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Application of 'Sponge Model' with Disinfectants for the Inhibition of Listeria monocytogenes (Listeria monocytogenes의 증식억제를 위한 살균제 'Sponge model'의 응용)

  • LEE Myung-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.5
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    • pp.595-602
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    • 1996
  • The antimicrobial effects of two disinfectants commonly used in food industry on Listeria monocytogenes ATCC 15313 were studied. The two disinfectants tested were commercial benzalkonium chloride (BAC) and sodium hypochlorite (NaOCl). Their effects were studied on cells suspended in disinfectants (in vitro) and in the sponge model with the disinfectants (in vivo). When cells were exposed to $0\~0.1\%$ BAC and $0\~150\;ppm$ NaOCL for 20 minutes, BAC and NaOCl concentration more than $0.25\%$ and 100 ppm showed the antimicrobial effects respectively. This organism decreased rapidly during the first $0.5\~1$ minute followed by a slower decrease during the rest of the exposure time. Fifteen ml of cell solution $(about\;10^7\;CFU/ml\;in\;the\;TSB)$ was mixed with 15 ml of disinfectants in the sponge $(6.0{\times}4.0{\times}4.0cm)$, BAC and NaOCl concentration more than $0.1\%$ and 300 ppm showed the antimicrobial effects, and at $0.25\%$ and 800 ppm diminished in cell numbers 3-log cycles during the first 20 minutes. In the case of sponge model, 15 ml of cell solution and 15 ml of disinfectants $(0.25\%\;of\;BAC,\;800\;ppm\;of\;NaOCl)$ were suspended in the sponge during 20 minutes, washing with 200 ml of sterilized distilled water, and this sponge was transfered in the 100 ml TBS, and then incubated at various temperature. The cells were increased about 1-log cycle during 24 hrs at $5\~15^{\circ}C$. And the others temperature, the cells growth was in proporation to storage tepmerature and the cells were about $10^9\;CFU/ml$ after $1\~3$ days incubations.

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Clinical Characteristics of Neonatal Status Epilepticus (신생아 간질 중첩증의 임상 특성)

  • Jung, Kyeong Hun;Kim, Yun Hee;Kwon, Young Se;Jun, Yong Hoon;Kim, Soon Ki;Son, Byong Kwan
    • Clinical and Experimental Pediatrics
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    • v.48 no.12
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    • pp.1342-1347
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    • 2005
  • Purpose : Among perinatal risk factors, neonatal seizures are one of the strongest independent discriminators of adverse outcome, representing high risks of mortality and neurologic morbidity. This study was undertaken to evaluate the neurologic outcome of neonatal status epilepticus according to underlying etiology, seizure pattern, onset time, and duration. Methods : We reviewed retrospectively 36 neonates(19 males, 17 females) with status epilepticus who were admitted to the neonatal intensive care unit, Inha Hospital between July, 1988 and June, 2003. They were evaluated with neurologic examination, laboratory data, EEG findings, and neuroimaging studies etc. Results : The mean gestational period of the patients was $37.0{\pm}3.6$ weeks and birth weight was $2.70{\pm}0.82$ kilogram. Fifty two point eight percent of the neonates were male and 66.7 percent were born at term. The most common cause of neonatal status epilepticus was hypoxic-ischemic encephalopathy. In preterm babies, intracranial hemorrhages showed an especially high frequency(P=0.034). Gestational age and birth weight did not show a correlation with neurologic complications. The incidence of neurological sequelae were significantly related to prolonged seizures lasting more than 1 hour(P=0.002). Neonates with seizures within the first 72 hours tended to be more frequent among those who developed adverse outcomes(P=0.016). Generalized tonic seizures had the worst prognosis, whereas those children who had subtle seizures had better outcomes than any other type(P<0.05). Generalized tonic seizures were primarily represented on EEG by abnormal background, whereas subtle seizure showed a significantly more normal EEG than any other seizures(P<0.05). Conclusion : Our results indicate that neonatal status epilepticus with early onsets, prolonged durations. And generalized tonic types can predict an increased risk for neurologic sequelae. So, those seizures must be perceived as medical emergencies and treated aggressively with antiepileptic drugs.

Relationship among Body Composition, Biochemical Measurements and Serum Leptin Level in Obese Children (비만아의 체성분 및 생화학적 측정치와 혈중 Leptin 농도와의 관계)

  • 최봉순;손수진;이희자;박명희;이은주
    • Journal of Nutrition and Health
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    • v.35 no.4
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    • pp.454-463
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    • 2002
  • The purpose of this study was to investigate the relationship among the anthropometric measurements, body composition indices, biochemical indices and serum leptin level of obese school children. The study was conducted on 97 elementary school children from 4-5th grade residing in Daegu and Kyungbuk area. Obesity was defined as fat percentage that exceed 25% of body fat mass. The prevalence of obesity of subject was 58.3% for male and 54.1% for female. The mean value of height, weight, body composition indices and biochemical indices of obese group were significantly higher than those of control group. The leptin level was significantly different in obese group but showed no difference by gender. The differences of leptin level were according to adiposity and BMI in obese subjects. Multiple regression analysis data showed that the BMI and body fat mass may influence on the leptin level of subject. Also, these data revealed the leptin resistance in obese children and relationship between leptin level and various factors including anthropometric measurements, body composition and biochemical indices. Additional research should be necessary to assess the mechanism of leptin resistance in obese children.

A Study of Food Attitudes at the Community Level (지역집단(地域集團)의 식품태도(食品態度)에 관(關)한 연구(硏究))

  • Kim, In-Sun;Tchai, Bum-Suk
    • Journal of Nutrition and Health
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    • v.9 no.1
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    • pp.76-83
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    • 1976
  • The aims of this study are, 1) to describe the attitudes of the community towards each food item and 2) to provide the information necessary for the solution of nutritional problems due to the different food intake of the community. This study is based on the Food Consumption Survey conducted by the Ministry of Health and Social Affairs in a series of National Nutrition Survey in August, 1973. The sample included 8 communities, in which 160 households were selected at random for this study. The procedures and methods used in this study to compute the acceptability index were adopted from the study of H. Toyokawa, University of Tokyo. Food attitudes of the sample communities are clear in terms of the mean acceptability index which is presented in Table 3. The following order is indicative of the relative strength of acceptability of the respective food items: 1. Seasonings (-1.20) 2. Oils and fats (-1.12) 3. Pulses(- .60) 4. Processed vegetables (- .45) 5. Seaweeds (- .32) 6. Sugars (- .19) 7. Fresh vegetables (- .15) 8. Rice (- .13) 9. Cereals and grain products (- .07) 10. Starchy roots (- .06) 11. Fresh fish and shells (.44) 12. Processed fish and shells (.56) 13. Eggs (1.06) 14. Fruits (1.26) 15. Meats and poultry (1.33) 16. Milk and milk products (2.87) There are highly significant difference in the acceptability index between food items and the mean intake of rice, cereals and grain products, pulses, starchy roots, processed vegetables, fruits, sugars, seaweeds, seasonings, meats and poultry, eggs, fresh and shells, and oils and fats between the communities at the .01 level of significance.

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A Study on the Nutritional Status in Hospitalized Patients with Pulmonary Tuberculosis (폐결핵 환자의 입원기간동안 영양상태 변화 연구)

  • You, Hyun-Jai;Kim, Yang-Ha
    • Journal of Nutrition and Health
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    • v.42 no.7
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    • pp.615-621
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    • 2009
  • This study was conducted to evaluate the change of nutritional status and to analyze related factors in hospitalized tuberculosis (TB) patients during their hospitalization. The subjects were 398 men patients (mean age: 47.3 ${\pm}$ 14.4 y) who had hospitalized more than 3 months at TB hospital located in Seoul. The anthropometric and blood biochemical indices were measured, and dietary intakes were assessed. At the time of admission the body weight of subjects was about 76% of the average body weight of Korean men with same age, Body mass index (BMI) of subjects was 18.5 kg/$m^2$, and 53.8% of subjects were under weight status. Average level of blood hemoglobin and hematocrit of subjects was lower than the normal value. After 3 months of hospitalization period, the body weight and body mass index were significantly increased compared to admission by 3.9 kg (7.41%) and 1.4 kg/$m^2$ (7.61%)(p < 0.001), respectively. Blood levels of hemoglobin, hematocrit, albumin, and total protein were also significantly increased after 3 months of hospitalization period compared to admission (p < 0.001). The increment in the body weight and blood indices was significantly higher in below 29 years group than over 60 years group (p < 0.05). The increment in the body weight and body mass index was significantly higher in the under-body weight group compared to the normal-body weight group (p < 0.05). In conclusion the body weight and body mass index of subjects were significantly increased after 3 months of hospitalization period, and the age and body weight of subjects at admission were supposed to influence the degree of change in the nutritional status.

Effects of Artificial Acid Rain on Seed Germination, Radicle Growth and Seedling Growth of Several Woody Species (인공산성우(人工酸性雨)가 몇 수종(樹種)의 종자발아(種子發芽), 유근생장(幼根生長) 및 묘목생장(苗木生長)에 미치는 영향(影響))

  • Kim, Gab-Tae
    • Journal of Korean Society of Forest Science
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    • v.81 no.1
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    • pp.30-39
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    • 1992
  • Artificial acid rain(pH2.0, 3.0, 4.0 and 5.0) was treated on the seeded pots of 4 species to examine its effects on germination, leaf injury and seedling growth. Aluminum ammonium sulfate solution (0.5, 1.0 and 2.5mM) and artificial acid rain (pH3.0, 4.0 and 5.0) were treated on the seeded germinators of 3 species to examine its effects on germination and radicle growth. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water(pH6.5) was used as control. Artificial acid rain was sprayed to seeded pots three times per week. About 5mm of artificial acid rain was treated each time from early April to early October, 1991. Germination, seedling establishment, radicle growth, leaf injury and seedling growth were measured and compared among the treatments. The results were summarized as follows 1. Germination, seedling establishment rate of Pinus densiflora were highest on the pH5.0 pot whereas those of Ailanthus altissima was highest on the control pot, and those of Magnolia sieboldii showed the highest value on the pH3.0 pot. 2. The differences in seedling height of Pinus densiflora and Pinus rigida were significant among the treatment levels of acid rain, whereas those of Magnolia sieboldii and Ailanthus altissima were not significant. Seedling heights of Pinus densiflora and Pinus rigida were highest on the pH3.0 pot, and that of Magnolia sieboldii was highest on the pH4.0 pot, and that of Ailanthus altissima was highest on the control pot. 3. Seedling dry weights of Pinus densiflora. Pinus rigida, and Ailanthus altissima differed significantly among the treatments, but that of Magnolia sieboldii did not differ. Highest seedling dry weights of Magnolia sieboldii and Ailanthus altissima were observed on the pH4.0 pot, and that of Pinus densiflora was observed on the pH3.0 pot, and that of Pinus rigida was observed on the pH5.0 pot. 4. With decrease of pH value of artificial acid rain, the number of injured needle and injured individual tended to be increased. 5. The differences in effects of soil acidity were highly significant among the treatments for all species. 6. Radicle growth of Pinus densiflora differed significantly among pH levels and aluminum concentrations.

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Effect of Microbial Phytase on Performance, Nutrient Absorption and Excretion in Weaned Pigs and Apparent Ileal Nutrient Digestibility in Growing Pigs

  • Zeng, Z.K.;Piao, X.S.;Wang, D.;Li, P.F.;Xue, L.F.;Salmon, Lorraine;Zhang, H.Y.;Han, X.;Liu, L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.8
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    • pp.1164-1172
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    • 2011
  • Two experiments were conducted to evaluate the efficacy of Trichoderma reesei derived phytase for pigs fed diets with fixed calcium to total phosphorus ratios (1.5:1). In Exp. 1, 280 weaned pigs (initial BW of $10.32{\pm}1.94$ kg) were allocated to one of five dietary treatments on the basis of weight and gender in a randomized complete block design. Treatments were the low phosphorus (0.6% Ca, 0.4% total P and 0.23% available P) diets supplemented with 0, 250, 1,000, or 2,000 FTU phytase/kg of diet and a positive control diet (PC; 0.85% Ca, 0.58% total P and 0.37% available P). The treatments were applied to seven pens with eight pigs per pen, half male and half female. In Exp. 2, six barrows fitted with ileal T-cannula (initial BW = $35.1{\pm}1.6$ kg) were assigned to three dietary treatments with a double $3{\times}3$ Latin square design. The dietary treatments were the low-phosphorus diet (0.53% Ca, 0.34% total P and 0.14% available P), the low phosphorus diet plus 1,000 FTU phytase/kg and a positive control diet (0.77% Ca, 0.50% total P and 0.30% available P). In Exp. 1, there were linear increases (p<0.01) in weight gain, phosphorus absorption, bone strength, calcium and phosphorus content of fat-free dried bone and plasma phosphorus concentrations with increasing dose rate of phytase. The performance of pigs fed the diets with 250, 1,000, or 2,000 FTU of phytase/kg did not differ from pigs fed the PC diet. Pigs fed diets with 1,000 or 2,000 FTU of phytase/kg did not differ from pigs fed the PC diet in bone characteristics. The apparent digestibility of dry matter, crude protein, ash and energy was not affected by dietary treatment. However, pigs fed the PC diet excreted more fecal phosphorus (g/d, p<0.01) and fecal phosphorus per BW gain (g/kg) than pigs fed the diets with phytase. Phytase linearly decreased (p<0.01) fecal phosphorus excreted per BW gain (g/kg), plasma calcium concentration as well as plasma and bone alkaline phosphatase activity. In Exp. 2, phytase supplementation in the low-P diet increased (p<0.05) the apparent ileal digestibility (AID) of Ca, P, leucine, lysine, phenylalanine, alanine and cysteine, tended to AID of crude protein, isoleucine, threonine, asparagine and serine. In conclusion, the novel phytase originated from Trichoderma reesei is effective in releasing Ca, P, and amino acids from corn soy based diet for pigs.

Surgical Approaches to the Middle Cranial Base Tumors (중두개와저 종양에 대한 수술적 치료)

  • Kim, Il Seub;Rha, Hyung Kyun;Lee, Kyung Jin;Cho, Kyung Keun;Park, Sung Chan;Park, Hae Kwan;Cho, Jeung Ki;Kang, Jun Ki;Choi, Chang Rhack
    • Journal of Korean Neurosurgical Society
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    • v.30 no.9
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    • pp.1079-1085
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    • 2001
  • Objective : We analysed various surgical approaches and surgical results of 28 middle cranial base tumors for the purpose of selecting optimal surgical approach to the middle cranial base tumor. Methods : In this retrospective review, 28 patients, including 16 meningioma, 6 trigeminal neurinoma, 2 pituitary adenoma, 2 craniopharyngioma, 1 facial neurinoma, and 1 metastatic tumor, underwent surgical treatment using skull base technique. Of theses, 16 tumors were mainly confined to middle cranial fossae, 5 tumors with extension into both anterior and middle fossa, and 7 tumors with extension into both middle and posterior fossa. Tumors that confined to the middle cranial fossa or extended into the anterior cranial fossa were operated with modified pterional, orbitozygomatic or Dolen'c approach, and tumors that extended into the posterior cranial fossa were operated with anterior, posterior or combined transpetrosal approach. Completeness of tumor resection, surgical outcome, postoperative complication, and follow up result were studied. Results : Total tumor removal was achieved in 9 tumors of 10 tumors that did not extended to the cavernous sinus, and was achieved in 7 tumors of 8 tumors that extended to the lateral wall of the cavernous sinus. Of 10 tumors that extended to the venous channel of the cavernous sinus, only 2 were removed totally. Surgical outcome was excellent in 14 patients, good in 10, fair in 2 and poor in 2. There were no death in this series. Dumbell type tumor which extended into both middle and posterior fossae showed tendency of poor prognosis as compared with tumors that confined middle cranial fossa and extended into both anterior and middle cranial fossa. Postoperative dysfunctions were trieminal hypesthesia in 3, oculomotor nerve palsy in 2, abducens nerve palsy in 2, hemiparesis in 2, cerebellar sign in 1, facial palsy in 1 and hearing impairment in 1. Conclusion : Based on our findings and a review of the literature, we conclude that, when selecting the surgical approach to the middle cranial fossa tumors, the most important factors to be considered were exact location of the tumor mass and existence of the cavernous sinus invasion by tumor mass. We recommend modified pterional or orbitozygomatic approach in cases with tumors located anterior and middle cranial base, without cavernous sinus invasion. In cases with tumors invading into cavernous sinus, we recommend Dolen'c or orbitozygomatic approach. And in lateral wall mass and the cavernous sinus, it is preferred to approach the tumor extradurally. For the tumor involing with middle fossa and posterior fossa(dumbell type) a combined petrosal approach is necessary. In cases with cavernous sinus invasion and internal carotid artery encasement, we recommend subtotal resection of the tumor and radiation therapy to prevent permanent postoperative sequele.

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An Application of Satellite Image Analysis to Visualize the Effects of Urban Green Areas on Temperature (위성영상을 이용한 도시녹지의 기온저감 효과 분석)

  • Yoon, Min-Ho;Ahn, Tong-Mahn
    • Journal of the Korean Institute of Landscape Architecture
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    • v.37 no.3
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    • pp.46-53
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    • 2009
  • Urbanization brings several changes to the natural environment. Its consequences can have a direct effect on climatic features, as in the Urban Heat Island Effect. One factor that directly affects the urban climate is the green area. In urban areas, vegetation is suppressed in order to accommodate manmade buildings and streets. In this paper we analyze the effect of green areas on the urban temperature in Seoul. The period selected for analysis was July 30th, 2007. The ground temperature was measured using Landsat TM satellite imagery. Land cover was calculated in terms of city area, water, bare soil, wet lands, grass lands, forest, and farmland. We extracted the surface temperature using the Linear Regression Model. Then, we did a regression analysis between air temperature at the Automatic Weather Station and surface temperature. Finally, we calculated the temperature decrease area and the population benefits from the green areas. Consequently, we determined that a green area with a radius of 500m will have a temperature reduction area of $67.33km^2$, in terms of urban area. This is 11.12% of Seoul's metropolitan area and 18.09% of the Seoul urban area. We can assume that about 1,892,000 people would be affected by this green area's temperature reduction. Also, we randomly chose 50 places to analysis a cross section of temperature reduction area. Temperature differences between the boundaries of green and urban areas are an average of $0.78^{\circ}C$. The highest temperature difference is $1.7^{\circ}C$, and the lowest temperature difference is $0.3^{\circ}C$. This study has demonstrated that we can understand how green areas truly affect air temperature.

A Study on the Characteristics of Each Section Based on Visitor's Satisfactions of the Dulegil in Bukhansan National Park (북한산국립공원 둘레길 탐방객 만족도에 따른 구간별 특성화 연구)

  • Han, Bong-Ho;Choi, Jin-Woo;Hur, Ji-Yeon;Kim, Sun-Hee;An, Kyung-Jin
    • Journal of the Korean Institute of Landscape Architecture
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    • v.41 no.2
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    • pp.69-82
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    • 2013
  • The purpose of this study is to evaluate the effect of Dulegil in Bukhansan National Park in dispersing peak climbing hikers, characterize each section of Dulegil and suggest ideas of improvement. This study was conducted based on the survey completed by visitors in all 21 sections of Dulegil. After the construction of Dulegil, the number of visit to Dulegil grew and it was analyzed that Dulegil attracted new visitors given that the rate of young people(aged 19~30) who visited for the first time was quite high. Regarding the frequency of peak climbing, 7.6% of the respondents said "decreased" and 46.2% said "increased", showing that Dulegil's effect to disperse peak climbing hikers is nominal. Seven qualities were evaluated regarding Dulegil's level of satisfaction. Out of those seven, the quality of recreational place and taking a walk achieved high scores of 3.74 and 3.61 respectively. The quality of culture and history scored the lowest with 3.09. The analysis on the characteristic of each section of Dulegil, reason of visit, and the visitors' level of satisfaction showed that Dulegil is now regarded as a place where they can improve their health through light exercise and walking. In addition, a positive effect can be expected for a long time since there are different ways of utilizing the resources of the National Park, such as getting in touch with nature, preserving ecology, learning history and enjoying beautiful landscapes. If infrastructure and programs specific to each section of Dulegil were improved in a long-term perspective, it would be effective to encourage peak climbers and enjoy the lower parts of the mountain.