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The Effects of Electrical Stimulation on the Change of Oxytocin Immunoreactive cells (침전극 저주파자극이 옥시토신 면역반응세포 변화에 미치는 영향)

  • Kim, Soo-Han;Kim, Soon-Hee
    • Journal of Korean Physical Therapy Science
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    • v.9 no.4
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    • pp.53-61
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    • 2002
  • This study was carried out to investigate the effects of electrical stimulation on the change oxytocin(OT) immunoreactive cells in the hypothalamus of the male SD rats. Fifteen healthy and normal male rats were divided into three groups, 5 SD rat in each group. The one group has been stimulated by electro-acupuncture(EA, 2Hz) for 30 min and the other group by EA for 1hr 30 min and control group has not been stimulated. The results were summarized as follows. OT immunoreactive cells were found in the Paraventricular nucleus (PVN), Supraoptic nucleus(SON) and Lateral Magnocellular Area(LMA). The number of OT immunoreactive cells of PVN were significantly increased after 30 min as compared with control group (p<0.05) and were also significantly increased after 1hr 30min than 30 min(p<0.05). The number of OT immunoreactive cells of SON were significantly increased after 30 min and 1hr 30 min(p<0.05) as compared with control group, but decreased after 1hr 30 min than 30 min. The number of immunoreactive hells of LMA significantly increased after 30 min(p<0.05) as compared with control group, but significantly decreased after 1hr 30 min than 30 min(p<0.05). These results show that OT is released into the hypothalamus in response to electrical stimulation.

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Effects of Invasive Low Level Laser Acupuncture Therapy(LLLAT) depending on capacity at LU7+LR5 on the Hyperlipemia Rats Induced by High Fat Diet (열결(列缺).려구에 대한 용량별 He-Ne 레이저 침 조사(照射)가 고지방(高脂肪) 식이(食餌)로 유발된 고지혈증(高脂血症) 백서(白鼠)에 미치는 영향(影響))

  • Bang, Sang-Hee;Youn, Dae-Hwan;Cho, Myung-Re;Yim, Hyun-Jin;Na, Chang-Su
    • Korean Journal of Acupuncture
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    • v.23 no.1
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    • pp.1-14
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    • 2006
  • Objectives : This research was performed to investigate the effect of invasive low level laser acupuncture therapy(LLLAT) at Yolgyol+yogu(LU7+LR5) on weight gain, food intake, food efficiency, lipid metabolism and liver function in hyperlipemia rats. Methods : Experimental groups were divided into high fat diet cup(Control group), high fat diet and 15 mW/5 min LLLAT at LU7+LR5(15 mW/5 min group), 15 mW/10 min LLLAT at LU7+LR5(15 mW/10 min group), 30 mW/5 min LLLAT at LU7+LRS(30 mW/5 min group), 30 mW/10 min LLLAT at LU7+LR5(30 mW/10 min group). LLLAT was treated at the 15,30mW-5,10min once a 2day during 5 weeks. Results : Body weight was decreased significantly in 30 mW/s min group and 30 mW/10 min group compared with control group. Food intake was decreased significantly in 15 mW/5 min group and 15 mW/10 min group compared with control group. Food efficiency was decreased significantly in 30 mW/10 min group compared with control group. In the lipid metabolism, total cholesterol, HDL-cholesterol and free fatty acid were increased significantly in 15 mW/5 min group and 30 mW/10 min group compared with control group. LDL-cholesterol was increased in 30 mW/10 min group and phospholipids was decreased significantly in all experimental group compared with control group. triglyceride was not showed significant result in all experimental group. In the liver function, AST was not showed significant result in all experimental group, ALT and ALP were increased significantly in 30 mW/10 min group, ALP was decreased in 30 mW/5 min group compared with control group. Conclusions : LLLAT at LU7 and LR5 can manage hyperlipemia by controlling body weight, food intake, food efficiency ratio and lipid metabolism.

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Response of Electrocardiogram of Nile tilapia, Oreochromis niloticus to Electric Stimulus (전기자재에 대한 역돔의 심전도)

  • 한규환;양용림
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.38 no.4
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    • pp.278-283
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    • 2002
  • The response of electrocardiogram(ECG) of Nile tilapia, Oreochromis niloticus [Linnaeus] was studied to the electric stimulus which was given to a certain part of body The experiments were performed in such a way that three levels of electric stimulus (20, 30, 40 Vp ; 10 msec) were given to fishes with electrode inserted into their bodies and then their ECGs were recorded continuously for 60 minutes in the water temperature of 16~18$^{\circ}C$ The results of the experiments were divided by day and night, and then were analyzed by experimental conditions as follows; 1. Nile tilapia reached a stable condition within 3 minutes after the electrode inserted into their bodies during anesthesia. In stable condition, the heart rates average was 45.8 beat/min during daytime and 45.0 beat/min at night. The action potentials average was 1.76 $mutextrm{V}$during daytime and 1.75 $mutextrm{V}$ at night. 2. The heart rates average by three levels of electric stimulus were \circled1 In the stimulus condition, the heart rates were 34.9 beat/min during daytime and 33.4 beat/min at night for the 20 Vp level, 36.8 bea/min during daytime and 36.0 beat/min at night for the 30 Vp level, and 38.0 beat/min during daytime and 36.4 beat/min at night for the 40Vp level. \circled2 In the recovery condition, the action potentials were 45.5 beat/min during daytime an 45.1 beat/min at night for the 20Vp level, 47.9 beat/min during daytime and 49.0 beat/min at night for the 30Vp level, and 51.4 beat/min during daytime and 50.7 beat/min at night for the 40Vp level 3. The action potentials average by three levels of electric stimulus were, \circled1 In the stimulus condition, action potentials were 2.54 $mutextrm{V}$ during daytime and 2.39 $mutextrm{V}$ at night for the 20 Vp level, 3.30 $mutextrm{V}$ during daytime and 2.30 $mutextrm{V}$ at night for the 30 Vp level and 6.05 $mutextrm{V}$ during daytime and 3.23 $mutextrm{V}$ at night for the 40 Vp level. \circled2 In the recovery condition, action potentials were 1.92 $mutextrm{V}$ during daytime and 1.95 $mutextrm{V}$ at night for the 20 Vp level and 2.78 $mutextrm{V}$ during daytime and 2.21 $mutextrm{V}$ at night for the 30Vp level and 3.6 0 $mutextrm{V}$ during daytime and 2.98 $mutextrm{V}$ at night for the 40 Vp level.

Fuel Properities of Spent Coffee Bean by Torrefaction (반탄화에 의한 커피박 연료특성)

  • Oh, Dohgun;Kim, Yonghyun;Son, Hong-Seok
    • New & Renewable Energy
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    • v.9 no.3
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    • pp.29-35
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    • 2013
  • This research analyzed the fuel characteristic change of spent coffee bean by torrefaction. The calorific value was increased from 4,974 kcal/kg to 6,075 kcal/kg ($260^{\circ}C$, 30min), 6,452 kcal/kg ($270^{\circ}C$, 30min), 6,823 kcal/kg ($280^{\circ}C$, 30min), 6,970 kcal/kg ($260^{\circ}C$, 30min). The highest energy yield was obtained when the spent coffee bean were torrefied on the condition of $280^{\circ}C$, 30min. The moisture absorption rate was decreased from 5.12% to 2.76% when the spent coffee bean were torrefied on the condition of $290^{\circ}C$, 30min. Lignin was increased from 11.33% to 14.39% on the condition of $260^{\circ}C$ 30min. But it did not preferability to torrefy spent coffee bean at temperature of more than $270^{\circ}C$ because lignin decreases to the level that is hard to make pellet.

Studies on the Changes in Chemical Composition and Microbiological Aspects of Raw Milk by Microwave Heating (Microwave 열처리에 의한 원유의 화학적 및 미생물학적 성상의 변화에 관한 연구)

  • Shin, Byeong Hong;Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.25 no.2
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    • pp.181-198
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    • 1998
  • In order to determine the optimum pasteurization conditions by microwave heating(MWH) at $50^{\circ}C{\sim}70^{\circ}C$ for 30 minute compared with water bath heating(WBH) at $65^{\circ}C$ for 30minute during storage at $5^{\circ}C$, the chemical composition, microbiological changes and keeping quality were examined and the results were as follows: 1. The fat protein lactose, total solid contents of raw milk, at $50{\sim}70^{\circ}C$ for 30 min. in MWH and at 65 for $30^{\circ}C$ min. in WBH did not changed significantly during the storage at $5^{\circ}C$. 2. The pH and acidity for the raw milk untreated were 6.75 and 0.16%, and those of MWH heated and WBH milk wee 6.75~6.50 and 0.16%~0.19%, phosphatase test were negative at $61^{\circ}C$ for 20 min. at $62^{\circ}C$ for 15 min. at $63^{\circ}C$ for 10 min. at $64^{\circ}C$ for 5 min. at $65^{\circ}C$ for 5 min. in MWH and at $65^{\circ}C$ for 30 min. in WBH. 3. Whey protein content was $18.53mg/m{\ell}$ in raw milk untreated, however, those were decreased as the heating temperature increased. The proteolytic activity of treated milk by WBH(44%) was lower than that by MWH(94%). 4. Total bacteria counts were $2.8{\times}10^5CFU/m{\ell}$ in raw milk untreated, $2.8{\times}10^3CFU/m{\ell}$ at $65^{\circ}C$ for 30 min. $2.4{\times}10^3CFU/m{\ell}$ at $70^{\circ}C$ for 30 min. in MWH and $3.0{\times}10^3CFU/m{\ell}$ at $65^{\circ}C$ for 30 min. in WBH. Because total bacteria count did not increased in MWH at $65^{\circ}C$, $70^{\circ}C$ for 30 min. and $65^{\circ}C$ for 30 min. in WBH during the 10 days storaging, Also, total bacteria counts for treated milk were a most drastic decrease after $61^{\circ}C$, $62^{\circ}C$, $63^{\circ}C$, $64^{\circ}C$, $65^{\circ}C$ for 5 min. in MWH. 5. Coliform bacteria counts were $2.6{\times}10^3CFU/m{\ell}$ in raw milk untreated. There were not detected at $55^{\circ}C{\sim}70^{\circ}C$ for 30 min. in MWH and at $65^{\circ}C$ for 30 min. in WBH. Coliform bacteria counts were not detected after $61^{\circ}C$, $62^{\circ}C$, $63^{\circ}C$, $64^{\circ}C$, $65^{\circ}C$ for 5 min. in MWH. 6. Thermoduric bacteria counts were $5.2{\times}10^4CFU/m{\ell}$ in raw milk untreated, $2.0{\times}10^3CFU/m{\ell}$ at $65^{\circ}C$ for 30 min. $1.9{\times}10^3CFU/m{\ell}$ at $70^{\circ}C$ for 30min. in MWH and $2.2{\times}10^3CFU/m{\ell}$ at $65^{\circ}C$ for 30 min. in WBH. Because thermoduric bacteria counts did not increased in MWH at $65^{\circ}C$, $70^{\circ}C$ for 30 min. and $65^{\circ}C$ for 30 min. in WBH during the 10days storaging. Also, thermoduric bacteria counts were a most drastic decrease after $61^{\circ}C$, $62^{\circ}C$, $63^{\circ}C$, $64^{\circ}C$, $65^{\circ}C$ for 5 min. in MWH. 7. Psychrotrophic bacteria counts were $2.8{\times}10^5CFU/m{\ell}$ in raw milk untreated, $2.0{\times}10^1CFU/m{\ell}$ at $65^{\circ}C$ for 30 min. $2.0{\times}10^1CFU/m{\ell}$ at $70^{\circ}C$ for 30 min. in MWH and $3.0{\times}10^1CFU/m{\ell}$ at $65^{\circ}C$for 30 min. in WBH. Because psychrotrophic bacteria counts did not increased in MWH at $65^{\circ}C$, $70^{\circ}C$ for 30min. and $65^{\circ}C$ for 30 min. in WBH during the 10 days storaging. Also, psychrotrophic bacteria counts were a most drastic decrease after $61^{\circ}C$, $62^{\circ}C$, $63^{\circ}C$, $64^{\circ}C$, $65^{\circ}C$ for 5 min. in MWH.

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Heavy Metals Extraction from Contaminated Soils using Aqua Regia Extraction (왕수를 이용한 중금속 오염토양에서의 추출방안 연구)

  • Lee, Dukyoung;Jung, Sunkook;Son, Younggyu
    • Journal of Soil and Groundwater Environment
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    • v.23 no.2
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    • pp.15-22
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    • 2018
  • Aqua regia extraction for the quantification of heavy metals and As in contaminated soils was investigated as one of preliminary steps for on-site monitoring using sensor technology. Soil samples were taken from closed railway depot, closed mines, and closed refinery and various extraction conditions including $30^{\circ}C/15min$, $30^{\circ}C/30min$, $30^{\circ}C/60min$, $30^{\circ}C/120min$, $80^{\circ}C/15min$, $80^{\circ}C/30min$, $80^{\circ}C/60min$, $80^{\circ}C/120min$ were tested. The optimal extraction condition was determined as $80^{\circ}C/60min$ because the extraction efficiencies of Zn were relatively low and did not reach the targeted level (80-100% of original concentrations) for $30^{\circ}C$ conditions. It was found that the fractionation of heavy metals and As using the sequential extraction method was useful to understand the degree of metal extraction. In order to enhance the extraction efficiency within short extraction time, ultrasound technology using a 20 kHz horn-type sonicator was additionally used for $30^{\circ}C/15min$. It was revealed that ultrasound could significantly enhance the extraction efficiency and pulsed irradiation showed higher efficiency than continuous irradiation due to the less formation of bubble clouds. However high temperature condition ($80^{\circ}C$) was required to achieve high extraction efficiency for Zn in spite of the use of ultrasound.

The Effect of 30% Oxygen on the Memory Performance, Hyperoxia and Heart Rate (30% 농도의 산소 공급이 기억력, 혈중 산소 농도, 심박률에 미치는 영향)

  • Chung, Soon-Cheol;Tack, Gye-Rae;Lee, Bong-Soo
    • IE interfaces
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    • v.18 no.2
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    • pp.178-183
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    • 2005
  • In this study, changes in memory performance, blood oxygen saturation and heart rate according to 30% concentration oxygen supply were observed. Ten healthy male and female college students (male:$25.8{\pm}0.8$ years, female: $24.2{\pm}1.9$ years) participated in the study. The experiment was performed as Rest (1min.), Control task (1min.), Word presentation (1min.), Reaction time task (1min.), Distractor (2min.), and Word recall (1min.) and the physiological signals such as blood oxygen saturation and heart rate were measured throughout the stages. Subjects who received 30% oxygen recalled more words than those who received 21% oxygen, which shows 30% oxygen supply has influenced positively on memory cognitive performance. When 30% concentration oxygen is supplied, the blood oxygen saturation in the task phases was increased and the heart rate decreased when comparing to 21%. It means that 30% oxygen can stimulate brain activation by increasing actual blood oxygen concentration in the process of cognitive performance, and the heart rate decreases because enough oxygen is supplied to process the cognitive performance.

Reverse effects of electroacupuncture on xylazine analgesia in goats (염소의 xylazine 진정(鎭靜)에 대한 전침자극(電針刺戟)의 길항효과(拮抗效果))

  • Seo, Kang-moon;Yoon, Jung-hee;Cheong, Jong-tae;Chang, Kwang-ho;Nam, Tchi-chou
    • Korean Journal of Veterinary Research
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    • v.32 no.4
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    • pp.683-688
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    • 1992
  • This study was performed to investigate the reverse effects of Jen Chung electroacupuncture at 10 and 30 min after xylazine administration(0.1mg/kg) in goats. In the group of electroacupuncture at 30 min after xylazine administration, MAT and MWT were markedly shorter than in control and in 10 min after xylazine administration. The RR intervals were continuously increased in control and in the group of electroacupuncture at 10 min after xylazine administration, but in the group of electroacapuncture at 30 min after xylazine administration significantly reduced from about 45 min. The frequency(cps) of EEG was recovered to normal pattern after 90 min in the group of electroacupuncture at 30 min after xylazine administration, but considerably delayed in control and in the group of electroacupuncture at 10 min. Respiratory rates tended to be somewhat decreased and then gradually increased in all groups. In control, there were shown cycloapneustic respirations but no in the electroacupuncture groups. It was turned out to be that the electroacupuncture stimulation of Jen Chung at 30 min after xylazine administration was effective for the reverse of the xylazine analgesia.

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Effect of Renal Ischemia in Tetraethylammonium Transport in Rabbit Renal Coritcal Slices

  • Joo, Woo-Sik;Nam, Yun-Jeong;Jung, Jin-Sup;Kim, Yong-Keun
    • The Korean Journal of Physiology
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    • v.25 no.2
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    • pp.171-177
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    • 1991
  • This study was carried out to determine effect of acute renal ischemia on transport function of organic cation, tetraethylammonium (TEA), in rabbit kidney proximal tubule. Clamping of the renal artery for 30 and 60 min produced a polyuria which was accompanied by an increase in $Na^+$ excretion. The capacity of kidney cortical slices to accumulate TEA was increased after 30 and 60 min of ischemia. When blood flow was restored for 30 min after 30 and 60 min of ischemia, the augmented TEA uptake was recovered to the control values. Oxygen consumption of cortical slices was stimulated after 30 min of ischemia, whereas it was not altered by 60 min of ischemia. A 90-min ischemia produced a significant inhibition of TEA uptake and tissue oxygen consumption. These results suggest that the basolateral transport system for organic cation persists after ischemic periods of 60 min despite evidence that tubular reabsorptive mechanism of $Na^+$ and water is markedly impaired. This may indicate that the active secretory systems of proximal tubule are more resistant to ischemic injury than the reabsorptive systems.

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Determination of Proper Loading Speed for Deformation Strength Test of Asphalt Concretes (아스팔트 콘크리트 변형강도 시험에서의 적정 하중재하속도 선정 연구)

  • Cho, Byung-J.;Park, Tae-W.;Doh, Young-S.;Kim, Kwang-W.
    • International Journal of Highway Engineering
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    • v.10 no.4
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    • pp.225-234
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    • 2008
  • This study was carried out to select proper loading speed for deformation strength ($S_D$) of asphalt mixtures. Kim test using loading head of diameter(40mm) with radius(10mm) was conducted to measure $S_D$ in different loading speed (10mm/min, 30mm/min, 50mm/min, 70mm/min) and wheel tracking test was also conducted. The regression analyses between the So values and WT results were carried out by loading speeds. Higher $S_D$ was observed as increasing loading speed. This means that loading speed is a high influencing factor on $S_D$. The loading speed of 30mm/min was found as an optimum for better correlation with WT results than any other speeds from the regression analysis between $S_D$ and wheel tracking test results. $S_D$ value measured at other loading speed than 30mm/min has to apply the conversion coefficients.

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