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The Physicochemical Properties of $\alpha$-Amylase Inhibitors from Black Bean and Naked Barey in Korea (한국산 검정콩 및 쌀보리 $\alpha$-Amylase 저해물질의 이화학적 특성)

  • 심기환;문주석;배영일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.3
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    • pp.367-375
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    • 1998
  • The physicochemical properties of the $\alpha$-amylase inhibitors from black bean and naked barley is Korea were investigated. Preincubation time for maximum inhibition was 30min and no activity change was seen after that time. Optimum pH of the $\alpha$-amylase inhibitors from the black bean and naked barley was pH 7.0 and the inhibitory activities were stable in the range of pH 6.0~8.0 in both phosphate and Tris-HCI buffer solutions. Both inhibitors maintained more than 50% of activity after incubation for 17 min at 7$0^{\circ}C$. The inhibitors from the black bean and naked barley maintained more than 50% of activities after treatment for 40 min and 30 min with pepsin, and 30 min and 50 min with trypsin, respectively. Both inhibitors functioned via a noncompetitive mechanism and were active against porcine pancreatic and human salivary $\alpha$-amylases. The activities of both inhibitors were linear for the ionic stength ranging from 0 to 0.9. The addition of 70 mM maltose to the reaction mixture caused a maximum increase in the relative activities of both inhibitors, but it did not affect the dissociation of the EI complex. The activities of both inhibitors were significantly enhanced by adding 1mM of K+ or Mg2+.

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A Study on the Short Break Time on VDT Work using EMG (근전도를 이용한 VDT 작업시 짧은 휴식시간에 관한 연구)

  • Kim, Yu-Chang;Lee, Jun-Pal
    • Journal of the Ergonomics Society of Korea
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    • v.26 no.4
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    • pp.41-47
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    • 2007
  • This paper suggested the best work conditions including short break time and the number of mouse clicks on the computer work for the prevention of MSDs on VDT work. Fatigue measures included EMG based parameters. The short break time conditions are grouped into 7, 15, and 30 seconds after every work for 10 min and the number of mouse clicks are divided into 10, 20, and 30 clicks/min. The result of the ANOVA of the shift value of %MVC(Maximum Voluntary Contraction) showed the following: 1) There was a considerable difference as regards to the break time except the number of mouse clicks on the upper trapezius muscle(p$<$0.05). The best conditions were shown in 15 sec after every 10 min and 30 clicks/min. 2) There were considerable differences as regards to the number of mouse clicks except the break time on the extensor digitorum muscle and extensor carpi ulnaris muscle(p$<$0.05). The best conditions were shown in 7 sec after every 10min and 10 clicks/min.

A Study on the Life Prediction Method using Artificial Neural Network under Creep-Fatigue Interaction (인공 신경망을 이용한 크리프-피로 상호작용시 수명예측기법에 관한 연구)

  • 권영일;김범준;임병수
    • Transactions of the Korean Society of Automotive Engineers
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    • v.9 no.6
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    • pp.135-142
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    • 2001
  • The effect of tensile hold time on the creep-fatigue interaction in AISI 316 stainless steel was investigated. To study the fatigue characteristics of the material, strain controlled low cycle fatigue(LCF) tests were carried out under the continuous triangular waveshape with three different total strain ranges of 1.0%, 1.5% and 2.0%. To study the creep-fatigue interaction, 5min., 10min., and 30min. of tensile hold times were applied to the continuous triangular waveshape with the same three total strain ranges. The creep-fatigue life was found to be the longest when the 5min. tensile hold time was applied and was the shortest when the 30min. tensile hold time was applied. The cause fur the shortest creep-fatigue life under the 30min. tensile hold time is believed to be the effect of the increased creep damage per cycle as the hold time increases. The creep-fatigue life prediction using artificial neural network(ANN) showed closer prediction values to the experimental values than by the modified Coffin-Manson method.

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Synthesis of Ni-33.3at%Si Powders by MA and Their Sintering Characteristics (기계적 합금화에 의한 Ni-33.3at%Si 분말의 합성 및 소결 특성)

  • Park, Sang-Bo;Byeon, Chang-Seop;Kim, Dong-Gwan;Lee, Won-Hui
    • Korean Journal of Materials Research
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    • v.11 no.9
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    • pp.745-750
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    • 2001
  • Ni-33.3at%Si elemental powder mixtures were mechanically alloyed by a high-energy ball mill, followed by CIP (cold isostatic pressing) and HIP (hot isostatic pressing) for different processing conditions. Only elemental phases (Ni and Si) were observed for the 15 min mechanically alloyed (MA 15 min) powder. but $Ni_2$Si and elemental phases were observed to coexist for the 30 min mechanically alloyed (MA 30 min) powder. Elemental Ni and $Ni_2$Si phases were observed for the HIPed compact of MA 15 min powder at 100 and 150 MPa for 2 hr at $800^{\circ}C$. Only the $Ni_2$Si phase was, however, observed for the HIPed compacts of MA 30 min powder. For the HIPed compacts, the highest sintered density was obtained to be 99.5% of theoretical density by a HIP step at $1100^{\circ}C$ at 150MPa for 2hr. The hardness values of the HIPed $Ni_2$Si compacts at $1100^{\circ}C$ at 100/150 MPa for 2 hr were higher than HRC 66. The densification and mechanical property of HIPed $Ni_2$Si compacts were found to depend on more HIP temperature than HIP pressure.

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The Synthesis of Ti-37.5at%Si Powders by MA and Their Sintering Characteristics (기계적 합금화에 의한 Ti-37.5at%Si 분말의 합성 및 소결 특성)

  • 이상호;변창섭;김동관
    • Journal of Powder Materials
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    • v.8 no.4
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    • pp.223-230
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    • 2001
  • Ti-37.5at%Si elemental powder mixtures were mechanically alloyed by a high-energy ball mill, followed by CIP (cold isostatic pressing) and HIP (hot isostatic pressing) for different processing conditions. Only elemental phases (Ti and Si) were observed for the 5 min mechanically alloyed (MA 5 min) powder, but only $Ti_5Si_3$phase was observed for the 30 min mechanically alloyed (MA 30 min) powder. $Ti_5Si_3$phase was observed for the HIPed compact of MA 5 min and 30 min powders at 150 and 190 MPa for 3 hr at $1000^{\circ}C$. For the HIPed compacts, the highest sintered density was obtained to be 99.5% of theoretical density by a HIP step at $1350^{\circ}C$ at 190MPa for 3hr. The hardness values of the HIPed $Ti_5Si_3$compacts at $1350^{\circ}C$ at 150/190 MPa for 3hr were higher than HRC 76. The densification and mechanical property of HIPed $Ti_5Si_3$compacts was found to depend on more HIP temperature than HIP pressure.

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Fabrication of PbZrO$_3$ thin films crystal by sol-gel processing (Sol-Gel법에 의한 PbZrO$_3$박막 결정의 제작)

  • 전기범;김원보;배세환
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.10 no.3
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    • pp.211-218
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    • 2000
  • $PbZrO_3$precursor was prepared for the spin coating on the Pt/Ti/$SiO_2$/Si substrate. Two different heat treatment methods were used and the differencies were studied. One of the method is that the films were inserted into the furnace for 30 minutes and the other is that the films were annealed by rapid thermal annealing (RTA) for 1 minute at the same temperatures. We also examined the tendency of crystallization by annealing at the fixed temperature, $700^{\circ}C$ as a function of time, namely during 1, 10, 20, and 30 minitues, respectively. The optimum conditions for the crystallization of these films were at $550^{\circ}C$ during 30 min. and at $700^{\circ}C$ during 10 min. in muffle furnace and at $650^{\circ}C$ during 1 min in RTA furnace. The best condition for making good quality grains needs 30 min. at $700^{\circ}C$.

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Effect of Pre-cooking Conditions on the Quality Characteristics of Ready-To-Eat Samgyetang

  • Triyannanto, Endy;Lee, Keun Taik
    • Food Science of Animal Resources
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    • v.35 no.4
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    • pp.494-501
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    • 2015
  • The aim of this study was to examine the effectiveness of pre-cooking conditions on the quality characteristics of ready-to-eat (RTE) Samgyetang. Raw chickens were steamed under the different conditions of 50℃/30 min (T1), 65℃/30 min (T2), 85℃/30 min (T3), and 90℃/10 min (T4) prior to retorting at 120℃ for 65 min. The results showed that pre-cooking conditions in all treated samples could reduce fat contents in breast and leg meats by 8.5-11.7% and 10.0-11.0% compared to the control, even though there were no significant differences among treatments (p>0.05). The L* and b* values of breast and leg meats treated with the higher temperature and longer time conditions were significantly higher than the control (p<0.05), while a* values tended to decrease despite of not to a significant extent (p>0.05). Moreover, apparent viscosity and water soluble protein showed insignificant differences (p>0.05) among the samples as a result of the retorting process, which might have more negative influences on the quality. T2 samples obtained significantly the highest average Quantitative Descriptive Analysis (QDA) score and transmittance value, representing the most clear broth among the samples, compared to the control. On the other hand, T3 showed the highest cooking loss among the treatments and the lowest QDA scores among the samples. In conclusion, pre-cooking treatment prior to retorting in manufacturing Samgyetang is a plausible way to reduce its fat content. A pre-cooking condition at either 65℃ for 30 min, or 90℃ for 10 min are recommended for producing Samgyetang with optimum quality.

Effect of Ambient Temperature on the AC Electrical Treeing Phenomena in an Epoxy/Layered Silicate Nanocomposite

  • Park, Jae-Jun
    • Transactions on Electrical and Electronic Materials
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    • v.14 no.4
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    • pp.221-224
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    • 2013
  • Effects of ambient temperature on the ac electrical treeing and breakdown behaviors in an epoxy/layered silicate (1 wt%) were carried out in needle-plate electrode geometry. A layered silicate was exfoliated in an epoxy base resin,, using our ac electric field apparatus. To measure the treeing initiation and propagation, and the breakdown rate, constant alternating current (ac) of 10 kV (60 Hz) was applied to the specimen in a needle-plate electrode arrangement, at $30^{\circ}C$, $90^{\circ}C$ or $130^{\circ}C$ of insulating oil bath. At $30^{\circ}C$, the treeing initiation time and the breakdown time in the epoxy/layered silicate (1 wt%) system were 1.4 times higher than those of the neat epoxy resin. At $90^{\circ}C$ (lower than Tg), electrical treeing was initiated in 55 min, and propagated until 1,390 min at the speed of $0.35{\times}10^{-3}mm/min$, which was 4.4 times higher than that at $30^{\circ}C$; however, there was almost no further treeing propagation after 1,390 min. At $130^{\circ}C$ (higher than Tg), electrical treeing was initiated in 44 min, and propagated until 2,000 min at the speed of $0.96{\times}10^{-3}mm/min$. Typical branch type electrical treeing was obtained from the neat epoxy and epoxy/layered silicate at $30^{\circ}C$, while bush type treeing was observed out from the needle tip at $90^{\circ}C$ and $130^{\circ}C$.

A study on the titanium welding process according to the temperature characteristics of shielding methods (티타늄 용접공정에서 온도특성에 따른 실딩방법에 관한 연구)

  • Chung, Han-Shik;Jeong, Hyo-Min;Lee, Dae-Chul;Lee, Byeong-Yong
    • Journal of Advanced Marine Engineering and Technology
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    • v.37 no.1
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    • pp.29-34
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    • 2013
  • The welds tent to be weakened if it is exposed to the air during the welding process. In titanium welding with Gas Tungsten Arc Welding, inappropriate colors of the welds, such as purple/red, blue, yellow, gray, white and pink represents that it is contaminated by oxygen and nitrogen in the air. Shielding gases can be used to protect welds from the contamination. In addition, Weld metal and heat affected zone (HAZ) are also shielded from the air when it is cooled down to the room temperature. In this paper, appropriate shielding gases for the shape and form of Trailing Shielding Jig and torch shielding ($13{\sim}20{\ell}/min$), after shielding ($22{\sim}30{\ell}/min$), and back shielding ($25{\sim}30{\ell}/min$) are studied.

Separation Characteristics of Whey Protein by High Performance Membrane Chromatography (고성능 막 크로마토그래피에 의한 유청 단백질의 분리특성)

  • 홍승범;노경호
    • KSBB Journal
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    • v.16 no.6
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    • pp.533-537
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    • 2001
  • ${\alpha}$-lactalbumin and ${\beta}$-lactoglobulin in whey proteins were separated by high performance membrane chromatography (HPMC). The separation mechanism involved anion-exchange, and the stationary phase was anion CIM (Convective Interaction Media) DEAE, QA disk and cation exchanger SO$_3$(16${\times}$3 mm). Two types of mobile phase were used, buffer A (20 mM Tris-HCI, pH 7.3) and buffer B(buffer A + 1 M NaCl), As the amount of NaCl dissolved in buffer linearly increased, which enabled a gradient elution mode. The optimum mobile phase and operating condition (Buffer A/Buffer B = 100/0 - 30/70 vol%, gradient time 1 min, 30/70 - 10/90 vol.%, gradient time 2 min) were experimentally determined. In this experimental condition, ${\alpha}$-lacta1bumin, ${\beta}$-lactoglobulin were separated within 5 min at a mobile phase flow rate of 4 mL/min.

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