• Molecules and Cells
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• v.43 no.1
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• pp.76-85
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• 2020

MARCH5 is a RING finger E3 ligase involved in mitochondrial integrity, cellular protein homeostasis, and the regulation of mitochondrial fusion and fission. To determine the function of MARCH5 during development, we assessed transcript expression in zebrafish embryos. We found that march5 transcripts were of maternal origin and evenly distributed at the 1-cell stage, except for the mid-blastula transition, with expression predominantly in the developing central nervous system at later stages of embryogenesis. Overexpression of march5 impaired convergent extension movement during gastrulation, resulting in reduced patterning along the dorsoventral axis and alterations in the ventral cell types. Overexpression and knockdown of march5 disrupted the organization of the developing telencephalon and diencephalon. Lastly, we found that the transcription of march5 was tightly regulated by the transcriptional regulators CHOP, C/EBPα, Staf, Znf143a, and Znf76. These results demonstrate the essential role of March5 in the development of zebrafish embryos.

• Journal of The Geomorphological Association of Korea
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• v.24 no.2
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• pp.15-25
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• 2017

Subi basin is located at the crestline of Taebaek mountains. This paper aims to elucidate the geomorphic development of Subi basin through the analysis of river terraces built in Wangpi-cheon and Banbyeon-cheon. Wangpi-cheon flows northeastward from Subi basin, and Banbyeon-cheon flows southward at the west of Subi basin. Absolute age of terrace is measured by means of OSL methodology, long profile of Wangpi-cheon is made up with 10m interval contour line, and the elevation above river bed of high terraces is measured at the end part of terrace. The results are as follow: Firstly, high river terraces of Subi basin, Wangpi-cheon and Banbyeon-cheon are formed about 40 kyr(MIS 3) being interstadial stage of last glacial period. Secondly, the elevation above river bed of high terraces of Wangpi-cheon and Banbyeon-cheon tends to increase toward upstream. It means that the uplift of Taebaek Mountains influences considerably the formation of their terraces. Thirdly, the elevation above river bed of high terraces at the reach from Seomchon to Suha-ri of Wangpi-cheon tends to decrease toward upstream. This section is captured from Banbyeon-cheon flowing in the opposite direction. River piracy has occurred from the time of formation of Suha-ri high terrace to the time of formation of Hantee wind gap. Finally, for fluvial system of Wangpi-cheon to establish dynamic equilibrium, topographic axis will move toward Banbyeon-cheon.

• Journal of the Earthquake Engineering Society of Korea
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• v.25 no.5
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• pp.191-199
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• 2021

In seismic design, hollow section concrete columns offer advantages by reducing the weight and seismic mass compared to concrete section RC bridge columns. However, the flexure-shear behavior and spirals strain of hollow section concrete columns are not well-understood. Octagonal RC bridge columns of a small-scale model were tested under cyclic lateral load with constant axial load. The volumetric ratio of the transverse spiral hoop of all specimens is 0.00206. The test results showed that the structural performance of the hollow specimen, such as the initial crack pattern, initial stiffness, and diagonal crack pattern, was comparable to that of the solid specimen. However, the lateral strength and ultimate displacement of the hollow specimen noticeably decreased after the drift ratio of 3%. The columns showed flexure-shear failure at the final stage. Analytical and experimental investigations are presented in this study to understand a correlation confinement steel ratio with neutral axis and a correlation between the strain of spirals and the shear resistance capacity of steel in hollow and solid section concrete columns. Furthermore, shear strength components (V_c, V_s·, V_p) and concrete stress were investigated.

• Molecules and Cells
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• v.41 no.9
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• pp.868-880
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• 2018

Pancreatic cancer (PC) is one of the most aggressive cancers presenting with high rates of invasion and metastasis, and unfavorable prognoses. The current study aims to investigate whether EZH2/miR-139-5p axis affects epithelial-mesenchymal transition (EMT) and lymph node metastasis (LNM) in PC, and the mechanism how EZH2 regulates miR-139-5p. Human PC and adjacent normal tissues were collected to determine expression of EZH2 and miR-139-5p, and their relationship with clinicopathological features of PC. Human PC cell line was selected, and treated with miR-139-5p mimics/inhibitors, EZH2 vector or shEZH2 in order to validate the regulation of EZH2-mediated miR-139-5p in PC cells. Dual-luciferase report gene assay and chromatin immunoprecipitation assay were employed to identify the relationship between miR-139-5p and EZH2. RT-qPCR and Western blot analysis were conducted to determine the expression of miR-139-5p, EZH2 and EMT-related markers and ZEB1/2. Tumor formation ability and in vitro cell activity were also analyzed. Highly-expressed EZH2 and poorly-expressed miR-139-5p were detected in PC tissues, and miR-139-5p and EZH2 expressions were associated with patients at Stage III/IV, with LNM and highly-differentiated tumors. EZH2 suppressed the expression of miR-139-5p through up-regulating Histone 3 Lysine 27 Trimethylation (H3K27me3). EMT, cell proliferation, migration and invasion were impeded, and tumor formation and LNM were reduced in PC cells transfected with miR-139-5p mimics and shEZH2. MiR-139-5p transcription is inhibited by EZH2 through up-regulating H3K27me3, thereby down-regulation of EZH2 and up-regulation of miR-139-5p impede EMT and LNM in PC. In addition, the EZH2/miR-139-5p axis presents as a promising therapeutic strategy for the treatment of PC.

• Journal of Korean Society of Forest Science
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• v.102 no.2
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• pp.305-308
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• 2013

This study was conducted to describe the external morphology of Platypus-koryoensis Murayama in immature stage, to determine each instar based on larvae head-capsule, and to measure individual and local variations in the number of mycangia of adult. Egg of P. koryoensis, had oval shape and lengths of the major and minor axis were $0.67{\pm}0.051$ mm and $0.41{\pm}0.053$ mm, respectively. Body colour of $5^{th}$ instar was gloss white with well-developed mandible. Larvae of P. koryoensis grew up to $5^{th}$ instar and each instar was clearly classified by head capsule width. Head capsule width for $1^{st}$, $2^{nd}$, $3^{rd}$, $4^{th}$, and $5^{th}$ instar larvae were $0.35{\pm}0.004$ mm, $0.45{\pm}0.010$ mm, $0.67{\pm}0.039$ mm, $0.94{\pm}0.069$ mm and, $1.12{\pm}0.007$ mm, respectively. The colour of pupa was pale yellow and its length was $4.64{\pm}0.044$ mm. The number of mycangia per female had individual variations from 5 to 12 and 83% of the adults had from 6 to 8 mycangia. There was no significant difference in number of mycangia collected from between Namyangju region and Honchen region.

• Korean Journal of Applied Biomechanics
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• v.16 no.4
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• pp.115-124
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• 2006

This study was conducted to investigate the running motion in the max-velocity phase(150-160m) and the fatigue phase(350-360m) during 400m sprint by performed elementary school athletes. Eighteen elementary school male athletes who achieved at least the 3rd place in the sprint at the Korea Gangwon-Do elementary school track and field meetings during 2004 and 2005 were selected as subjects. The running motions performed by the subjects were recorded using two 8mm high speed cameras at the nominal speed of 100 frames per second. The Direct Linear Transformation technique was adopted from the beginning of filming to the final stage of data extraction. KWON 3D motion analysis package program was used to compute the 3 Dimensional coordinates, smoothing factor in which lowpass filtering method was used and cutoff frequency was 6.0 Hz. The movement patterns during foot touchdown and takeoff for the running stride were related with the biomechanical consideration. Within the limitations of this study it is concluded: In order to increase running velocity, several conditions must be fullfilled at the instant of leg touchdown and takeoff during the fatigue phase(350-360m). First, the body C.O.G(Center of Gravity) height should be raised at the instant of leg touchdown and takeoff during the fatigue phase. Second, the foot contact time should be shortened and the takeoff distance should be increased at the foot takeoff during the fatigue phase. Third, the shank angular velocity with respect to a transverse axis through the center of gravity should be increased during the leg touchdown and takeoff in the fatigue phase. Forth, the active landing style described as clawing the ground with the sole of the foot should be performed during the leg touchdown and takeoff in the fatigue phase) phase. Fifth, In order to increase running velocity in the fatigue phase while taking a slightly greater leg knee angle and body lean angle within the range of the subject's running motion during the fatigue phase would result in greater flight distance.

• Proceedings of the KWS Conference
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• 2010.05a
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• pp.65-65
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• 2010

In shipbuilding industry, welding position are usually flat and vertical position at the erection stage. Application of SAW and EGW for these positions makes it possible to achieve enhanced productivity and high quality. But owing to their large size and weight it is difficult to apply these techniques in short and narrow regions. To overcome this problem, our company developed light weight and compact size 4-axis welding carriage which perform 3D weaving. The purpose of this study is to explain the development and application of intelligent welding carriage using 3D weaving pattern that can fill a large amount of welds and thereby making it possible to achieve high quality of welding. This study shows 3D weaving pattern, development of weaving database, and skill of adaptive control response for the variable gap. Also, it shows the results of procedure qualification test for the AH-grade steel when applied to the intelligent welding carriage.

• Journal of Welding and Joining
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• v.28 no.2
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• pp.32-38
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• 2010

In shipbuilding industry, welding position are usually flat and vertical position at the erection stage. Application of SAW and EGW for these positions makes it possible to achieve enhanced productivity and high quality. But owing to their large size and weight it is difficult to apply these techniques in short and narrow regions. To overcome this problem, our company developed light weight and compact size 4-axis welding carriage which perform 3D weaving. The purpose of this study is to explain the development and application of intelligent welding carriage using 3D weaving pattern that can fill a large amount of welds and thereby making it possible to achieve high quality of welding. This study shows 3D weaving pattern, development of weaving database, and skill of adaptive control response for the variable gap. Also, it shows the results of procedure qualification test for the AH-grade steel when applied to the intelligent welding carriage.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.6 no.1
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• pp.1-8
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• 2012

Estimating algorithm of physical activity energy expenditure was implemented by using a tri-axial accelerometer motion detector of the SVM(Signal Vector Magnitude) of 3-axis(x, y, z). A total of 33 participants(15 males and 18 females) that performed walking and running on treadmill at 2 ~ 11 km/h speeds(each stage increase 1km/h). Algorithm for energy expenditure of physical activities were implemented with $VO_2$ consumption and SVM correlation between the data. Algorithm consists of three kinds and hip, wrist, waist and hip can be used to apply.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.7 no.3
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• pp.157-170
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• 2002

To examine the structure and distribution of the Keum River plume produced by continuous river discharge we carried out three-dimensional numerical model experiments with or without Coriolis force and tide. When Coriolis force is included but tide is not the model plume forms the clockwise circulation north of southern channel in the developing stage. As the plume expansion progresses the center of circulation moves to the southwest, with fuming the discharging axis of low-salinity water to the southwest from the mouth of southern channel. These results are explained mainly in terms of barotropic geostrophy by surface slope maintained with accumulated low-salinity(buoyant) water in front of the estuary mouth due to of offshore strong salinity front. When the M$_2$ tide is included the model plume extends farther to the northwest, forming large tongue-like salinity distribution. The tidally averaged surface flows of the offshore plume are mainly in geostrophic balance. These changes in plume distribution are explained in terms of low-salinity water advection by tidal excursion and active tidal mixing; the former supplies low salinity water to the north off the estuary mouth and the later increases mean sea level along the plume and surface salinity in northern shallow coastal area. The main features of observed Keum River plume(Lee et al., 1999; Choi et al., 1999), which showed the northwestward deflection of the plume axis and northward deepening of the plume thickness from the estuary mouth region, are well reproduced by the model in which tide is included.