Response of Electrocardiogram of Nile tilapia, Oreochromis niloticus to Electric Stimulus

The response of electrocardiogram(ECG) of Nile tilapia, Oreochromis niloticus [Linnaeus] was studied to the electric stimulus which was given to a certain part of body The experiments were performed in such a way that three levels of electric stimulus (20, 30, 40 Vp ； 10 msec) were given to fishes with electrode inserted into their bodies and then their ECGs were recorded continuously for 60 minutes in the water temperature of 16~18$^{\circ}C$ The results of the experiments were divided by day and night, and then were analyzed by experimental conditions as follows; 1. Nile tilapia reached a stable condition within 3 minutes after the electrode inserted into their bodies during anesthesia. In stable condition, the heart rates average was 45.8 beat/min during daytime and 45.0 beat/min at night. The action potentials average was 1.76 $mutextrm{V}$during daytime and 1.75 $mutextrm{V}$ at night. 2. The heart rates average by three levels of electric stimulus were \circled1 In the stimulus condition, the heart rates were 34.9 beat/min during daytime and 33.4 beat/min at night for the 20 Vp level, 36.8 bea/min during daytime and 36.0 beat/min at night for the 30 Vp level, and 38.0 beat/min during daytime and 36.4 beat/min at night for the 40Vp level. \circled2 In the recovery condition, the action potentials were 45.5 beat/min during daytime an 45.1 beat/min at night for the 20Vp level, 47.9 beat/min during daytime and 49.0 beat/min at night for the 30Vp level, and 51.4 beat/min during daytime and 50.7 beat/min at night for the 40Vp level 3. The action potentials average by three levels of electric stimulus were, \circled1 In the stimulus condition, action potentials were 2.54 $mutextrm{V}$ during daytime and 2.39 $mutextrm{V}$ at night for the 20 Vp level, 3.30 $mutextrm{V}$ during daytime and 2.30 $mutextrm{V}$ at night for the 30 Vp level and 6.05 $mutextrm{V}$ during daytime and 3.23 $mutextrm{V}$ at night for the 40 Vp level. \circled2 In the recovery condition, action potentials were 1.92 $mutextrm{V}$ during daytime and 1.95 $mutextrm{V}$ at night for the 20 Vp level and 2.78 $mutextrm{V}$ during daytime and 2.21 $mutextrm{V}$ at night for the 30Vp level and 3.6 0 $mutextrm{V}$ during daytime and 2.98 $mutextrm{V}$ at night for the 40 Vp level.

전기자재에 대한 역돔의 심전도

전기자극에 대한 역돔, Oreochromis niloticus［Linnaeus］의 심전도를 구명하기 위하여, 어체내에 전극을 삽입하여 3 가지 전기자극원(20, 30, 40 Vp ; 10 msec)으로 실험어에 자극을 가하였을 때의 심전도를 서간과 야간으로 구분하여 60분간 조사한 심박수와 생체전위를 자극 전(마취상태, 안정상태)과 자극 후(자극-회복상태, 회복상태)로 구분하여 비교 분석한 결과는 다음과 같다. 1. 역돔은 마취 후 3 분 뒤에 안정상태에 도달하였고, 안정상태에서의 평균상심박수는 서간에 45.8 beat/min, 야간에 45.0 beat/min였고, 평균생체전위는 서간에 1.76 $\mu\textrm{V}$, 야간에 1.75 $\mu\textrm{V}$였다. 2. 자극별 평균심박수는 \circled1 자극-회복상태에서 전기자극이 20 Vp인 경우, 서간에 34.9 beat/min, 야간에 33.4 beat/min였고, 30 Vp인 경우, 서간에 36.8 beat/min, 야간에 36.0 beat/min였으며, 40Vp인 경우 서간에 38.0 beat/min, 야간에 36.4 beat/min로 나타났다. \circled2 회복상태에서 전기자극이 20 Vp인 경우, 서간에 45.5 beat/min, 야간에 45.1 beat/min였고, 30 Vp인 경우, 서간에 47.9 beat/min, 야간에 49.0 beat/min 였으며, 40 Vp인 경우, 서간에 51.4 beat/min, 야간에 50.7 beat/min로 나타났다. 3. 자극별 평균생체전위는 \circled1 자극-회복상태에서 전기자극이 20Vp인 경우, 서간에 2.54$\mu\textrm{V}$ 야간에 2.39$\mu\textrm{V}$였고,30Vp인 경우, 서간에 3.30$\mu\textrm{V}$, 야간에 2.30 $\mu\textrm{V}$였으며, 40Vp 인 경우 서간에 6.05 $\mu\textrm{V}$, 야간에 3.23 $\mu\textrm{V}$로 나타났다. \circled2 회복상태에서 전기자극이 20 Vp인 경우, 서간에 1.92 $\mu\textrm{V}$, 야간에 1.95 $\mu\textrm{V}$ 였고, 30 Vp인 경우, 서간에 2.78 $\mu\textrm{V}$, 야간에 2.21 $\mu\textrm{V}$ 였으며, 40 Vp인 경우 서간에 3.60$\mu\textrm{V}$ 야간에 2.98$\mu\textrm{V}$로 나타났다.