collection, the reliability and validity of research variables were verified through 238 respondents and questionnaires were revised and supplemented based on their responses. In March 2018, the final survey was conducted from 755 respondents the age of 20 to 49. Using SPSS 23.0, descriptive statistics, exploratory factor analysis, correlation analysis were performed. In order to test hypotheses, structural equational modeling technique was employed using AMOS 23.0. Results - First of all, fashion Social media quality consists of four factors including information quality, social quality, service quality and system quality. Second, fashion Social media information quality, social quality, and system quality were shown to have a positive(+) effect on information acceptance behavior, and social quality, service quality and system quality were shown to have a positive(+) effect on information diffusion behavior. It was also determined that the acceptance and diffusion behaviors of fashion information through fashion Social media had positive(+) influence on purchase intention. Conclusions - This study holds academic significance in its identification of the components of fashion Social media quality and for conducting an empirical analysis on the causal relationship between fashion information acceptance and diffusion behaviors, and purchase intention. The results of this study indicate that fashion involvement is the key factors in determining the quality of Social media, the acceptance of information through Social media, and, by extension, the purchase of fashion products. Practitioners in the fashion industry may use the findings of this study in order to build more effective Social media strategy.

• 농촌계획
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• 제25권1호
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• pp.21-38
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• 2019

Korean agricultural industry has weakened as demand for domestic agricultural products has declined due to accelerating market liberalization, aging and shrinking of rural population, and stagnating rural households' incomes. On the other hand, as the forth industrial revolution unfolds in earnest, tremendous changes are expected, and those changes won't be confined to certain industries but would shaken the world we know of entirely. Smart farm, which is one example of the fourth industrial revolution, is increasingly being recognized as a new growth engine for the future as smart farm and the science and technology behind it, not the size of arable land, will determine competitiveness of the agricultural industry and drive agricultural productivity and managerial efficiency. In consideration that John W. Kingdon's Multiple Streams Framework has recently been presented as an important theoretical model in the policy field, this study analyzed problem stream, policy stream, and political stream in the process of forming the smart farm policy, and looked into what role the government played as policy entrepreneur in policy window. The smart farm policy was put on policy agenda by the government and was approved when the government announced the Smart Farm Plan together with relevant ministries at the 5th Economy-Related Ministers' Meeting held in April 2018. This suggests that change of the government is the most critical factor in political stream, and explicitly indicates the importance of politics in formation of an agricultural policy. In addition, actual outcome of the policy and how policy alternatives that will enhance people's understanding will support it seem to be the key to success. It also shows that it is important that policy alternatives be determined based on sufficient discussion amongst stakeholders.

• 비교문화연구
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• 제38권
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• pp.245-264
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• 2015

In this paper, I suggest methodological ways of studying comparative literature regarding ongoing discussions of world and national literature. The role of comparative literature studies has widened in the contemporary era, in which nations have become rapidly entangled and the concept of the world as a unified entity is under question. In this regard, I critically review the traditional principles of the hospitality of cosmopolitanism and the exclusivity of the borders of national literatures. Further, I suggest that scholars adopt the concept by Sigmund Freud of "unfamiliar familiarity" as a methodological motive for studies of comparative literature. Based on this concept, scholars can further develop the unique methods of the discipline of comparative literary studies for teaching and research amidst the ongoing phenomenon of globalization. They can also use these methods to simultaneously contribute to solving the problem of "comparison without a unifying category of the world," as revealed by the results of deconstructional and postcolonial studies. Regarding community-based discussions of literature, I introduce the "bridge and door" metaphor, put forth by Georg Simmel, as a key concept in methodological consideration of translation and in comparative literary studies. In this paper, adopting the metaphor of the bridge and door as an intertextual and social model for comparative studies, I define the new role of comparative literary studies in literary transnationalism, which is particularly necessary when different languages and cultures overlap and become entangled. Regarding the rapidly changing contemporary world community, comparative literary studies, as an experimental discipline, is uniquely capable of examining this kind of community, which forms itself beyond and beneath individual nations.

• Korean Journal of Radiology
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• 제22권3호
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• pp.425-434
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• 2021

Objective: To investigate the potential value of ¹⁸F-fluorodeoxyglucose (FDG) PET/CT in predicting the survival of patients with primary tracheal malignant tumors. Materials and Methods: An analysis of FDG PET/CT findings in 37 primary tracheal malignant tumor patients with a median follow-up period of 43.2 months (range, 10.8-143.2 months) was performed. Cox proportional hazards regression analyses were used to assess the associations between quantitative ¹⁸F-FDG PET/CT parameters, other clinic-pathological factors, and overall survival (OS). A risk prognosis model was established according to the independent prognostic factors identified on multivariate analysis. A survival curve determined by the Kaplan-Meier method was used to assess whether the prognosis prediction model could effectively stratify patients with different risks factors. Results: The median survival time of the 37 patients with tracheal tumors was 38.0 months, with a 95% confidence interval of 10.8 to 65.2 months. The 3-year, 5-year and 10-year survival rate were 54.1%, 43.2%, and 16.2%, respectively. The metabolic tumor volume (MTV), total lesion glycolysis (TLG), maximum standardized uptake value, age, pathological type, extension categories, and lymph node stage were included in multivariate analyses. Multivariate analysis showed MTV (p = 0.011), TLG (p = 0.020), pathological type (p = 0.037), and extension categories (p = 0.038) were independent prognostic factors for OS. Additionally, assessment of the survival curve using the Kaplan-Meier method showed that our prognosis prediction model can effectively stratify patients with different risks factors (p < 0.001). Conclusion: This study shows that ¹⁸F-FDG PET/CT can predict the survival of patients with primary tracheal malignant tumors. Patients with an MTV > 5.19, a TLG > 16.94 on PET/CT scans, squamous cell carcinoma, and non-E1 were more likely to have a reduced OS.

• 생명과학회지
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• 제27권1호
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• pp.38-43
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• 2017

강한 항 염증 활성을 갖는 glycosyl aesculin이 Thermotoga neapolitana ${\beta}-glucosidase$의 당전이 활성을 통하여 aesculin을 수용체로 이용하여 합성되었다. 약 $2{\mu}g$의 효소를 이용하여 반응표면분석법을 통한 주요 반응 매개변수들의 최적화가 시도되었다. 각 반응 변수들의 통계분석 결과 2차 다항식모델이 적용된 유의값(p<0.05)에 잘 맞았다. Aesculin과 다른 매개변수사이의 상호관계를 의미하는 반응표면곡선 그래프는 glycosyl aesculin의 수율이 주로 aesculin의 농도와 반응시간에 영향을 받음을 나타내었다. Glycosyl aesculin의 생산을 위한 반응최적조건은 aesculin의 농도 9.5 g/l, 온도 $84^{\circ}C$, 반응시간 81분, 그리고 pH 8.2로 나타났으며, 이러한 조건하에서 효소반응시 61.7%의 전환율로 5.86 g/l 의 수율이 예상되었다. 실험을 통한 실질적인 수율은 6.02 g/l으로 나타났다. 실질적인 수율과 가까운 값을 예측 가능하다는 것을 통하여 반응표면분석법이 효소반응의 전환율을 최적화하는데 타당하다는 것이 입증되었다. 본 연구에서는 반응표면분석법을 활용하여 최적화 이전에 비하여 약 1.6배의 glycosyl aesculin을 얻을 수 있었다. 이러한 결과들은 반응표면분석법이 미생물유래 당화효소를 이용한 생물학적 활성을 갖는 배당체 합성의 생산 최적화에 효과적으로 활용할 수 있다는 것을 보여준다.

• 대한본초학회지
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• 제38권5호
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• pp.9-19
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• 2023

Objectives : Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density and increased risk of fractures. Bisphosphonates and selective estrogen receptors, which are bone resorption inhibitors that are currently widely used as osteoporosis treatments, show serious side effects when administered for a long time. Research on bone resorption inhibitors that complement the problems of existing treatments is needed. The purpose of this study was to investigate the effect of inhibiting osteoclast differentiation and activity on the tuberous root of Ampelopsis japonica (Thunb.) Makino (AM). Methods : After extracting AM using distilled water and ethanol, the inhibitory effects of the two solvents on osteoclast differentiation were compared using the RANKL-induced in vitro experimental model and the TRAP assay kit. The impact of AM on bone resorption was investigated through the pit formation assay, and its effect on F-actin formation was assessed through fluorescent staining. Additionally, protein and mRNA expression levels of osteoclast differentiation markers (NFATc1, c-Fos, TRAP and ATP6v0d2) and resorption markers (MMP-9, CTK, and CA2) were analyzed via western blot and RT-PCR. Results : AM treatment significantly decreased the number of TRAP-positive cells and pit formation area. Furthermore, AM suppressed both the protein and mRNA expression of NFATc1 and c-Fos, key transcription factors involved in osteoclast differentiation and it downregulated the expression of osteoclast-associated genes such as TRAP, CTK, MMP-9, CA2, and ATP6v0d2. Conclusions : These results suggest that AM can inhibit bone resorption and osteoclast differentiation, indicating its potential for use in the treatment and prevention of osteoporosis.