• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1083-1088
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• 2012

The breeding value of marbling score in skeletal muscle is an important factor for evaluating beef quality. In the present study, we investigated proteins associated with the breeding value of the marbling score for bovine sirloin to select potential biomarkers to improve meat quality through comparative proteomic analysis. Proteins isolated from muscle were separated by two-dimensional gel electrophoresis. After analyzing images of the stained gel, seven protein spots for the high marbling score group were identified corresponding to changes in expression that were at least two-fold compared to the low marbling score group. Four spots with increased intensities in the high marbling score group were identified as phosphoglycerate kinase 1, triosephophate isomerase, acidic ribosomal phosphoprotein PO, and capping protein (actin filament) Z-line alpha 2. Spots with decreased intensities in the high marbling score group compared to the low score group were identified as 14-3-3 epsilon, carbonic anhydrase II, and myosin light chain 1. Expression of myosin light chain 1 and carbonic anhydrase 2 was confirmed by Western blotting. Taken together, these data could help improve the economic performance of cattle and provide useful information about the underlying the function of bovine skeletal muscle.

• Geomechanics and Engineering
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• 제13권3호
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• pp.505-515
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• 2017

Based on the collapse characteristics of a shallow rectangular cavity, a three-dimensional failure mechanism which can be used to study the collapsing region of the rock mass above a shallow cavity roof is constructed. Considering the effects of surcharge pressure and surface bolt on the collapsing block, the external rate of works produced by surcharge pressure and surface bolt are included in the energy dissipation calculation. Using variational approach, an analytic expression of surface equation for the collapsing block, which can be used to study the collapsing region of the rock mass above a shallow cavity roof, is derived in the framework of upper bound theorem. Based on the analytic expression of surface equation, the shape of the collapsing block for shallow cavity is drawn. Moreover, the changing law of the collapsing region for different parameters indicates that the collapsing region of rock mass decreases with the increase of the density of surface bolt. This conclusion can provide reference for practicing geotechnical engineers to achieve an optimal design of supporting structure for a shallow cavity.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2004년도 가을 학술발표논문집 Vol.31 No.2 (2)
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• pp.748-750
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• 2004

본 논문은 연속적으로 입력되는 2차원 얼굴 영상에서 얼굴의 특징 영역들을 추출하여 3차원 얼굴 모델의 표정을 실시간으로 제어하는 방법에 관한 연구이다. 2차원 얼굴 영상에서 얼굴을 추출하기 위해 Hue, Saturation 색상 값을 사용하며, 두 가지 색상 값을 이용하여 피부색과 배경색을 분리함으로써 얼굴 영역을 추출 할 수 있다. 추출 된 얼굴에서 특징 영역인 눈 코, 입술 영역 등의 일지를 각각의 영역에 적합한 추출 방법을 이용하여 추출한 뒤, 프레임 별로 영역들의 움직임을 비교함으로써 영역의 움직임 정보를 획득 할 수 있다. 이 정보를 3차원 얼굴 모델에 적용하여 2차원 동영상에서 획득된 대상의 얼굴의 표정을 3차원 얼굴 모델에 실시간으로 표현 할 수 있도록 한다.

• 한국컴퓨터정보학회논문지
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• 제6권2호
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• pp.14-19
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• 2001

본 논문은 2차 다항식을 이용하여 3차원 물체의 표면 특징을 추출하고 표현하는 방법을 제안한다. 우리는 수정된 스캔 라인 기법을 이용하여 에지 맵을 얻는다. 에지 맵으로부터 3차원 물체의 각 면들을 분리하기 위해 레이블링 연산을 하고 각 면에서 중심점과 모서리 점들을 추출한다. 그 다음에, 평면 방정식으로부터 각 면이 평면인지 곡면인지를 판단하고, 3차원 물체를 표현하기 위해 각 면의 평면 또는 곡면의 계수 및 특징들을 추출한다. 그리고 합성영상과 실측영상을 통해서 제안된 기법의 성능을 알아보았다.

• Molecules and Cells
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• 제42권7호
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• pp.512-522
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• 2019

Chromosomes located in the nucleus form discrete units of genetic material composed of DNA and protein complexes. The genetic information is encoded in linear DNA sequences, but its interpretation requires an understanding of three-dimensional (3D) structure of the chromosome, in which distant DNA sequences can be juxtaposed by highly condensed chromatin packing in the space of nucleus to precisely control gene expression. Recent technological innovations in exploring higher-order chromatin structure have uncovered organizational principles of the 3D genome and its various biological implications. Very recently, it has been reported that large-scale genomic variations may disrupt higher-order chromatin organization and as a consequence, greatly contribute to disease-specific gene regulation for a range of human diseases. Here, we review recent developments in studying the effect of structural variation in gene regulation, and the detection and the interpretation of structural variations in the context of 3D chromatin structure.

• 복식
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• 제50권7호
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• pp.75-96
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• 2000

Modern clothing is approached in the level of art, and it becomes the means of expressing individuality in modern society uniformed by the development of information society. In this modern society, modern men need the recovery of humanity and expectations of handcrafted skills for the succession of tradition culture. Prior to expressing individuality, we should examine our traditional culture and combine it with western culture. Quit started to be used for practical purpose such as life items, but it is expanded to the fields of art. Used in dress and its ornaments design, quilt is often applied to the addition of aesthetic factors or cubic material feelings by transforming its warming effect. Hereby, this study has a purpose to create high value added modern dross and its ornaments design by expressing the modern clothing is approached in the level of art, and it becomes the means of expressing individuality roe-dimensional characteristics of quilt, and applying the color combination and the surface of butterfly wings to dress and its ornaments design. The following are the results of this study. 1. The concept of quilt started for practical purposes, but it is expanded to decoration in modern times, and it is confirmed that quilt can be variously applied to handcrafted modern design. 2. Quilt removes the plane character of textile and it riches the three-dimensional material of dress and its ornaments. Applying these characteristics, the expression of transparent wings were possible with 3 transparent layers of textile. 3. The spledid color of butterfly wings are expressed by coloring oganza and felt, and the various colors of felt showed rich color gradation. 4. The form and pattern of butterfly wings are applied as modeling form and line, and the transformation. repetition and expansion of unit forms determined the form of quilting lines. By designing the characteristics of back wings for the composition line of clothing, the form characteristics of a motif could be emphasized. 5. By using felt, oganza, Damdam yarn and ostritch feathers in expressing butterfly wings, the warm material of Linbun is felt, and the tip hair of wings are expressed by croche techniques using Damdam yarn.

• 복식문화연구
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• 제29권3호
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• pp.374-387
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• 2021

The purpose of this study is to develop up-cycling fashion design methods centered on discarded denim material for the study of original up-cycling design methods. Up-cycling fashion design work was developed using digital clothing technology. This is a recent hot topic among sustainable fashion design methods. Up-cycling fashion design expression methods (categorized as dismantlement, collages, dépaysement, grafting, weaving, and tearing) were centered on design methods. These methods create various three-dimensional modeling effects in planar forms, whereby five pieces can be applied to the fabric and digitally produced. The results are as follows: First, the use of discarded denim fabric for the development of up-cycling fashion design pieces enabled the recycling of existing resources, provided solutions to environmental pollution problems, and provided expansion opportunities for design processes for sustainable fashion products that expand the design value of denim products and their utility. Second, new eco-friendly fashion designs that attempt to achieve diversity in modern fashion trends could be presented through formative contemporary fashion produced by up-cycling work products. Third, up-cycling fashion design work is expected to provide opportunities for eco-friendly fashion design methods. This will expand the value of sustainable fashion design by recycling simple waste materials through the use of three-dimensional digital clothing technology and further through the presentation of expanded life cycles that extend product planning, production, and life cycles.

• Applied Microscopy
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• 제48권3호
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• pp.55-61
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• 2018

The synaptic vesicle is a specialized structure in presynaptic terminals that stores various neurotransmitters. The actin filament has been proposed for playing an important role in mobilizing synaptic vesicles. To understand the role of actin filament on synaptic vesicle pooling, we characterized synaptic vesicles and actin filament after treatment of brain-derived neurotrophic factor (BDNF) or Latrunculin A on primary cultured neuron from rat embryo hippocampus. Western blots revealed that BDNF treatment increased the expression of synapsin I protein, but Latrunculin A treatment decreased the synapsin I protein expression. The increased expression of synapsin I after BDNF disappeared by the treatment of Latrunculin A. Three-dimensional (3D) tomography of synapse showed that more synaptic vesicles localized near the active zone and total number of synaptic vesicles increased after treatment of BDNF. But the number of synaptic vesicle was 2.5-fold reduced in presynaptic terminals and the loss of filamentous network was observed after Latrunculin A application. The treatment of Latruculin A after preincubation of BDNF group showed that synaptic vesicle number was similar to that of control group, but filamentous structures were not restored. These data suggest that the actin filament plays a significant role in synaptic vesicles pooling in presynaptic terminals.

• Journal of Ginseng Research
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• 제45권1호
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• pp.58-65
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• 2021

Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

• 한국정보통신학회논문지
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• 제20권11호
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• pp.2021-2026
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• 2016

본 논문에서는 3차원 신호의 전송을 위한 시스템 모델을 제시하고 성능을 분석한다. 2차원 신호와는 달리 3차원 신호를 표현할 수 있는 직교형식의 부재로 인하여 여기서는 직교 기저함수를 이용하여 3차원 신호를 전송한다. 초고 차레벨 격자형 신호성상도를 이용한 모의실험 결과, 3차원 전송시스템은 2차원 시스템에 비하여 현저히 향상된 오류 성능을 가지는 것으로 확인되었다. 이러한 성능 향상은 3차원 격자형 신호성상도의 심볼간 최소 유클리드 거리가 직교진폭변조 성상도에 비하여 훨씬 증가된 것이 주요 원인인 것으로 판단된다. 이를 확인하기 위하여 최소 유클리드 거리를 비교한 결과, 3차원 1024진 성상도는 직교진폭변조 성상도에 비하여 약 2.6배 증가된 최소 유클리드 거리를 가지는 것으로 나타났다. 또한 4096진 성상도의 경우 3차원 성상도의 최소 유클리드 거리는 2차원 성상도에 비하여 약 3.2배 증가하였다.